BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146756.7 - phase: 0
(96 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g05840 20S proteasome subunit (PAA2) 162 3e-41
At5g35590 multicatalytic endopeptidase complex alpha subunit-like 159 3e-40
At5g66140 20S proteasome subunit PAD2 67 1e-12
At3g51260 multicatalytic endopeptidase complex alpha chain 67 1e-12
At3g14290 20S proteasome subunit PAE2 67 2e-12
At1g53850 unknown protein 67 2e-12
At3g22110 20S proteasome subunit PAC1 62 6e-11
At1g16470 20S proteasome subunit PAB1 61 8e-11
At1g79210 unknown protein 60 1e-10
At2g27020 20S proteasome subunit C8 (PAG1/PRC8_ARATH) 58 7e-10
At5g42790 20S proteasome subunit PAF1 (gb|AAC32062.1) 50 1e-07
At1g47250 20S proteasome subunit PAF2 50 1e-07
At3g23570 unknown protein 27 2.3
At5g48410 ligand-gated ion channel protein-like; glutamate recep... 26 3.9
At4g16390 salt-inducible protein homolog 26 3.9
At1g11915 unknown protein 26 3.9
At3g62300 putative protein 25 6.6
At4g21750 L1 specific homeobox gene ATML1/ovule-specific homeobo... 25 8.6
At4g04890 putative homeotic protein 25 8.6
At3g17840 receptor kinase, putative 25 8.6
>At2g05840 20S proteasome subunit (PAA2)
Length = 246
Score = 162 bits (410), Expect = 3e-41
Identities = 80/87 (91%), Positives = 83/87 (94%)
Query: 1 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKL 60
MSRGSG GYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRG DSVCVVTQKKVPDKL
Sbjct: 1 MSRGSGAGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSVCVVTQKKVPDKL 60
Query: 61 LDQTSVTHLFPITKYLGLLATGMTGVS 87
LDQ+SV+HLFP+TKYLGLLATGMT S
Sbjct: 61 LDQSSVSHLFPVTKYLGLLATGMTADS 87
>At5g35590 multicatalytic endopeptidase complex alpha subunit-like
Length = 246
Score = 159 bits (401), Expect = 3e-40
Identities = 77/84 (91%), Positives = 81/84 (95%)
Query: 1 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKL 60
MSRGSG GYDRHITIFSPEGRLFQVEYAFKAVK AGITSIGVRG DSVCVVTQKKVPDKL
Sbjct: 1 MSRGSGAGYDRHITIFSPEGRLFQVEYAFKAVKTAGITSIGVRGKDSVCVVTQKKVPDKL 60
Query: 61 LDQTSVTHLFPITKYLGLLATGMT 84
LDQ+SVTHLFPITKY+GL+ATG+T
Sbjct: 61 LDQSSVTHLFPITKYIGLVATGIT 84
>At5g66140 20S proteasome subunit PAD2
Length = 250
Score = 67.4 bits (163), Expect = 1e-12
Identities = 33/75 (44%), Positives = 47/75 (62%), Gaps = 1/75 (1%)
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YDR IT+FSP+G LFQVEYA +AV+ G ++GVRG D+V + +KK KL D S
Sbjct: 4 YDRAITVFSPDGHLFQVEYALEAVR-KGNAAVGVRGTDTVVLAVEKKSTPKLQDSRSARK 62
Query: 69 LFPITKYLGLLATGM 83
+ + ++ L G+
Sbjct: 63 IVSLDNHIALACAGL 77
>At3g51260 multicatalytic endopeptidase complex alpha chain
Length = 250
Score = 67.4 bits (163), Expect = 1e-12
Identities = 33/75 (44%), Positives = 47/75 (62%), Gaps = 1/75 (1%)
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YDR IT+FSP+G LFQVEYA +AV+ G ++GVRG D+V + +KK KL D S
Sbjct: 4 YDRAITVFSPDGHLFQVEYALEAVR-KGNAAVGVRGTDTVVLAVEKKSTPKLQDSRSARK 62
Query: 69 LFPITKYLGLLATGM 83
+ + ++ L G+
Sbjct: 63 IVSLDNHIALACAGL 77
>At3g14290 20S proteasome subunit PAE2
Length = 237
Score = 66.6 bits (161), Expect = 2e-12
Identities = 32/75 (42%), Positives = 49/75 (64%), Gaps = 1/75 (1%)
