BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146784.1 - phase: 0
(151 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g56400 putative DNA-binding protein 74 2e-14
At2g40750 unknown protein 61 3e-10
At2g46400 putative WRKY-type DNA binding protein 58 2e-09
At5g22570 WRKY transcription factor 38 (WRKY38) 54 2e-08
At4g11070 WRKY transcription factor 41 (WRKY41) 54 2e-08
At5g01900 WRKY DNA-binding protein 62 (WRKY62) 49 8e-07
At1g69310 putative WRKY transcription factor 49 8e-07
At5g24110 unknown protein 49 1e-06
At1g66550 WRKY transcription factor 67 (WRKY67) 48 2e-06
At2g03340 putative WRKY DNA-binding protein 45 1e-05
At1g13960 WRKY DNA-binding protein 4 (WRKY4) 43 6e-05
At5g46350 putative protein 43 7e-05
At2g47260 putative WRKY-type DNA binding protein 43 7e-05
At2g40740 putative WRKY-type DNA binding protein 42 1e-04
At1g66560 putative protein 42 1e-04
At4g18170 DNA binding like protein 41 2e-04
At3g01080 WRKY transcription factor 58 (WRKY58) 41 3e-04
At3g01970 putative WRKY-like transcriptional regulator protein 40 4e-04
At1g29860 putative protein 40 4e-04
At5g56270 transcription factor NtWRKY4-like 40 5e-04
>At3g56400 putative DNA-binding protein
Length = 294
Score = 74.3 bits (181), Expect = 2e-14
Identities = 52/143 (36%), Positives = 74/143 (51%), Gaps = 25/143 (17%)
Query: 14 KVIDELRRGRELANQLKNILNESGDFDDINGSTTPFAEDLLKEVLTTFTNSLLFLNN-NP 72
KV+++L G +L QL+ +L++ G EDL+ ++L F N++ L+ P
Sbjct: 10 KVMNQLVEGHDLTTQLQQLLSQPGSG----------LEDLVAKILVCFNNTISVLDTFEP 59
Query: 73 TSEGSDMQLT------------KSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDR 120
S S + K EDS +S K VK +RGCYKR+K S+T ES
Sbjct: 60 ISSSSSLAAVEGSQNASCDNDGKFEDSGDSR-KRLGPVKGKRGCYKRKKRSETCTIESTI 118
Query: 121 PVEDGHQWRKYGQKKILHTDFPR 143
+ED WRKYGQK+IL+ FPR
Sbjct: 119 -LEDAFSWRKYGQKEILNAKFPR 140
>At2g40750 unknown protein
Length = 346
Score = 60.8 bits (146), Expect = 3e-10
Identities = 51/161 (31%), Positives = 78/161 (47%), Gaps = 33/161 (20%)
Query: 14 KVIDELRRGRELANQLKNILNES-------------GDFDDINGSTTPFAEDLLKEVLTT 60
KV+D+L G E A QL+ +L+ ++G P ++L+ ++L +
Sbjct: 14 KVVDQLVEGYEFATQLQLLLSHQHSNQYHIDETRLVSGSGSVSGGPDP-VDELMSKILGS 72
Query: 61 FTNSLLFLNN-NPTS-------EGS------DMQLT----KSEDSLESNCKSTSIVKERR 102
F ++ L++ +P + EGS D T DS ES K + K +R
Sbjct: 73 FHKTISVLDSFDPVAVSVPIAVEGSWNASCGDDSATPVSCNGGDSGESKKKRLGVGKGKR 132
Query: 103 GCYKRRKVSQTWEKESDRPVEDGHQWRKYGQKKILHTDFPR 143
GCY R+ S T E+ + ED + WRKYGQK+IL+T FPR
Sbjct: 133 GCYTRKTRSHTRIVEA-KSSEDRYAWRKYGQKEILNTTFPR 172
>At2g46400 putative WRKY-type DNA binding protein
Length = 295
Score = 58.2 bits (139), Expect = 2e-09
