BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146683.17 - phase: 0
(494 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g59780 putative protein 164 8e-41
At2g30930 unknown protein 33 0.47
At5g39390 receptor protein kinase -like protein 32 0.62
At3g62040 putative protein 32 1.0
At5g55860 myosin heavy chain-like 31 1.4
At3g28790 unknown protein 31 1.4
At5g59730 unknown protein 31 1.8
At4g33500 unknown protein 31 1.8
At3g53040 late embryogenesis abundant protein - like 31 1.8
At2g34680 auxin-induced protein (AIR9) 31 1.8
At2g30740 putative protein kinase 31 1.8
At5g16680 putative protein 30 4.0
At3g61450 syntaxin of plants 73 (SYP73) 30 4.0
At2g43230 protein kinase like protein 30 4.0
At2g41450 hypothetical protein 30 4.0
At5g57610 putative protein 29 6.8
At5g44560 unknown protein 29 6.8
At2g36500 unknown protein (At2g36500) 29 6.8
>At3g59780 putative protein
Length = 663
Score = 164 bits (416), Expect = 8e-41
Identities = 87/218 (39%), Positives = 142/218 (64%), Gaps = 12/218 (5%)
Query: 80 PEAILSSTDITSGKIESVPSLIKGGNESLAATKASATEVFAGINETFSDSINKGENALRS 139
P+A S D +SG +S S SL KAS + +G+ E+FS S+N+GENA+++
Sbjct: 82 PDAKASFDDFSSGLKQSFSS-------SLPDAKASVDDFSSGVKESFSSSLNQGENAVKN 134
Query: 140 SVDTATSFIDSVVKNATTSADNAFSKVFSAADQTGDLANKKITSFSSEIDGVTSKAPGLV 199
++++ +S + S+ KNA+ D+A ++ FS DQTGD+A K +SFS+ + +++A +
Sbjct: 135 TLESFSSSVTSITKNASEVVDSAVNRAFSTLDQTGDVAGDKFSSFSTGLKEASNRAAVIA 194
Query: 200 IDVLRRTVVAVESSLSSGASYVVYLYGSAKEFIPAEIRDTVNIYEDKAAQVLRPVGSATQ 259
ID+LR++V E S+++G S+VVY YGSAKE +P +++ +N ED A +VL PVG+ Q
Sbjct: 195 IDLLRQSVSLGERSVTNGVSFVVYSYGSAKELLPPDVKSALNSSEDVALKVLSPVGAVLQ 254
Query: 260 -----QIYMAFYSLEKSLGLDPNDPIIPFVVFVGSSAT 292
Q+ +A LE+++GLDP+DPI+ +FVG++ T
Sbjct: 255 QYHDEQVSVAIGGLERNIGLDPDDPILHLFLFVGTTGT 292
>At2g30930 unknown protein
Length = 164
Score = 32.7 bits (73), Expect = 0.47
Identities = 29/116 (25%), Positives = 47/116 (40%), Gaps = 12/116 (10%)
Query: 94 IESVPSLIKGGNESLAATK----ASATEVFAGINETFSDSINKGENALRSSVDT------ 143
+E S + ES+ +T AS T+ +N+T ++ E + +SV+T
Sbjct: 5 LEKATSALSEAKESVTSTAESVTASLTDAEKTVNQTARSTLTDAETTVAASVETVKTEAA 64
Query: 144 -ATSFIDSVVKNATTSADNAFSKVFSAADQTGDLANKKITSFSSE-IDGVTSKAPG 197
A V A + D AFS+ A K + SS+ + GVT+ G
Sbjct: 65 AAPDKASGVSTQAKDAVDKAFSRGIEGAKSLLQTFEAKSSDISSKLVGGVTNLVSG 120
>At5g39390 receptor protein kinase -like protein
Length = 502
Score = 32.3 bits (72), Expect = 0.62
Identities = 28/94 (29%), Positives = 44/94 (46%), Gaps = 7/94 (7%)
Query: 49 RYSLSTVPEELVDFAKQSTEVSEPLVAPTVHPEAILSSTDITSG-KIESVPSLIKGGNES 107