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YDR + FSPEGRLFQVEYA +A+K G T+IGV+ + V + +K++ LL+ +SV
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIK-LGSTAIGVKTKEGVVLAVEKRITSPLLEPSSVEK 66
Query: 69 LFPITKYLGLLATGM 83
+ I ++G +G+
Sbjct: 67 IMEIDDHIGCAMSGL 81
>At1g53850 unknown protein
Length = 237
Score = 66.6 bits (161), Expect = 2e-12
Identities = 32/75 (42%), Positives = 49/75 (64%), Gaps = 1/75 (1%)
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YDR + FSPEGRLFQVEYA +A+K G T+IGV+ + V + +K++ LL+ +SV
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIK-LGSTAIGVKTKEGVVLAVEKRITSPLLEPSSVEK 66
Query: 69 LFPITKYLGLLATGM 83
+ I ++G +G+
Sbjct: 67 IMEIDDHIGCAMSGL 81
>At3g22110 20S proteasome subunit PAC1
Length = 250
Score = 61.6 bits (148), Expect = 6e-11
Identities = 33/76 (43%), Positives = 47/76 (61%), Gaps = 2/76 (2%)
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLD-QTSVT 67
YD TIFSPEGRL+QVEYA +A+ AG ++IG+ D V ++ +KKV KLL TS
Sbjct: 5 YDSRTTIFSPEGRLYQVEYAMEAIGNAG-SAIGILSKDGVVLIGEKKVTSKLLQTSTSAE 63
Query: 68 HLFPITKYLGLLATGM 83
++ I ++ G+
Sbjct: 64 KMYKIDDHVACAVAGI 79
>At1g16470 20S proteasome subunit PAB1
Length = 235
Score = 61.2 bits (147), Expect = 8e-11
Identities = 29/75 (38%), Positives = 50/75 (66%), Gaps = 1/75 (1%)
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
Y +T FSP G+L Q+E+A AV +G TS+G++ ++ V + T+KK+P L+D+ SV
Sbjct: 6 YSFSLTTFSPSGKLVQIEHALTAV-GSGQTSLGIKASNGVVIATEKKLPSILVDEASVQK 64
Query: 69 LFPITKYLGLLATGM 83
+ +T +G++ +GM
Sbjct: 65 IQHLTPNIGVVYSGM 79
>At1g79210 unknown protein
Length = 235
Score = 60.5 bits (145), Expect = 1e-10
Identities = 29/75 (38%), Positives = 49/75 (64%), Gaps = 1/75 (1%)
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
Y +T FSP G+L Q+E+A AV +G TS+G++ ++ V + T+KK+P L+D+ SV
Sbjct: 6 YSFSLTTFSPSGKLVQIEHALTAV-GSGQTSLGIKASNGVVIATEKKLPSILVDEASVQK 64
Query: 69 LFPITKYLGLLATGM 83
+ +T +G + +GM
Sbjct: 65 IQHLTPNIGTVYSGM 79
>At2g27020 20S proteasome subunit C8 (PAG1/PRC8_ARATH)
Length = 249
Score = 58.2 bits (139), Expect = 7e-10
Identities = 27/78 (34%), Positives = 47/78 (59%), Gaps = 1/78 (1%)
Query: 6 GGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTS 65
G GYD +T FSP+GR+FQ+EYA KAV +G T +G++ D + + +K + K++ S
Sbjct: 5 GTGYDLSVTTFSPDGRVFQIEYAAKAVDNSG-TVVGIKCKDGIVMGVEKLIASKMMLPGS 63
Query: 66 VTHLFPITKYLGLLATGM 83
+ + ++ G+ G+
Sbjct: 64 NRRIHSVHRHAGMAVAGL 81
>At5g42790 20S proteasome subunit PAF1 (gb|AAC32062.1)
Length = 278
Score = 50.4 bits (119), Expect = 1e-07
Identities = 28/76 (36%), Positives = 42/76 (54%), Gaps = 3/76 (3%)
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YD +T +SP GRLFQVEYA +AVK G +IG+R V + K +L +
Sbjct: 6 YDTDVTTWSPTGRLFQVEYAMEAVK-QGSAAIGLRSRSHVVLACVNKAQSEL--SSHQRK 62
Query: 69 LFPITKYLGLLATGMT 84
+F + ++G+ G+T
Sbjct: 63 IFKVDDHIGVAIAGLT 78
>At1g47250 20S proteasome subunit PAF2
Length = 277
Score = 50.4 bits (119), Expect = 1e-07
Identities = 28/76 (36%), Positives = 42/76 (54%), Gaps = 3/76 (3%)
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YD +T +SP GRLFQVEYA +AVK G +IG+R V + K +L +
Sbjct: 6 YDTDVTTWSPTGRLFQVEYAMEAVK-QGSAAIGLRSRSHVVLACVNKAQSEL--SSHQRK 62
Query: 69 LFPITKYLGLLATGMT 84
+F + ++G+ G+T
Sbjct: 63 IFKVDDHIGVAIAGLT 78