Identities = 40/141 (28%), Positives = 67/141 (47%), Gaps = 30/141 (21%)
Query: 15 VIDELRRGRELANQLKNILNESGDFDDINGSTTPFAEDLLKEVLTTFTNSLLFLNNNPTS 74
VI+EL G+ELAN+L N L + D + L+ ++L + N++ L+ N
Sbjct: 8 VINELELGKELANRLMNNLKHTSSVDS--------NKTLISDILRIYQNAIFMLSFN--- 56
Query: 75 EGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQ---------TWEKESDRPVEDG 125
D + K ++ K+ + +K+RKVS+ ++ + ++DG
Sbjct: 57 --QDKNILKRSLEIDG--------KDSKNVFKKRKVSEKNTEKVKVFVATEQENGSIDDG 106
Query: 126 HQWRKYGQKKILHTDFPRYIY 146
H WRKYGQK+I + PR Y
Sbjct: 107 HCWRKYGQKEIHGSKNPRAYY 127
>At5g22570 WRKY transcription factor 38 (WRKY38)
Length = 289
Score = 54.3 bits (129), Expect = 2e-08
Identities = 42/145 (28%), Positives = 65/145 (43%), Gaps = 22/145 (15%)
Query: 14 KVIDELRRGRELANQLKNILNES-GDFDDINGSTTPFAEDLLKEVLTTFTNSLLFLNNNP 72
K + +R G A +LK LN D ++ + DL K ++ +F+N++ L+ P
Sbjct: 9 KAVQAIRYGHSCAMRLKRRLNHPMADGGPLS------SYDLAKSIVESFSNAISILSAKP 62
Query: 73 TSEGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKV-----SQTWEKESDRPVE-DGH 126
+E S DS S S K+RK+ S+ W +S P+ DG+
Sbjct: 63 ETEDDQFSDLSSRDSSPPPQGSPS---------KKRKIDSTNSSENWRDDSPDPIYYDGY 113
Query: 127 QWRKYGQKKILHTDFPRYIYFYEFN 151
WRKYGQK I ++ R Y +N
Sbjct: 114 LWRKYGQKSIKKSNHQRSYYRCSYN 138
>At4g11070 WRKY transcription factor 41 (WRKY41)
Length = 313
Score = 54.3 bits (129), Expect = 2e-08
Identities = 45/158 (28%), Positives = 69/158 (43%), Gaps = 24/158 (15%)
Query: 12 RGKVIDELRRGRELANQLKNILNESGDFDDINGSTTPFAEDLLKEVLTTFTNSLLFLNNN 71
R +++EL G + A QL+ + S + TT E+LL ++++F ++L LN +
Sbjct: 8 RRSLLNELIHGLKAAKQLQGSSSPSLSASS-SYLTTEIKENLLHNIVSSFKKAILMLNGS 66
Query: 72 PTSEGSDMQLTK-----------SEDSLESNCKSTSIV----KERRGCYKRRKVSQTW-- 114
T ++L S S+ N +S KE K+RK+ W
Sbjct: 67 TTQHNPTIELAPDPLAHPGKVPGSPASITGNPRSEEFFNVRSKEFNLSSKKRKMLPKWTE 126
Query: 115 ------EKESDRPVEDGHQWRKYGQKKILHTDFPRYIY 146
E+ + P +D WRKYGQK IL FPR Y
Sbjct: 127 QVRISPERGLEGPHDDIFSWRKYGQKDILGAKFPRSYY 164
>At5g01900 WRKY DNA-binding protein 62 (WRKY62)
Length = 263
Score = 49.3 bits (116), Expect = 8e-07
Identities = 41/144 (28%), Positives = 61/144 (41%), Gaps = 28/144 (19%)
Query: 14 KVIDELRRGRELANQLKNILNESGDFDDINGSTTPFAEDLLKEVLTTFTNSLLFL----- 68
K +++L G ANQL + D ++ EDL K VL F+++L L
Sbjct: 7 KAMEKLLHGHGCANQLLIM-------DQTESDSSMEREDLAKSVLHCFSDALSILIDTND 59
Query: 69 -----NNNPTSEGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPV- 122
+NN + + S L S L + TS+ + S ES P+
Sbjct: 60 HQDDQSNNSSPQDSSPVLESSRKPLHKRGRKTSMAES----------SDYHRHESSTPIY 109
Query: 123 EDGHQWRKYGQKKILHTDFPRYIY 146
DG WRKYGQK+I +++ R Y
Sbjct: 110 HDGFLWRKYGQKQIKESEYQRSYY 133
>At1g69310 putative WRKY transcription factor
