BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146722.13 + phase: 0
(142 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g32591 unknown protein 175 8e-45
At3g16250 putative 2Fe-2S iron-sulfur cluster protein 91 2e-19
At3g05470 unknown protein 33 0.051
At5g17980 phosphoribosylanthranilate transferase-like protein 33 0.067
At2g43710 stearoyl-ACP desaturase 30 0.56
At2g19710 hypothetical protein 29 0.74
At1g67420 29 0.96
At3g22070 unknown protein 28 1.6
At2g38910 putative calcium-dependent protein kinase 28 1.6
At1g14280 unknown protein 28 2.1
At5g23750 Unknown protein (MRO11.3) 27 2.8
At3g14480 hypothetical protein 27 2.8
At5g14830 putative protein 27 3.7
At2g46780 putative RNA-binding protein 27 3.7
At1g70140 hypothetical protein 27 3.7
At5g20655 24 kDa vacuolar protein - like 27 4.8
At2g22300 Calmodulin-binding transcription activator 3 (CAMTA3) 27 4.8
At1g79480 hypothetical protein 27 4.8
At1g63550 predicted GPI-anchored protein 27 4.8
At5g45840 receptor protein kinase-like protein 26 6.2
>At4g32591 unknown protein
Length = 173
Score = 175 bits (443), Expect = 8e-45
Identities = 89/130 (68%), Positives = 97/130 (74%), Gaps = 23/130 (17%)
Query: 36 ANSPESPSP--PPEIELEFVGPKPEGDGSFPVDKVKAISGEKLMRSIMSDNKIDLYATYG 93
A+SP+ S P EIELEF GPKP DGS+PVDK KA+SG+KL+RSIM DNKI+LYA YG
Sbjct: 44 ASSPQGISAVTPLEIELEFFGPKPGSDGSYPVDKAKAVSGDKLLRSIMQDNKIELYAAYG 103
Query: 94 KLMNCGGGGSCGTCIVE---------------------KPESWRLACQTIVGNKENSGKV 132
K+MNCGGGGSCGTCIVE KPESWRLACQTIVGNKENSGKV
Sbjct: 104 KVMNCGGGGSCGTCIVEILDGRDLLNERTDTENRYLKKKPESWRLACQTIVGNKENSGKV 163
Query: 133 VVQRIPQWKK 142
VVQRIPQWKK
Sbjct: 164 VVQRIPQWKK 173
>At3g16250 putative 2Fe-2S iron-sulfur cluster protein
Length = 204
Score = 90.9 bits (224), Expect = 2e-19
Identities = 58/156 (37%), Positives = 81/156 (51%), Gaps = 35/156 (22%)
Query: 20 PTIYFPRRRPNS-----VCLVANSPES-------PSPPPEIELEFVGPKPEGDGSFPVDK 67
P YF R + N+ V ++ +P S P PP ++ FV DG+ V
Sbjct: 30 PNSYFFRSKHNAARTKTVRAISTAPASQPPAADEPDEPPAVDFAFVHSVLLPDGTPDVHW 89
Query: 68 VKAISGEKLMRSIMSDNKIDLYATYGK-LMNCGGGGSCGTCIVE---------------- 110
+A G+KL R IM D+ I+LY Y K L NC G G+C TC+VE
Sbjct: 90 RRANGGQKL-RDIMLDSNIELYGPYSKPLSNCAGVGTCATCMVEIVNGKELLNPRTDIEK 148
Query: 111 -----KPESWRLACQTIVGNKENSGKVVVQRIPQWK 141
KP++WRLACQT VGN +++G VV+Q++P+WK
Sbjct: 149 EKLKRKPKNWRLACQTNVGNPDSTGLVVIQQLPEWK 184
>At3g05470 unknown protein
Length = 884
Score = 33.1 bits (74), Expect = 0.051
Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 3/74 (4%)
Query: 3 CMNNIFTPFPLTAGNRNPTIYFPRRRPNSVCLVANSPESPSPPPEIELEFVGPKPEGDGS 62
C ++ T FP+ N P P P + + + +PPP + L+F +P G
Sbjct: 409 CASSSKTSFPINVPNSQPRPPPPPPPPQQLQVAGINK---TPPPPLSLDFSERRPLGKDG 465
Query: 63 FPVDKVKAISGEKL 76
P+ K+K + +K+
Sbjct: 466 APLPKLKPLHWDKV 479
>At5g17980 phosphoribosylanthranilate transferase-like protein
Length = 1049
Score = 32.7 bits (73), Expect = 0.067
Identities = 16/53 (30%), Positives = 26/53 (48%)
Query: 35 VANSPESPSPPPEIELEFVGPKPEGDGSFPVDKVKAISGEKLMRSIMSDNKID 87
V + + P PPPE GPKP+ + S P+ + + GE+ S N+ +
Sbjct: 181 VKETVKPPQPPPEESSPAEGPKPDEEASPPLQENATVGGEEPPASESDKNEAE 233
>At2g43710 stearoyl-ACP desaturase
Length = 401
