BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146757.12 + phase: 0 /partial
(192 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g04020 unknown protein 162 1e-40
At3g49140 putative protein 38 0.003
At3g59300 unknown protein (At3g59300) 30 0.60
At4g39790 putative protein 30 0.79
At4g30130 putative protein 28 2.3
At3g21130 hypothetical protein 28 3.0
At1g78440 putative dioxygenase 28 3.0
At1g51560 unknown protein 28 3.0
At4g32500 potassium channel - protein 28 3.9
At3g61330 copia-type polyprotein 28 3.9
At3g59720 copia-type reverse transcriptase-like protein 28 3.9
At3g58150 putative protein 28 3.9
At1g48710 hypothetical protein 28 3.9
At5g54570 beta-glucosidase 27 5.1
At3g15960 hypothetical protein 27 6.7
At3g14310 pectin methylesterase like protein 27 6.7
At3g55760 unknown protein 27 8.7
At3g52670 putative protein 27 8.7
At1g21560 hypothetical protein 27 8.7
>At3g04020 unknown protein
Length = 236
Score = 162 bits (410), Expect = 1e-40
Identities = 87/192 (45%), Positives = 127/192 (65%), Gaps = 5/192 (2%)
Query: 3 ALTEKVHEVIRSEEKATRKFSYTVSGVLSSGGSS-TSRSDNLQKLL-EVTEKYSVYRFKT 60
A+ + V E I+SE KA +V +L+S SR D+L+ L+ + EKY +Y+F
Sbjct: 48 AVKKYVEEAIQSEMKAISDTPNSVRSILNSSDQMYASRCDSLRALINDAKEKYVIYKFVP 107
Query: 61 RSCTFIDGHGGTFDVNIEDLGTSKADLLAPFSAKLIDGINQSEARRRALVLLCFVYMNTN 120
SC FID +G T +++++ L SK D L +S KL+DGIN++E+RRRAL+L C +++ N
Sbjct: 108 SSCMFIDPNG-TKEIDLKVLELSKPDPLGTWSTKLVDGINKNESRRRALILFCLYFLDIN 166
Query: 121 AKDAYVTSVDRKGFDVLAKVTGPVSKDGVGQYQWKELRFMFEQEANDVETFCQHLVQMEE 180
A+DAY+ SVDRKGF +L KV P ++ +YQW+E RF FE+E DVE FC LV+ME+
Sbjct: 167 ARDAYMVSVDRKGFHLLGKV--PSEQEAGDEYQWREFRFEFEEEVKDVEAFCHQLVEMEQ 224
Query: 181 EVIKKVSASSGL 192
EV+ K + +GL
Sbjct: 225 EVVSKFTDHTGL 236
>At3g49140 putative protein
Length = 1229
Score = 38.1 bits (87), Expect = 0.003
Identities = 26/107 (24%), Positives = 50/107 (46%), Gaps = 2/107 (1%)
Query: 31 SSGGSSTSRSDNLQKLLEVTEKYSVYRFKTRSCTFIDGHGGTFDVNIEDLGTSKADLLAP 90
S G S D+ Q + + Y+ + I G +V +ED+ ++ D +A
Sbjct: 1078 SKAGGDESEIDSSQDE-KARNVVAFYKLEMIRIQLITAQGDQTEVEVEDVRKAQPDAIAH 1136
Query: 91 FSAKLIDGINQSEAR-RRALVLLCFVYMNTNAKDAYVTSVDRKGFDV 136
SA++I + +S + AL LC+ + + A++ + +D GFD+
Sbjct: 1137 ASAEIISRLEESGDKITEALKSLCWRHNSIQAEEVKLIGIDSLGFDL 1183
>At3g59300 unknown protein (At3g59300)
Length = 459
Score = 30.4 bits (67), Expect = 0.60
Identities = 24/88 (27%), Positives = 43/88 (48%), Gaps = 10/88 (11%)
Query: 54 SVYRFKTRSCTFIDGHGGTFDVNIEDLGTSKADLLAPFSAKLID-----GINQSEARRRA 108
S+YR + + +G ++++D ++ D+L ++ +I+ GIN S A
Sbjct: 323 SLYRLEIVGIELLSLYGAESSISLQDFQDAEPDILVHSTSAIIERFNNRGINSS----IA 378
Query: 109 LVLLCFVYMNTNAKDAYVTSVDRKGFDV 136
L LC +A++A + SVD G DV
Sbjct: 379 LKALC-KKKGLHAEEANLISVDSLGMDV 405
>At4g39790 putative protein
Length = 631
Score = 30.0 bits (66), Expect = 0.79
Identities = 29/101 (28%), Positives = 38/101 (36%), Gaps = 12/101 (11%)
Query: 31 SSGGSSTSRSDNLQKLLEVTEKYSVYRFKTRSCTFIDGHGGTFDVNIEDLGTSKADLLAP 90
SS SSTSR+ +Q E + S F C H +S D L
