BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146554.10 + phase: 2 /partial
(83 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g31030 F17F8.5 40 2e-04
At1g23990 reverse transcriptase, putative 39 5e-04
At2g23880 putative non-LTR retroelement reverse transcriptase 38 0.001
At1g19030 hypothetical protein 38 0.001
At3g42890 putative protein 37 0.002
At2g02520 putative non-LTR retroelement reverse transcriptase 37 0.002
At2g17910 putative non-LTR retroelement reverse transcriptase 36 0.004
At3g26855 putative protein 35 0.007
At1g32390 hypothetical protein 35 0.007
At2g02650 putative reverse transcriptase 34 0.011
At1g21330 hypothetical protein 34 0.011
At1g51750 hypothetical protein 34 0.015
At5g18880 SAE1-S9-protein - like 33 0.025
At2g17610 putative non-LTR retroelement reverse transcriptase 33 0.025
At3g28160 non-LTR reverse transcriptase, putative 33 0.033
At4g09710 RNA-directed DNA polymerase -like protein 32 0.043
At3g31420 hypothetical protein 32 0.043
At1g10160 putative reverse transcriptase 32 0.056
At4g02490 32 0.073
At1g43730 hypothetical protein 32 0.073
>At1g31030 F17F8.5
Length = 872
Score = 40.4 bits (93), Expect = 2e-04
Identities = 19/61 (31%), Positives = 33/61 (53%), Gaps = 6/61 (9%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLV---ARGTLAPDAQLCVSGCWAVETAQHLFVS 77
+W RH K ++ W + NRLPT ++ + G+++ + LC + +T +HLF S
Sbjct: 707 VWFRHATPKYALCTWLAIHNRLPTGDRMLKWNSSGSVSGNCVLCTNNS---KTLEHLFFS 763
Query: 78 C 78
C
Sbjct: 764 C 764
>At1g23990 reverse transcriptase, putative
Length = 657
Score = 38.9 bits (89), Expect = 5e-04
Identities = 22/59 (37%), Positives = 29/59 (48%), Gaps = 4/59 (6%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVA-RGTLAPDAQLCVSGCWAVETAQHLFVSC 78
IW H K S AW ++NRL T ++ L+P LC + +ET HLF SC
Sbjct: 494 IWFAHATPKFSFCAWLAVQNRLSTGDKMLQWNRRLSPTCVLCNNN---IETRNHLFFSC 549
>At2g23880 putative non-LTR retroelement reverse transcriptase
Length = 1216
Score = 37.7 bits (86), Expect = 0.001
Identities = 20/58 (34%), Positives = 25/58 (42%), Gaps = 2/58 (3%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETAQHLFVSC 78
+W H K S AW +RNRL T ++ P CV +ET HLF C
Sbjct: 782 VWFAHATPKFSFCAWLAIRNRLSTGDRMMTWNNGTPTT--CVFCSSPMETRDHLFFQC 837
>At1g19030 hypothetical protein
Length = 398
Score = 37.7 bits (86), Expect = 0.001
Identities = 20/60 (33%), Positives = 30/60 (49%), Gaps = 4/60 (6%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVA-RGTLAPDAQLCVSGCWAVETAQHLFVSCP 79
IW +H K S++ W + +NR+ T L+ + P+ LC +ET HLF CP
Sbjct: 289 IWFKHSTPKYSLITWLVQKNRVATGDKLIKWNPQVTPNCILCQH---TLETWDHLFFMCP 345
>At3g42890 putative protein
Length = 534
Score = 37.0 bits (84), Expect = 0.002
Identities = 23/60 (38%), Positives = 29/60 (48%), Gaps = 4/60 (6%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGC-WAVETAQHLFVSCP 79
+W + K S + W RNRL T LV DA S C A+ET +H+F SCP
Sbjct: 108 VWFPNSTPKYSFITWIAFRNRLATGEKLVKWNN---DANGGCSFCDEAMETREHIFFSCP 164
>At2g02520 putative non-LTR retroelement reverse transcriptase
Length = 211
Score = 37.0 bits (84), Expect = 0.002
Identities = 21/58 (36%), Positives = 31/58 (53%), Gaps = 2/58 (3%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETAQHLFVSC 78
