BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146792.8 - phase: 0
(266 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g21450 unknown protein 363 e-101
At4g05060 unknown protein 340 4e-94
At5g54110 membrane associated protein 321 2e-88
At3g60600 unknown protein 60 2e-09
At2g45140 putative VAMP-associated protein 56 2e-08
At4g00175 putative proline-rich protein 54 9e-08
At1g51270 unknown protein 52 3e-07
At2g23830 unknown protein 46 2e-05
At5g47180 VAMP (vesicle-associated membrane protein)-associated ... 45 4e-05
At1g08820 unknown protein 45 5e-05
At1g64050 unknown protein 36 0.024
At1g50600 scarecrow-like 5 (SCL5) 35 0.054
At3g58120 bZip transcription factor AtbZip61 34 0.071
At2g23320 putative WRKY-type DNA-binding protein 34 0.092
At5g14520 pescadillo - like protein 33 0.12
At5g01310 putative protein 33 0.12
At5g18910 protein kinase - like protein 33 0.16
At4g23060 unknown protein 33 0.16
At3g61570 unknown protein 33 0.21
At2g05940 putative protein kinase 33 0.21
>At4g21450 unknown protein
Length = 295
Score = 363 bits (931), Expect = e-101
Identities = 196/290 (67%), Positives = 223/290 (76%), Gaps = 30/290 (10%)
Query: 1 MAVAEPKLHSEPKV-WNFFKLPFRNSN-----------------TSSTNTTSSSVNNNLH 42
M + E K S+ K W FFK+PFRNS+ SS++T+S N++ H
Sbjct: 1 MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPSGASSSSTSSHLHNHHQH 60
Query: 43 HH-----HHHNPNSNTPL---EGSTSHT--SNSVSSVARSLLPTRRRLKLDPSNKLYFPY 92
HH HHH N P G H S SVSSVA+S LPT+RRLKLDPS KLYFPY
Sbjct: 61 HHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPY 120
Query: 93 EPGKQVRSAIRIKNTSKSNVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKFVEQPEN 152
EPGKQVRSAI+IKNTSKS+VAFKFQTTAPKSCFMRPPGAILAPGE+IIATVFKFVE PEN
Sbjct: 121 EPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPEN 180
Query: 153 NEKP--EKSGLKFKIMSLKVKGSIDYVPELFDEQKDQVAVEQILRVVFLDPERPSPILEK 210
NEKP ++S +KFKIMSLKVKG +DYVPELFDEQKD V+ EQILRV+FLDPER +P LEK
Sbjct: 181 NEKPMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEK 240
Query: 211 LKRQLADADAALEERKKPAEDAGPKIIGEGLVIDEWKERRERYLAKQQGD 260
LKRQLA+ADAA+E RKKP E+ GPK+IGEGLVIDEWKERRERYLA+QQG+
Sbjct: 241 LKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRERYLAQQQGE 290
>At4g05060 unknown protein
Length = 287
Score = 340 bits (873), Expect = 4e-94
Identities = 187/284 (65%), Positives = 213/284 (74%), Gaps = 23/284 (8%)
Query: 1 MAVAEPKLHSEPKVWNFFKLPFRNSN-------------TSSTNTTSSSVNNNLHHHHHH 47
MA+ E K S+ + W FKLPFRNSN TSS++ TSS +N N H H
Sbjct: 1 MALTEDKSDSDGRRWGKFKLPFRNSNSQAPSASSSSSMATSSSSVTSSHLNQNYIHQSRH 60
Query: 48 NPNSNTPLE---GSTSHTSN----SVSSVARSLLPTRRRLKLDPSNKLYFPYEPGKQVRS 100
P+ G H S S+SSVARSLLPT+RRLKLDPS KLYFPYEPGKQVRS