Length = 287
Score = 49.3 bits (116), Expect = 8e-07
Identities = 32/82 (39%), Positives = 45/82 (54%), Gaps = 4/82 (4%)
Query: 69 NNNP--TSEGSDMQLTKSEDSLESNCKSTSIVKERRGCYKR-RKVSQTWEKESDRP-VED 124
NNNP TS S+ S S E + VKE++ KR R+ + +SD +ED
Sbjct: 89 NNNPSATSSSSEDPAENSTASAEKTPPPETPVKEKKKAQKRIRQPRFAFMTKSDVDNLED 148
Query: 125 GHQWRKYGQKKILHTDFPRYIY 146
G++WRKYGQK + ++ FPR Y
Sbjct: 149 GYRWRKYGQKAVKNSPFPRSYY 170
>At5g24110 unknown protein
Length = 303
Score = 48.9 bits (115), Expect = 1e-06
Identities = 38/137 (27%), Positives = 61/137 (43%), Gaps = 26/137 (18%)
Query: 24 ELANQLKNILNESGDFDDINGSTTP--FAEDLLKEVLTTFTNSLLFLN------------ 69
++ N++ ++ E D+ GS + E L K++L ++ SL +N
Sbjct: 12 KMKNEINELMIEGRDYAHQFGSASSQETREHLAKKILQSYHKSLTIMNYSGELDQVSQGG 71
Query: 70 NNPTSEGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPVEDGHQWR 129
+P S+ SD + + S +S + +S V+ G DR ++DG WR
Sbjct: 72 GSPKSDDSDQEPLVIKSSKKSMPRWSSKVRIAPGA------------GVDRTLDDGFSWR 119
Query: 130 KYGQKKILHTDFPRYIY 146
KYGQK IL FPR Y
Sbjct: 120 KYGQKDILGAKFPRGYY 136
>At1g66550 WRKY transcription factor 67 (WRKY67)
Length = 254
Score = 47.8 bits (112), Expect = 2e-06
Identities = 41/136 (30%), Positives = 62/136 (45%), Gaps = 15/136 (11%)
Query: 14 KVIDELRRGRELANQLKNILNESGDFDDINGSTTPFAEDLLKEVLTTFTNSLLFL---NN 70
K ++ L RG+ AN LK IL E+G+ ++ E L+ +L +F+ +L F+ N+
Sbjct: 8 KAMEALLRGQGCANNLK-ILLENGEISSVS------TEPLIHTILDSFSLALSFMDSPNH 60
Query: 71 NPTSEGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPVEDGHQWRK 130
P E S + S S S + R+ C V+ + + P DG WRK
Sbjct: 61 PPYHESSSHNMA----SHMSRRSSKQVQHRRKLCVAEGLVNYNHDSRTMCP-NDGFTWRK 115
Query: 131 YGQKKILHTDFPRYIY 146
YGQK I + R Y
Sbjct: 116 YGQKTIKASAHKRCYY 131
>At2g03340 putative WRKY DNA-binding protein
Length = 513
Score = 45.4 bits (106), Expect = 1e-05
Identities = 16/31 (51%), Positives = 24/31 (76%)
Query: 116 KESDRPVEDGHQWRKYGQKKILHTDFPRYIY 146
+ +D+P +DG+ WRKYGQK++ +DFPR Y
Sbjct: 243 QNADKPADDGYNWRKYGQKQVKGSDFPRSYY 273
Score = 34.3 bits (77), Expect = 0.026
Identities = 12/25 (48%), Positives = 18/25 (72%)
Query: 122 VEDGHQWRKYGQKKILHTDFPRYIY 146
++DG++WRKYGQK + +PR Y
Sbjct: 414 LDDGYRWRKYGQKVVKGNPYPRSYY 438
>At1g13960 WRKY DNA-binding protein 4 (WRKY4)
Length = 514
Score = 43.1 bits (100), Expect = 6e-05
Identities = 15/28 (53%), Positives = 22/28 (78%)
Query: 119 DRPVEDGHQWRKYGQKKILHTDFPRYIY 146
D+P +DG+ WRKYGQK++ ++FPR Y
Sbjct: 225 DKPADDGYNWRKYGQKQVKGSEFPRSYY 252
Score = 34.3 bits (77), Expect = 0.026
Identities = 12/25 (48%), Positives = 18/25 (72%)
Query: 122 VEDGHQWRKYGQKKILHTDFPRYIY 146
++DG++WRKYGQK + +PR Y