IW + K + +AW +R+RL TK +++ G + P LC+ ET QHLF C
Sbjct: 47 IWFKGKIPKHAFIAWVNMRHRLHTKDRMISWGFIFP--PLCLFCNTHDETRQHLFFDC 102
>At2g17910 putative non-LTR retroelement reverse transcriptase
Length = 1344
Score = 35.8 bits (81), Expect = 0.004
Identities = 18/60 (30%), Positives = 26/60 (43%), Gaps = 2/60 (3%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETAQHLFVSCPI 80
IW+ H K+ I W L +P + L RG + D C+ ET H+ CP+
Sbjct: 1024 IWNLHTAPKIRIFLWKALHGAIPVEDRLRTRGIRSDDG--CLMCDTENETINHILFECPL 1081
>At3g26855 putative protein
Length = 163
Score = 35.0 bits (79), Expect = 0.007
Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 3/59 (5%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETAQHLFVSCP 79
IW + K+ +L W L N LP A L++R + + C ET H+ +CP
Sbjct: 9 IWSLKISPKIKLLIWKALNNALPVGAQLLSRNI---SIEPFCTRCRDFETITHILFNCP 64
>At1g32390 hypothetical protein
Length = 192
Score = 35.0 bits (79), Expect = 0.007
Identities = 19/58 (32%), Positives = 24/58 (40%), Gaps = 2/58 (3%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETAQHLFVSC 78
+W H K S AW +RNRL ++ P CV +ET HLF C
Sbjct: 69 VWFAHATPKFSFCAWLAVRNRLSMVDRMMTWNNGTPTT--CVFCSSPMETRDHLFFQC 124
>At2g02650 putative reverse transcriptase
Length = 365
Score = 34.3 bits (77), Expect = 0.011
Identities = 19/63 (30%), Positives = 26/63 (41%), Gaps = 2/63 (3%)
Query: 17 VSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETAQHLFV 76
V + IW HV K+ W + L T L +R A +C C ET H+
Sbjct: 33 VKQAIWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDAD--PICQRCCIEEETIHHIMF 90
Query: 77 SCP 79
+CP
Sbjct: 91 NCP 93
>At1g21330 hypothetical protein
Length = 211
Score = 34.3 bits (77), Expect = 0.011
Identities = 21/64 (32%), Positives = 27/64 (41%), Gaps = 4/64 (6%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWA-VETAQHLFVSCP 79
+W H K S + W +RNRL T ++ C C A VET HLF +C
Sbjct: 48 VWLNHHKPKFSFMTWLAVRNRLSTGDRMLQWNM---GVNSCCVFCHAPVETRNHLFFACQ 104
Query: 80 IFGD 83
D
Sbjct: 105 YSAD 108
>At1g51750 hypothetical protein
Length = 629
Score = 33.9 bits (76), Expect = 0.015
Identities = 23/69 (33%), Positives = 32/69 (46%), Gaps = 6/69 (8%)
Query: 12 NTDVAVSELIWHRHVPLKVSILAWCLLRNRLPT--KANLVARGTLAPDAQLCVSGCWAVE 69
+ +VA + +W H K W LRNRL T + L G+ D + C ++E
Sbjct: 457 SNEVAWYKGVWFSHSTPKYQFCTWLALRNRLSTGYRMQLWNNGS---DVK-CTFCSTSIE 512
Query: 70 TAQHLFVSC 78
T HLF SC
Sbjct: 513 TRDHLFFSC 521
>At5g18880 SAE1-S9-protein - like
Length = 295
Score = 33.1 bits (74), Expect = 0.025
Identities = 19/62 (30%), Positives = 30/62 (47%), Gaps = 4/62 (6%)
Query: 18 SELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDA-QLCVSGCWAVETAQHLFV 76
++++W + + S++ W RLPT+ L G P + LC +G ET HLF
Sbjct: 128 AKVVWFKEYIPRFSLITWMSFLERLPTRDRLRGWGMNIPSSWVLCSNG---DETHAHLFF 184
Query: 77 SC 78
C
Sbjct: 185 EC 186
>At2g17610 putative non-LTR retroelement reverse transcriptase
Length = 773
Score = 33.1 bits (74), Expect = 0.025
Identities = 19/60 (31%), Positives = 24/60 (39%), Gaps = 2/60 (3%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETAQHLFVSCPI 80
IW ++ P K+ W N LPT NL R + D C A E HL C +
Sbjct: 501 IWKQNAPPKIKHFWWRSAHNALPTAGNLKRRRLITDDT--CQRCGEASEDVNHLLFQCRV 558