Sbjct: 61 FQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLKLDPSAKLYFPYEPGKQVRS 120
Query: 101 AIRIKNTSKSNVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFKFVEQPENNEKP--EK 158
AI+IKNTSKS+VAFKFQTT PKSCFMRP GAILAPGE IIATVFKFVE PENNEKP +K
Sbjct: 121 AIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEIIATVFKFVEPPENNEKPMEQK 180
Query: 159 SGLKFKIMSLKVKGSIDYVPELFDEQKDQVAVEQILRVVFLDPERPSPILEKLKRQLADA 218
SG+KFKIMSLK+K DY+PELF+EQKD V+ EQ++RVVFLDPE P+ ++EKLK QLA+A
Sbjct: 181 SGVKFKIMSLKMKVPTDYMPELFEEQKDHVSEEQVMRVVFLDPENPNSMMEKLKSQLAEA 240
Query: 219 DAALEERKKPAED-AGPKIIGEGLVIDEWKERRERYLAKQQGDV 261
DAA E RKK +E GPK IGEGLVIDEWK+RRERYLA+QQG V
Sbjct: 241 DAADEARKKASEGIVGPKPIGEGLVIDEWKQRRERYLAQQQGGV 284
>At5g54110 membrane associated protein
Length = 266
Score = 321 bits (823), Expect = 2e-88
Identities = 169/266 (63%), Positives = 208/266 (77%), Gaps = 8/266 (3%)
Query: 1 MAVAEPKLHSEPKVWNFFKL-PFRNSNTSSTNTTSSSVNNNLHHHHHHNPNSNTPLEGST 59
M + + + S K N F+L PF ST ++SS+ N N ++ H N NT + +
Sbjct: 1 MPIGDRQNPSVEKKKNLFRLCPFWQRR--STTSSSSTQNPNQNYRSRHG-NRNTDIS-AV 56
Query: 60 SHTSNSVSSVARSLLPTRRRLKLDPSNKLYFPYEPGKQVRSAIRIKNTSKSNVAFKFQTT 119
S ++SSVARSLLP RRRL+LDPS+ LYFPYEPGKQVRSAI++KNTSKS+ AFKFQTT
Sbjct: 57 SKPPLTMSSVARSLLPARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTT 116
Query: 120 APKSCFMRPPGAILAPGESIIATVFKFVEQPENNEKP---EKSGLKFKIMSLKVKGSIDY 176
APKSC+MRPPG +LAPGES+ ATVFKFVE PENNEK +KS +KFKIMSLKVK ++Y
Sbjct: 117 APKSCYMRPPGGVLAPGESVFATVFKFVEHPENNEKQPLNQKSKVKFKIMSLKVKPGVEY 176
Query: 177 VPELFDEQKDQVAVEQILRVVFLDPERPSPILEKLKRQLADADAALEERKKPAEDAGPKI 236
VPELFDEQKDQVAVEQ+LRV+F+D +RPS LEKLKRQL +A+AA+E RKKP + GP++
Sbjct: 177 VPELFDEQKDQVAVEQVLRVIFIDADRPSAALEKLKRQLDEAEAAVEARKKPPPETGPRV 236
Query: 237 IGEGLVIDEWKERRERYLAKQQGDVV 262
+GEGLVIDEWKERRE+YLA+QQ + V
Sbjct: 237 VGEGLVIDEWKERREKYLARQQVESV 262
>At3g60600 unknown protein
Length = 256
Score = 59.7 bits (143), Expect = 2e-09
Identities = 40/125 (32%), Positives = 66/125 (52%), Gaps = 4/125 (3%)
Query: 88 LYFPYEPGKQVRSAIRIKNTSKSNVAFKFQTTAPKSCFMRPPGAILAPGES--IIATVFK 145
L FP+E KQ+ ++ + N + +NVAFK +TT PK +RP ++ P + ++ T+
Sbjct: 30 LQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTCEVLVTMQA 89
Query: 146 FVEQPENNEKPEKSGLKFKIMSLKVKGSIDYVPELFDEQKDQVAVEQILRVVFLDPER-P 204
E P + + +K L+ I S V + PE+F ++ E LRV ++ P R P
Sbjct: 90 QKEAPSDMQCKDKFLLQGVIASPGVTAK-EVTPEMFSKEAGHRVEETKLRVTYVAPPRPP 148
Query: 205 SPILE 209
SP+ E
Sbjct: 149 SPVHE 153
>At2g45140 putative VAMP-associated protein
Length = 239
Score = 55.8 bits (133), Expect = 2e-08
Identities = 42/133 (31%), Positives = 69/133 (51%), Gaps = 5/133 (3%)