R+S T + L++F Q TE ++A H + IT G K E V SL GG +
Sbjct: 26 RFSNETDMQALLEFKSQVTENKREVLASWNHSFPLCHWIGITCGRKQERVTSLDLGGFKL 85
Query: 108 LAATKASATEVFAGINETFSDSINKGENALRSSV 141
+ S N +F S+N G+N+ +S++
Sbjct: 86 SGSISPSIG------NLSFLRSLNLGDNSFQSNI 113
>At3g62040 putative protein
Length = 249
Score = 31.6 bits (70), Expect = 1.0
Identities = 28/92 (30%), Positives = 42/92 (45%), Gaps = 15/92 (16%)
Query: 165 KVFSAADQTGDLAN-KKITSFSSEIDGVTS-KAPGLVIDVLRRTVVAVESSLSSGASYVV 222
+ F AA + D+ + +K F I + S KA GL +TV ES L GA Y +
Sbjct: 159 EAFEAAIRIADIVDPRKTIFFDDSIRNIASAKATGL------KTVFVGESVLVPGADYAL 212
Query: 223 YLYGSAKEFIPAEIRDTVNIYEDKAAQVLRPV 254
+ KE IP +++ED + L P+
Sbjct: 213 SSIHNIKEAIP-------DLWEDNKDEKLEPI 237
>At5g55860 myosin heavy chain-like
Length = 649
Score = 31.2 bits (69), Expect = 1.4
Identities = 34/153 (22%), Positives = 59/153 (38%), Gaps = 14/153 (9%)
Query: 46 GEFRYSLSTVPEELVDFAKQSTEVSEPLVAPTVHPEAILSSTDITSGKIESVPSLIKGGN 105
GE L T +EL + S E+ E E + + S KIE + I N
Sbjct: 156 GEVCKELDTAKQELRKIRQVSNEILETKTVALSKVEEAKKVSKVHSEKIELLRKEIAAVN 215
Query: 106 ESLAATKASATEVFAGINETFSD------SINKG-ENALRSSVDTATSFIDSVVKNATTS 158
ES+ TK + ++ +E F++ S G E + + S+ F K
Sbjct: 216 ESVEQTKLACSQARKEQSEIFAEKEIQQKSYKAGMEESAKKSLALKNEFDPEFAKKLEVQ 275
Query: 159 ADNAFSKVFSAADQTGDLANKKITSFSSEIDGV 191
++++ +L + T+ +S+ID V
Sbjct: 276 LTETYNEI-------DELQKQMETAKASDIDSV 301
>At3g28790 unknown protein
Length = 608
Score = 31.2 bits (69), Expect = 1.4
Identities = 41/165 (24%), Positives = 67/165 (39%), Gaps = 12/165 (7%)
Query: 39 DNSSLSTGEFRYSLSTVPEELVDFAKQSTEVSEPLVAPTVHPEAILSSTDITSGKIESVP 98
++ S ++G + T D K +T S +P+ P + + T GK S
Sbjct: 340 ESESAASGSVSKTKETNKGSSGDTYKDTTGTSSG--SPSGSPSGSPTPSTSTDGKASS-- 395
Query: 99 SLIKGGNESLAATKASATEVFAGINETFSDSINKGENALRSSVDTATSFIDSVVKNATTS 158
KG + A ASA+ + E + S K N+ SS ++T+ + V+ T+S
Sbjct: 396 ---KGSASASAGASASASAGASASAEESAASQKKESNSKSSSSSSSTTSVKE-VETQTSS 451
Query: 159 ADNAF----SKVFSAADQTGDLANKKITSFSSEIDGVTSKAPGLV 199
N+F K ++ + K TS S+ TS A LV
Sbjct: 452 EVNSFISNLEKKYTGNSELKVFFEKLKTSMSASAKLSTSNAKELV 496
>At5g59730 unknown protein
Length = 634
Score = 30.8 bits (68), Expect = 1.8
Identities = 24/87 (27%), Positives = 41/87 (46%), Gaps = 2/87 (2%)
Query: 158 SADNAFSKVFSAADQTGDLANKKITSFSSEIDGVTSKAP--GLVIDVLRRTVVAVESSLS 215
SA S+V ++ + GD +T F S I TSK P G + L R V+ S L+
Sbjct: 318 SAAAVRSQVINSLARLGDATRLMMTDFESAIQKETSKTPIIGGGVHPLTRYVMNYLSFLA 377
Query: 216 SGASYVVYLYGSAKEFIPAEIRDTVNI 242
+ + ++ + K +P + D++ I
Sbjct: 378 DYSDSIAAIFENWKLSVPTPLPDSLYI 404