Sbjct: 309 SSSRSSTSRNPLIQTSKEDHDDESGSDFIEEFCMISGSH------------SSSLDRLYA 356
Query: 91 FSAKLIDGINQSEARRRALVLLCFVYMNTNAKDAYVTSVDR 131
+ KL D + SE R+ C N AKD S+D+
Sbjct: 357 WERKLYDEVKASEMIRKEYDRKCEQLRNQFAKDHSAKSMDK 397
>At4g30130 putative protein
Length = 725
Score = 28.5 bits (62), Expect = 2.3
Identities = 28/105 (26%), Positives = 38/105 (35%), Gaps = 17/105 (16%)
Query: 32 SGGSSTSRSDNLQKLLE-----VTEKYSVYRFKTRSCTFIDGHGGTFDVNIEDLGTSKAD 86
SGGSS S S + + +E S F SC H T D
Sbjct: 357 SGGSSRSSSSSRFLISSSGGSRASEFESSSEFSEESCMLSGSHQSTLD------------ 404
Query: 87 LLAPFSAKLIDGINQSEARRRALVLLCFVYMNTNAKDAYVTSVDR 131
L + KL D + + R A C V N + K A ++VD+
Sbjct: 405 RLYAWEKKLYDEVKSGDRIRIAYEKKCLVLRNQDVKGADSSAVDK 449
>At3g21130 hypothetical protein
Length = 367
Score = 28.1 bits (61), Expect = 3.0
Identities = 21/71 (29%), Positives = 32/71 (44%), Gaps = 9/71 (12%)
Query: 23 SYTVSGVLSSGGSSTS------RSDNLQKLLEVTEKYSVYRFKTRSCTFIDGHGGTFDVN 76
SY VS + + G +TS R D + + + +Y VY F ++S GG F
Sbjct: 144 SYKVSDIYALGYDNTSSCHKILRMDRSEDRIPIQTEYQVYDFTSKSWLVDGVAGGLF--- 200
Query: 77 IEDLGTSKADL 87
I +GT + L
Sbjct: 201 IPSIGTRRRGL 211
>At1g78440 putative dioxygenase
Length = 329
Score = 28.1 bits (61), Expect = 3.0
Identities = 15/31 (48%), Positives = 19/31 (60%), Gaps = 5/31 (16%)
Query: 120 NAKDAYVTSVDRKGFDVLAKVTGPVSKDGVG 150
NA + Y TSV + FDVL K+T DG+G
Sbjct: 125 NALEEYTTSVRKMTFDVLEKIT-----DGLG 150
>At1g51560 unknown protein
Length = 392
Score = 28.1 bits (61), Expect = 3.0
Identities = 29/109 (26%), Positives = 45/109 (40%), Gaps = 20/109 (18%)
Query: 65 FIDGHGGTFDVNIEDLGTSKADLLAPFSAKLIDGINQSEARRRALV---LLCFVYMNTNA 121
FI G G +N+ + T + D +A +DG Q+ A+ L +
Sbjct: 279 FIGGFGTVAWINVNEYETLQPDKIA------VDGGEQNLKELNAIFSKPLRELLSSEAEL 332
Query: 122 KDAYVTSVDRKGFDVLAKVTGPVSKDGVGQYQWKELRFMFEQEANDVET 170
DA + S+D KG D+ + Q+K R FE E++ VET
Sbjct: 333 DDAAIISIDSKGIDIRVRQGA----------QFKIQRLAFE-ESHGVET 370
>At4g32500 potassium channel - protein
Length = 880
Score = 27.7 bits (60), Expect = 3.9
Identities = 12/28 (42%), Positives = 18/28 (63%)
Query: 25 TVSGVLSSGGSSTSRSDNLQKLLEVTEK 52
T+SG SS G D+L++L+E+ EK
Sbjct: 818 TISGEASSSGKVVKLPDSLEELIEIGEK 845
>At3g61330 copia-type polyprotein
Length = 1352
Score = 27.7 bits (60), Expect = 3.9
Identities = 18/46 (39%), Positives = 22/46 (47%)
Query: 6 EKVHEVIRSEEKATRKFSYTVSGVLSSGGSSTSRSDNLQKLLEVTE 51
E E SEE T S T S + S T R ++Q+L EVTE
Sbjct: 777 EPTREEPPSEEPTTPPTSPTSSQIEESSSERTPRFRSIQELYEVTE 822
>At3g59720 copia-type reverse transcriptase-like protein
Length = 1272
Score = 27.7 bits (60), Expect = 3.9
Identities = 18/46 (39%), Positives = 22/46 (47%)
Query: 6 EKVHEVIRSEEKATRKFSYTVSGVLSSGGSSTSRSDNLQKLLEVTE 51
E E SEE T S T S + S T R ++Q+L EVTE
Sbjct: 777 EPTREEPPSEEPTTPPTSPTSSQIEESSSERTPRFRSIQELYEVTE 822
>At3g58150 putative protein
Length = 183
Score = 27.7 bits (60), Expect = 3.9
Identities = 24/93 (25%), Positives = 39/93 (41%), Gaps = 7/93 (7%)
Query: 95 LIDGINQSEARRRALVLLCFVYMNTNAKDAYVTSVDRKGFDVLAKVTGPVSKDGVGQYQW 154
+I +N+ A + A LL ++ T A A V V R G K+ Q +
