BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146559.3 + phase: 0
(130 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g02230 putative phloem-specific lectin 125 5e-30
At2g02360 putative phloem-specific lectin 124 1e-29
At5g24560 phloem-specific lectin-like protein 121 1e-28
At2g02340 putative phloem-specific lectin 120 3e-28
At2g02250 lectin-like protein 117 2e-27
At1g09155 unknown protein 107 1e-24
At1g56250 hypothetical protein 103 2e-23
At2g02310 putative phloem-specific lectin 101 1e-22
At2g02320 unknown protein 97 2e-21
At2g02350 SKP1 interacting partner 3 (SKIP3) 96 4e-21
At1g56240 hypothetical protein 95 1e-20
At3g53000 unknown protein 94 1e-20
At2g02300 putative phloem-specific lectin 91 2e-19
At2g02270 putative phloem-specific lectin 88 1e-18
At3g61060 unknown protein 84 2e-17
At1g63090 unknown protein 75 9e-15
At1g12710 unknown protein 74 2e-14
At5g52120 unknown protein 72 1e-13
At1g80110 unknown protein 45 8e-06
At4g19840 lectin like protein 39 7e-04
>At2g02230 putative phloem-specific lectin
Length = 317
Score = 125 bits (315), Expect = 5e-30
Identities = 57/121 (47%), Positives = 86/121 (70%), Gaps = 3/121 (2%)
Query: 7 LPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNIP 66
LPE CI+ ++S T+P DA ++ SK+ +SA+ SD VW FLPS+ SS++ S N
Sbjct: 35 LPEDCISKVISHTSPRDACVVASVSKSVKSAAQSDLVWEMFLPSEYSSLVLQS---ANHL 91
Query: 67 TKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPDS 126
+KK+++L+L+D V++++ KKS +E+ SGKKCYMLSA L I+WGD YW WI++P+S
Sbjct: 92 SKKEIFLSLADNSVLVENGKKSFWVEKASGKKCYMLSAMELTIIWGDSPAYWKWITVPES 151
Query: 127 R 127
+
Sbjct: 152 K 152
>At2g02360 putative phloem-specific lectin
Length = 272
Score = 124 bits (311), Expect = 1e-29
Identities = 59/120 (49%), Positives = 81/120 (67%), Gaps = 3/120 (2%)
Query: 8 PEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNIPT 67
PE CI+ I+S T P DA + SKTF S SD +W +FLP+D S+I P +
Sbjct: 18 PEDCISYIISFTNPRDACVAATVSKTFESTVKSDIIWEKFLPADYESLI---PPSRVFSS 74
Query: 68 KKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPDSR 127
KK+LY +L + PV+ D DKKS+ LE+ SGK+C MLSA +L+I+WGD+ +YW WI +P+SR
Sbjct: 75 KKELYFSLCNDPVLFDDDKKSVWLEKASGKRCLMLSAMNLSIIWGDNPQYWQWIPIPESR 134
>At5g24560 phloem-specific lectin-like protein
Length = 251
Score = 121 bits (303), Expect = 1e-28
Identities = 59/122 (48%), Positives = 81/122 (66%), Gaps = 3/122 (2%)
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNI 65
DLPE CIA ++S T+P DA R+S SK RSA+DS+ W +FLPSD I +S S +
Sbjct: 5 DLPEECIATMISFTSPFDACRISAVSKLLRSAADSNTTWERFLPSDYRMYIDNSLSRFS- 63
Query: 66 PTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPD 125
K L+L + P++I+ + S +E++SGKKC+MLSAR L IVW D +W W+S+PD
Sbjct: 64 --NKQLFLRFCESPLLIEDGRTSFWMEKRSGKKCWMLSARKLDIVWVDSPEFWIWVSIPD 121
Query: 126 SR 127
SR
Sbjct: 122 SR 123
>At2g02340 putative phloem-specific lectin
Length = 305
Score = 120 bits (300), Expect = 3e-28
Identities = 59/127 (46%), Positives = 85/127 (66%), Gaps = 1/127 (0%)
Query: 1 MTEIQDLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSP 60
+ E+ DLPE C++ IVS T+P DA L+ SKTF SA SD VW +F+P + S+IS S
Sbjct: 33 VAELDDLPEECVSIIVSFTSPQDACVLASVSKTFASAVKSDIVWEKFIPPEYESLISQSR 92
Query: 61 SLTNIPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNW 120
