BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146757.7 - phase: 0
(367 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g06483 pyruvate dehydrogenase like kinase 468 e-132
At5g35750 histidine kinase (AHK2) 32 0.42
At1g50200 alanine--tRNA ligase, putative 32 0.55
At1g66340 ethylene-response protein, ETR1 32 0.72
At5g49460 ATP-citrate lyase subunit B 30 1.6
At1g69630 hypothetical protein 30 2.1
At1g22340 hypothetical protein 30 2.1
At4g21360 putative transposable element 30 2.7
At2g17820 histidine kinase 1 30 2.7
At5g63640 unknown protein (At5g63640) 29 4.7
At4g36270 putative protein 28 6.1
At3g56150 PROBABLE EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SU... 28 6.1
At4g36280 unknown protein 28 7.9
>At3g06483 pyruvate dehydrogenase like kinase
Length = 272
Score = 468 bits (1205), Expect = e-132
Identities = 227/276 (82%), Positives = 251/276 (90%), Gaps = 4/276 (1%)
Query: 92 IRSCPEVKDMKDEREFTDVIKAIKVRHNNVVPTMALGVQQLKKELKTKIDSEDLVEIHEF 151
+R+ PE+KD DE++FT +IKA+KVRHNNVVP MALGV QLKK + +S +L EIH+F
Sbjct: 1 MRAFPEIKDSGDEKDFTQMIKAVKVRHNNVVPMMALGVNQLKKGM----NSGNLDEIHQF 56
Query: 152 LDRFYLSRIGVRMLIGQHVELHNPNPPPHVVGYIHTKMSPVSVARNASEDARSICMREYG 211
LDRFYLSRIG+RMLIGQHVELHNPNPP H VGYIHTKMSP+ VARNASEDARSIC REYG
Sbjct: 57 LDRFYLSRIGIRMLIGQHVELHNPNPPLHTVGYIHTKMSPMEVARNASEDARSICFREYG 116
Query: 212 SAPEINIYGDPDFTFPYVPAHLHLMVFELVKNSLRAVQERYMDSDKVSPPIRIIVADGLE 271
SAPEINIYGDP FTFPYVP HLHLM++ELVKNSLRAVQER++DSD+V+PPIRIIVADG+E
Sbjct: 117 SAPEINIYGDPSFTFPYVPTHLHLMMYELVKNSLRAVQERFVDSDRVAPPIRIIVADGIE 176
Query: 272 DVTIKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVTTMAGYGYGLPISRL 331
DVTIK+SDEGGGI RSGLP+IFTYLYSTARNPL+E DLG+AD TMAGYGYGLPISRL
Sbjct: 177 DVTIKVSDEGGGIARSGLPRIFTYLYSTARNPLEEDVDLGIADVPVTMAGYGYGLPISRL 236
Query: 332 YARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP 367
YARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP
Sbjct: 237 YARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP 272
>At5g35750 histidine kinase (AHK2)
Length = 1176
Score = 32.3 bits (72), Expect = 0.42
Identities = 67/307 (21%), Positives = 119/307 (37%), Gaps = 71/307 (23%)
Query: 95 CPEVKDMKDEREFTDVIKA---IKVRHNNVVPTMALGVQQLKKELKTKIDSEDLVEIHEF 151
C +++++K E D+ K+ V H P + + LK + T +D++ + ++
Sbjct: 571 CQKMRELKARAEAADIAKSQFLATVSHEIRTPMNGV-LGMLKMLMDTDLDAKQM----DY 625
Query: 152 LDRFYLSRIGVRMLIGQ----------HVELHNPNPPPHVVGYIHTKMSPVSVARNASED 201
+ S + LI + +EL N P + +I +S + ++ A+E
Sbjct: 626 AQTAHGSGKDLTSLINEVLDQAKIESGRLELENV---PFDMRFILDNVSSL-LSGKANEK 681
Query: 202 ARSICMREYGSAPEINIYGDPDFTFPYVPAHLHLMVFELVKNSLRAVQER------YMDS 255
+ + P++ + GDP + ++ LV NS++ QER +
Sbjct: 682 GIELAVYVSSQVPDV-VVGDP--------SRFRQIITNLVGNSIKFTQERGHIFISVHLA 732
Query: 256 DKVSPPIRIIVADGLEDVTIKISDEGGGIPRSGLPKI--------FTYLYSTARNPLDEH 307
D+V P+ I A + + + S+ G + SG P + F YST D+
