BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146723.12 + phase: 0
(172 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g10522 unknown protein 201 2e-52
At2g28290 putative SNF2 subfamily transcription regulator 31 0.29
At3g63460 putative protein 30 0.65
At1g53110 proton pump interactor, putative 30 0.65
At1g33780 unknown protein 30 0.65
At5g02500 dnaK-type molecular chaperone hsc70.1 29 1.1
At5g42020 luminal binding protein (dbj|BAA13948.1) 29 1.4
At5g28540 luminal binding protein 29 1.4
At5g02490 dnaK-type molecular chaperone hsc70.1 - like 29 1.4
At5g03830 putative protein 28 3.2
At3g19020 hypothetical protein 28 3.2
At5g39590 unknown protein 27 7.1
At4g40010 putative serine/threonine protein kinase 27 7.1
At3g09440 heat-shock protein (At-hsc70-3) 27 7.1
At2g34680 auxin-induced protein (AIR9) 27 7.1
At5g51200 putative protein 26 9.3
At5g10500 unknown protein 26 9.3
At3g13065 LRR receptor-like protein kinase - like (SRF4), 3' par... 26 9.3
At1g70070 Similar to Synechocystis antiviral protein (At1g70070) 26 9.3
At1g35730 hypothetical protein 26 9.3
>At1g10522 unknown protein
Length = 179
Score = 201 bits (511), Expect = 2e-52
Identities = 102/157 (64%), Positives = 118/157 (74%), Gaps = 7/157 (4%)
Query: 13 IPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSIKRS--ITRAAEYKFPDPIP 70
+P SS C ++T QR KV SS+ R + RAAEYKFPDPIP
Sbjct: 26 LPIISSPAAVSCAIKSTQFFKQR-----CRTKVRDFSLSSLSRRGFVCRAAEYKFPDPIP 80
Query: 71 EFADSETEKFQNHLLNKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPGTLLVDPFID 130
EFA++ETEKF++H+LNKLSK+D+FE+SV+E+VGVCTEIF TFL SEYGGPGTLLV PFID
Sbjct: 81 EFAEAETEKFRDHMLNKLSKRDLFEDSVDEIVGVCTEIFETFLRSEYGGPGTLLVIPFID 140
Query: 131 MADIVNERGLPGGPQAARAAINWAQAHVDKDWNEWTG 167
MAD +NER LPGGPQAARAAI WAQ HVDKDW EWTG
Sbjct: 141 MADTLNERELPGGPQAARAAIKWAQDHVDKDWKEWTG 177
>At2g28290 putative SNF2 subfamily transcription regulator
Length = 1331
Score = 31.2 bits (69), Expect = 0.29
Identities = 35/132 (26%), Positives = 52/132 (38%), Gaps = 25/132 (18%)
Query: 6 WCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPS-----------INRKVSTL-PFSSI 53
W L+ FL+P+ +S S FSQ K Q S INR L PF +
Sbjct: 963 WALLNFLLPNIFNS--SEDFSQWFNKPFQSNGESSALLSEEENLLIINRLHQVLRPF--V 1018
Query: 54 KRSITRAAEYKFPDPIPEFADSETEKFQNHLLNKL---------SKKDVFEESVEEVVGV 104
R + E + P+ I E +Q L+ ++ +K SV E+ +
Sbjct: 1019 LRRLKHKVENELPEKIERLIRCEASAYQKLLMKRVEDNLGSIGNAKSRAVHNSVMELRNI 1078
Query: 105 CTEIFSTFLHSE 116
C + + LHSE
Sbjct: 1079 CNHPYLSQLHSE 1090
>At3g63460 putative protein
Length = 1097
Score = 30.0 bits (66), Expect = 0.65
Identities = 24/69 (34%), Positives = 31/69 (44%), Gaps = 2/69 (2%)
Query: 7 CLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSIKRSITRAAEYKFP 66
C+ L PS S P L S + T+ SF S NRKV + S+ T + +
Sbjct: 148 CIWDLLKPSEPSHFPLLKGSGSATQ--GEISFISWNRKVQQILASTSYNGTTVIWDLRKQ 205
Query: 67 DPIPEFADS 75
PI FADS
Sbjct: 206 KPIINFADS 214
>At1g53110 proton pump interactor, putative
Length = 439
Score = 30.0 bits (66), Expect = 0.65
Identities = 21/79 (26%), Positives = 35/79 (43%), Gaps = 5/79 (6%)
Query: 24 CFSQTTTKCIQRFSFPSINRKVSTLPFSS----IKRSITRAAEYKFPDPIPEFADSETEK 79
C K ++R F + R F +K++I AA D + E ADSE EK
Sbjct: 149 CVLNQRDKAVERIKFLRMQRDKGNAAFYQSRVVMKKAIELAASGNVRD-LEELADSEVEK 207
Query: 80 FQNHLLNKLSKKDVFEESV 98
