BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146571.3 + phase: 0
(444 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g21770 unknown protein (At4g21770) 583 e-167
At3g19440 unknown protein 38 0.010
At5g51140 unknown protein 37 0.029
At1g79190 hypothetical protein 36 0.037
At3g52260 unknown protein 34 0.19
At1g76050 putative RNA pseudouridylate synthase 32 0.92
At1g78910 unknown protein 30 2.7
At3g45530 putative protein 30 3.5
At5g25230 translation Elongation Factor 2 - like protein 29 6.0
At1g06220 elongation factor like protein 29 6.0
>At4g21770 unknown protein (At4g21770)
Length = 472
Score = 583 bits (1504), Expect = e-167
Identities = 273/399 (68%), Positives = 332/399 (82%), Gaps = 8/399 (2%)
Query: 51 YPKYDRLLPCPSHKIPPRIEHLVVSQEGPVL-QYICKALDLPHLFVADLIQFGAVYFALV 109
Y +Y+RL+PCP++ +PPRIEH+VV ++ ++ ++I K LDLP L+VADLI+FGAV++ALV
Sbjct: 73 YHEYNRLMPCPAYNLPPRIEHMVVLEDDVLVSEFISKQLDLPPLYVADLIRFGAVHYALV 132
Query: 110 SPEPPPTATAEQIRIFEQVTEPLVLQKRSSLKGKTIREAQKTFRVTDANQFVEPGTYLRV 169
P+PPPTAT E+I +FE+VT P VL+KRSS+KGKT+REAQKTFRVT NQ+ E GTYLRV
Sbjct: 133 CPKPPPTATPEEIILFEEVTSPSVLKKRSSIKGKTVREAQKTFRVTHTNQYAEAGTYLRV 192
Query: 170 HVHPKRFPRCYEIDWRSRIIAVEESYVVLDKPAGTSVGETTGNIEESCVTFATRALGLTT 229
HVHPKR PRCYEIDW+SRI+AV +SYV+LDKPAGT+VG TT NIEESC TFA+RAL L
Sbjct: 193 HVHPKRSPRCYEIDWKSRIVAVTDSYVILDKPAGTTVGGTTDNIEESCATFASRALDLPE 252
Query: 230 PLITTHQIDNCTEGCVVLARNKEYCSVFHCKIREKKVKKIYLALAASPLPIGIIKHYMRP 289
PL TTHQIDNCTEGCVV AR KEYCSVFH KIR K+VKK+Y ALAA+PLPIGII HYMRP
Sbjct: 253 PLKTTHQIDNCTEGCVVFARTKEYCSVFHTKIRNKEVKKLYRALAAAPLPIGIISHYMRP 312
Query: 290 TNTAPRLISEDCIKGWHICQLEVMDCRKIPWPAIVVQDEY----CGWPSQDYAYECKINL 345
N APRL++ED I+GWH+CQLEV++C+KIPWP + ++ CGW S+++AYEC INL
Sbjct: 313 KNMAPRLVAEDSIQGWHLCQLEVLECKKIPWPDAATEKKHDIEDCGWTSKEFAYECTINL 372
Query: 346 LTGRTHQIRAQFAACKAPIIGDTMYMPAAIAEMINPGLNPFGKYNKDFS-SESEKKTAVI 404
LTG+THQIRAQ AAC AP++GD+MYMPAAIAEM NP NP+GK K ++ E++K+TAV
Sbjct: 373 LTGKTHQIRAQLAACGAPLVGDSMYMPAAIAEMGNPDTNPYGKAKKHYTMEENQKETAVA 432
Query: 405 NWIAQHGKEPSVAIGLQACQISWDDD--EYIYRAGLPWW 441
WI +HGKEP V IGLQACQISWDDD E+ Y AG PWW
Sbjct: 433 EWIDRHGKEPRVGIGLQACQISWDDDDGEHFYEAGTPWW 471
>At3g19440 unknown protein
Length = 477
Score = 38.1 bits (87), Expect = 0.010
Identities = 72/280 (25%), Positives = 115/280 (40%), Gaps = 54/280 (19%)
Query: 186 SRIIAVEESYVVLDKPAGTSVGETTG---NIEE---SCVTFATRALGLTTPLITTHQIDN 239
S ++ + + +VL+KP G +V +G +I+E SC+ F +P + H++D
Sbjct: 183 SLVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSE----SPRLV-HRLDR 237
