BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146756.3 - phase: 0
(138 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35590 multicatalytic endopeptidase complex alpha subunit-like 248 7e-67
At2g05840 20S proteasome subunit (PAA2) 244 1e-65
At1g79210 unknown protein 90 4e-19
At1g16470 20S proteasome subunit PAB1 90 4e-19
At1g47250 20S proteasome subunit PAF2 78 1e-15
At5g42790 20S proteasome subunit PAF1 (gb|AAC32062.1) 75 8e-15
At3g51260 multicatalytic endopeptidase complex alpha chain 69 6e-13
At3g22110 20S proteasome subunit PAC1 69 6e-13
At5g66140 20S proteasome subunit PAD2 69 8e-13
At1g53850 unknown protein 55 2e-08
At3g14290 20S proteasome subunit PAE2 54 3e-08
At4g15160 cell wall protein like 48 2e-06
At2g27020 20S proteasome subunit C8 (PAG1/PRC8_ARATH) 44 3e-05
At5g40580 20S proteasome beta subunit PBB2 31 0.18
At3g27430 20S proteasome beta subunit (PBB1) 30 0.31
At5g24390 GTPase activator-like protein of Rab-like small GTPases 30 0.40
At1g73210 unknown protein 30 0.52
At1g58140 hypothetical protein 30 0.52
At1g48710 hypothetical protein 30 0.52
At3g59720 copia-type reverse transcriptase-like protein 29 0.68
>At5g35590 multicatalytic endopeptidase complex alpha subunit-like
Length = 246
Score = 248 bits (633), Expect = 7e-67
Identities = 117/138 (84%), Positives = 132/138 (94%)
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
MPVD+LAKWIADKSQVYTQHAYMRPLGVVAMV+G+D+ENGP LYKCDPAGH++GHKATSA
Sbjct: 109 MPVDILAKWIADKSQVYTQHAYMRPLGVVAMVMGVDEENGPLLYKCDPAGHFYGHKATSA 168
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFR 120
G K+QEA+NFLEKKMK +PSFT+DETVQTAI+ALQSVLQEDFKATEIEVGVV+ + PEFR
Sbjct: 169 GMKEQEAVNFLEKKMKENPSFTFDETVQTAISALQSVLQEDFKATEIEVGVVRAENPEFR 228
Query: 121 VLSTEEIDEHLTAISERD 138
L+TEEI+EHLTAISERD
Sbjct: 229 ALTTEEIEEHLTAISERD 246
>At2g05840 20S proteasome subunit (PAA2)
Length = 246
Score = 244 bits (622), Expect = 1e-65
Identities = 118/138 (85%), Positives = 128/138 (92%)
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
MP D+LAKWIADKSQVYTQHAYMRPLGVVAMVLGID+E GP LYKCDPAGH++GHKATSA
Sbjct: 109 MPADILAKWIADKSQVYTQHAYMRPLGVVAMVLGIDEERGPLLYKCDPAGHFYGHKATSA 168
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFR 120
G K+QEA+NFLEKKMK +P+FTYDETVQTAI+ALQSVLQEDFKATEIEVGVV+ D P FR
Sbjct: 169 GMKEQEAVNFLEKKMKENPAFTYDETVQTAISALQSVLQEDFKATEIEVGVVRADDPLFR 228
Query: 121 VLSTEEIDEHLTAISERD 138
L TEEIDEHLTAISERD
Sbjct: 229 SLRTEEIDEHLTAISERD 246
>At1g79210 unknown protein
Length = 235
Score = 89.7 bits (221), Expect = 4e-19
Identities = 52/134 (38%), Positives = 75/134 (55%), Gaps = 4/134 (2%)
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
+PV L + A Q +TQ +RP GV +V G DD+ GPQLY+ DP+G YF KA++
Sbjct: 105 IPVTQLVRETATVMQEFTQSGGVRPFGVSLLVAGYDDK-GPQLYQVDPSGSYFSWKASAM 163
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFR 120
G A FLEK+ D D+ + TAI L+ + + + IE+G + DK FR
Sbjct: 164 GKNVSNAKTFLEKRYTED--MELDDAIHTAILTLKEGFEGEISSKNIEIGKIGTDK-VFR 220
Query: 121 VLSTEEIDEHLTAI 134
VL+ EID++L +
Sbjct: 221 VLTPAEIDDYLAEV 234
>At1g16470 20S proteasome subunit PAB1
Length = 235
