BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146584.2 - phase: 0 /pseudo
(1452 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g10780 pseudogene 119 1e-26
At4g16910 retrotransposon like protein 115 1e-25
At4g03840 putative transposon protein 114 5e-25
At2g07660 putative retroelement pol polyprotein 99 1e-20
At3g31970 hypothetical protein 99 2e-20
At2g14650 putative retroelement pol polyprotein 98 4e-20
At2g04670 putative retroelement pol polyprotein 96 2e-19
At4g10580 putative reverse-transcriptase -like protein 93 1e-18
At1g36120 putative reverse transcriptase gb|AAD22339.1 87 7e-17
At2g06470 putative retroelement pol polyprotein 86 2e-16
At3g29490 hypothetical protein 83 1e-15
At3g11970 hypothetical protein 77 9e-14
At1g36590 hypothetical protein 77 9e-14
At4g07830 putative reverse transcriptase 72 3e-12
At2g14640 putative retroelement pol polyprotein 56 1e-07
At2g05610 putative retroelement pol polyprotein 54 5e-07
At1g35370 hypothetical protein 39 0.017
At2g06190 putative Ty3-gypsy-like retroelement pol polyprotein 36 0.14
At3g31480 hypothetical protein 35 0.32
At1g42190 hypothetical protein 35 0.41
>At2g10780 pseudogene
Length = 1611
Score = 119 bits (299), Expect = 1e-26
Identities = 55/117 (47%), Positives = 79/117 (67%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
K++ P+++GPY+++ERVG VAY++ LPP L+ HNVFHVSQLRK + D ++ +
Sbjct: 1466 KKLSPRYVGPYKVIERVGAVAYKLDLPPKLNAFHNVFHVSQLRKCLSDQEESVEDIPPGL 1525
Query: 1386 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWTGATGESLTWELESKMLESYPELF 1442
++N+TVE PVRI DR K RGK L++V+W E TWE E+KM ++PE F
Sbjct: 1526 KENMTVEAWPVRIMDRMTKGTRGKARDLLKVLWNCRGREEYTWETENKMKANFPEWF 1582
>At4g16910 retrotransposon like protein
Length = 687
Score = 115 bits (289), Expect = 1e-25
Identities = 52/117 (44%), Positives = 79/117 (67%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
K++ P+++GPY+++ERVG VAY++ LPP L HNVFHVSQLRK + + ++ +
Sbjct: 550 KKLRPRYVGPYKVIERVGAVAYKLDLPPKLDAFHNVFHVSQLRKCLSEQEESMEDVPPGL 609
Query: 1386 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWTGATGESLTWELESKMLESYPELF 1442
++N+TVE PVRI D+ K RGK + L++++W E TWE E+KM ++PE F
Sbjct: 610 KENMTVEAWPVRIMDQMKKGTRGKSMDLLKILWNCGGREEYTWETETKMKANFPEWF 666
>At4g03840 putative transposon protein
Length = 973
Score = 114 bits (284), Expect = 5e-25
Identities = 52/117 (44%), Positives = 78/117 (66%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
K++ P+++GPY+++ERVG VAY++ LPP L+ HNVFHVSQLRK + + ++ +
Sbjct: 828 KKLSPRYVGPYKVIERVGAVAYKLDLPPKLNAFHNVFHVSQLRKCLSNQEESVEDVPPGL 887
Query: 1386 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWTGATGESLTWELESKMLESYPELF 1442
++N+TVE PV+I DR K RGK L++V+W E TWE E+KM ++ E F
Sbjct: 888 KENMTVEAWPVQIMDRMTKGTRGKSRDLLKVLWNCGGREQYTWETENKMKANFSEWF 944
>At2g07660 putative retroelement pol polyprotein
Length = 949
Score = 99.4 bits (246), Expect = 1e-20
Identities = 45/92 (48%), Positives = 66/92 (70%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
K++ P+++GPY+++ERVG VAY++ LPP L+ HNVFHVSQLRKY+ D ++ +