Query: 240 CTEGCVVLARNKEYCSVFHCKIRE--------------KKVKKIYLALAASPLPIGIIKH 285
G +VLAR + +V H RE K +K+ Y+AL IG
Sbjct: 238 DCSGLLVLARTQTAATVLHSIFREKTTGASAYGVKKNVKSLKRKYMALV-----IGCPPR 292
Query: 286 YMRPTNTAPRLISEDCIKGWHICQLEVMDCRKIPWPAIVVQDEYCGWPSQDYAYE-CKIN 344
+ R + D K I V D ++ + EY S + Y ++
Sbjct: 293 QRGQISAPLRKVVVDDGKSERI---TVNDNGELVSTQHAI-TEYRVIESSPHGYTWLELR 348
Query: 345 LLTGRTHQIRAQFA-ACKAPIIGDTMYMPAA-------IAEMINP---GLNPFGKYNKDF 393
LTGR HQ+R A PI+GD Y A ++ NP +PFG + D
Sbjct: 349 PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSSENNPTKQSSSPFG-LDLDG 407
Query: 394 SSESEKKTAVINWIAQHGKE---PSVAIGLQACQISWDDD 430
S K+ + H K+ P+++ L+ Q+S D D
Sbjct: 408 GDVSSKQP----HLHLHSKQIDLPNISQLLEKMQVSSDSD 443
>At5g51140 unknown protein
Length = 395
Score = 36.6 bits (83), Expect = 0.029
Identities = 42/186 (22%), Positives = 65/186 (34%), Gaps = 13/186 (6%)
Query: 188 IIAVEESYVVLDKPAGTSVGETTGNIEESCVTFATRALGLTTPLITTHQIDNCTEGCVVL 247
I+ E V + KPA V G ++ + A L H++D G +++
Sbjct: 138 ILHQEPDVVTVCKPASVPV-HPCGQYRKNTIVGILDAEHDLGTLFPIHRLDRLVSGLLII 196
Query: 248 ARNKEYCSVFHCKIREKKVKKIYLALAASPLPIG--IIKHYMRPTNTAPRLISEDCIKGW 305
AR F +I VKK Y+A P I+ + + R +ED
Sbjct: 197 ARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMIVDANINYNGSEGRSTAEDANSSG 256
Query: 306 HICQLEVMDCRKIPWPAIVVQDEYCGWPSQDYAYECKINLLTGRTHQIRAQFAACKAPII 365
D +K+ + C+ +TGRTHQIR PI
Sbjct: 257 --------DDKKVKGKPACTKFTRIDTNGTHSLVSCE--PVTGRTHQIRVHLQYTGHPIA 306
Query: 366 GDTMYM 371
D +Y+
Sbjct: 307 NDPLYL 312
>At1g79190 hypothetical protein
Length = 1280
Score = 36.2 bits (82), Expect = 0.037
Identities = 40/201 (19%), Positives = 79/201 (38%), Gaps = 10/201 (4%)
Query: 61 PSHKIPPRIEHLVVSQEGPVLQYICKAL-------DLPHLFVADLIQFGAVYFALVSPEP 113
P+ ++ + HLVV+ V+ IC+ L +P++ A L G + L E
Sbjct: 798 PTVRMKNNVGHLVVANADYVIDSICRQLRHLDLNPHVPNVLAAMLSYIGVAHDILPLLEE 857
Query: 114 PPTATAEQIRIFEQVTEPLVLQKRSSLKGKTIREAQKTFRVTDANQFVEPGTYLRVHVHP 173
P ++++ I + P + G+ + A K ++ +++
Sbjct: 858 PMRLVSQELEIVGRQQHPNLTIPFLKAVGEIVN-ASKNEACLLPDRAKSYSDHVKTKATD 916
Query: 174 KRFPRCYEIDWRSRIIAVEESY--VVLDKPAGTSVGETTGNIEESCVTFATRALGLTTPL 231
R + +I+ EE + ++L+ T G+I SC+ AT L + +
Sbjct: 917 AITSRQERVSNSDKIVEDEEEWENILLELNRSKRYRRTVGSIASSCLIAATPLLASSNQV 976
Query: 232 ITTHQIDNCTEGCVVLARNKE 252
++ EG V LA+ +E
Sbjct: 977 SCLVSLEIIEEGVVALAKVEE 997
>At3g52260 unknown protein
Length = 369
Score = 33.9 bits (76), Expect = 0.19
Identities = 14/42 (33%), Positives = 23/42 (54%)