>At3g28160 non-LTR reverse transcriptase, putative
Length = 630
Score = 32.7 bits (73), Expect = 0.033
Identities = 23/64 (35%), Positives = 26/64 (39%), Gaps = 12/64 (18%)
Query: 21 IWHRHVPLKVSILAWCLLRNRLPT-----KANLVARGTLAPDAQLCVSGCWAVETAQHLF 75
+W K S + W NRL T K N AR T CV +ET HLF
Sbjct: 283 VWFPSSTPKYSFVTWLAFHNRLATGDRLYKWNSEARAT-------CVFCDEELETRDHLF 335
Query: 76 VSCP 79
SCP
Sbjct: 336 FSCP 339
>At4g09710 RNA-directed DNA polymerase -like protein
Length = 1274
Score = 32.3 bits (72), Expect = 0.043
Identities = 22/82 (26%), Positives = 33/82 (39%), Gaps = 6/82 (7%)
Query: 1 TGYDLLTADD---NNTDVAVSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPD 57
TGY L + + D + IW H KV W ++ LP L R +
Sbjct: 951 TGYALAKLNSFPASQLDFNWQKNIWKIHTSPKVKHFLWKAMKGALPVGEALSRRNI---E 1007
Query: 58 AQLCVSGCWAVETAQHLFVSCP 79
A++ C E++ HL + CP
Sbjct: 1008 AEVTCKRCGQTESSLHLMLLCP 1029
>At3g31420 hypothetical protein
Length = 1491
Score = 32.3 bits (72), Expect = 0.043
Identities = 18/65 (27%), Positives = 27/65 (40%), Gaps = 2/65 (3%)
Query: 14 DVAVSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETAQH 73
D+ + +L+W K+ W +L + T L R +C C ET+QH
Sbjct: 1161 DIKLKQLLWKTKTAPKIKHFCWKILSGAIATGEMLRYRHI--NKQSICKRCCRDEETSQH 1218
Query: 74 LFVSC 78
LF C
Sbjct: 1219 LFFEC 1223
>At1g10160 putative reverse transcriptase
Length = 1118
Score = 32.0 bits (71), Expect = 0.056
Identities = 22/70 (31%), Positives = 35/70 (49%), Gaps = 6/70 (8%)
Query: 11 NNTDVAVSELIWHR-HVPLKVSILAWCLLRNRLPTKANLVARG-TLAPDAQLCVSGCWAV 68
++T V + +W + HVP K + + W + NRL T+ L G ++ P LC
Sbjct: 937 SSTSVLWHKAVWFKDHVP-KQAFICWVVAHNRLHTRDRLRRWGFSIPPTCVLCND---LD 992
Query: 69 ETAQHLFVSC 78
E+ +HLF C
Sbjct: 993 ESREHLFFRC 1002
>At4g02490
Length = 657
Score = 31.6 bits (70), Expect = 0.073
Identities = 19/67 (28%), Positives = 31/67 (45%), Gaps = 2/67 (2%)
Query: 12 NTDVAVSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETA 71
+ V+ + +W + K + ++W NRL T+ L + G + P CV ET
Sbjct: 577 SVSVSWHKAVWFTNQVPKHAFISWVTAWNRLHTRDRLRSWGLIVPAE--CVLCNLVDETR 634
Query: 72 QHLFVSC 78
HLF +C
Sbjct: 635 DHLFFAC 641
>At1g43730 hypothetical protein
Length = 320
Score = 31.6 bits (70), Expect = 0.073
Identities = 21/63 (33%), Positives = 31/63 (48%), Gaps = 4/63 (6%)
Query: 21 IWHR-HVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSGCWAVETAQHLFVSCP 79
+W + HVP K + + W + NRL T+ L + G P +C+ E+ HLF CP
Sbjct: 155 VWFKNHVP-KHAFICWVVAWNRLHTRDRLRSWGLSIP--AVCLLCNSHDESRAHLFFECP 211
Query: 80 IFG 82
G
Sbjct: 212 FCG 214
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.324 0.137 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,847,816
Number of Sequences: 26719
Number of extensions: 57456
Number of successful extensions: 199
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 30
Number of HSP's that attempted gapping in prelim test: 159
Number of HSP's gapped (non-prelim): 56
length of query: 83
length of database: 11,318,596
effective HSP length: 59
effective length of query: 24
effective length of database: 9,742,175
effective search space: 233812200
effective search space used: 233812200
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146554.10