>At3g23570 unknown protein
Length = 239
Score = 26.6 bits (57), Expect = 2.3
Identities = 16/44 (36%), Positives = 23/44 (51%)
Query: 30 KAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTHLFPIT 73
+A+K GITSIG G V + +KL+D T + H +T
Sbjct: 111 EALKNKGITSIGAAGFCWGAKVAVELAKEKLVDATVLLHPARVT 154
>At5g48410 ligand-gated ion channel protein-like; glutamate
receptor-like (AtGLR1.3)
Length = 860
Score = 25.8 bits (55), Expect = 3.9
Identities = 10/26 (38%), Positives = 14/26 (53%)
Query: 61 LDQTSVTHLFPITKYLGLLATGMTGV 86
L + THLFP + LGL+ G +
Sbjct: 238 LSEVIATHLFPCARRLGLMGDGFVWI 263
>At4g16390 salt-inducible protein homolog
Length = 777
Score = 25.8 bits (55), Expect = 3.9
Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 3/75 (4%)
Query: 13 ITIFSPEGRLFQVEYAFKAVKAAGI-TSIGVRGNDSVCVVTQKKVPD--KLLDQTSVTHL 69
IT+++ GR+ + E A ++ AG ++ V + C K+V D + DQ +
Sbjct: 503 ITVYACSGRVSKAEAALLQIREAGFEPTLFVLTSVIQCYGKAKQVDDVVRTFDQVLELGI 562
Query: 70 FPITKYLGLLATGMT 84
P ++ G L MT
Sbjct: 563 TPDDRFCGCLLNVMT 577
>At1g11915 unknown protein
Length = 329
Score = 25.8 bits (55), Expect = 3.9
Identities = 16/58 (27%), Positives = 24/58 (40%), Gaps = 5/58 (8%)
Query: 30 KAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTHLFPITKYLGLLATGMTGVS 87
+ K G+ R D +C+ Q V D + V H K +G +A +T VS
Sbjct: 241 ETTKGGGVCGFNTRTRDFLCLCKQGNVTTYCKDPSLVNH-----KRVGAIAGTVTAVS 293
>At3g62300 putative protein
Length = 708
Score = 25.0 bits (53), Expect = 6.6
Identities = 11/32 (34%), Positives = 17/32 (52%)
Query: 44 GNDSVCVVTQKKVPDKLLDQTSVTHLFPITKY 75
GN CV+ ++ + + SV HL P+ KY
Sbjct: 31 GNVWYCVILEENLAKSKRKKLSVRHLDPLLKY 62
>At4g21750 L1 specific homeobox gene ATML1/ovule-specific homeobox
protein A20
Length = 762
Score = 24.6 bits (52), Expect = 8.6
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 10/54 (18%)
Query: 15 IFSPEGRLFQVEYAFKAVKA--AGI--------TSIGVRGNDSVCVVTQKKVPD 58
I SPEGR ++ A + V + G+ T++ G+D V V+T+K + D
Sbjct: 498 ITSPEGRKSMLKLAERMVMSFCTGVGASTAHAWTTLSTTGSDDVRVMTRKSMDD 551
>At4g04890 putative homeotic protein
Length = 743
Score = 24.6 bits (52), Expect = 8.6
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 10/54 (18%)
Query: 15 IFSPEGRLFQVEYAFKAVKA--AGI--------TSIGVRGNDSVCVVTQKKVPD 58
I SPEGR ++ A + V + +G+ T++ G+D V V+T+K + D
Sbjct: 489 ITSPEGRKSMLKLAERMVMSFCSGVGASTAHAWTTMSTTGSDDVRVMTRKSMDD 542
>At3g17840 receptor kinase, putative
Length = 647
Score = 24.6 bits (52), Expect = 8.6
Identities = 11/27 (40%), Positives = 17/27 (62%)
Query: 17 SPEGRLFQVEYAFKAVKAAGITSIGVR 43
+PE R + EY+ AVKA + S G++
Sbjct: 320 APENRSYVNEYSPSAVKAVEVNSSGMK 346
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.139 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,086,925
Number of Sequences: 26719
Number of extensions: 71845
Number of successful extensions: 151
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 130
Number of HSP's gapped (non-prelim): 21
length of query: 96
length of database: 11,318,596
effective HSP length: 72
effective length of query: 24
effective length of database: 9,394,828
effective search space: 225475872
effective search space used: 225475872
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146756.7