+ + +KK+LY AL D+ V+ID KKS+ +E+ + K+C M+SA +LAI WG+ + W W
Sbjct: 93 AFKFL-SKKELYFALCDKSVLIDDGKKSLWIEKANAKRCIMISAMNLAIAWGNSPQSWRW 151
Query: 121 ISMPDSR 127
I P +R
Sbjct: 152 IPDPQAR 158
>At2g02250 lectin-like protein
Length = 305
Score = 117 bits (292), Expect = 2e-27
Identities = 58/123 (47%), Positives = 85/123 (68%), Gaps = 4/123 (3%)
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNI 65
+LPE CI+ I+S T+P DA + SKTF SA +SD+VW++FLPSD SS++ P
Sbjct: 44 NLPEDCISNIISFTSPRDACVAASVSKTFESAVNSDSVWDKFLPSDYSSLV---PPSRVF 100
Query: 66 PTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLS-ARSLAIVWGDDRRYWNWISMP 124
+KK+LY A+ D PV+++ KS LE+++GKKC+MLS +S+ I W +YW WIS+P
Sbjct: 101 SSKKELYFAICDNPVLVEDGGKSFWLEKENGKKCFMLSPKKSMWITWVSTPQYWRWISIP 160
Query: 125 DSR 127
++R
Sbjct: 161 EAR 163
>At1g09155 unknown protein
Length = 289
Score = 107 bits (268), Expect = 1e-24
Identities = 55/124 (44%), Positives = 76/124 (60%), Gaps = 2/124 (1%)
Query: 7 LPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNI- 65
LPE C+A I+S TTP D + S FR A DSD VW +FLP+D +IS S I
Sbjct: 3 LPEACVATILSFTTPADTISSAAVSSVFRVAGDSDFVWEKFLPTDYCHVISRSTDPHRIF 62
Query: 66 PTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPD 125
+KK+LY L + ++ID+ +K ++E+ SGK Y+LS+R L+I W D R YW+W D
Sbjct: 63 SSKKELYRCLCES-ILIDNGRKIFKIEKLSGKISYILSSRDLSITWSDQRHYWSWSPRSD 121
Query: 126 SRLA 129
SR +
Sbjct: 122 SRFS 125
>At1g56250 hypothetical protein
Length = 282
Score = 103 bits (257), Expect = 2e-23
Identities = 56/124 (45%), Positives = 78/124 (62%), Gaps = 2/124 (1%)
Query: 7 LPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPS-LTNI 65
LPE CIA I++ T+P DA S S FR A DSD VW +FLPSD S+IS S NI
Sbjct: 4 LPEACIANILAFTSPADAFSSSEVSSVFRLAGDSDFVWEKFLPSDYKSLISQSTDHHWNI 63
Query: 66 PTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPD 125
+KK++Y L D ++ID+ +K ++ + SGK Y+LSAR ++I D YW+W ++ D
Sbjct: 64 SSKKEIYRCLCD-SLLIDNARKLFKINKFSGKISYVLSARDISITHSDHASYWSWSNVSD 122
Query: 126 SRLA 129
SR +
Sbjct: 123 SRFS 126
>At2g02310 putative phloem-specific lectin
Length = 307
Score = 101 bits (251), Expect = 1e-22
Identities = 52/121 (42%), Positives = 73/121 (59%), Gaps = 3/121 (2%)
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNI 65
DLPE CI+ I+S T+P D + SK+F A D++W +FLPS+ S+I P
Sbjct: 47 DLPEDCISNIISFTSPRDVCVSASVSKSFAHAVQCDSIWEKFLPSEYESLI---PPWRVF 103
Query: 66 PTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPD 125
+KKDLY L PV+++ KKS LE SGKKC +L+A+ L I G++ YW WI + +
Sbjct: 104 SSKKDLYFTLCYDPVLVEDGKKSFWLETASGKKCVLLAAKELWITGGNNPEYWQWIELCE 163
Query: 126 S 126
S
Sbjct: 164 S 164
>At2g02320 unknown protein
Length = 307
Score = 97.4 bits (241), Expect = 2e-21
Identities = 51/121 (42%), Positives = 75/121 (61%), Gaps = 6/121 (4%)
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNI 65
DLPE CI+ I+S T+P DA ++ SKTF SA SD VW +F+P + S++S S +
Sbjct: 42 DLPEECISLIISFTSPRDACVFALVSKTFESAVQSDIVWEKFIPPEYESLLSRS---QHF 98
Query: 66 PTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPD 125
+KK+L+ AL D V+I+ KK + +E+ +GK+C MLSA +L + W WI+ P
Sbjct: 99 SSKKELFFALCDESVLINVSKKDLWIEKATGKRCMMLSASALNL---STHHTWKWITNPV 155
Query: 126 S 126
S
Sbjct: 156 S 156
>At2g02350 SKP1 interacting partner 3 (SKIP3)
Length = 294
