BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146630.4 + phase: 0
(100 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g06270 unknown protein 125 3e-30
At3g11600 unknown protein 116 2e-27
At5g22270 unknown protein 78 7e-16
At1g16500 unknown protein 50 2e-07
At1g79160 unknown protein 42 5e-05
At1g28070 hypothetical protein 35 0.005
At2g33510 unknown protein 33 0.018
At1g78170 unknown protein 33 0.031
At4g32710 putative protein kinase 32 0.070
At5g63130 unknown protein 31 0.12
At4g08910 unknown protein 30 0.20
At3g18810 protein kinase, putative 30 0.27
At4g34440 serine/threonine protein kinase - like 29 0.35
At1g72390 unknown protein 29 0.45
At3g48050 putative protein 28 0.77
At3g12440 hypothetical protein 28 0.77
At2g16720 DNA-binding protein 28 0.77
At5g64360 unknown protein 28 1.0
At3g52710 unknown protein 28 1.0
At3g05330 unknown protein 28 1.0
>At5g06270 unknown protein
Length = 122
Score = 125 bits (315), Expect = 3e-30
Identities = 74/110 (67%), Positives = 82/110 (74%), Gaps = 17/110 (15%)
Query: 1 MSRRNGSGPKLDLKLNLSPPRVN-RRMESSPTRSASVSP---PSSCVSSENG-------- 48
MSRR+ PKL+LKLNLSPP + RRM SP+RSA+ SP PSSCVSSE
Sbjct: 1 MSRRS---PKLELKLNLSPPTSSQRRMVRSPSRSATTSPTSPPSSCVSSEMNQDEPSVRY 57
Query: 49 SSSPEATSMLLVGCPRCLMYVMLSENDPKCPKCHSTVLLDFL--NAENNN 96
S+SPE TSM+LVGCPRCLMYVMLSE+DPKCPKC STVLLDFL NA N N
Sbjct: 58 STSPETTSMVLVGCPRCLMYVMLSEDDPKCPKCKSTVLLDFLHENATNAN 107
>At3g11600 unknown protein
Length = 117
Score = 116 bits (290), Expect = 2e-27
Identities = 63/94 (67%), Positives = 70/94 (74%), Gaps = 5/94 (5%)
Query: 1 MSRRNGSGPKLDLKLNLSPPRVNRRMES---SPTRSASVSPPSSCVSSENGSSSPEA-TS 56
MSRRN +GPKL+L+LNLSPP S SP RS + SP SSCVSSE E TS
Sbjct: 1 MSRRNKNGPKLELRLNLSPPPSQASQMSLVRSPNRSNTTSP-SSCVSSETNQEENETITS 59
Query: 57 MLLVGCPRCLMYVMLSENDPKCPKCHSTVLLDFL 90
M+LVGCPRCLMYVMLS++DPKCPKC STVLLDFL
Sbjct: 60 MVLVGCPRCLMYVMLSDDDPKCPKCKSTVLLDFL 93
>At5g22270 unknown protein
Length = 93
Score = 78.2 bits (191), Expect = 7e-16
Identities = 47/101 (46%), Positives = 63/101 (61%), Gaps = 11/101 (10%)
Query: 1 MSRRNGSGPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLLV 60
M++ S LDLKLN+SP ++SS +S SS S E EA SM++V
Sbjct: 1 MNQEAASNLGLDLKLNISPS-----LDSSLLTESS---SSSLCSEEAEGGGGEAKSMVVV 52
Query: 61 GCPRCLMYVMLS-ENDPKCPKCHSTVLLDFLNAENNNNKKS 100
GCP C+MY++ S ENDP+CP+C+S VLLDFL N++KKS
Sbjct: 53 GCPNCIMYIITSLENDPRCPRCNSHVLLDFLT--GNHSKKS 91
>At1g16500 unknown protein
Length = 259
Score = 50.1 bits (118), Expect = 2e-07
Identities = 20/39 (51%), Positives = 28/39 (71%)
Query: 49 SSSPEATSMLLVGCPRCLMYVMLSENDPKCPKCHSTVLL 87
+S+ A S + GCP CL YV +++N+PKCP+CHS V L
Sbjct: 207 ASATVAASQVAAGCPGCLSYVFVAKNNPKCPRCHSFVPL 245
>At1g79160 unknown protein
Length = 239
Score = 42.0 bits (97), Expect = 5e-05
Identities = 16/32 (50%), Positives = 23/32 (71%)
Query: 56 SMLLVGCPRCLMYVMLSENDPKCPKCHSTVLL 87
S ++ GCP CL YV++ N+PKCP+C + V L
Sbjct: 191 SPVVAGCPGCLSYVLVMMNNPKCPRCDTIVPL 222
>At1g28070 hypothetical protein
Length = 176
Score = 35.4 bits (80), Expect = 0.005
Identities = 22/62 (35%), Positives = 30/62 (47%), Gaps = 1/62 (1%)