Sbjct: 733 DEVKEPLTIEDAVLKQRLALGCSESGETV--SGFPAVNAWGSWKNFKTCYSTESQNSDQI 790
Query: 308 A------DLGV----------------ADSVT--TMAGYGYGLPISRLYARYFGGDLQII 343
D GV ADS T T G G GL IS+ G++ +
Sbjct: 791 KLLVTVEDTGVGIPVDAQGRIFTPFMQADSSTSRTYGGTGIGLSISKRLVELMQGEMGFV 850
Query: 344 SMEGYGT 350
S G G+
Sbjct: 851 SEPGIGS 857
>At1g50200 alanine--tRNA ligase, putative
Length = 1027
Score = 32.0 bits (71), Expect = 0.55
Identities = 12/16 (75%), Positives = 13/16 (81%)
Query: 324 YGLPISRLYARYFGGD 339
YGLP R+YA YFGGD
Sbjct: 180 YGLPTDRIYATYFGGD 195
>At1g66340 ethylene-response protein, ETR1
Length = 738
Score = 31.6 bits (70), Expect = 0.72
Identities = 22/75 (29%), Positives = 33/75 (43%), Gaps = 13/75 (17%)
Query: 275 IKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVTTMAGYGYGLPISRLYAR 334
+K+ D G GI +PKIFT T + + + G G GL IS+ +
Sbjct: 509 VKVKDSGAGINPQDIPKIFTKFAQTQ------------SLATRSSGGSGLGLAISKRFVN 556
Query: 335 YFGGDLQIISMEGYG 349
G++ I S +G G
Sbjct: 557 LMEGNIWIES-DGLG 570
>At5g49460 ATP-citrate lyase subunit B
Length = 608
Score = 30.4 bits (67), Expect = 1.6
Identities = 27/96 (28%), Positives = 45/96 (46%), Gaps = 9/96 (9%)
Query: 238 FELVKNSLRAVQERYMDSDKVSPPIRIIVADGLEDVTIKISDEGGGIPRSGLPKIFTYLY 297
FE ++++++ E+ ++ KVSP +I ED+ I +SG + T++
Sbjct: 303 FEALESAIKETFEKLVEEGKVSPIKEVIPPQIPEDLNSAI--------KSGKVRAPTHII 354
Query: 298 STARNPL-DEHADLGVADSVTTMAGYGYGLPISRLY 332
ST + +E GV S GYG G IS L+
Sbjct: 355 STISDDRGEEPCYAGVPMSSIIEQGYGVGDVISLLW 390
>At1g69630 hypothetical protein
Length = 451
Score = 30.0 bits (66), Expect = 2.1
Identities = 16/51 (31%), Positives = 26/51 (50%), Gaps = 7/51 (13%)
Query: 79 LKVRDWYVDSFRD-------IRSCPEVKDMKDEREFTDVIKAIKVRHNNVV 122
LKV D + F D I CP ++ + ER F D I+ ++VR +++
Sbjct: 169 LKVMDLIITKFADDMGLETLITKCPVLESLTIERSFCDEIEVLRVRSQSLL 219
>At1g22340 hypothetical protein
Length = 487
Score = 30.0 bits (66), Expect = 2.1
Identities = 23/77 (29%), Positives = 33/77 (41%), Gaps = 15/77 (19%)
Query: 220 GDPDFTFPYVP-----------AHLHLMVFELVKNSLRAVQE---RYMDSDKVSPPIRII 265
G P F F +P H + + KN L +E R D D V PP+ I
Sbjct: 65 GFPSFRFESIPDGLPETDGDRTQHTPTVCMSIEKNCLAPFKEILRRINDKDDV-PPVSCI 123
Query: 266 VADGLEDVTIKISDEGG 282
V+DG+ T+ ++E G
Sbjct: 124 VSDGVMSFTLDAAEELG 140
>At4g21360 putative transposable element
Length = 1308
Score = 29.6 bits (65), Expect = 2.7
Identities = 28/115 (24%), Positives = 48/115 (41%), Gaps = 11/115 (9%)
Query: 89 FRDIRSCPE--VKDMKDEREFTDVIKAIKVRHNNVVPTMALGVQQLKKELKTKIDSEDLV 146
++DIR E VKD+ D + + ++K H T + ++++ +E DSE+ V
Sbjct: 714 YKDIRESSEQSVKDISDLEGYNEFQVSVK-EHGECSKTGGVTIEEIDQES----DSENSV 768
Query: 147 EIHEFLDRFYLSRIGVRMLIGQHVELHNPNPPPHVVGYIHTKMSPVSVARNASED 201
+ LS + E PNPP + Y H ++ V SE+
Sbjct: 769 TQEPLIASIDLSNYQS----ARDRERRAPNPPQKLADYTHFALALVMAEEIESEE 819
>At2g17820 histidine kinase 1
Length = 1207
Score = 29.6 bits (65), Expect = 2.7
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 12/81 (14%)