Query: 80 LKLDPSNKLYFPYEPGKQVRSAIRIKNTSKSNVAFKFQTTAPKSCFMRPPGAILAPGES- 138
L +DP + L FP+E KQ+ ++ + N + + VAFK +TT PK +RP ++ P S
Sbjct: 6 LTIDPVD-LQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPRSSS 64
Query: 139 -IIATVFKFVEQPENNEKPEKSGLKFKIMSLKVKGSIDYVPELFDEQKDQVAVEQILRVV 197
++ T+ E P + + +K L+ + S D E+F ++ E LRVV
Sbjct: 65 EVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPK-DVTHEMFSKEAGHRVEETKLRVV 123
Query: 198 FLDPER-PSPILE 209
++ P R PSP+ E
Sbjct: 124 YVAPPRPPSPVRE 136
>At4g00175 putative proline-rich protein
Length = 239
Score = 53.9 bits (128), Expect = 9e-08
Identities = 38/127 (29%), Positives = 68/127 (52%), Gaps = 6/127 (4%)
Query: 87 KLYFPYEPGKQVRSAIRIKN-TSKSNVAFKFQTTAPKSCFMRPPGAILAPGESIIATVFK 145
+L FP+E KQ ++++ N T+ VAFK +TT P+ +RP ++ PG+S TV
Sbjct: 13 ELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDSCNVTVTM 72
Query: 146 FVEQPENNEKPEKSGLKFKIMSLKVKG---SIDYVPELFDEQKDQVAVEQILRVVFLDPE 202
++ + K KF + ++ V S + + E+F+++ +V + LRVV++
Sbjct: 73 QAQKEAPLDMQCKD--KFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVVYIPAN 130
Query: 203 RPSPILE 209
PSP+ E
Sbjct: 131 PPSPVPE 137
>At1g51270 unknown protein
Length = 289
Score = 52.4 bits (124), Expect = 3e-07
Identities = 36/133 (27%), Positives = 70/133 (52%), Gaps = 5/133 (3%)
Query: 80 LKLDPSNKLYFPYEPGKQVRSAIRIKNTSKSNVAFKFQTTAPKSCFMRPPGAILAPGES- 138
L +DP + + FP E K+V ++ + N +++ VAFK +TT K ++RP ++ P S
Sbjct: 129 LIIDPVD-VQFPIELNKKVSCSLNLTNKTENYVAFKAKTTNAKKYYVRPNVGVVLPRSSC 187
Query: 139 -IIATVFKFVEQPENNEKPEKSGLKFKIMSLKVKGSIDYVPELFDEQKDQVAVEQILRVV 197
++ + E P + + +K + K++ + D E+F ++ A E L+V+
Sbjct: 188 EVLVIMQALKEAPADMQCRDKLLFQCKVVEPETTAK-DVTSEMFSKEAGHPAEETRLKVM 246
Query: 198 FL-DPERPSPILE 209
++ P+ PSP+ E
Sbjct: 247 YVTPPQPPSPVQE 259
>At2g23830 unknown protein
Length = 149
Score = 45.8 bits (107), Expect = 2e-05
Identities = 33/122 (27%), Positives = 63/122 (51%), Gaps = 4/122 (3%)
Query: 80 LKLDPSNKLYFPYEPGKQVRSAIRIKNTSKSNVAFKFQTTAPKSCFMRPPGAILAPGES- 138
L+++P L FP+E KQ+ ++ + N + +NVAFK +TT + +RP ++ P +
Sbjct: 7 LEIEPMY-LQFPFELKKQMSCSLYLTNKTDNNVAFKVKTTNRNNYCVRPNYGLILPKSTC 65
Query: 139 -IIATVFKFVEQPENNEKPEKSGLKFKIMSLKVKGSIDYVPELFDEQKDQVAVEQILRVV 197
++ T+ E P + + EK ++ + S V + E+F ++ V E LRV
Sbjct: 66 KVLVTMQAQKEVPSDMQSFEKFMIQSVLASPGVTAK-EVTREMFSKESGHVVEETKLRVT 124
Query: 198 FL 199
++
Sbjct: 125 YV 126
>At5g47180 VAMP (vesicle-associated membrane protein)-associated
protein-like
Length = 220
Score = 45.1 bits (105), Expect = 4e-05
Identities = 33/119 (27%), Positives = 62/119 (51%), Gaps = 5/119 (4%)
Query: 86 NKLYFPYEPGKQVRSAIRIKNTSKSNVAFKFQTTAPKSCFMRPPGAILAPGESII--ATV 143
++L F +E KQ +++ N +++ VAFK +TT+PK F+RP ++ P +S I T+