Sbjct: 64 VIRPLNEERAVQAAADLLGELFAFTVAGAALVYEVQRNA-------RGEAKKEEKRQQEL 116
Query: 155 KELRFMFEQEANDVETFCQHLVQMEEEVIKKVS 187
E R E+ N++E Q + EE+ K ++
Sbjct: 117 AEFRRKHEKMENEIEEMKQRCCLLHEELSKPIN 149
>At1g48710 hypothetical protein
Length = 1352
Score = 27.7 bits (60), Expect = 3.9
Identities = 18/46 (39%), Positives = 22/46 (47%)
Query: 6 EKVHEVIRSEEKATRKFSYTVSGVLSSGGSSTSRSDNLQKLLEVTE 51
E E SEE T S T S + S T R ++Q+L EVTE
Sbjct: 777 EPTREEPPSEEPTTPPTSPTSSQIEESSSERTPRFRSIQELYEVTE 822
>At5g54570 beta-glucosidase
Length = 520
Score = 27.3 bits (59), Expect = 5.1
Identities = 14/30 (46%), Positives = 18/30 (59%)
Query: 32 SGGSSTSRSDNLQKLLEVTEKYSVYRFKTR 61
SG SSTS S L L E TE+ Y+F+ +
Sbjct: 491 SGSSSTSDSSKLILLEEATEQQQEYKFQEK 520
>At3g15960 hypothetical protein
Length = 474
Score = 26.9 bits (58), Expect = 6.7
Identities = 14/39 (35%), Positives = 22/39 (55%)
Query: 15 EEKATRKFSYTVSGVLSSGGSSTSRSDNLQKLLEVTEKY 53
E+ K+S VS ++ G SST S+ ++KL +KY
Sbjct: 262 EKHRKEKWSSGVSVSVTKGASSTQTSETVEKLKVTLKKY 300
>At3g14310 pectin methylesterase like protein
Length = 592
Score = 26.9 bits (58), Expect = 6.7
Identities = 15/50 (30%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Query: 2 KALTEKVHEVIRSEEKATRKFSYTVSGVLSSGG-SSTSRSDNLQKLLEVT 50
+ L+ H V+RS +TR +S V+++GG TS+ D ++ + +T
Sbjct: 49 RTLSPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLT 98
>At3g55760 unknown protein
Length = 578
Score = 26.6 bits (57), Expect = 8.7
Identities = 15/39 (38%), Positives = 20/39 (50%), Gaps = 3/39 (7%)
Query: 15 EEKATRKFS---YTVSGVLSSGGSSTSRSDNLQKLLEVT 50
+EKA K + V G GG +SD QK+LEV+
Sbjct: 78 KEKAFEKIAENVVAVDGEKEKGGDLEKKSDEFQKILEVS 116
>At3g52670 putative protein
Length = 384
Score = 26.6 bits (57), Expect = 8.7
Identities = 16/47 (34%), Positives = 24/47 (51%)
Query: 141 TGPVSKDGVGQYQWKELRFMFEQEANDVETFCQHLVQMEEEVIKKVS 187
T VSKD V +W E +++ E + +ETF E E K+V+
Sbjct: 283 TQDVSKDNVVSEKWNEPKYVPECLLSHLETFVWIRYDWEREEEKEVA 329
>At1g21560 hypothetical protein
Length = 399
Score = 26.6 bits (57), Expect = 8.7
Identities = 21/90 (23%), Positives = 42/90 (46%), Gaps = 4/90 (4%)
Query: 45 KLLEVTEKYSVYRFKTRSCTFIDGHGGTFDVNIEDLGTSKADLLAPFSAKLIDGINQSEA 104
+L E+ ++ SV+ +R DG +F N + S D F + +D + + +
Sbjct: 305 ELKELHKEASVHGVSSRHIELRDGTEFSFPTNKKK--PSYLDRYPDFKKQYLDSLREKDE 362
Query: 105 RRRALVLLCFVYMNTNA--KDAYVTSVDRK 132
R+ +L F++ TN DA+ +D++
Sbjct: 363 RKALNLLRGFIFYITNVVRDDAFKPWLDQE 392
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.131 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,922,985
Number of Sequences: 26719
Number of extensions: 153161
Number of successful extensions: 466
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 455
Number of HSP's gapped (non-prelim): 19
length of query: 192
length of database: 11,318,596
effective HSP length: 94
effective length of query: 98
effective length of database: 8,807,010
effective search space: 863086980
effective search space used: 863086980
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146757.12