>At4g33500 unknown protein
Length = 724
Score = 30.8 bits (68), Expect = 1.8
Identities = 30/111 (27%), Positives = 51/111 (45%), Gaps = 8/111 (7%)
Query: 22 SLRSSFTAQAIKTVSAWDNSSLSTGEFRYSLSTVPEELVDFAKQSTEVSEPLVAPT---V 78
+L ++F A+ V +++S+ E S S VD Q + P V
Sbjct: 333 NLLNTFEAEENPVVEPTATAAVSSDEL-ISTSEATRHSVDEIAQKPIIDTSEKNPMETFV 391
Query: 79 HPEAILSSTDITSGKIESVPSLIKGGNESLAATKASATEVFAGINETFSDS 129
PEA+ SS D ++ K+ V S ++ E++A+T TE + +T +DS
Sbjct: 392 EPEAVHSSVDESTEKLVVVTSDVENDGENVAST----TEDEITVRDTITDS 438
>At3g53040 late embryogenesis abundant protein - like
Length = 479
Score = 30.8 bits (68), Expect = 1.8
Identities = 35/159 (22%), Positives = 63/159 (39%), Gaps = 13/159 (8%)
Query: 29 AQAIKTVSAWDNSSLSTGEFRYSLSTVPEELVDFAKQSTEVSEPLVAPTVHPEAILSSTD 88
A+A +++A D ++ G+ Y L+ +TE P + +V +A+ + D
Sbjct: 17 AEAAASLAAEDLKDINKGDVTYKLTE--------RTTTTEPERPGLIGSVM-KAVQGTKD 67
Query: 89 ITSGKIESVPSLIKGGNESLAATKASATEVFAGINETFSDSINKGENALRSSVDTATSFI 148
GK + G + +A+ KA+ G E + K + + D A
Sbjct: 68 AVIGKSHDTAESTREGAD-IASEKAAGMRDTTG--EVRDSTAQKTKETADYTADKAREAK 124
Query: 149 DSVVKNATTSADNAFSKVFSAADQTGDLANKKITSFSSE 187
D +AD A K A D+T D K+ +++E
Sbjct: 125 DKTADKTKETADYAAEKAREAKDRTAD-KTKETAEYTAE 162
>At2g34680 auxin-induced protein (AIR9)
Length = 1661
Score = 30.8 bits (68), Expect = 1.8
Identities = 48/181 (26%), Positives = 75/181 (40%), Gaps = 19/181 (10%)
Query: 21 PSLRSSFTAQAIKTVSAWDNSSLSTGEFRYSLSTV-PEELVDFAKQSTEVSEPLVAPTVH 79
P+ SS V+ NS+ E S V P+++ A ++ V L
Sbjct: 63 PTASSSLRVSGTTPVTIRRNSTGGVTENLAGTSKVLPKQVSTTASRTDPVRRSL------ 116
Query: 80 PEAILSSTDITSGKIESVPSL--------IKGGNESLA-ATKASATEVFAGINETFSDSI 130
PE SS S K S PSL + G+ SL +T S ++ + S S+
Sbjct: 117 PELRKSSVSSLSAKTVSKPSLSESKKSVPVSPGSRSLTKSTGFSLSKPESSARPAMSVSV 176
Query: 131 NKGENALRSSVDTATSFIDSVVKNATTSADNAFSKVFSAADQTGDLANKKI--TSFSSEI 188
+ + A SSVD++ S S ++T ++ SKV S + + + I SFSS +
Sbjct: 177 SS-KRAPSSSVDSSGSRTSSGRLHSTLTSGRTVSKVSSPSAGSSPSVSSSIRSKSFSSPL 235
Query: 189 D 189
D
Sbjct: 236 D 236
>At2g30740 putative protein kinase
Length = 366
Score = 30.8 bits (68), Expect = 1.8
Identities = 44/197 (22%), Positives = 82/197 (41%), Gaps = 18/197 (9%)
Query: 63 AKQSTEVSEPLVAPTVHPEAILSSTDITSGKIESVPSLIKG-GNESLAATKASATEVFAG 121
A Q + + +V P EA+ ++ ++ V G++SL + +A
Sbjct: 32 ANQKNQKPQAVVKPEAQKEAL--PIEVPPLSVDEVKEKTDNFGSKSLIGEGSYGRVYYAT 89
Query: 122 INETFSDSINKGENALRSSVDTATSFIDSVVKNATTSADNAFSKVFSAADQTGDLANKKI 181
+N+ + ++ K + A +T T F++ V + +N V D+ + +
Sbjct: 90 LNDGKAVALKKLDVA--PEAETNTEFLNQVSMVSRLKHENLIQLVGYCVDENLRVLAYEF 147