Score = 89.7 bits (221), Expect = 4e-19
Identities = 52/134 (38%), Positives = 75/134 (55%), Gaps = 4/134 (2%)
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
+PV L + A Q +TQ +RP GV +V G DD+ GPQLY+ DP+G YF KA++
Sbjct: 105 IPVTQLVRETATVMQEFTQSGGVRPFGVSLLVAGYDDK-GPQLYQVDPSGSYFSWKASAM 163
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFR 120
G A FLEK+ D D+ + TAI L+ + + + IE+G + DK FR
Sbjct: 164 GKNVSNAKTFLEKRYTED--MELDDAIHTAILTLKEGFEGEISSKNIEIGKIGADK-VFR 220
Query: 121 VLSTEEIDEHLTAI 134
VL+ EID++L +
Sbjct: 221 VLTPAEIDDYLAEV 234
>At1g47250 20S proteasome subunit PAF2
Length = 277
Score = 78.2 bits (191), Expect = 1e-15
Identities = 48/130 (36%), Positives = 78/130 (59%), Gaps = 3/130 (2%)
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
+PV L +ADK+QV TQ ++ RP GV +V G+D E+G LY P+G+YF ++A +
Sbjct: 103 LPVGRLVVHLADKAQVCTQRSWKRPYGVGLLVGGLD-ESGAHLYYNCPSGNYFEYQAFAI 161
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQ-EDFKATEIEVGVVQKDKPEF 119
GS+ Q A +LE+K ++ + ++ ++ AI A++ LQ E K++ V V+ D+P F
Sbjct: 162 GSRSQAAKTYLERKFESFQESSKEDLIKDAIMAIRETLQGETLKSSLCTVSVLGVDEP-F 220
Query: 120 RVLSTEEIDE 129
L E I +
Sbjct: 221 HFLDQESIQK 230
>At5g42790 20S proteasome subunit PAF1 (gb|AAC32062.1)
Length = 278
Score = 75.5 bits (184), Expect = 8e-15
Identities = 46/130 (35%), Positives = 78/130 (59%), Gaps = 3/130 (2%)
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
+PV L +ADK+QV TQ ++ RP GV +V G+D E+G LY P+G+YF ++A +
Sbjct: 103 LPVGRLVVHLADKAQVCTQRSWKRPYGVGLLVGGLD-ESGAHLYYNCPSGNYFEYQAFAI 161
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQ-EDFKATEIEVGVVQKDKPEF 119
GS+ Q A +LE++ ++ + ++ ++ AI A++ LQ E K++ V ++ D+P F
Sbjct: 162 GSRSQAAKTYLERRFESFGDSSREDLIKDAILAVRETLQGETLKSSLCTVAILGVDEP-F 220
Query: 120 RVLSTEEIDE 129
L E I +
Sbjct: 221 HFLDQEAIQK 230
>At3g51260 multicatalytic endopeptidase complex alpha chain
Length = 250
Score = 69.3 bits (168), Expect = 6e-13
Identities = 44/127 (34%), Positives = 65/127 (50%), Gaps = 6/127 (4%)
Query: 3 VDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENG-PQLYKCDPAGHYFGHKATSAG 61
V+ + ++IA Q YTQ +RP G+ +++G D P LY+ DP+G + KA + G
Sbjct: 105 VEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFDPYTRIPALYQTDPSGTFSAWKANATG 164
Query: 62 SKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFRV 121
FLEK K ETV+ AI AL V++ K IEV V+ +++ +
Sbjct: 165 RNSNSIREFLEKNYKESAG---QETVKLAIRALLEVVESGGK--NIEVAVMTREEGVLKQ 219
Query: 122 LSTEEID 128
L EEID
Sbjct: 220 LEEEEID 226
>At3g22110 20S proteasome subunit PAC1
Length = 250
Score = 69.3 bits (168), Expect = 6e-13
Identities = 44/135 (32%), Positives = 69/135 (50%), Gaps = 6/135 (4%)
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
MPV+ L + + D Q YTQ +RP GV + G D +G QLY DP+G+Y G KA +
Sbjct: 105 MPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKHHGFQLYMSDPSGNYGGWKAAAV 164
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATE----IEVGVVQKDK 116
G+ +Q A + L++ K+D T +E V+ A+ L + +E EV +
Sbjct: 165 GANNQAAQSILKQDYKDDA--TREEAVELALKVLTKTMDSTSLTSEKLELAEVYLTPSKT 222
Query: 117 PEFRVLSTEEIDEHL 131
++ V S E + + L
Sbjct: 223 VKYHVHSPESLTKLL 237
