BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146548.2 + phase: 0
(203 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g19860 putative bHLH transcription factor (bHLH121) 93 1e-19
At4g36060 transcription factor BHLH11 like protein 91 3e-19
At5g54680 putative bHLH transcription factor (bHLH105) 72 2e-13
At3g23210 putative bHLH transcription factor (bHLH034) 65 2e-11
At4g14410 putative bHLH transcription factor (bHLH104) 64 5e-11
At1g51070 bHLH transcription factor (bHLH115) like protein 58 4e-09
At5g46830 putative transcription factor bHLH28 45 3e-05
At2g46510 putative bHLH transcription factor 40 0.001
At5g39870 putative protein 39 0.002
At4g34590 bZIP transcription factor ATB2/ Atbzip11 39 0.002
At1g61080 hypothetical protein 39 0.002
At5g62410 chromosomal protein - like 37 0.007
At4g36730 G-box binding bZip transcription factor GBF1 / AtbZip41 37 0.009
At1g50230 hypothetical protein 35 0.021
At1g63650 putative transcription factor (BHLH2) 35 0.027
At1g26150 Pto kinase interactor, putative 35 0.027
At5g10570 putative bHLH transcription factor (bHLH061) 35 0.036
At3g22120 unknown protein 35 0.036
At1g70460 putative protein kinase 34 0.046
At1g06850 bZip transcription factor AtbZip52 34 0.046
>At3g19860 putative bHLH transcription factor (bHLH121)
Length = 284
Score = 92.8 bits (229), Expect = 1e-19
Identities = 62/200 (31%), Positives = 92/200 (46%), Gaps = 31/200 (15%)
Query: 8 KRIHKAEREKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQIQSLKKE 67
++ KA REK++RE LNE F++L N LD P N KA+IL + +LLK+L ++ LK E
Sbjct: 6 RKSQKAGREKLRREKLNEHFVELGNVLDPERPKNDKATILTDTVQLLKELTSEVNKLKSE 65
Query: 68 NVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKP--------DLNAPP- 118
+L ES +T EKN+L+EE +SL++ IE L + Q R+ P + APP
Sbjct: 66 YTALTDESRELTQEKNDLREEKTSLKSDIENLNLQYQQRLRSMSPWGAAMDHTVMMAPPP 125
Query: 119 ---------------QLELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAY 163
+ P T F Q +P++ P +P+ P
Sbjct: 126 SFPYPMPIAMPPGSIPMHPSMPSYTYFGNQ-------NPSMIPAPCPTYMPYMPPNTVVE 178
Query: 164 PAPTEITPNPPLVISKPHAR 183
I NP +P A+
Sbjct: 179 QQSVHIPQNPGNRSREPRAK 198
>At4g36060 transcription factor BHLH11 like protein
Length = 286
Score = 91.3 bits (225), Expect = 3e-19
Identities = 55/142 (38%), Positives = 83/142 (57%), Gaps = 6/142 (4%)
Query: 12 KAEREKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQIQSLKKENVSL 71
KAEREK++R+ L E FL+L NALD + P + KAS+L + ++LKD++ Q+ LK E +L
Sbjct: 49 KAEREKLRRDKLKEQFLELGNALDPNRPKSDKASVLTDTIQMLKDVMNQVDRLKAEYETL 108
Query: 72 LSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQLELESPEQTTFS 131
ES + EK+EL+EE ++L++ IE L + Q RI P + P P
Sbjct: 109 SQESRELIQEKSELREEKATLKSDIEILNAQYQHRIKTMVPWV---PHYSYHIPFVAITQ 165
Query: 132 GQSFQLP---TVDPTLQQGPTV 150
GQS +P +V+P +Q +V
Sbjct: 166 GQSSFIPYSASVNPLTEQQASV 187
>At5g54680 putative bHLH transcription factor (bHLH105)
Length = 234
Score = 72.0 bits (175), Expect = 2e-13
Identities = 45/128 (35%), Positives = 69/128 (53%), Gaps = 7/128 (5%)
Query: 12 KAEREKMKREHLNELFLDLANALDLSEP-NNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA REK +R+ LN+ F++L L+ P KA+IL++A R++ L + Q LK N S
Sbjct: 76 KACREKQRRDRLNDKFMELGAILEPGNPPKTDKAAILVDAVRMVTQLRGEAQKLKDSNSS 135
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQLELESPEQTTF 130
