BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146755.11 + phase: 0
(246 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52420 unknown protein 252 1e-67
At5g23920 unknown protein 157 4e-39
At1g27990 hypothetical protein 138 3e-33
At5g13000 callose synthase catalytic subunit -like protein 31 0.69
At3g60590 unknown protein 30 1.2
At4g31690 putative protein 28 3.4
At2g03290 putative Golgi-associated membrane trafficking protein 28 3.4
At3g44920 putative protein 28 4.5
At5g52070 unknown protein 28 5.9
At4g27370 myosin heavy chain - like protein 27 7.7
At4g26350 putative protein 27 7.7
At2g23430 cyclin-dependent kinase inhibitor protein 27 7.7
>At5g52420 unknown protein
Length = 242
Score = 252 bits (643), Expect = 1e-67
Identities = 130/245 (53%), Positives = 167/245 (68%), Gaps = 6/245 (2%)
Query: 1 MSGGVGPTGCDISLPKEEQEIEHKEEQDKSLNKSTSTPQRKTSFLSFRQLNCLAVVVVLS 60
MSGGVGPT DI+LPKEE+E EH + Q S ST P F SFRQLN LA+++VLS
Sbjct: 1 MSGGVGPTYNDITLPKEEEE-EH-QTQSTSTVSSTGKP---AGFFSFRQLNILAIIIVLS 55
Query: 61 ASGMVSPEDFGFVLFSVIYMCFISKVAFPSHPSKEQP-PIFTQQIKILKIYVFIGAIIGL 119
ASG+V+ +DF F + ++IY F+SK+ FP H + + P+ + KI +IYV I+GL
Sbjct: 56 ASGLVTIQDFIFTILTLIYFFFLSKLIFPPHNNPNRDAPLTSSTNKIFRIYVTAAGIVGL 115
Query: 120 YAPIAYILHGIFEGDKEGIKAATPHVFLLASQVFMEGVAFSNGFSSPIRAFVPVIYNSRR 179
PI YI GI E DK G+ AA PHVFLLASQ+FMEG+A GFS+P R VP++YN+RR
Sbjct: 116 IIPICYIFEGIVEDDKNGVSAAAPHVFLLASQIFMEGLATMFGFSAPARILVPIVYNARR 175
Query: 180 IFTIVDWLRDEISKVGEEHSGSYKRIYAGRALAVANMAFWCFNLFGFLIPVYLPRVFKAY 239
+ T+V+W+ E S+ + S +R+YAG+ LA AN+ W FNLFG LIPVYLPR FK Y
Sbjct: 176 VLTLVEWIMSEFSREDVTGTVSARRMYAGKVLAAANLGIWSFNLFGVLIPVYLPRAFKRY 235
Query: 240 YSSQK 244
Y S K
Sbjct: 236 YGSDK 240
>At5g23920 unknown protein
Length = 229
Score = 157 bits (398), Expect = 4e-39
Identities = 87/225 (38%), Positives = 129/225 (56%), Gaps = 6/225 (2%)
Query: 19 QEIEHKEEQDKSLNKSTSTPQRKTSFLSF-RQLNCLAVVVVLSASGMVSPEDFGFVLFSV 77
+EI K+ D S P K +S RQL L+ +++L+A G+V + FV+
Sbjct: 3 EEIREKDLTDSK--PSPPQPPPKRGLISRKRQLVFLSFMILLAAKGLVGIGEIAFVILCY 60
Query: 78 IYMC-FISKVAFPSHPSKEQPPIFTQQIKILKIYVFIGAIIGLYAPIAYILHGIFEGDKE 136
IY+ F+S+ AFP ++++ + + K+ + Y AIIGL P+ YI GI+ GD
Sbjct: 61 IYLYEFLSRFAFPRKQTEQKKRLSNPKNKLFQAYFLATAIIGLLFPLCYIGDGIYRGDIH 120
Query: 137 GIKAATPHVFLLASQVFMEGVAFSNGFSSPIRAFVPVIYNSRRIFTIVDWLRDEISKVGE 196
G AA PH+FLL+ Q F E + FS+ +S PI PV YN+RRIF ++DW++ E S
Sbjct: 121 GAGAAAPHLFLLSGQAFTEPIGFSDKYSMPIGILGPVFYNARRIFALLDWVKAEFSDT-- 178
Query: 197 EHSGSYKRIYAGRALAVANMAFWCFNLFGFLIPVYLPRVFKAYYS 241
+ G R+Y GR +A N W +NLFG L+PV+LPR + Y+S