>At5g66140 20S proteasome subunit PAD2
Length = 250
Score = 68.9 bits (167), Expect = 8e-13
Identities = 43/127 (33%), Positives = 65/127 (50%), Gaps = 6/127 (4%)
Query: 3 VDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENG-PQLYKCDPAGHYFGHKATSAG 61
V+ + ++IA Q YTQ +RP G+ +++G D + P LY+ DP+G + KA + G
Sbjct: 105 VEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFDPYSRLPSLYQTDPSGTFSAWKANATG 164
Query: 62 SKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFRV 121
FLEK K ET++ AI AL V++ K IEV V+ +++ R
Sbjct: 165 RNSNSIREFLEKNYKESSG---QETIKLAIRALLEVVESGGK--NIEVAVMTREETGLRQ 219
Query: 122 LSTEEID 128
L EID
Sbjct: 220 LEEAEID 226
>At1g53850 unknown protein
Length = 237
Score = 54.7 bits (130), Expect = 2e-08
Identities = 31/110 (28%), Positives = 59/110 (53%), Gaps = 6/110 (5%)
Query: 19 QHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKND 78
+ + RP GV ++ G DENGP LY DP+G ++ A + GS + A + L+++ D
Sbjct: 128 EESMSRPFGVSLLIAG-HDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKD 186
Query: 79 PSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFRVLSTEEID 128
S ET+ A++ L+ V++E +++ K P + + + +E++
Sbjct: 187 LSLQEAETI--AVSILKQVMEEKVTPNNVDIA---KVAPAYHLYTPQEVE 231
>At3g14290 20S proteasome subunit PAE2
Length = 237
Score = 53.5 bits (127), Expect = 3e-08
Identities = 30/110 (27%), Positives = 59/110 (53%), Gaps = 6/110 (5%)
Query: 19 QHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKND 78
+ + RP GV ++ G DENGP LY DP+G ++ A + GS + A + L+++ D
Sbjct: 128 EESMSRPFGVSLLIAG-HDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKD 186
Query: 79 PSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFRVLSTEEID 128
+ ET+ A++ L+ V++E +++ K P + + + +E++
Sbjct: 187 ITLQEAETI--AVSILKQVMEEKVTPNNVDIA---KVAPAYHLYTPQEVE 231
>At4g15160 cell wall protein like
Length = 428
Score = 47.8 bits (112), Expect = 2e-06
Identities = 27/71 (38%), Positives = 40/71 (56%), Gaps = 2/71 (2%)
Query: 28 VVAMVLGIDDENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKNDPSFTYDETV 87
V V G D +G QLY DP+G+Y G +A + G+ +Q A + L++ K+D T +E V
Sbjct: 354 VACAVAGWDRNHGFQLYMSDPSGNYGGWQAAAVGANNQAAQSILKQDYKDDA--TREEVV 411
Query: 88 QTAIAALQSVL 98
Q AI L +
Sbjct: 412 QLAIKVLSKTM 422
>At2g27020 20S proteasome subunit C8 (PAG1/PRC8_ARATH)
Length = 249
Score = 43.5 bits (101), Expect = 3e-05
Identities = 37/135 (27%), Positives = 66/135 (48%), Gaps = 5/135 (3%)
Query: 1 MPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDENGPQLYKCDPAGHYFGHKATSA 60
+PV L++ +A + T + ++RP G ++LG D +GPQLY +P+G + + +
Sbjct: 107 VPVKELSERVASYVHLCTLYWWLRPFGC-GVILGGYDRDGPQLYMIEPSGISYRYFGAAI 165
Query: 61 GSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQSVLQE-DFKATEIEVG-VVQKDKPE 118
G Q A +EK N T E V + + E KA E+E+ + ++ K E
Sbjct: 166 GKGKQAAKTEIEK--LNLSEMTCKEGVIEVAKIIYKLHDEAKDKAFELEMSWICEESKRE 223
Query: 119 FRVLSTEEIDEHLTA 133
+ + + ++E TA
Sbjct: 224 HQKVPDDLLEEAKTA 238
>At5g40580 20S proteasome beta subunit PBB2
Length = 274
Score = 31.2 bits (69), Expect = 0.18
Identities = 25/91 (27%), Positives = 40/91 (43%), Gaps = 3/91 (3%)