Sbjct: 858 KKLSPRYVGPYKVIERVGAVAYKLDLPPKLNVFHNVFHVSQLRKYLSDQEESVEDIPPGL 917
Query: 1386 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVV 1417
++N+TVE PVRI DR K RGK L++V+
Sbjct: 918 KENMTVEAWPVRIMDRMSKGTRGKSRDLLKVL 949
>At3g31970 hypothetical protein
Length = 1329
Score = 99.0 bits (245), Expect = 2e-20
Identities = 51/118 (43%), Positives = 78/118 (65%), Gaps = 4/118 (3%)
Query: 1327 EVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQVR 1386
++ P+++GP++I+ERVG VAYR+ LP + H VFHVS LRK + V+ ++
Sbjct: 1208 KLTPRYMGPFRIVERVGPVAYRLELPDVMRAFHKVFHVSMLRKCLHKDDEVLAKILEDLQ 1267
Query: 1387 DNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWT--GATGESLTWELESKMLESYPELF 1442
N+T+E PVRI +R++K LR K+IPL++V+W G T E TWE E++M S+ + F
Sbjct: 1268 PNMTLEARPVRILERRIKELRRKKIPLIKVLWNCDGVTEE--TWEPEARMKASFKKWF 1323
>At2g14650 putative retroelement pol polyprotein
Length = 1328
Score = 97.8 bits (242), Expect = 4e-20
Identities = 49/118 (41%), Positives = 77/118 (64%), Gaps = 4/118 (3%)
Query: 1327 EVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQVR 1386
++ P+++GP++I+ERVG VAYR+ LP + H VFHVS LRK + V+ ++
Sbjct: 1210 KLSPRYMGPFRIVERVGPVAYRLELPDVMRAFHKVFHVSMLRKCLHKDDEVLAKIPEDLQ 1269
Query: 1387 DNLTVETLPVRIDDRKVKTLRGKEIPLVRVVW--TGATGESLTWELESKMLESYPELF 1442
N+T+E PVR+ +R++K LR K+IPL++V+W G T E TWE E++M + + F
Sbjct: 1270 PNMTLEARPVRVLERRIKELRRKKIPLIKVLWDCDGVTKE--TWEPEARMKARFKKWF 1325
>At2g04670 putative retroelement pol polyprotein
Length = 1411
Score = 95.9 bits (237), Expect = 2e-19
Identities = 51/125 (40%), Positives = 79/125 (62%), Gaps = 5/125 (4%)
Query: 1320 RTCFEVKEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQ 1379
R+ E K + P+++GP++I+ERVG VAYR+ LP + H VFHV LRK + V+
Sbjct: 1284 RSILETK-LSPRYMGPFRIVERVGPVAYRLELPDVMRAFHKVFHVLMLRKCLHKDDEVLV 1342
Query: 1380 SDDVQVRDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVW--TGATGESLTWELESKMLES 1437
++ N+T+E PVR+ +R++K LR K+IPL++V+W G T E TWE E++M
Sbjct: 1343 KIPEDLQPNMTLEARPVRVLERRIKELRRKKIPLIKVLWDCDGVTEE--TWEPEARMKAR 1400
Query: 1438 YPELF 1442
+ + F
Sbjct: 1401 FKKWF 1405
>At4g10580 putative reverse-transcriptase -like protein
Length = 1240
Score = 93.2 bits (230), Expect = 1e-18
Identities = 47/118 (39%), Positives = 75/118 (62%), Gaps = 4/118 (3%)
Query: 1327 EVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQVR 1386
++ P+++GP++I+ERV VAYR+ LP + H VFHVS LRK + + ++
Sbjct: 1119 KLSPRYMGPFKIVERVEPVAYRLELPDVMRAFHKVFHVSMLRKCLHKDDEALAKIPEDLQ 1178
Query: 1387 DNLTVETLPVRIDDRKVKTLRGKEIPLVRVVW--TGATGESLTWELESKMLESYPELF 1442
N+T+E PVR+ +R++K LR K+IPL++V+W G T E TWE E++M + + F
Sbjct: 1179 PNMTLEARPVRVLERRIKELRQKKIPLIKVLWDCDGVTEE--TWEPEARMKARFKKWF 1234
>At1g36120 putative reverse transcriptase gb|AAD22339.1
Length = 1235
Score = 87.0 bits (214), Expect = 7e-17
Identities = 48/128 (37%), Positives = 78/128 (60%), Gaps = 4/128 (3%)
Query: 1317 DRCRTCFEVKEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSH 1376
DR R+ + + + +F +I+ERVG VAYR+ LP + HNVFHVS LRK +
Sbjct: 1104 DRQRSYADKRRRELEFEVGTEIVERVGPVAYRLELPDVMRAFHNVFHVSMLRKCLHKDDE 1163