Sbjct: 15 DELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDSCIIRVTL 74
Query: 144 FKFVEQPENNEKPEKSGLKFKIMSLKVKGSIDYVP-ELFDEQKDQVAVEQILRVVFLDP 201
E P + + +K L+ I+ +D +P + F + + E L+V ++ P
Sbjct: 75 QAQREYPPDMQCKDKFLLQSTIVPPHT--DVDELPQDTFTKDSGKTLTECKLKVSYITP 131
>At1g08820 unknown protein
Length = 386
Score = 44.7 bits (104), Expect = 5e-05
Identities = 39/143 (27%), Positives = 66/143 (45%), Gaps = 7/143 (4%)
Query: 88 LYFPYEPGKQVRSAIRIKNTSKSNVAFKFQTTAPKSCFMRPPGAILAPGESIIATVF--K 145
L F + KQ +++ NT+ VAFK +TT+PK +RP ++AP + TV
Sbjct: 13 LQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAPKSTCEFTVIMQA 72
Query: 146 FVEQPENNEKPEKSGLKFKIMSLKVKGSIDYVPELFDEQKDQVAVEQILRVVFLDPERPS 205
F E P + +K ++ +S + D +F + + + E LRV + P S
Sbjct: 73 FKEPPPDMVCKDKFLIQSTAVSAETTDE-DITASMFSKAEGKHIEENKLRVTLVPPS-DS 130
Query: 206 PILEKL---KRQLADADAALEER 225
P L + K+ D+ L++R
Sbjct: 131 PELSPINTPKQGAVFEDSILKDR 153
>At1g64050 unknown protein
Length = 668
Score = 35.8 bits (81), Expect = 0.024
Identities = 19/72 (26%), Positives = 33/72 (45%), Gaps = 20/72 (27%)
Query: 24 NSNTSSTNTTSSSVNNNLHHHHHHN-------------------PNSNTPLEGSTSHTSN 64
N+N + + S + HHHHHH+ P+S++P+E H+S+
Sbjct: 575 NNNNNKNHVRKDSAAEHNHHHHHHHPKPSKRLKLSTMENKKRSFPSSSSPIESDRKHSSS 634
Query: 65 S-VSSVARSLLP 75
S + +R +LP
Sbjct: 635 SKFKNHSRMMLP 646
>At1g50600 scarecrow-like 5 (SCL5)
Length = 597
Score = 34.7 bits (78), Expect = 0.054
Identities = 20/51 (39%), Positives = 30/51 (58%), Gaps = 10/51 (19%)
Query: 24 NSNTSSTNTTSSSV--------NNNLHHHHHHNP--NSNTPLEGSTSHTSN 64
N N SS+ T+ SS NNNL ++H+P N+N+PL GS++ +N
Sbjct: 124 NKNNSSSTTSFSSNESPISQANNNNLSRFNNHSPEENNNSPLSGSSATNTN 174
>At3g58120 bZip transcription factor AtbZip61
Length = 329
Score = 34.3 bits (77), Expect = 0.071
Identities = 14/26 (53%), Positives = 18/26 (68%)
Query: 38 NNNLHHHHHHNPNSNTPLEGSTSHTS 63
NNN +HHHHH+ N N S+S+TS
Sbjct: 102 NNNHNHHHHHSINGNVGPTRSSSNTS 127
>At2g23320 putative WRKY-type DNA-binding protein
Length = 317
Score = 33.9 bits (76), Expect = 0.092
Identities = 15/55 (27%), Positives = 26/55 (47%)
Query: 18 FKLPFRNSNTSSTNTTSSSVNNNLHHHHHHNPNSNTPLEGSTSHTSNSVSSVARS 72
F + + SS ++ ++ +N HHH P+ P T S +VSS ++S
Sbjct: 134 FSSSMKTIDFSSLSSVTTESDNQKKIHHHQRPSETAPFASQTQSLSTTVSSFSKS 188
>At5g14520 pescadillo - like protein
Length = 590
Score = 33.5 bits (75), Expect = 0.12
Identities = 22/97 (22%), Positives = 45/97 (45%), Gaps = 4/97 (4%)
Query: 135 PGESIIATVFKFVEQPENNEKPEKSGLKFKI----MSLKVKGSIDYVPELFDEQKDQVAV 190
P + A V E+ E + +K + K + +++ GS D V + E + + +V
Sbjct: 474 PQNLLYAGVMSRAEEAEAAKNKKKMAAQEKQYHEELKMEINGSKDVVAPVLAEGEGEESV 533
Query: 191 EQILRVVFLDPERPSPILEKLKRQLADADAALEERKK 227