F + N + +D +++ +
Sbjct: 208 FMSRWNNDKAFRDNYKKRI 226
>At1g33780 unknown protein
Length = 325
Score = 30.0 bits (66), Expect = 0.65
Identities = 23/65 (35%), Positives = 29/65 (44%), Gaps = 5/65 (7%)
Query: 14 PSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSIKRSITRAAEYKFPDPIPEFA 73
P SSSIP S + C F F +NRKVS P+ S+ + RA K D
Sbjct: 16 PIFSSSIPEKSSSFSRKLCELEFRF--LNRKVSASPYRSL---VVRATSKKSNDDSSSPG 70
Query: 74 DSETE 78
D+ E
Sbjct: 71 DASQE 75
>At5g02500 dnaK-type molecular chaperone hsc70.1
Length = 651
Score = 29.3 bits (64), Expect = 1.1
Identities = 14/44 (31%), Positives = 24/44 (53%), Gaps = 3/44 (6%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLH---SEYGGPGTLLVD 126
N+L++ D FE+ ++E+ +C I + E GGPG +D
Sbjct: 590 NQLAEADEFEDKMKELESICNPIIAKMYQGAGGEAGGPGASGMD 633
>At5g42020 luminal binding protein (dbj|BAA13948.1)
Length = 668
Score = 28.9 bits (63), Expect = 1.4
Identities = 12/36 (33%), Positives = 20/36 (55%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+ S+K+ ++E ++EV VC I + G PG
Sbjct: 616 NQNSEKEEYDEKLKEVEAVCNPIITAVYQRSGGAPG 651
>At5g28540 luminal binding protein
Length = 669
Score = 28.9 bits (63), Expect = 1.4
Identities = 12/36 (33%), Positives = 20/36 (55%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+ S+K+ ++E ++EV VC I + G PG
Sbjct: 616 NQNSEKEEYDEKLKEVEAVCNPIITAVYQRSGGAPG 651
>At5g02490 dnaK-type molecular chaperone hsc70.1 - like
Length = 653
Score = 28.9 bits (63), Expect = 1.4
Identities = 15/45 (33%), Positives = 23/45 (50%), Gaps = 4/45 (8%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLH----SEYGGPGTLLVD 126
N+L + D FE+ ++E+ VC I + E GGPG +D
Sbjct: 590 NQLGEADEFEDKMKELESVCNPIIAKMYQGGAGGEAGGPGASGMD 634
>At5g03830 putative protein
Length = 265
Score = 27.7 bits (60), Expect = 3.2
Identities = 25/65 (38%), Positives = 33/65 (50%), Gaps = 6/65 (9%)
Query: 39 PSINRKVST---LPFSSIKRSITRAAEYKFPDPIPEFADSETEKFQNHLLNKLSKKDVFE 95
PS R++S L FSS RS+ A+ + P+P+ S EKF N L K +K E
Sbjct: 5 PSGRRRLSKYKPLAFSSFIRSLAFASTARRKLPLPDM--SFDEKFHN-LSKKGKEKSSLE 61
Query: 96 ESVEE 100
S EE
Sbjct: 62 SSDEE 66
>At3g19020 hypothetical protein
Length = 951
Score = 27.7 bits (60), Expect = 3.2
Identities = 16/61 (26%), Positives = 27/61 (44%), Gaps = 9/61 (14%)
Query: 68 PIPEFADSETEKFQNHLLNKLSKKDV---------FEESVEEVVGVCTEIFSTFLHSEYG 118
P +F D F+ L ++ KD+ FE ++ E +G T TF H+++
Sbjct: 187 PSLKFLDIRYNDFEGKLPPEIFDKDLDAIFLNNNRFESTIPETIGKSTASVVTFAHNKFS 246
Query: 119 G 119
G
Sbjct: 247 G 247
>At5g39590 unknown protein
Length = 542
Score = 26.6 bits (57), Expect = 7.1
Identities = 18/67 (26%), Positives = 29/67 (42%), Gaps = 7/67 (10%)
Query: 15 SSSSSIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSIKRS-------ITRAAEYKFPD 67
S I SL S ++R ++ + +T+PF S+K+S IT E K D
Sbjct: 13 SEKREIESLAASTGALPLLKRSFSKLVDSQTNTVPFQSLKQSFGLSYDTITTEGEQKVSD 72
Query: 68 PIPEFAD 74
P+ +
Sbjct: 73 LFPKLLE 79
>At4g40010 putative serine/threonine protein kinase
Length = 350
Score = 26.6 bits (57), Expect = 7.1
Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
Query: 70 PEFADSETEKFQNHLLNKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPGTLLVDPFI 129
P E EK N + + +++ +SVEE+V + E ++ G G L+D I
Sbjct: 264 PLVVPPEEEKCDNGVEEEEEEEEKCRQSVEEIVKIIEEARKGVNGTDNNG-GLGLIDGSI 322
Query: 130 DMADI 134
D+ DI
Sbjct: 323 DLDDI 327