Score = 96.3 bits (238), Expect = 4e-21
Identities = 53/125 (42%), Positives = 79/125 (62%), Gaps = 4/125 (3%)
Query: 4 IQDLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLT 63
+ LPEGCI+ I+S T+P DA + SK F SA SD VW +FLP+D S+I+ S +
Sbjct: 24 LDSLPEGCISNIISFTSPEDACVAAAVSKIFESAVKSDIVWEKFLPTDYESLITPSRVFS 83
Query: 64 NIPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAI-VWGDDRRYWNWIS 122
+KK+LY +L + P++I+ K S+ LE+ SGK+C MLSA ++ + D + + WI
Sbjct: 84 ---SKKELYFSLCNDPLLIEDGKMSLWLEKASGKRCIMLSATAMNLSSMADMSQRFLWIP 140
Query: 123 MPDSR 127
P+SR
Sbjct: 141 CPESR 145
>At1g56240 hypothetical protein
Length = 284
Score = 94.7 bits (234), Expect = 1e-20
Identities = 52/124 (41%), Positives = 76/124 (60%), Gaps = 2/124 (1%)
Query: 7 LPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNI- 65
LPE C+A I++ T+P DA S S FR A DSD VW +FLPS S+IS S I
Sbjct: 4 LPEACVANILAFTSPADAFSSSEVSSVFRLAGDSDFVWEKFLPSHYKSLISQSTDHHRIF 63
Query: 66 PTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPD 125
+KK++Y L D ++ID+ +K ++ + SGK Y+LSAR ++I + D Y +W ++ D
Sbjct: 64 SSKKEIYRCLCD-SLLIDNARKLFKINKFSGKISYILSARDISITYSDHASYCSWSNVSD 122
Query: 126 SRLA 129
SR +
Sbjct: 123 SRFS 126
>At3g53000 unknown protein
Length = 300
Score = 94.4 bits (233), Expect = 1e-20
Identities = 45/123 (36%), Positives = 74/123 (59%), Gaps = 2/123 (1%)
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISH-SPSLTN 64
D+PE C+A + TP + L+ +++FR A+ SD+VW + LP + ++ P +
Sbjct: 23 DIPESCVACVFMYLTPPEICNLAGLNRSFRGAASSDSVWEKKLPENYQDLLDLLPPERYH 82
Query: 65 IPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMP 124
+KKD++ LS RP+ D D K + ++R +G+ C +SAR ++I +DRRYWNWI
Sbjct: 83 SLSKKDIFAVLS-RPIPFDDDNKEVWIDRVTGRVCMAISARGMSITGIEDRRYWNWIPTE 141
Query: 125 DSR 127
+SR
Sbjct: 142 ESR 144
>At2g02300 putative phloem-specific lectin
Length = 284
Score = 90.5 bits (223), Expect = 2e-19
Identities = 54/123 (43%), Positives = 74/123 (59%), Gaps = 4/123 (3%)
Query: 6 DLPEGCIAAIVSRT-TPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTN 64
DLP+ C+A I S T TP DA ++ SK+F +SD+VW +FLP +S P
Sbjct: 36 DLPDDCLAIISSFTSTPRDAFLAALVSKSFGLQFNSDSVWEKFLPPP--DYVSLLPKSRV 93
Query: 65 IPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMP 124
+KK+LY AL D P + K S +L++ SGKKC MLSA+ L I + +YW WIS+P
Sbjct: 94 FSSKKELYFALCD-PFPNHNGKMSFRLDKASGKKCVMLSAKKLLISRVVNPKYWKWISIP 152
Query: 125 DSR 127
+SR
Sbjct: 153 ESR 155
>At2g02270 putative phloem-specific lectin
Length = 265
Score = 87.8 bits (216), Expect = 1e-18
Identities = 47/105 (44%), Positives = 65/105 (61%), Gaps = 3/105 (2%)
Query: 7 LPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNIP 66
LPE CI+ I+S TTP DA + SK F SA SD+VW +FLP D SS++ P
Sbjct: 16 LPENCISNIISFTTPRDACFAASVSKAFESAVQSDSVWEKFLPLDYSSLV---PESRVFL 72
Query: 67 TKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVW 111
+KK+L +L P++I+ KKS L++ SG+KC MLS + + I W
Sbjct: 73 SKKELCFSLCRVPLLIEGGKKSFWLDKTSGEKCIMLSPKGMVISW 117
>At3g61060 unknown protein
Length = 290
Score = 84.0 bits (206), Expect = 2e-17
Identities = 47/126 (37%), Positives = 76/126 (60%), Gaps = 7/126 (5%)
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSP----S 61
DLPE C+A I++R P + RL+ ++ FR AS +D +W LP++ +I+H +