+++ D + P ++ + KR+L DA + RK+
Sbjct: 534 PDAMQIAQEDADMPKVLMSRKKRKLYDAMKISQSRKR 570
>At5g01310 putative protein
Length = 912
Score = 33.5 bits (75), Expect = 0.12
Identities = 29/97 (29%), Positives = 40/97 (40%), Gaps = 9/97 (9%)
Query: 18 FKLPFRNSNTSSTNTTSSSVNNNLHHHHHHNPNSNTPLEGSTSHTSNSVSSVARSLLPTR 77
F L N N+SST ++SSS H H + NT STS S SVA
Sbjct: 4 FNLRSENPNSSSTTSSSSS-----SFHRHKSETGNTKRSRSTSTLSTDPQSVA----ARD 54
Query: 78 RRLKLDPSNKLYFPYEPGKQVRSAIRIKNTSKSNVAF 114
RR ++ K+ PG + + + + S V F
Sbjct: 55 RRHRISDRFKILQSMVPGGAKMDTVSMLDEAISYVKF 91
>At5g18910 protein kinase - like protein
Length = 511
Score = 33.1 bits (74), Expect = 0.16
Identities = 12/30 (40%), Positives = 20/30 (66%)
Query: 28 SSTNTTSSSVNNNLHHHHHHNPNSNTPLEG 57
+S++ S+S ++ HHHHHH+ +S P G
Sbjct: 86 TSSSVVSTSDSSETHHHHHHHHSSGGPNNG 115
Score = 29.6 bits (65), Expect = 1.7
Identities = 11/22 (50%), Positives = 15/22 (68%)
Query: 27 TSSTNTTSSSVNNNLHHHHHHN 48
+SS +TS S + HHHHHH+
Sbjct: 87 SSSVVSTSDSSETHHHHHHHHS 108
>At4g23060 unknown protein
Length = 484
Score = 33.1 bits (74), Expect = 0.16
Identities = 23/60 (38%), Positives = 30/60 (49%), Gaps = 1/60 (1%)
Query: 30 TNTTSSSVNNNLHHHHHHNPNSNTPLEGSTSHTSNSVSSVARSLLPTRRRLKLDPSNKLY 89
T +SSS +NN H NP TP + S +S S S +A S L R K +N+LY
Sbjct: 233 TPESSSSQSNNTKSSHFQNPGPPTPEKLEHSISSRS-SKLAHSHLFKRNGSKASDNNRLY 291
>At3g61570 unknown protein
Length = 712
Score = 32.7 bits (73), Expect = 0.21
Identities = 17/42 (40%), Positives = 21/42 (49%)
Query: 208 LEKLKRQLADADAALEERKKPAEDAGPKIIGEGLVIDEWKER 249
L KL +L D+D LE K ED K++ V EWK R
Sbjct: 492 LMKLSARLKDSDERLESSNKEKEDVTSKLLHAEKVAAEWKNR 533
>At2g05940 putative protein kinase
Length = 462
Score = 32.7 bits (73), Expect = 0.21
Identities = 17/55 (30%), Positives = 23/55 (40%), Gaps = 1/55 (1%)
Query: 18 FKLPFRNSNTSSTNTTSSSVNNNLHHHHHHNPNSNTPLEGSTSHTSNSVSSVARS 72
+ +P N + + HHHHH SN P S S T+ S S A+S
Sbjct: 380 YTVPNTPDNKEDDGRVGNKPRKSSHHHHHQQQQSNHP-RSSPSPTTKSPSPTAKS 433
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.313 0.130 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,241,530
Number of Sequences: 26719
Number of extensions: 271305
Number of successful extensions: 1894
Number of sequences better than 10.0: 126
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 51
Number of HSP's that attempted gapping in prelim test: 1512
Number of HSP's gapped (non-prelim): 263
length of query: 266
length of database: 11,318,596
effective HSP length: 98
effective length of query: 168
effective length of database: 8,700,134
effective search space: 1461622512
effective search space used: 1461622512
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146792.8