Score = 29.6 bits (65), Expect = 0.56
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Query: 10 PFPLTAGNRNPTIYFPRRRPNSVCLVANSPESPSPPPEIE 49
P+ + R PT F R P +CL ++SP S P E+E
Sbjct: 13 PYKFPSSTRPPTPSF--RSPKFLCLASSSPALSSGPKEVE 50
>At2g19710 hypothetical protein
Length = 918
Score = 29.3 bits (64), Expect = 0.74
Identities = 14/36 (38%), Positives = 19/36 (51%), Gaps = 2/36 (5%)
Query: 35 VANSPESP--SPPPEIELEFVGPKPEGDGSFPVDKV 68
V+ SP + P P + + GP E DG P+DKV
Sbjct: 578 VSTSPPASYHEPDPHAKFDNYGPNSESDGDQPIDKV 613
>At1g67420
Length = 876
Score = 28.9 bits (63), Expect = 0.96
Identities = 12/34 (35%), Positives = 19/34 (55%)
Query: 77 MRSIMSDNKIDLYATYGKLMNCGGGGSCGTCIVE 110
M S+ +D + + A Y +N G G CG+C+ E
Sbjct: 139 MNSLDTDASVLMNAHYDSPVNSPGAGDCGSCVGE 172
>At3g22070 unknown protein
Length = 146
Score = 28.1 bits (61), Expect = 1.6
Identities = 19/56 (33%), Positives = 22/56 (38%), Gaps = 7/56 (12%)
Query: 9 TPFPLTAGNRNPTIYFPRRRPNSVCLVANSPESPSPPPEIELEFVGPKPEGDGSFP 64
TPFP N NP P PN PE PPP+++ P P D P
Sbjct: 65 TPFPNPNPNPNPNPP-PPSTPNP------PPEFSPPPPDLDTTTAPPPPSTDIPIP 113
>At2g38910 putative calcium-dependent protein kinase
Length = 583
Score = 28.1 bits (61), Expect = 1.6
Identities = 31/103 (30%), Positives = 47/103 (45%), Gaps = 18/103 (17%)
Query: 38 SPESPSPPPEIELEFVGPKP-----EGDGSFPVDK---VKAISGEKL-MRSIM---SDNK 85
+P +P PP ++ E PKP E S P K +K ++ L + S++ ++N
Sbjct: 71 TPSTPPPPVKMANEEPPPKPITENKEDPNSKPQKKEAHMKRMASAGLQIDSVLGRKTENL 130
Query: 86 IDLYATYGKLMNCGGGGSCGTCI--VEKPESWRLACQTIVGNK 126
D+Y+ KL G G GT V+K AC+TI K
Sbjct: 131 KDIYSVGRKL----GQGQFGTTFLCVDKKTGKEFACKTIAKRK 169
>At1g14280 unknown protein
Length = 442
Score = 27.7 bits (60), Expect = 2.1
Identities = 20/76 (26%), Positives = 30/76 (39%), Gaps = 8/76 (10%)
Query: 41 SPSPPPEIELE--FVGPKPEGDGSFPVDKVKAISGEKLMRSIMSDNKIDLYATYGKLMNC 98
SP P PE+ +E FVGPK S +++ S ++ ++ K N
Sbjct: 98 SPLPDPEVPIERIFVGPKQSSKNSSETPSLRSESSWNSQSLLLQSKYVE------KKKNI 151
Query: 99 GGGGSCGTCIVEKPES 114
SC + EK S
Sbjct: 152 KKNSSCNSYFQEKDMS 167
>At5g23750 Unknown protein (MRO11.3)
Length = 202
Score = 27.3 bits (59), Expect = 2.8
Identities = 28/123 (22%), Positives = 46/123 (36%), Gaps = 4/123 (3%)
Query: 14 TAGNRNPTIYFPRRRPNSVCLVANSPESPSPPPEIELEFVGPKPEGDGSFPVDKV-KAIS 72
T PT P +P + VA + +PPP + + + D V V K +
Sbjct: 12 TVSEPTPTPEVPVEKPAAAADVAPQEKPVAPPPVLPSPAPAEEKQEDSKAIVPVVPKEVE 71
Query: 73 GEKLMRSIMSDNKIDLYATYGKLMNCGGGGSCGTCIVEKPESWRLACQTIVGNKENSGKV 132
EK S+ D + T ++ C VE +L+ +G+ EN+ K
Sbjct: 72 EEKKEGSVNRDAVLARVETEKRMSLIKAWEEAEKCKVENKAEKKLSS---IGSWENNKKA 128
Query: 133 VVQ 135
V+
Sbjct: 129 AVE 131
>At3g14480 hypothetical protein
Length = 175
Score = 27.3 bits (59), Expect = 2.8
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 15/76 (19%)
Query: 34 LVANSPESPSPPPEIELEFVGPKPEGDGSFPVDKVKAISGEKLMRSIMSDNKIDLYA--- 90
++ + + PSPPP L P+P V + K++S K R ++ L A
Sbjct: 85 IITSDTKLPSPPPPSPLP---PRP-------VQRPKSVSRTK-DRELIDTGTAMLVASSL 133
Query: 91 -TYGKLMNCGGGGSCG 105
T G +C GGGS G
Sbjct: 134 LTSGSASSCSGGGSHG 149
>At5g14830 putative protein
Length = 757
Score = 26.9 bits (58), Expect = 3.7
Identities = 23/76 (30%), Positives = 33/76 (43%), Gaps = 15/76 (19%)