Query: 28 SSPTRSASVSPPSSCVSSENGSSSPEATSMLLVGCPRCLMYVMLSENDPKCPKCHSTVLL 87
SS S S SS S + + +++ GC CLMY M+ + CPKC +T LL
Sbjct: 108 SSYYESEESSSESSPSSRKYHKEEQDEDVLVVAGCKACLMYFMVPKLFKDCPKC-ATQLL 166
Query: 88 DF 89
F
Sbjct: 167 HF 168
>At2g33510 unknown protein
Length = 189
Score = 33.5 bits (75), Expect = 0.018
Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 6/60 (10%)
Query: 33 SASVSPPSSCVS------SENGSSSPEATSMLLVGCPRCLMYVMLSENDPKCPKCHSTVL 86
S+S S PSS + E E +++ GC C MY M+ + CPKC + +L
Sbjct: 120 SSSESSPSSRENHKEEEEEEEEEEEEEEDVLVVAGCKACFMYFMVPKLVEDCPKCAAQLL 179
>At1g78170 unknown protein
Length = 221
Score = 32.7 bits (73), Expect = 0.031
Identities = 28/107 (26%), Positives = 38/107 (35%), Gaps = 43/107 (40%)
Query: 11 LDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSP------------------ 52
LDL+LNLSP SP+R+ + SS + +E SSS
Sbjct: 98 LDLELNLSP-------SGSPSRTTTKKYESSSIHNETVSSSKIKVLTNTSKKSIIGTGLS 150
Query: 53 ------------------EATSMLLVGCPRCLMYVMLSENDPKCPKC 81
+ M+ C +C M VML + P CP C
Sbjct: 151 RSPSWLAFEGGDDNDVDHKGQEMVTTVCMKCHMLVMLCTSTPVCPNC 197
>At4g32710 putative protein kinase
Length = 731
Score = 31.6 bits (70), Expect = 0.070
Identities = 19/61 (31%), Positives = 24/61 (39%)
Query: 3 RRNGSGPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLLVGC 62
RR+G P +N SPP + S P S PP S + S+ P S V
Sbjct: 164 RRSGPKPSFPPPINSSPPNPSPNTPSLPETSPPPKPPLSTTPFPSSSTPPPKKSPAAVTL 223
Query: 63 P 63
P
Sbjct: 224 P 224
>At5g63130 unknown protein
Length = 192
Score = 30.8 bits (68), Expect = 0.12
Identities = 18/38 (47%), Positives = 21/38 (54%), Gaps = 2/38 (5%)
Query: 17 LSPPRVNRRMESSPTRSASVSP--PSSCVSSENGSSSP 52
LSPPR + + ESSP+ S SP P S S S SP
Sbjct: 109 LSPPRSSHKTESSPSSSGDRSPKSPFSVTPSPPNSPSP 146
>At4g08910 unknown protein
Length = 212
Score = 30.0 bits (66), Expect = 0.20
Identities = 13/40 (32%), Positives = 17/40 (42%)
Query: 42 CVSSENGSSSPEATSMLLVGCPRCLMYVMLSENDPKCPKC 81
C +G M+ C +C M VML + P CP C
Sbjct: 150 CCDRGDGVGGRSEEEMVARVCMKCHMLVMLCKASPACPNC 189
>At3g18810 protein kinase, putative
Length = 700
Score = 29.6 bits (65), Expect = 0.27
Identities = 18/61 (29%), Positives = 30/61 (48%), Gaps = 6/61 (9%)
Query: 2 SRRNGSGPKLDLKLNLSPPRVNRRME-SSPTRSASVSPPSSCVSSENGSSSPEATSMLLV 60
++ NG G N SPP +R + +SP+ +++PP S N S + E + +V
Sbjct: 135 NQNNGGGSN-----NRSPPPPSRNSDRNSPSPPRALAPPRSSGGGSNSSGNNEPNTAAIV 189
Query: 61 G 61
G
Sbjct: 190 G 190
>At4g34440 serine/threonine protein kinase - like
Length = 670
Score = 29.3 bits (64), Expect = 0.35
Identities = 14/33 (42%), Positives = 17/33 (51%)
Query: 7 SGPKLDLKLNLSPPRVNRRMESSPTRSASVSPP 39
S P D+ + SPP E+SP S S SPP
Sbjct: 45 SAPPPDISASFSPPPAPPTQETSPPTSPSSSPP 77
Score = 28.1 bits (61), Expect = 0.77
Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 12/64 (18%)
Query: 2 SRRNGSGPKLDLKLNLSPPRVNRRMES---SPTRSASVSPPSSCVSSENGSSS-PEATSM 57
+ R+GS P SPP R SP+ S+SPP + S++ S + P+A
Sbjct: 137 NNRDGSTP--------SPPSSGNRTSGDGGSPSPPRSISPPQNSGDSDSSSGNHPQANIG 188
Query: 58 LLVG 61
L++G
Sbjct: 189 LIIG 192
>At1g72390 unknown protein
Length = 1088