Query: 342 KINLLTGRTHQIRAQFAACKAPIIGDTMYMPAAIAEMINPGL 383
K+ + +GR HQIR A P++GD +Y+ + +P L
Sbjct: 267 KVEIQSGRPHQIRIHLAYMGHPLVGDPLYVAGGQPKCFDPDL 308
>At1g76050 putative RNA pseudouridylate synthase
Length = 430
Score = 31.6 bits (70), Expect = 0.92
Identities = 14/26 (53%), Positives = 17/26 (64%)
Query: 345 LLTGRTHQIRAQFAACKAPIIGDTMY 370
L TGRTHQIRA P++GD +Y
Sbjct: 332 LETGRTHQIRAHAKYMGVPLLGDEVY 357
>At1g78910 unknown protein
Length = 491
Score = 30.0 bits (66), Expect = 2.7
Identities = 27/102 (26%), Positives = 42/102 (40%), Gaps = 15/102 (14%)
Query: 176 FPRCYEIDWRSRIIAVEES-YVVLDKPAGTSVGETTGN--IEESCVTFATRALG------ 226
+P EI + R++ ++S +VL+KP V GN I S A AL
Sbjct: 168 YPNADEIAYLQRLVRFKDSAIIVLNKPPKLPV---KGNVPIHNSMDALAAAALSFGNDEG 224
Query: 227 ---LTTPLITTHQIDNCTEGCVVLARNKEYCSVFHCKIREKK 265
+ + H++D T G +V+ R KE H + K
Sbjct: 225 PRLVKLTFLGVHRLDRETSGLLVMGRTKESIDYLHSVFSDYK 266
>At3g45530 putative protein
Length = 692
Score = 29.6 bits (65), Expect = 3.5
Identities = 22/92 (23%), Positives = 34/92 (36%), Gaps = 7/92 (7%)
Query: 217 CVTFATRALGLTTPLITTHQIDNCTEGCVVLARNK-EYCS------VFHCKIREKKVKKI 269
C + +T P T H + TEG K C ++HC I + + +
Sbjct: 163 CAALVIDSPEITHPCHTKHPLKLLTEGAPTYTDPKCHLCGEDTGKFIYHCDICKFNLDLV 222
Query: 270 YLALAASPLPIGIIKHYMRPTNTAPRLISEDC 301
P+ + +K + N PRLIS C
Sbjct: 223 CAKTKTPPVALSNLKVHEHTLNLMPRLISFVC 254
>At5g25230 translation Elongation Factor 2 - like protein
Length = 973
Score = 28.9 bits (63), Expect = 6.0
Identities = 11/32 (34%), Positives = 18/32 (55%)
Query: 405 NWIAQHGKEPSVAIGLQACQISWDDDEYIYRA 436
+W+ G + S+ C S+D+D YI+RA
Sbjct: 548 SWVLIEGVDASIMKTATLCNASYDEDVYIFRA 579
>At1g06220 elongation factor like protein
Length = 987
Score = 28.9 bits (63), Expect = 6.0
Identities = 11/32 (34%), Positives = 18/32 (55%)
Query: 405 NWIAQHGKEPSVAIGLQACQISWDDDEYIYRA 436
+W+ G + S+ C S+D+D YI+RA
Sbjct: 562 SWVLIEGVDASIMKTATLCNASYDEDVYIFRA 593
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.137 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,500,373
Number of Sequences: 26719
Number of extensions: 457886
Number of successful extensions: 919
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 904
Number of HSP's gapped (non-prelim): 14
length of query: 444
length of database: 11,318,596
effective HSP length: 102
effective length of query: 342
effective length of database: 8,593,258
effective search space: 2938894236
effective search space used: 2938894236
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146571.3