BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146751.9 - phase: 0
(186 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g13830 DnaJ-like protein 172 1e-43
At4g36040 DnaJ-like protein 64 5e-11
At3g13310 DnaJ protein, putative 64 6e-11
At3g62600 unknown protein 63 8e-11
At1g56300 unknown protein 63 8e-11
At2g17880 putative DnaJ protein 62 1e-10
At5g18140 unknown protein 62 2e-10
At4g28480 heat-shock protein 61 3e-10
At2g20560 putative heat shock protein 61 3e-10
At1g59980 unknown protein 61 3e-10
At5g01390 heat shock protein 40-like 61 4e-10
At1g10350 putative heat-shock protein 60 5e-10
At4g39960 DnaJ like protein 60 7e-10
At3g44110 dnaJ protein homolog atj3 60 7e-10
At4g37480 putative protein 60 9e-10
At3g47940 heat shock protein-like protein 60 9e-10
At3g08910 putative heat shock protein 60 9e-10
At2g22360 DnaJ like protein 59 1e-09
At3g17830 DnaJ, putative 59 2e-09
At1g80030 unknown protein 59 2e-09
>At4g13830 DnaJ-like protein
Length = 197
Score = 172 bits (435), Expect = 1e-43
Identities = 95/196 (48%), Positives = 134/196 (67%), Gaps = 12/196 (6%)
Query: 1 MRCY-GLTIPGSDHRLITPTLPPSLSFRPTTIPMT-----RISFGASFPKLKATFNDGMA 54
M+CY +I ++H P S +PT+IP T R F ++ + + T +D +
Sbjct: 1 MKCYKSSSILSTNHHPFFYKQQPISSLQPTSIPTTISYPTRTRFSSTRIQSRLTHDDPVK 60
Query: 55 -AEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETL 113
+E+ SFYDLLG+ ES +L +IK+AYKQLARKYHPDVSPPDRV+EYT RFI+VQEAYETL
Sbjct: 61 QSEDLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETL 120
Query: 114 SDPSRRIMYDRDMAIGIHLAFNACKCYNHSDQVSEQKGDWK----SQLAGLQRRSDSKVA 169
SDP RR++YDRD+++G +F+ + + +V E+K +WK +QL+GL+RRS+ K
Sbjct: 121 SDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQK-D 179
Query: 170 AENMSWAARVRQQRGE 185
MSWAAR+R+Q+ E
Sbjct: 180 NNTMSWAARMRRQQQE 195
>At4g36040 DnaJ-like protein
Length = 161
Score = 63.9 bits (154), Expect = 5e-11
Identities = 40/115 (34%), Positives = 59/115 (50%), Gaps = 5/115 (4%)
Query: 16 ITPTLPPSLSFR---PTTIPMTRISFGASFPKLKATFNDGMAAEERSFYDLLGIPESGSL 72
++P PPS + R P ++ P+L + S YD+L +P +
Sbjct: 20 LSPISPPSRTARISPPLVSASCSYTYTEDSPRLHQ-IPRRLTTVPASLYDVLEVPLGATS 78
Query: 73 MDIKRAYKQLARKYHPDVSPPDRVKEYT-KRFIQVQEAYETLSDPSRRIMYDRDM 126
DIK AY++LAR HPDV+ DR + F+++ AY TLSDP +R +YDR M
Sbjct: 79 QDIKSAYRRLARICHPDVAGTDRTSSSSADEFMKIHAAYCTLSDPEKRSVYDRRM 133
>At3g13310 DnaJ protein, putative
Length = 157
Score = 63.5 bits (153), Expect = 6e-11
Identities = 38/103 (36%), Positives = 56/103 (53%), Gaps = 11/103 (10%)
Query: 28 PTTIPMTRISFGASFPKLKA--TFNDGMAAEERSFYDLLGIPESGSLMDIKRAYKQLARK 85
PT P SF AS L A + + S Y+LL + E+ SL +IK AY+ LA+
Sbjct: 35 PTGAP----SFKASAQTLNAEPAVTESVRRRVSSLYELLKVNETASLTEIKTAYRSLAKV 90
Query: 86 YHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRRIMYDRDMAI 128