Query: 30 AMVLGIDDENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKNDPSFTYDETVQT 89
A+VLG D GP L+ P G + GS A++ E K K T DE ++
Sbjct: 136 ALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKE--GLTRDEGIKL 193
Query: 90 AIAALQSVLQEDF-KATEIEVGVVQKDKPEF 119
A+ S + D + +++ V+ K E+
Sbjct: 194 VAEAICSGIFNDLGSGSNVDICVITKGHKEY 224
>At3g27430 20S proteasome beta subunit (PBB1)
Length = 273
Score = 30.4 bits (67), Expect = 0.31
Identities = 24/91 (26%), Positives = 40/91 (43%), Gaps = 3/91 (3%)
Query: 30 AMVLGIDDENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKNDPSFTYDETVQT 89
A+VLG D GP L+ P G + GS A++ E K K T DE ++
Sbjct: 136 ALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKE--GLTRDEGIKL 193
Query: 90 AIAALQSVLQEDF-KATEIEVGVVQKDKPEF 119
++ S + D + +++ V+ K E+
Sbjct: 194 VAESICSGIFNDLGSGSNVDICVITKGNKEY 224
>At5g24390 GTPase activator-like protein of Rab-like small GTPases
Length = 528
Score = 30.0 bits (66), Expect = 0.40
Identities = 15/51 (29%), Positives = 28/51 (54%)
Query: 71 LEKKMKNDPSFTYDETVQTAIAALQSVLQEDFKATEIEVGVVQKDKPEFRV 121
LE +DP Y + + ++ + SV+ +D++A VG ++K + FRV
Sbjct: 323 LEAYALHDPEIGYCQGMSDLLSPILSVIPDDYEAFWCFVGFMKKARQNFRV 373
>At1g73210 unknown protein
Length = 314
Score = 29.6 bits (65), Expect = 0.52
Identities = 19/72 (26%), Positives = 37/72 (51%), Gaps = 2/72 (2%)
Query: 58 TSAGSKDQEAINFLEKKMKNDPSFTYDETVQTAIAALQ-SVLQEDFKATEIEVGVVQKDK 116
+S G D E+ L + M ND S T+D + ++ S L +D + ++ +++D
Sbjct: 104 SSEGWSDSESEKLLSRSMSNDSSKTWDAVSEDSVFDPDGSPLLKD-RLGNLDFKYIERDP 162
Query: 117 PEFRVLSTEEID 128
P R+ T++I+
Sbjct: 163 PHKRIPLTDKIN 174
>At1g58140 hypothetical protein
Length = 1320
Score = 29.6 bits (65), Expect = 0.52
Identities = 11/42 (26%), Positives = 22/42 (52%)
Query: 37 DENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKND 78
D++ + Y C GHY + K +E N++E+K++ +
Sbjct: 275 DKSSVKCYNCGKFGHYASECKAPSNKKFEEKANYVEEKIQEE 316
>At1g48710 hypothetical protein
Length = 1352
Score = 29.6 bits (65), Expect = 0.52
Identities = 11/42 (26%), Positives = 22/42 (52%)
Query: 37 DENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKND 78
D++ + Y C GHY + K +E N++E+K++ +
Sbjct: 275 DKSSVKCYNCGKFGHYASECKAPSNKKFEEKANYVEEKIQEE 316
>At3g59720 copia-type reverse transcriptase-like protein
Length = 1272
Score = 29.3 bits (64), Expect = 0.68
Identities = 11/42 (26%), Positives = 22/42 (52%)
Query: 37 DENGPQLYKCDPAGHYFGHKATSAGSKDQEAINFLEKKMKND 78
D++ + Y C GHY + K +E N++E+K++ +
Sbjct: 275 DKSSVKCYNCGKFGHYASECKAPSNKKFKEKANYVEEKIQEE 316
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.314 0.132 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,048,820
Number of Sequences: 26719
Number of extensions: 118610
Number of successful extensions: 274
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 235
Number of HSP's gapped (non-prelim): 46
length of query: 138
length of database: 11,318,596
effective HSP length: 89
effective length of query: 49
effective length of database: 8,940,605
effective search space: 438089645
effective search space used: 438089645
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146756.3