Sbjct: 179 QRPGGPLRLYGGRVIASVNTVMWFYNLFGLLLPVFLPRSCEIYFS 223
>At1g27990 hypothetical protein
Length = 271
Score = 138 bits (347), Expect = 3e-33
Identities = 75/196 (38%), Positives = 115/196 (58%), Gaps = 4/196 (2%)
Query: 49 QLNCLAVVVVLSASGMVSPEDFGFVLFSVIYMCFISKVAFPSHPSKEQPPIFTQQIKILK 108
+L +A ++V SASG+V D F F+ IY+ +S++AFPSH P + K+ +
Sbjct: 47 ELQLVAFILVFSASGLVPILDMLFPAFASIYIIALSRLAFPSHGVSTASPEVFRGSKLFR 106
Query: 109 IYVFIGAIIGLYAPIAYILHGIFEGDKEGIKAATPHVFLLASQVFMEGVAFS-NGFSSPI 167
+YV G IGL+ P+AY+L G GD +++ATPH+FLL+ Q+ E V + FS P+
Sbjct: 107 LYVISGTTIGLFLPLAYVLGGFARGDDHAVRSATPHLFLLSCQILTENVISGLSLFSPPV 166
Query: 168 RAFVPVIYNSRRIFTIVDWLRDEISKVGEEHSGSYKRI---YAGRALAVANMAFWCFNLF 224
RA VP++Y RIF I+ W +D + + + + GR LA+AN+ ++ NL
Sbjct: 167 RALVPLLYTVWRIFVIIGWSKDVWFNKSLPINATPNVVTWFWFGRYLALANLGYFGVNLL 226
Query: 225 GFLIPVYLPRVFKAYY 240
FLIP +LPR F+ Y+
Sbjct: 227 CFLIPRFLPRAFEQYF 242
>At5g13000 callose synthase catalytic subunit -like protein
Length = 1963
Score = 30.8 bits (68), Expect = 0.69
Identities = 19/58 (32%), Positives = 32/58 (54%), Gaps = 8/58 (13%)
Query: 100 FTQQIKILKIYVFIGAIIGLYAPIAYILHGIFEG--DKEGIKAATPHVFLLASQVFME 155
F+ I +L +Y+F LY + +L G+ +G ++GI+ TP LASQ F++
Sbjct: 1569 FSTLITVLTVYIF------LYGRLYLVLSGLEQGLSTQKGIRDNTPLQIALASQSFVQ 1620
>At3g60590 unknown protein
Length = 329
Score = 30.0 bits (66), Expect = 1.2
Identities = 35/147 (23%), Positives = 58/147 (38%), Gaps = 34/147 (23%)
Query: 55 VVVVLSASGMVSPEDFGFVLFSVIYMCFISKVAFPSHPSKE---QPPIFTQQIKILKIYV 111
VV +LS G+ D F L + +++ A P PS+ +PP K +
Sbjct: 141 VVSLLSLIGL----DCIFNLGATLFLLMADSCARPKDPSQSCNSKPPFS------YKFWN 190
Query: 112 FIGAIIGLYAPIAYILHGIFEGDKEGIKAAT--------------PHVFLLASQVFMEGV 157
IIG P+ + G + G+ A+ P+ LLA Q E +
Sbjct: 191 MFSLIIGFLVPMLLLF-----GSQSGLLASLQPQIPFLSSAVILFPYFILLAVQTLTEIL 245
Query: 158 AFSNGFSSPIRAFVPVIYNSRRIFTIV 184
+ + SP+ PV+Y + RI ++
Sbjct: 246 TWH--WQSPVWLVTPVVYEAYRILQLM 270
>At4g31690 putative protein
Length = 461
Score = 28.5 bits (62), Expect = 3.4
Identities = 19/70 (27%), Positives = 32/70 (45%), Gaps = 4/70 (5%)
Query: 5 VGPTGCDISLPKEEQEIEHKEEQDKSLN-KSTSTPQRKTSFLSFRQLNCLAVVVV---LS 60
+GP+ C+I P+ + E +E + ++ K +K S S LNC + V +S
Sbjct: 100 LGPSCCEIQYPQSSRHEEGEESGENEISEKEGEENVQKESDKSSSDLNCFSQSVTHSNIS 159
Query: 61 ASGMVSPEDF 70
+ P DF
Sbjct: 160 RDAVSVPRDF 169
>At2g03290 putative Golgi-associated membrane trafficking protein
Length = 213
Score = 28.5 bits (62), Expect = 3.4
Identities = 19/53 (35%), Positives = 32/53 (59%), Gaps = 8/53 (15%)