YHPD S D + F+++ +AY TL+DP+ R +YD + +
Sbjct: 91 YHPDASESD-----GRDFMEIHKAYATLADPTTRAIYDSTLRV 128
>At3g62600 unknown protein
Length = 346
Score = 63.2 bits (152), Expect = 8e-11
Identities = 29/67 (43%), Positives = 47/67 (69%), Gaps = 2/67 (2%)
Query: 58 RSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPS 117
+S+YD+L +P+ S IKRAY++LA KYHPD + + +E T++F ++ AYE LSD
Sbjct: 25 KSYYDVLQVPKGASDEQIKRAYRKLALKYHPDKNQGN--EEATRKFAEINNAYEVLSDEE 82
Query: 118 RRIMYDR 124
+R +Y++
Sbjct: 83 KREIYNK 89
>At1g56300 unknown protein
Length = 156
Score = 63.2 bits (152), Expect = 8e-11
Identities = 31/67 (46%), Positives = 44/67 (65%), Gaps = 2/67 (2%)
Query: 59 SFYDLLGIPESGSLMDIKRAYKQLARKYHPD--VSPPDRVKEYTKRFIQVQEAYETLSDP 116
S+Y +LGI + S+ DI+ AY++LA K+HPD P E +RF Q+QEAY L+D
Sbjct: 13 SYYTILGIRKDASVSDIRTAYRKLAMKWHPDRYARNPGVAGEAKRRFQQIQEAYSVLNDE 72
Query: 117 SRRIMYD 123
++R MYD
Sbjct: 73 NKRSMYD 79
>At2g17880 putative DnaJ protein
Length = 160
Score = 62.4 bits (150), Expect = 1e-10
Identities = 45/125 (36%), Positives = 60/125 (48%), Gaps = 19/125 (15%)
Query: 17 TPTLPPSLSFRPT---TIPMTRISFGASFPKLKATFNDGMAAEE--------------RS 59
TP L S S P+ T P +RIS P L T AE+ S
Sbjct: 11 TPFLSSSSSSSPSFSSTSPPSRIS--RISPSLSTTTASYTCAEDLPRLRQIPQRFSATAS 68
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
Y++L IP + +IK AY++LAR HPDV+ R F+++ AY TLSDP +R
Sbjct: 69 LYEILEIPVGSTSQEIKSAYRRLARICHPDVARNSRDNSSADDFMKIHAAYCTLSDPEKR 128
Query: 120 IMYDR 124
+YDR
Sbjct: 129 AVYDR 133
>At5g18140 unknown protein
Length = 333
Score = 61.6 bits (148), Expect = 2e-10
Identities = 34/102 (33%), Positives = 58/102 (56%), Gaps = 3/102 (2%)
Query: 47 ATFNDGMAAEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQV 106
A F+ ++ Y +LGI + + DIKRAY+ LARK+HPDV+ + E F V
Sbjct: 65 AAFSSSSNTGGQNHYAVLGIARNATQGDIKRAYRLLARKFHPDVNKDSKAGEL---FKSV 121
Query: 107 QEAYETLSDPSRRIMYDRDMAIGIHLAFNACKCYNHSDQVSE 148
+ +YE LS+ + R YDR + + + F+ K ++++ +V +
Sbjct: 122 RCSYEVLSNEATRTQYDRALKLQENSRFHRVKRHSYTPEVED 163
>At4g28480 heat-shock protein
Length = 348
Score = 61.2 bits (147), Expect = 3e-10
Identities = 27/65 (41%), Positives = 46/65 (70%), Gaps = 1/65 (1%)
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
+Y +L + S + D+K+AY++LA K+HPD +P ++ K+ +F Q+ EAY+ LSDP +R
Sbjct: 5 YYKVLQVDRSANDDDLKKAYRKLAMKWHPDKNPNNK-KDAEAKFKQISEAYDVLSDPQKR 63
Query: 120 IMYDR 124
+YD+
Sbjct: 64 AVYDQ 68
>At2g20560 putative heat shock protein
Length = 337
Score = 61.2 bits (147), Expect = 3e-10
Identities = 28/65 (43%), Positives = 45/65 (69%), Gaps = 1/65 (1%)
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
+Y +L + S S D+K+AY++LA K+HPD +P ++ K+ F Q+ EAYE LSDP ++
Sbjct: 5 YYKVLQVDRSASDDDLKKAYRKLAMKWHPDKNPNNK-KDAEAMFKQISEAYEVLSDPQKK 63