Sbjct: 408 LDDGYRWRKYGQKVVKGNPYPRSYY 432
>At5g46350 putative protein
Length = 326
Score = 42.7 bits (99), Expect = 7e-05
Identities = 23/63 (36%), Positives = 39/63 (61%), Gaps = 3/63 (4%)
Query: 86 DSLESNCKSTSIVKERRGCYKRR--KVSQTWEKESDRPVEDGHQWRKYGQKKILHTDFPR 143
D E + +S +VK ++ K++ +VS + E D +EDG++WRKYGQK + ++ +PR
Sbjct: 145 DGGEDDQRSQKVVKTKKKEEKKKEPRVSFMTKTEVDH-LEDGYRWRKYGQKAVKNSPYPR 203
Query: 144 YIY 146
Y
Sbjct: 204 SYY 206
>At2g47260 putative WRKY-type DNA binding protein
Length = 337
Score = 42.7 bits (99), Expect = 7e-05
Identities = 24/72 (33%), Positives = 38/72 (52%), Gaps = 6/72 (8%)
Query: 75 EGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPVEDGHQWRKYGQK 134
EG + Q KS + K + ++R +V+ + E D +EDG++WRKYGQK
Sbjct: 132 EGGEDQQEKSHTKKQLKAKKNNQKRQREA-----RVAFMTKSEVDH-LEDGYRWRKYGQK 185
Query: 135 KILHTDFPRYIY 146
+ ++ FPR Y
Sbjct: 186 AVKNSPFPRSYY 197
>At2g40740 putative WRKY-type DNA binding protein
Length = 292
Score = 42.4 bits (98), Expect = 1e-04
Identities = 25/73 (34%), Positives = 38/73 (51%), Gaps = 2/73 (2%)
Query: 76 GSDMQLTKSEDSLESNCKSTSIVKERR--GCYKRRKVSQTWEKESDRPVEDGHQWRKYGQ 133
G + LT + + ++ ST + R+ G + V+ +D P +D H WRKYGQ
Sbjct: 124 GGERNLTAAVERSGASGSSTPRQRRRKDEGEEQTVLVAALRTGNTDLPPDDNHTWRKYGQ 183
Query: 134 KKILHTDFPRYIY 146
K+IL + FPR Y
Sbjct: 184 KEILGSRFPRAYY 196
>At1g66560 putative protein
Length = 249
Score = 42.0 bits (97), Expect = 1e-04
Identities = 41/129 (31%), Positives = 61/129 (46%), Gaps = 16/129 (12%)
Query: 19 LRRGRELANQLKNILNESGDFDDINGSTTPFAEDLLKEVLTTFTNSLLFLNNNPTSEGSD 78
L RG+ AN LK +L D ST P L+ +L +F+ +L F++ P S
Sbjct: 13 LLRGQGCANSLKRLLENHKLSSD---STEP----LIYTILNSFSLALSFVD--PPS---- 59
Query: 79 MQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRP-VEDGHQWRKYGQKKIL 137
L +E SL+ N S + + + Y + + + ES P +DG WRKYGQK I
Sbjct: 60 -LLPHNESSLQ-NMTSHVLQRSSKKKYYGAEDLEYYRDESPTPRPDDGFTWRKYGQKTIK 117
Query: 138 HTDFPRYIY 146
+ + R Y
Sbjct: 118 TSPYQRCYY 126
>At4g18170 DNA binding like protein
Length = 318
Score = 41.2 bits (95), Expect = 2e-04
Identities = 26/88 (29%), Positives = 42/88 (47%), Gaps = 12/88 (13%)
Query: 71 NPTSEGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRP--------- 121
+P++ S EDS +S K + +E + K K +T K+ P
Sbjct: 108 SPSNSSSSEADHPGEDSGKSRRKRELVGEEDQISKKVGKTKKTEVKKQREPRVSFMTKSE 167
Query: 122 ---VEDGHQWRKYGQKKILHTDFPRYIY 146
+EDG++WRKYGQK + ++ +PR Y
Sbjct: 168 VDHLEDGYRWRKYGQKAVKNSPYPRSYY 195
>At3g01080 WRKY transcription factor 58 (WRKY58)
Length = 423
Score = 40.8 bits (94), Expect = 3e-04
Identities = 15/28 (53%), Positives = 20/28 (70%)
Query: 119 DRPVEDGHQWRKYGQKKILHTDFPRYIY 146
D+P +DG+ WRKYGQK I ++PR Y