Query: 12 PLTAGNRNPTIYFPRRRPNSVCLVANSPES-------PSPPPEIELEFVGPKPEGDGSFP 64
P T ++NP RP S+ L P P+PP + LEFVG E P
Sbjct: 561 PDTVADKNP-------RPGSLPLANPEPNQRPAEGLLPTPPAQQRLEFVGLTTETQFK-P 612
Query: 65 VDKVKAISGEKLMRSI 80
+D + +KL ++I
Sbjct: 613 IDLPEYEETQKLEQAI 628
>At2g46780 putative RNA-binding protein
Length = 304
Score = 26.9 bits (58), Expect = 3.7
Identities = 18/55 (32%), Positives = 21/55 (37%), Gaps = 9/55 (16%)
Query: 17 NRNPTIYFPRRRPNSVCLVANSPESPSPP---------PEIELEFVGPKPEGDGS 62
N NP I R N CL A P P+ P P + V P P+ GS
Sbjct: 82 NMNPVIDGRRANCNLACLGAQKPRPPTSPRHGTGRFRSPGSGVGLVAPSPQFRGS 136
>At1g70140 hypothetical protein
Length = 760
Score = 26.9 bits (58), Expect = 3.7
Identities = 18/48 (37%), Positives = 25/48 (51%), Gaps = 4/48 (8%)
Query: 25 PRRRPNSVCLVANSPESPSPPPEIELEFVGPKPEGDGSFPVDKVKAIS 72
P P S+ + ++P +PSPPP I+ G P PV KV A+S
Sbjct: 238 PPPPPPSIAVKQSAP-TPSPPPPIK---KGSSPSPPPPPPVKKVGALS 281
>At5g20655 24 kDa vacuolar protein - like
Length = 616
Score = 26.6 bits (57), Expect = 4.8
Identities = 14/46 (30%), Positives = 21/46 (45%)
Query: 83 DNKIDLYATYGKLMNCGGGGSCGTCIVEKPESWRLACQTIVGNKEN 128
DN I + + + GG G C +C+ E R A Q+ G K +
Sbjct: 176 DNAILVSSHIDTVFTTGGAGDCSSCVAVMLELARSASQSAHGFKNS 221
>At2g22300 Calmodulin-binding transcription activator 3 (CAMTA3)
Length = 1042
Score = 26.6 bits (57), Expect = 4.8
Identities = 15/45 (33%), Positives = 19/45 (41%)
Query: 57 PEGDGSFPVDKVKAISGEKLMRSIMSDNKIDLYATYGKLMNCGGG 101
P D S VDK K I+ + + + ID L NCG G
Sbjct: 266 PVTDSSIMVDKSKTINSPGVTNGLKNRKSIDSQTWEEILGNCGSG 310
>At1g79480 hypothetical protein
Length = 356
Score = 26.6 bits (57), Expect = 4.8
Identities = 19/58 (32%), Positives = 23/58 (38%), Gaps = 5/58 (8%)
Query: 12 PLTAGNRNP--TIYFPRRRPNSVCLVANSPES---PSPPPEIELEFVGPKPEGDGSFP 64
P ++ N NP + P PN V N PES P+PP P P S P
Sbjct: 103 PESSSNPNPPDSSSNPNSNPNPPVTVPNPPESSSNPNPPDSSSNPNSNPNPPESSSNP 160
>At1g63550 predicted GPI-anchored protein
Length = 323
Score = 26.6 bits (57), Expect = 4.8
Identities = 18/54 (33%), Positives = 25/54 (45%)
Query: 19 NPTIYFPRRRPNSVCLVANSPESPSPPPEIELEFVGPKPEGDGSFPVDKVKAIS 72
+P+ PR P ++ P++PSPP P P G SF V K + IS
Sbjct: 254 SPSAPPPRSPPPKSSPPSSLPQTPSPPLVFTPPQNVPNPSGSFSFNVLKGQHIS 307
>At5g45840 receptor protein kinase-like protein
Length = 692
Score = 26.2 bits (56), Expect = 6.2
Identities = 19/49 (38%), Positives = 24/49 (48%), Gaps = 6/49 (12%)
Query: 19 NPTIYFPRRR---PNSVCLVANSPESPSPPPEIELEFVGPKPEGDGSFP 64
N T F RR S +P++PSP PEI + V P+ GSFP
Sbjct: 236 NETSIFKRRELLEETSNLAAMPAPDTPSPSPEI-ITIVFPR--SSGSFP 281
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.137 0.430
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,117,171
Number of Sequences: 26719
Number of extensions: 198368
Number of successful extensions: 680
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 662
Number of HSP's gapped (non-prelim): 36
length of query: 142
length of database: 11,318,596
effective HSP length: 89
effective length of query: 53
effective length of database: 8,940,605
effective search space: 473852065
effective search space used: 473852065
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146722.13