Query: 120 IMYDR 124
+YD+
Sbjct: 64 AVYDQ 68
>At1g59980 unknown protein
Length = 414
Score = 61.2 bits (147), Expect = 3e-10
Identities = 30/66 (45%), Positives = 44/66 (66%), Gaps = 2/66 (3%)
Query: 58 RSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPS 117
R+ Y++LGIP + + +IK AY+++A +YHPD +P D V + F +V AYE LSDP
Sbjct: 22 RNPYEVLGIPSNSTDQEIKSAYRRMALRYHPDKNPDDPVA--AEMFKEVTFAYEVLSDPE 79
Query: 118 RRIMYD 123
R +YD
Sbjct: 80 NRRLYD 85
>At5g01390 heat shock protein 40-like
Length = 335
Score = 60.8 bits (146), Expect = 4e-10
Identities = 29/74 (39%), Positives = 50/74 (67%), Gaps = 1/74 (1%)
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
FY +L + S + ++K+AY++LA K+HPD +P ++ KE +F Q+ EAY+ LSDP +R
Sbjct: 5 FYKVLEVDRSANDDELKKAYRKLAMKWHPDKNPNNK-KEAEAKFKQISEAYDVLSDPQKR 63
Query: 120 IMYDRDMAIGIHLA 133
+Y++ G++ A
Sbjct: 64 AIYEQYGEEGLNQA 77
>At1g10350 putative heat-shock protein
Length = 349
Score = 60.5 bits (145), Expect = 5e-10
Identities = 26/65 (40%), Positives = 46/65 (70%), Gaps = 1/65 (1%)
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
+Y++L + + + D+K++Y+++A K+HPD +P + KE +F Q+ EAY+ LSDP RR
Sbjct: 5 YYNVLKVNRNANEDDLKKSYRRMAMKWHPDKNPTSK-KEAEAKFKQISEAYDVLSDPQRR 63
Query: 120 IMYDR 124
+YD+
Sbjct: 64 QIYDQ 68
>At4g39960 DnaJ like protein
Length = 447
Score = 60.1 bits (144), Expect = 7e-10
Identities = 28/74 (37%), Positives = 46/74 (61%), Gaps = 3/74 (4%)
Query: 57 ERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDP 116
+ FY +LG+ ++ + +IK AY++LAR YHPDV+ ++ +F ++ AYE LSD
Sbjct: 83 DTDFYSVLGVSKNATKAEIKSAYRKLARSYHPDVNKDAGAED---KFKEISNAYEILSDD 139
Query: 117 SRRIMYDRDMAIGI 130
+R +YDR G+
Sbjct: 140 EKRSLYDRYGEAGV 153
>At3g44110 dnaJ protein homolog atj3
Length = 420
Score = 60.1 bits (144), Expect = 7e-10
Identities = 30/71 (42%), Positives = 46/71 (64%), Gaps = 8/71 (11%)
Query: 55 AEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDV-SPPDRVKEYTKRFIQVQEAYETL 113
++ FY++LG+P+S S D+K+AYK+ A K HPD P++ KE + +AYE L
Sbjct: 10 SDNTKFYEILGVPKSASPEDLKKAYKKAAIKNHPDKGGDPEKFKE-------LAQAYEVL 62
Query: 114 SDPSRRIMYDR 124
SDP +R +YD+
Sbjct: 63 SDPEKREIYDQ 73
>At4g37480 putative protein
Length = 523
Score = 59.7 bits (143), Expect = 9e-10
Identities = 25/64 (39%), Positives = 43/64 (67%)
Query: 61 YDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRRI 120
YD+L + E+ S+ +IK ++++LA++ HPD+ + ++RF+Q+ AYE LSD +R
Sbjct: 58 YDILNVSETSSIAEIKASFRRLAKETHPDLIESKKDPSNSRRFVQILAAYEILSDSEKRA 117
Query: 121 MYDR 124
YDR
Sbjct: 118 HYDR 121
>At3g47940 heat shock protein-like protein
Length = 350
Score = 59.7 bits (143), Expect = 9e-10
Identities = 26/64 (40%), Positives = 43/64 (66%)
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