Score = 28.9 bits (63), Expect = 0.45
Identities = 15/56 (26%), Positives = 28/56 (49%)
Query: 4 RNGSGPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLL 59
+ S ++ + +SP +VN+R SP S+ P S + E +SP+ +S +
Sbjct: 1008 QQSSPQQMSQRTPMSPQQVNQRTPMSPQISSGAMHPMSTSNLEGCPASPQLSSQTM 1063
>At3g48050 putative protein
Length = 1613
Score = 28.1 bits (61), Expect = 0.77
Identities = 17/52 (32%), Positives = 22/52 (41%)
Query: 12 DLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLLVGCP 63
D+ +NL M SP S SVS P + V +EN + M G P
Sbjct: 772 DVGMNLLASVAADEMSKSPVASPSVSQPPNSVMNENSTVGNNTKLMASDGLP 823
>At3g12440 hypothetical protein
Length = 353
Score = 28.1 bits (61), Expect = 0.77
Identities = 19/49 (38%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Query: 20 PRVNRRMESSPTRSASVSPPSSCVS-SENGSSSPEATSMLLVGCPRCLM 67
P V R S P R SVSPP+ S S S P A ++ L RC++
Sbjct: 181 PLVRYRSVSPPARYLSVSPPARYRSVSPPARSDPPANTLQLCVGNRCII 229
Score = 25.0 bits (53), Expect = 6.5
Identities = 13/29 (44%), Positives = 19/29 (64%), Gaps = 1/29 (3%)
Query: 16 NLSPPRVNRRMESSPTRSASVSPPSSCVS 44
++SPP V R +S P + SVSPP+ +S
Sbjct: 151 SVSPP-VRYRSDSPPAQFRSVSPPARYLS 178
>At2g16720 DNA-binding protein
Length = 269
Score = 28.1 bits (61), Expect = 0.77
Identities = 18/51 (35%), Positives = 25/51 (48%)
Query: 8 GPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSML 58
GP L+L+L +SPP N+R S+ T S SSE E +S +
Sbjct: 187 GPDLNLELRISPPWQNQREISTCTASRFYMENDMECSSETVKCQTENSSSI 237
>At5g64360 unknown protein
Length = 464
Score = 27.7 bits (60), Expect = 1.0
Identities = 18/63 (28%), Positives = 26/63 (40%), Gaps = 5/63 (7%)
Query: 23 NRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLLVGCPRCLMYV----MLSENDPKC 78
++ + P+ S +V PP S S+ P ATS CP C + E KC
Sbjct: 161 HQNFQWEPSSSTAVYPPPRSQSQAGTSADPMATS-FWTACPYCFVLFEYPKAYEECTLKC 219
Query: 79 PKC 81
+C
Sbjct: 220 QEC 222
>At3g52710 unknown protein
Length = 289
Score = 27.7 bits (60), Expect = 1.0
Identities = 11/25 (44%), Positives = 15/25 (60%)
Query: 19 PPRVNRRMESSPTRSASVSPPSSCV 43
P R+ R++S PT S SP SC+
Sbjct: 132 PKRIGSRLKSDPTLSEPCSPKVSCI 156
>At3g05330 unknown protein
Length = 444
Score = 27.7 bits (60), Expect = 1.0
Identities = 21/57 (36%), Positives = 29/57 (50%), Gaps = 11/57 (19%)
Query: 4 RNGSGPKLDLKLNLSPPRVNRRMESSPTRS---------ASVSPPSSCVSSENGSSS 51
RNGS L K ++SP RV + SPTR+ AS+SP C S+ S++
Sbjct: 365 RNGSN--LARKSSISPKRVTLQAFLSPTRNGNFCKKSPKASISPTRVCNKSQKLSTA 419
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.310 0.125 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,541,463
Number of Sequences: 26719
Number of extensions: 97026
Number of successful extensions: 511
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 47
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 441
Number of HSP's gapped (non-prelim): 83
length of query: 100
length of database: 11,318,596
effective HSP length: 76
effective length of query: 24
effective length of database: 9,287,952
effective search space: 222910848
effective search space used: 222910848
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146630.4