Query: 182 TSFSSEID------GVTSKAPGLVIDVLRRTVVAVESSLSSGASYVVYLYGSAK-EFIPA 234
+ S D GV PG +D L R +AVE+ A + YL+ + I
Sbjct: 148 ATMGSLHDILHGRKGVQGAQPGPTLDWLTRVKIAVEA-----ARGLEYLHEKVQPPVIHR 202
Query: 235 EIRDT-VNIYEDKAAQV 250
+IR + V ++ED A+V
Sbjct: 203 DIRSSNVLLFEDYQAKV 219
>At5g16680 putative protein
Length = 1280
Score = 29.6 bits (65), Expect = 4.0
Identities = 32/126 (25%), Positives = 58/126 (45%), Gaps = 8/126 (6%)
Query: 95 ESVPSLIKGGNESLAATKASATEVFAGINETFSDSINKGENALRSSVDTATSFIDSVVKN 154
+ +PS KGG + ++ + ++ + + +NKG N LR++VD A S KN
Sbjct: 634 KGLPSSQKGGQTAESSDTSGVSDSDLSTTKNVKEDLNKG-NRLRAAVDAALRKKPSFGKN 692
Query: 155 ATTSADNA--FSKVFSAADQT--GDLANKKITSFSSEIDGVTSKAPGL--VIDVLRRTVV 208
+A + V S++++T L +K + S +G+ P L D ++T+V
Sbjct: 693 RVLEQSDASLVANVDSSSEKTLRNQLPSKMHKNHVSH-EGLQGGHPILWPTSDPYKQTIV 751
Query: 209 AVESSL 214
E L
Sbjct: 752 TNEKQL 757
>At3g61450 syntaxin of plants 73 (SYP73)
Length = 263
Score = 29.6 bits (65), Expect = 4.0
Identities = 18/55 (32%), Positives = 32/55 (57%), Gaps = 5/55 (9%)
Query: 153 KNATTSADNAFSKVFSAADQTGDLANKKITSFSSEIDGVTSKAPGLVIDV-LRRT 206
++A S D+AFS+++SA + + +K SSE T+KA + ++ +RRT
Sbjct: 26 RDANVSGDDAFSRLYSAVEYALETVLQKTEDLSSE----TNKAKAVAMNAEIRRT 76
>At2g43230 protein kinase like protein
Length = 406
Score = 29.6 bits (65), Expect = 4.0
Identities = 44/209 (21%), Positives = 88/209 (42%), Gaps = 21/209 (10%)
Query: 52 LSTVPEELVDFAKQSTEVSEPLVAPTV-HPEAILSSTDITSGKIESVPSLIKG-GNESLA 109
L T P D+ + + + P+ P + P I D+ + + + + G+++L
Sbjct: 62 LKTPPTRHYDYGRNNKKTPAPVKPPVLKEPPPI----DVPAMSLVELKEKTQNFGSKALI 117
Query: 110 ATKASATEVFAGINETFSDSINKGENALRSSVDTATSFIDSVVKNATTSADNAFSKVFSA 169
+ +A N+ + ++ K +NA S +T F+ V K + +DN +
Sbjct: 118 GEGSYGRVYYANFNDGKAVAVKKLDNA--SEPETNVEFLTQVSKVSRLKSDNFVQLLGYC 175
Query: 170 ADQTGDLANKKITSFSSEID------GVTSKAPGLVIDVLRRTVVAVESSLSSGASYVVY 223
+ + + + S D GV PG ++ ++R VAV++ A + Y
Sbjct: 176 VEGNLRVLAYEFATMRSLHDILHGRKGVQGAQPGPTLEWMQRVRVAVDA-----AKGLEY 230
Query: 224 LYGSAK-EFIPAEIRDT-VNIYEDKAAQV 250
L+ + I +IR + V I+ED A++
Sbjct: 231 LHEKVQPAVIHRDIRSSNVLIFEDFKAKI 259
>At2g41450 hypothetical protein
Length = 991
Score = 29.6 bits (65), Expect = 4.0
Identities = 28/139 (20%), Positives = 56/139 (40%), Gaps = 9/139 (6%)
Query: 6 GVVEENVHLGLYHGTPSLRSSFTAQAIKTVSAWDNSSLSTGEFRYSLSTVPEELVDFAKQ 65
G ++ G T +L ++ + A +W S+ G F S + + K
Sbjct: 368 GTTSTHIVTGKVRKTLNLCTALCSGAWIVSPSWLKESVREGRFANEASHILHDEDYQLKY 427
Query: 66 STEVSEPLVAPTVHPEAILSSTDITSGKIESVPSLIKGGNESLAATKASATEVFAGIN-- 123
T++ ++ P ++L DI G P++ S A K++ V +G+N