+Y++L + + + D+K+AYK+LA +HPD +P R E +F ++ EAY+ LSDP +R
Sbjct: 5 YYNILKVNHNATEDDLKKAYKRLAMIWHPDKNPSTRRDEAEAKFKRISEAYDVLSDPQKR 64
Query: 120 IMYD 123
+YD
Sbjct: 65 QIYD 68
>At3g08910 putative heat shock protein
Length = 323
Score = 59.7 bits (143), Expect = 9e-10
Identities = 26/65 (40%), Positives = 45/65 (69%), Gaps = 1/65 (1%)
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
+Y +L + + D+K+AY++LA K+HPD +P ++ K+ +F Q+ EAY+ LSDP +R
Sbjct: 5 YYKVLQVDRNAKDDDLKKAYRKLAMKWHPDKNPNNK-KDAEAKFKQISEAYDVLSDPQKR 63
Query: 120 IMYDR 124
+YD+
Sbjct: 64 AIYDQ 68
>At2g22360 DnaJ like protein
Length = 442
Score = 59.3 bits (142), Expect = 1e-09
Identities = 26/68 (38%), Positives = 44/68 (64%), Gaps = 3/68 (4%)
Query: 57 ERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDP 116
+ +Y +LG+ ++ + +IK AY++LAR YHPDV+ +E +F ++ AYE LSD
Sbjct: 84 DADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSDD 140
Query: 117 SRRIMYDR 124
++ +YDR
Sbjct: 141 EKKSLYDR 148
>At3g17830 DnaJ, putative
Length = 517
Score = 58.9 bits (141), Expect = 2e-09
Identities = 40/136 (29%), Positives = 63/136 (45%), Gaps = 24/136 (17%)
Query: 16 ITPTLPPSLSFR------------PTTIPM----TRISFGASFPKLKATFNDGMAAEERS 59
+ P++ P+ SFR P T+P+ R + + T G
Sbjct: 9 LIPSINPAHSFRHQFPQPNASFYLPPTLPIFSRVRRFGISGGYRRRVITMAAGT-----D 63
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
Y L + + +L +IK +Y++LARKYHPD++ ++ +F Q+ AYE LSD +R
Sbjct: 64 HYSTLNVNRNATLQEIKSSYRKLARKYHPDMNKNPGAED---KFKQISAAYEVLSDEEKR 120
Query: 120 IMYDRDMAIGIHLAFN 135
YDR G+ FN
Sbjct: 121 SAYDRFGEAGLEGDFN 136
>At1g80030 unknown protein
Length = 500
Score = 58.9 bits (141), Expect = 2e-09
Identities = 29/76 (38%), Positives = 47/76 (61%), Gaps = 3/76 (3%)
Query: 55 AEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLS 114
A +Y LG+ +S + +IK AY++LAR+YHPDV ++ T++F ++ AYE LS
Sbjct: 71 ATSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDV---NKEPGATEKFKEISAAYEVLS 127
Query: 115 DPSRRIMYDRDMAIGI 130
D +R +YD+ G+
Sbjct: 128 DEQKRALYDQYGEAGV 143
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.134 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,269,461
Number of Sequences: 26719
Number of extensions: 169007
Number of successful extensions: 571
Number of sequences better than 10.0: 112
Number of HSP's better than 10.0 without gapping: 90
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 423
Number of HSP's gapped (non-prelim): 114
length of query: 186
length of database: 11,318,596
effective HSP length: 93
effective length of query: 93
effective length of database: 8,833,729
effective search space: 821536797
effective search space used: 821536797
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146751.9