Sbjct: 163 DKPADDGYNWRKYGQKPIKGCEYPRSYY 190
Score = 32.7 bits (73), Expect = 0.076
Identities = 12/25 (48%), Positives = 17/25 (68%)
Query: 122 VEDGHQWRKYGQKKILHTDFPRYIY 146
++DG++WRKYGQK + PR Y
Sbjct: 305 LDDGYRWRKYGQKVVKGNPHPRSYY 329
>At3g01970 putative WRKY-like transcriptional regulator protein
Length = 147
Score = 40.4 bits (93), Expect = 4e-04
Identities = 24/78 (30%), Positives = 40/78 (50%), Gaps = 3/78 (3%)
Query: 72 PTSEGSDMQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPVE---DGHQW 128
P S D +LT+ S +TS ++ R K+++ + ++ V+ DG++W
Sbjct: 11 PCSSSVDPRLTEFHGVDNSAQPTTSSEEKPRSKKKKKEREARYAFQTRSQVDILDDGYRW 70
Query: 129 RKYGQKKILHTDFPRYIY 146
RKYGQK + + FPR Y
Sbjct: 71 RKYGQKAVKNNPFPRSYY 88
>At1g29860 putative protein
Length = 282
Score = 40.4 bits (93), Expect = 4e-04
Identities = 25/78 (32%), Positives = 45/78 (57%), Gaps = 5/78 (6%)
Query: 73 TSEGSDMQLTKSEDSLES---NCKSTSIVKERRGCYKRRKVSQTWEKESDRP-VEDGHQW 128
T++ SD Q+ +E L + K + +++G K R+V + +S+ +EDG++W
Sbjct: 83 TNDKSD-QMEDNEGDLHGVGESSKQLTKQGKKKGEKKEREVRVAFMTKSEIDHLEDGYRW 141
Query: 129 RKYGQKKILHTDFPRYIY 146
RKYGQK + ++ +PR Y
Sbjct: 142 RKYGQKAVKNSPYPRSYY 159
>At5g56270 transcription factor NtWRKY4-like
Length = 687
Score = 40.0 bits (92), Expect = 5e-04
Identities = 32/132 (24%), Positives = 56/132 (42%), Gaps = 22/132 (16%)
Query: 33 LNESGDFDDINGSTTPFAEDLLKEVLTTFTNSLLFLNNNPTSEGSDMQLTKSEDSLESNC 92
+ S F ++ S++ F + + + + N NN +S S ++ +++ES+
Sbjct: 169 IGASFSFHPVSRSSSSFFQGTTEMMSVDYGN----YNNRSSSHQSAEEVKPGSENIESSN 224
Query: 93 KSTSIVKERRGCYKRRKVS----------QTWEKESDR--------PVEDGHQWRKYGQK 134
+ G K V+ Q E+E R P EDG+ WRKYGQK
Sbjct: 225 LYGIETDNQNGQNKTSDVTTNTSLETVDHQEEEEEQRRGDSMAGGAPAEDGYNWRKYGQK 284
Query: 135 KILHTDFPRYIY 146
+ +++PR Y
Sbjct: 285 LVKGSEYPRSYY 296
Score = 32.0 bits (71), Expect = 0.13
Identities = 12/25 (48%), Positives = 17/25 (68%)
Query: 122 VEDGHQWRKYGQKKILHTDFPRYIY 146
++DG++WRKYGQK + PR Y
Sbjct: 486 LDDGYRWRKYGQKVVKGNPNPRSYY 510
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.313 0.132 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,782,234
Number of Sequences: 26719
Number of extensions: 161871
Number of successful extensions: 484
Number of sequences better than 10.0: 102
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 379
Number of HSP's gapped (non-prelim): 117
length of query: 151
length of database: 11,318,596
effective HSP length: 90
effective length of query: 61
effective length of database: 8,913,886
effective search space: 543747046
effective search space used: 543747046
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146784.1