Sbjct: 428 DTDLKSTVLRAKARPNSLLKGYDICVG-----PNIELPIKTSSAIIKSAGGNVISGVNKG 482
Query: 124 --ETFSDSINKGENALRSS 140
+TF +++ ++R S
Sbjct: 483 KAKTFRIALSLAHGSVRVS 501
>At5g57610 putative protein
Length = 1054
Score = 28.9 bits (63), Expect = 6.8
Identities = 27/116 (23%), Positives = 44/116 (37%), Gaps = 11/116 (9%)
Query: 15 GLYHGTPSLRSSFTAQAIKTVSAWDNSSLSTGEFRYSLSTVPEELVDFAKQSTEVSEPLV 74
G+ G+P S F + ++ N L F + VP E + +V EPL
Sbjct: 552 GVLVGSPQRISGFDGMSSLGQPSYPNPHLQDRAFPLDPNWVPSENPTVHNEHLQVREPLP 611
Query: 75 APTVHPEAILSSTDITSGKIESVPSLIKGGNESLAATKASATEVFAGINETFSDSI 130
P L T++T+ I P + S+ + A E F +N S+ +
Sbjct: 612 GP-------LLQTNLTAAPIMQTPVM----QTSVESKLAQGGEQFNYVNTGISNGV 656
>At5g44560 unknown protein
Length = 222
Score = 28.9 bits (63), Expect = 6.8
Identities = 32/137 (23%), Positives = 57/137 (41%), Gaps = 18/137 (13%)
Query: 77 TVHPEAILSSTDITSGKIESVPSLIKGGNESLAATK------------ASATEVFAGINE 124
T H +A+ +ST I+SG + +++ N+ +A TK A ++E
Sbjct: 86 TTHTQALYASTSISSGMKGATKAMV-AMNKQMAPTKQAKVIKDFQKQSAQLDMTIEMMSE 144
Query: 125 TFSDSINKGENALRSSVDTATSFIDSV---VKNATTSADNAFSKVFSAADQTGDLANKKI 181
++++K E A + D +D + V + +SA +AA A K
Sbjct: 145 AIDETLDKDE-AEEETEDLTNQVLDEIGVGVASQLSSAPKGRIATKTAAPPASTAATNK- 202
Query: 182 TSFSSEIDGVTSKAPGL 198
S SSE+D + + L
Sbjct: 203 NSESSEVDELEKRLASL 219
>At2g36500 unknown protein (At2g36500)
Length = 536
Score = 28.9 bits (63), Expect = 6.8
Identities = 32/134 (23%), Positives = 56/134 (40%), Gaps = 16/134 (11%)
Query: 76 PTVHPEAILSSTDITSGKIESVPSLIKGGNESLAATKASATEVFAGINETFSDSINKGEN 135
P V +++ DIT +++ + K + A A + N F D++ E
Sbjct: 161 PVVENGEVIALLDITKCLYDAISRMEKAAEQGSALATAVEERHWGSGNFAFIDTLR--ER 218
Query: 136 ALRSSVDTATSFIDSVVKNATTSADNAFSKVFSAADQTGDL-ANKKITSFSSEIDGV-TS 193
+ ++ T V +N + +A VF A+ + DL N I + ++I G+ TS
Sbjct: 219 MFKPALSTI------VTENTKVALVSASDPVFVASKKMRDLRVNSVIIAVGNKIHGILTS 272
Query: 194 KAPGLVIDVLRRTV 207
K D+L R V
Sbjct: 273 K------DILMRVV 280
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.333 0.143 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,939,835
Number of Sequences: 26719
Number of extensions: 332538
Number of successful extensions: 1542
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 1533
Number of HSP's gapped (non-prelim): 22
length of query: 494
length of database: 11,318,596
effective HSP length: 103
effective length of query: 391
effective length of database: 8,566,539
effective search space: 3349516749
effective search space used: 3349516749
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146683.17