Query: 276 KISDEGGGIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVTTMAGYGYGLPISRLYARY 335
++ D G GI S +F E AD + T G G GL I R
Sbjct: 691 EVDDTGCGIDPSKWDSVFESF---------EQAD---PSTTRTHGGTGLGLCIVRNLVNK 738
Query: 336 FGGDLQIISMEGYGTDAYLHL 356
GG+++++ G GT L+L
Sbjct: 739 MGGEIKVVQKNGLGTLMRLYL 759
>At5g63640 unknown protein (At5g63640)
Length = 447
Score = 28.9 bits (63), Expect = 4.7
Identities = 21/55 (38%), Positives = 27/55 (48%), Gaps = 2/55 (3%)
Query: 86 VDSFRDIRSCPEVKDMKDEREFTDVIKAIKVRHNNVVPTMALGVQQLKKELKTKI 140
VD ++I C +DER+ DVIKAIK R + P L QL + L I
Sbjct: 17 VDWAKNIEICELAA--RDERQAKDVIKAIKKRLGSKNPNTQLYAVQLLEMLMNNI 69
>At4g36270 putative protein
Length = 489
Score = 28.5 bits (62), Expect = 6.1
Identities = 29/109 (26%), Positives = 46/109 (41%), Gaps = 27/109 (24%)
Query: 225 TFPYVPAHLHLMVFELVKNSLRAVQE--RYMDSDKVSPPIRIIVADGLEDVTIKISDEGG 282
T P PA + EL+ N++ +Q ++ DK++ IV D + D+GG
Sbjct: 80 TNPAAPA-----IAELIDNAVDEIQNGATFVKIDKIN-----IVKDN--SPALVFQDDGG 127
Query: 283 GIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVTTMAGYGYGLPISRL 331
G+ GL K + YS+ + S TT+ YG G S +
Sbjct: 128 GMDPDGLRKCMSLGYSSKK-------------SNTTIGQYGNGFKTSTM 163
>At3g56150 PROBABLE EUKARYOTIC TRANSLATION INITIATION FACTOR 3
SUBUNIT 8
Length = 900
Score = 28.5 bits (62), Expect = 6.1
Identities = 18/69 (26%), Positives = 30/69 (43%)
Query: 41 KNLLISAQFLQKELAIRIARRAIELESLPYGLSKKPAILKVRDWYVDSFRDIRSCPEVKD 100
K L++ FL + LA + A++ + + S K + K Y D R PEV++
Sbjct: 111 KTLVMLEDFLNEALANKEAKKKMSTSNSKALNSMKQKLKKNNKLYEDDINKYREAPEVEE 170
Query: 101 MKDEREFTD 109
K + D
Sbjct: 171 EKQPEDDDD 179
>At4g36280 unknown protein
Length = 626
Score = 28.1 bits (61), Expect = 7.9
Identities = 33/141 (23%), Positives = 56/141 (39%), Gaps = 33/141 (23%)
Query: 237 VFELVKNSLRAVQE--RYMDSDKVSPPIRIIVADGLEDVTIKISDEGGGIPRSGLPKIFT 294
+ EL+ N++ +Q ++ DK++ IV D + D+GGG+ +GL K +
Sbjct: 98 IAELLDNAVDEIQNGATFVKIDKIN-----IVKDN--SPALVFQDDGGGMDPAGLRKCMS 150
Query: 295 YLYSTARNPLDEHADLGVADSVTTMAGYGYGLPISRLYARYFGGDLQII--------SME 346
YS+ + S TT+ YG G S + G D + S +
Sbjct: 151 LGYSSKK-------------SNTTIGQYGNGFKTSTM---RLGADAIVFSRSTRGGTSTQ 194
Query: 347 GYGTDAYLHLSRLGDSQEPLP 367
G +Y L + G +P
Sbjct: 195 SVGILSYTFLRKTGQDDVTVP 215
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.320 0.139 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,400,837
Number of Sequences: 26719
Number of extensions: 370837
Number of successful extensions: 896
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 886
Number of HSP's gapped (non-prelim): 15
length of query: 367
length of database: 11,318,596
effective HSP length: 101
effective length of query: 266
effective length of database: 8,619,977
effective search space: 2292913882
effective search space used: 2292913882
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146757.7