Query: 1377 VIQSDDVQVRDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVW--TGATGESLTWELESKM 1434
V+ ++ N+T+E PVR+ +R++K +R K+IP+++V+W G T E TWE E+++
Sbjct: 1164 VLAKIPEDLQPNMTLEARPVRVLERRIKEVRRKKIPMIKVLWDCDGVTEE--TWEPEARI 1221
Query: 1435 LESYPELF 1442
+ + F
Sbjct: 1222 KARFKKWF 1229
>At2g06470 putative retroelement pol polyprotein
Length = 899
Score = 85.5 bits (210), Expect = 2e-16
Identities = 37/75 (49%), Positives = 55/75 (73%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
K++ P+++GPY+++ERVG VAY++ LPP L+ HNVFHVSQLRK + D ++ +
Sbjct: 825 KKLSPRYVGPYKVIERVGAVAYKLDLPPKLNAFHNVFHVSQLRKCLSDQEESVEDVPPGL 884
Query: 1386 RDNLTVETLPVRIDD 1400
++N+TVE PVRI D
Sbjct: 885 KENMTVEAWPVRIMD 899
>At3g29490 hypothetical protein
Length = 438
Score = 83.2 bits (204), Expect = 1e-15
Identities = 43/114 (37%), Positives = 71/114 (61%), Gaps = 4/114 (3%)
Query: 1331 KFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQVRDNLT 1390
+++GP++I+ERVG VAY + LP + H VFHVS LRK + V+ ++ N+T
Sbjct: 224 RYMGPFRIVERVGPVAYMLELPDVMRAFHKVFHVSMLRKCLHKDDEVLAKIPEDLQPNMT 283
Query: 1391 VETLPVRIDDRKVKTLRGKEIPLVRVVW--TGATGESLTWELESKMLESYPELF 1442
+E VR+ +R++K L+ K+I L++V+W G T E TW+ E++M + + F
Sbjct: 284 LEARQVRVLERRIKELQRKKISLIKVLWDCDGVTEE--TWQPEARMKARFKKWF 335
>At3g11970 hypothetical protein
Length = 1499
Score = 76.6 bits (187), Expect = 9e-14
Identities = 42/118 (35%), Positives = 70/118 (58%), Gaps = 4/118 (3%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
+++ PK+ GPY+I++R G VAY++ LP + S +H VFHVSQL+ V + S + V +
Sbjct: 1386 QKLSPKYFGPYKIIDRCGEVAYKLALPSY-SQVHPVFHVSQLKVLVGNVSTTVHLPSV-M 1443
Query: 1386 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWTGATGESLTWELESKMLESYPELFA 1443
+D E +P ++ +RK+ +GK + V V W+ E TWE + +++PE A
Sbjct: 1444 QD--VFEKVPEKVVERKMVNRQGKAVTKVLVKWSNEPLEEATWEFLFDLQKTFPEFEA 1499
>At1g36590 hypothetical protein
Length = 1499
Score = 76.6 bits (187), Expect = 9e-14
Identities = 42/118 (35%), Positives = 70/118 (58%), Gaps = 4/118 (3%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
+++ PK+ GPY+I++R G VAY++ LP + S +H VFHVSQL+ V + S + V +
Sbjct: 1386 QKLSPKYFGPYKIIDRCGEVAYKLALPSY-SQVHPVFHVSQLKVLVGNVSTTVHLPSV-M 1443
Query: 1386 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWTGATGESLTWELESKMLESYPELFA 1443
+D E +P ++ +RK+ +GK + V V W+ E TWE + +++PE A
Sbjct: 1444 QD--VFEKVPEKVVERKMVNRQGKAVTKVLVKWSNEPLEEATWEFLFDLQKTFPEFEA 1499
>At4g07830 putative reverse transcriptase
Length = 611
Score = 71.6 bits (174), Expect = 3e-12
Identities = 40/105 (38%), Positives = 63/105 (59%), Gaps = 4/105 (3%)
Query: 1340 ERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQVRDNLTVETLPVRID 1399
+RVG VA+R+ L + H VFHVS LRK + V+ ++ N+T+E PVR+
Sbjct: 467 QRVGPVAFRLELSDVMRAFHKVFHVSMLRKCLHKDDEVLAKIPEDLQPNMTLEARPVRVL 526
Query: 1400 DRKVKTLRGKEIPLVRVV--WTGATGESLTWELESKMLESYPELF 1442
+R++K LR K+IPL++V+ G T E TWE E+++ + + F
Sbjct: 527 ERRIKELRRKKIPLIKVLRNCDGVTEE--TWEPEARLKARFKKWF 569
>At2g14640 putative retroelement pol polyprotein
Length = 945