>At3g09440 heat-shock protein (At-hsc70-3)
Length = 649
Score = 26.6 bits (57), Expect = 7.1
Identities = 12/36 (33%), Positives = 22/36 (60%), Gaps = 1/36 (2%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFS-TFLHSEYGGP 120
N+L++ D FE+ ++E+ +C I + + E GGP
Sbjct: 590 NQLAECDEFEDKMKELESICNPIIAKMYQGGEAGGP 625
>At2g34680 auxin-induced protein (AIR9)
Length = 1661
Score = 26.6 bits (57), Expect = 7.1
Identities = 13/50 (26%), Positives = 25/50 (50%)
Query: 19 SIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSIKRSITRAAEYKFPDP 68
S+P L S ++ + S PS++ ++P S RS+T++ + P
Sbjct: 115 SLPELRKSSVSSLSAKTVSKPSLSESKKSVPVSPGSRSLTKSTGFSLSKP 164
>At5g51200 putative protein
Length = 1837
Score = 26.2 bits (56), Expect = 9.3
Identities = 15/59 (25%), Positives = 27/59 (45%)
Query: 35 RFSFPSINRKVSTLPFSSIKRSITRAAEYKFPDPIPEFADSETEKFQNHLLNKLSKKDV 93
R SFPS+ +S P R+ ++ E + PD +P D + L ++L ++
Sbjct: 34 RNSFPSLQNLLSFPPPKPSDRAQVQSKEIRLPDSLPISLDDQDIAISLKLSDELHLNEI 92
>At5g10500 unknown protein
Length = 848
Score = 26.2 bits (56), Expect = 9.3
Identities = 14/34 (41%), Positives = 17/34 (49%), Gaps = 5/34 (14%)
Query: 65 FPDPIPEFA-----DSETEKFQNHLLNKLSKKDV 93
FPD +PEFA D + H NK S K+V
Sbjct: 101 FPDQVPEFAMNEDDDDDAPVSPRHHKNKTSNKNV 134
>At3g13065 LRR receptor-like protein kinase - like (SRF4), 3'
partial
Length = 643
Score = 26.2 bits (56), Expect = 9.3
Identities = 30/132 (22%), Positives = 58/132 (43%), Gaps = 14/132 (10%)
Query: 3 SSTWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSI--KRSITRA 60
S T+ L+P +++ S T+ ++ FS + S + + + +I R
Sbjct: 370 SPTFHLIPSTQVAATPDRSSTSQDSPDTRGVKAFSLADLQNTASCFSPNRLLGEGTIGRV 429
Query: 61 AEYKFPD----PIPEFADS-----ETEKFQNHLLNKLSKKDVFEESVEEVVGVCTEIFST 111
+ KF D + E S E+F +H+++ +S + +++ E+VG C+E
Sbjct: 430 YKAKFQDGRKFAVKEIDSSLLGKGNPEEF-SHIVSSISS--IHHKNMAELVGYCSEQGRN 486
Query: 112 FLHSEYGGPGTL 123
L EY G+L
Sbjct: 487 MLVYEYFTSGSL 498
>At1g70070 Similar to Synechocystis antiviral protein (At1g70070)
Length = 1171
Score = 26.2 bits (56), Expect = 9.3
Identities = 20/86 (23%), Positives = 39/86 (45%), Gaps = 3/86 (3%)
Query: 26 SQTTTKCIQRFSFPSINRKVSTLP-FSSIKRSITRAAEYKFPDPIPEFADSETEKFQNHL 84
S T + + F FPS++R +S P FS K I F + + S+++ ++
Sbjct: 8 SLTASSSFKFFHFPSLHRSLSHSPNFSFTKSLILNPNHLSFKSTLNSLSPSQSQLYEEED 67
Query: 85 LNKLSKKDV--FEESVEEVVGVCTEI 108
+ ++D +E+ +E + EI
Sbjct: 68 DEEEEEEDEDDDDEAADEYDNISDEI 93
>At1g35730 hypothetical protein
Length = 564
Score = 26.2 bits (56), Expect = 9.3
Identities = 15/48 (31%), Positives = 25/48 (51%), Gaps = 4/48 (8%)
Query: 72 FADSETEKFQNHLLNKLSKKDVFEESVEEVVG--VCTEIFSTFLHSEY 117
+A+ T+KF +H++ K +K + ES E+V +C F L Y
Sbjct: 472 YAELATQKFSSHVIEKCLRK--YPESRAEIVRELLCVPNFEYLLQDPY 517
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.132 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,252,718
Number of Sequences: 26719
Number of extensions: 175540
Number of successful extensions: 504
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 497
Number of HSP's gapped (non-prelim): 21
length of query: 172
length of database: 11,318,596
effective HSP length: 92
effective length of query: 80
effective length of database: 8,860,448
effective search space: 708835840
effective search space used: 708835840
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146723.12