Query: 12 ISLPKEEQEIEHKEEQDKSLNKSTSTPQRKTSFLSFRQLNCLAVVVVLSASGM 64
IS+ +E + +EE+ + LN+ST++ K ++LSF L VV LS +G+
Sbjct: 154 ISIHEEMYYLREREEEMQELNRSTNS---KMAWLSFGSL-----VVCLSVAGL 198
>At3g44920 putative protein
Length = 671
Score = 28.1 bits (61), Expect = 4.5
Identities = 19/64 (29%), Positives = 30/64 (46%), Gaps = 5/64 (7%)
Query: 155 EGVAFSNGFSSPIRAFVPVIYNSRRIFTIVDWLRDEISKVGEEHSGSYKRIYAGRALAVA 214
E +AF FSSPI F P+ + +V +I+ + H KR++ + A
Sbjct: 367 ETIAFLQLFSSPIHDF-PIAVTVLHLVKLVG----QINPIIVSHDKKLKRLHKNSYIHTA 421
Query: 215 NMAF 218
N+AF
Sbjct: 422 NLAF 425
>At5g52070 unknown protein
Length = 198
Score = 27.7 bits (60), Expect = 5.9
Identities = 11/24 (45%), Positives = 18/24 (74%)
Query: 173 VIYNSRRIFTIVDWLRDEISKVGE 196
V+++S ++ +VDWLRD+I GE
Sbjct: 28 VVHDSWKVGDLVDWLRDDIYWSGE 51
>At4g27370 myosin heavy chain - like protein
Length = 1126
Score = 27.3 bits (59), Expect = 7.7
Identities = 24/97 (24%), Positives = 34/97 (34%), Gaps = 8/97 (8%)
Query: 48 RQLNCLAVVVVLSASGMVSPEDFGFVLFSVIYMCFISKVAFPSHPSKEQPPIFTQ----- 102
+QL C V+ ++ S P +V Y C + P I Q
Sbjct: 738 QQLRCCGVLEIVRISRSGYPTRLTHQELAVRYGCLLLDTRISQDPLSTSKAILKQCNLPP 797
Query: 103 ---QIKILKIYVFIGAIIGLYAPIAYILHGIFEGDKE 136
Q+ KIY+ G I L Y+L GI K+
Sbjct: 798 EMYQVGYTKIYLRTGVISVLEERKKYVLRGILGLQKQ 834
>At4g26350 putative protein
Length = 431
Score = 27.3 bits (59), Expect = 7.7
Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 4/58 (6%)
Query: 154 MEGVAFSNGFSSPIRAFVPV----IYNSRRIFTIVDWLRDEISKVGEEHSGSYKRIYA 207
M G SNG ++P++ F I+N R I + DW+ I+ G H + YA
Sbjct: 221 MFGKGSSNGINAPLKYFSISNNYRIHNFRGIEKMPDWVEARIAVTGGSHKYLKEITYA 278
>At2g23430 cyclin-dependent kinase inhibitor protein
Length = 191
Score = 27.3 bits (59), Expect = 7.7
Identities = 15/43 (34%), Positives = 23/43 (52%)
Query: 8 TGCDISLPKEEQEIEHKEEQDKSLNKSTSTPQRKTSFLSFRQL 50
+ C S +++E+ H EE+DK + TST +R T F L
Sbjct: 49 SSCSGSNEYKKKELIHLEEEDKDGDTETSTYRRGTKRKLFENL 91
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.324 0.140 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,383,257
Number of Sequences: 26719
Number of extensions: 218280
Number of successful extensions: 920
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 909
Number of HSP's gapped (non-prelim): 12
length of query: 246
length of database: 11,318,596
effective HSP length: 97
effective length of query: 149
effective length of database: 8,726,853
effective search space: 1300301097
effective search space used: 1300301097
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146755.11