Score = 56.2 bits (134), Expect = 1e-07
Identities = 34/115 (29%), Positives = 60/115 (51%), Gaps = 2/115 (1%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
+++ +F GP+Q+ + G VAYR+ LP + +H VFHVS L+ +V D +
Sbjct: 822 QKLSHRFYGPFQVASKHGEVAYRLTLPEG-TRIHPVFHVSLLKPWVGD-GEPDMGQLPPL 879
Query: 1386 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWTGATGESLTWELESKMLESYPE 1440
R+N ++ P + + + ++ K + + V W G E TWE ++ S+PE
Sbjct: 880 RNNGELKLQPTAVLEVRWRSQDKKRVADLLVQWEGLHIEDATWEEYDQLAASFPE 934
>At2g05610 putative retroelement pol polyprotein
Length = 780
Score = 54.3 bits (129), Expect = 5e-07
Identities = 27/67 (40%), Positives = 46/67 (68%), Gaps = 4/67 (5%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKY---VPDPSHVIQSDD 1382
+++ PK+ GPY++++R G VAY++ LP + S +H VFHVSQLR V +H+++
Sbjct: 714 QKLSPKYFGPYKVIDRCGEVAYKLQLPAN-SQVHPVFHVSQLRVLVGTVTTSTHLLRCYL 772
Query: 1383 VQVRDNL 1389
+ +R+NL
Sbjct: 773 MSLRENL 779
>At1g35370 hypothetical protein
Length = 1447
Score = 39.3 bits (90), Expect = 0.017
Identities = 28/104 (26%), Positives = 44/104 (41%), Gaps = 27/104 (25%)
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
+++ PK+ GPY+I+E+ G V + NV +QL +PD
Sbjct: 1357 QKLSPKYFGPYKIIEKCGEVM-----------VGNVTTSTQLPSVLPD------------ 1393
Query: 1386 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWTGATGESLTWE 1429
E P I +RK+ +G+ +V V W G E TW+
Sbjct: 1394 ----IFEKAPEYILERKLVKRQGRAATMVLVKWIGEPVEEATWK 1433
>At2g06190 putative Ty3-gypsy-like retroelement pol polyprotein
Length = 280
Score = 36.2 bits (82), Expect = 0.14
Identities = 16/47 (34%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Query: 1327 EVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPD 1373
+V P+ GP+++L ++ AY V LP + N+ N F+V+ + +Y D
Sbjct: 211 KVKPRKYGPFKVLRKINDNAYVVALPKSM-NISNTFNVADIHEYHAD 256
>At3g31480 hypothetical protein
Length = 338
Score = 35.0 bits (79), Expect = 0.32
Identities = 13/22 (59%), Positives = 20/22 (90%)
Query: 1327 EVDPKFIGPYQILERVGTVAYR 1348
++ PK++GP++I+ERVG VAYR
Sbjct: 187 KLSPKYMGPFRIVERVGPVAYR 208
>At1g42190 hypothetical protein
Length = 64
Score = 34.7 bits (78), Expect = 0.41
Identities = 15/45 (33%), Positives = 24/45 (53%)
Query: 1347 YRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQVRDNLTV 1391
Y++ LP + H VFHVS LRK + +VI + +N+ +
Sbjct: 2 YKLDLPTLMDAFHKVFHVSMLRKCITHQENVISEPPPDLLENMMI 46
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.365 0.164 0.591
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,371,132
Number of Sequences: 26719
Number of extensions: 888702
Number of successful extensions: 4482
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 4446
Number of HSP's gapped (non-prelim): 32
length of query: 1452
length of database: 11,318,596
effective HSP length: 112
effective length of query: 1340
effective length of database: 8,326,068
effective search space: 11156931120
effective search space used: 11156931120
T: 11
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.6 bits)
S2: 67 (30.4 bits)
Medicago: description of AC146584.2