Sbjct: 27 DLPENCVALIMTRLDPPEICRLARLNRMFRRASSADFIWESKLPANY-RVIAHKVFDEIT 85
Query: 62 LTNIPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWI 121
LT + KKDLY LS +P + D K + +++ +G+ C +S+++L I DDRRYW+ I
Sbjct: 86 LTKL-IKKDLYAKLS-QPNLFDDGTKELWIDKNTGRLCLSISSKALRITGIDDRRYWSHI 143
Query: 122 SMPDSR 127
+SR
Sbjct: 144 PTDESR 149
>At1g63090 unknown protein
Length = 289
Score = 75.1 bits (183), Expect = 9e-15
Identities = 45/125 (36%), Positives = 64/125 (51%), Gaps = 2/125 (1%)
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISH-SPSLTN 64
DLPE C+A I+ P++ R S + F AS +D VW LP D I+ S +
Sbjct: 28 DLPESCVALILQNLDPVEICRFSKLNTAFHGASWADFVWESKLPPDYKLILEKILGSFPD 87
Query: 65 IPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMP 124
K+D++ LS R D K +++++G C SA+ L+I DDRRYW+ I
Sbjct: 88 NLRKRDIFTFLS-RVNSFDEGNKKAWVDKRTGGLCLCTSAKGLSITGIDDRRYWSHIPSD 146
Query: 125 DSRLA 129
DSR A
Sbjct: 147 DSRFA 151
>At1g12710 unknown protein
Length = 291
Score = 73.9 bits (180), Expect = 2e-14
Identities = 44/127 (34%), Positives = 66/127 (51%), Gaps = 6/127 (4%)
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNI 65
DLPE C+A IV P++ R S ++ FR AS +D VW LP + ++ L
Sbjct: 30 DLPEACVAIIVENLDPVEICRFSKLNRAFRGASWADCVWESKLPQNYRDVL--EKILGGF 87
Query: 66 P---TKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWIS 122
P K+ LY LS R D K + +++++ C +SA+ L+I DDRRYW+ I
Sbjct: 88 PENLQKRHLYAFLS-RINSFDDATKKVWIDKRTSGVCLSISAKGLSITGIDDRRYWSHIP 146
Query: 123 MPDSRLA 129
+SR +
Sbjct: 147 TDESRFS 153
>At5g52120 unknown protein
Length = 291
Score = 71.6 bits (174), Expect = 1e-13
Identities = 45/132 (34%), Positives = 67/132 (50%), Gaps = 9/132 (6%)
Query: 4 IQDLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISH----- 58
++D+PE CI A+ P + L+ +K+F AS SDAVW LPS+ ++
Sbjct: 21 LEDVPENCITAMFMYMEPPEICLLARVNKSFHRASRSDAVWEDKLPSNYKFLVRRILEDQ 80
Query: 59 ---SPSLTNIPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDR 115
I KK++Y L RP + D K L+++SGK +S +++ I DDR
Sbjct: 81 QQVGVKDKLIYRKKEIYARLC-RPNLFDTGTKEAWLDKRSGKVFLAISPKAMKITGIDDR 139
Query: 116 RYWNWISMPDSR 127
RYW IS +SR
Sbjct: 140 RYWEHISSDESR 151
>At1g80110 unknown protein
Length = 163
Score = 45.4 bits (106), Expect = 8e-06
Identities = 16/27 (59%), Positives = 25/27 (92%)
Query: 101 MLSARSLAIVWGDDRRYWNWISMPDSR 127
M++AR+L IVWG ++RYW+WIS+P++R
Sbjct: 1 MMAARALNIVWGHEQRYWHWISLPNTR 27
>At4g19840 lectin like protein
Length = 246
Score = 38.9 bits (89), Expect = 7e-04
Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 2/35 (5%)
Query: 92 ERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPDS 126
ER S C+ML A++L+I W DD YW W + +S
Sbjct: 89 ERNSN--CFMLFAKNLSITWSDDVNYWTWFTEKES 121
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.132 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,863,257
Number of Sequences: 26719
Number of extensions: 108616
Number of successful extensions: 434
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 379
Number of HSP's gapped (non-prelim): 43
length of query: 130
length of database: 11,318,596
effective HSP length: 88
effective length of query: 42
effective length of database: 8,967,324
effective search space: 376627608
effective search space used: 376627608
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146559.3