L + + EKNEL++E L+T+ EKL+ +++A A APP + T F
Sbjct: 136 LQDKIKELKTEKNELRDEKQRLKTEKEKLEQQLKAMNAPQPSFFPAPPMM------PTAF 189
Query: 131 SGQSFQLP 138
+ Q P
Sbjct: 190 ASAQGQAP 197
>At3g23210 putative bHLH transcription factor (bHLH034)
Length = 291
Score = 65.5 bits (158), Expect = 2e-11
Identities = 37/110 (33%), Positives = 65/110 (58%), Gaps = 1/110 (0%)
Query: 2 KQGKVPKRIHKAEREKMKREHLNELFLDLANALDLSE-PNNGKASILIEASRLLKDLLCQ 60
+ G K KA REK++RE LN+ F+DL++ L+ P K++IL +A R++ L +
Sbjct: 128 RTGSCSKPGTKACREKLRREKLNDKFMDLSSVLEPGRTPKTDKSAILDDAIRVVNQLRGE 187
Query: 61 IQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQS 110
L++ N LL E + +KNEL+EE L+ + EK++ ++++ + S
Sbjct: 188 AHELQETNQKLLEEIKSLKADKNELREEKLVLKAEKEKMEQQLKSMVVPS 237
>At4g14410 putative bHLH transcription factor (bHLH104)
Length = 283
Score = 63.9 bits (154), Expect = 5e-11
Identities = 38/100 (38%), Positives = 59/100 (59%), Gaps = 1/100 (1%)
Query: 12 KAEREKMKREHLNELFLDLANALDLSE-PNNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA RE+++RE LNE F+DL++ L+ P K +IL +A R+L L + L++ N
Sbjct: 135 KACRERLRREKLNERFMDLSSVLEPGRTPKTDKPAILDDAIRILNQLRDEALKLEETNQK 194
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQS 110
LL E + EKNEL+EE L+ EK + ++++ A S
Sbjct: 195 LLEEIKSLKAEKNELREEKLVLKADKEKTEQQLKSMTAPS 234
>At1g51070 bHLH transcription factor (bHLH115) like protein
Length = 226
Score = 57.8 bits (138), Expect = 4e-09
Identities = 33/102 (32%), Positives = 58/102 (56%), Gaps = 1/102 (0%)
Query: 12 KAEREKMKREHLNELFLDLANALDLSE-PNNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA REK +R+ LN+ F +L++ L+ P K +I+ +A R++ + Q LK N S
Sbjct: 71 KACREKQRRDRLNDKFTELSSVLEPGRTPKTDKVAIINDAIRMVNQARDEAQKLKDLNSS 130
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKP 112
L + + EKNEL++E L+ + E++ +++A Q +P
Sbjct: 131 LQEKIKELKDEKNELRDEKQKLKVEKERIDQQLKAIKTQPQP 172
>At5g46830 putative transcription factor bHLH28
Length = 511
Score = 44.7 bits (104), Expect = 3e-05
Identities = 31/91 (34%), Positives = 48/91 (52%), Gaps = 13/91 (14%)
Query: 2 KQGKVP-----KRIHKAEREKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKD 56
K+G+ P K ++ E E+M+RE LN F AL PN K ++ + LL+D
Sbjct: 329 KRGRKPAHGRDKPLNHVEAERMRREKLNHRFY----ALRAVVPNVSK----MDKTSLLED 380
Query: 57 LLCQIQSLKKENVSLLSESHYVTMEKNELKE 87
+C I LK + ++ E H + ++ NELKE
Sbjct: 381 AVCYINELKSKAENVELEKHAIEIQFNELKE 411
>At2g46510 putative bHLH transcription factor
Length = 662
Score = 39.7 bits (91), Expect = 0.001
Identities = 47/203 (23%), Positives = 98/203 (48%), Gaps = 19/203 (9%)
Query: 2 KQGKVPKR-----IHKAEREKMKREHLNELFLDLANAL-DLSEPNNGKASILIEASRLLK 55
K+G+ P ++ E E+ +RE LN+ F L + + ++S+ + KAS+L +A +K
Sbjct: 381 KRGRKPANGREEPLNHVEAERQRREKLNQRFYALRSVVPNISKMD--KASLLGDAISYIK 438
Query: 56 DLLCQIQSLKKENVSL---LSESHYVTMEKN---ELKEENSSLETQ-IEKLQGEIQARIA 108
+L +++ ++ E V LSES+ +T+E++ +++ N + + I L +RI
Sbjct: 439 ELQEKVKIMEDERVGTDKSLSESNTITVEESPEVDIQAMNEEVVVRVISPLDSHPASRII 498
Query: 109 QSKPDLNAP-PQLELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPT 167
Q+ + N + +L E T F +F + + + + L+ F P+ + P
Sbjct: 499 QAMRNSNVSLMEAKLSLAEDTMF--HTFVIKSNNGSDPLTKEKLIAAFYPETSSTQPPLP 556
Query: 168 EITPNPPLVISKPHARY-PTPAD 189
+ +S + + P+P+D
Sbjct: 557 SSSSQVSGFVSPIRSNFNPSPSD 579
>At5g39870 putative protein
Length = 290
Score = 38.9 bits (89), Expect = 0.002
Identities = 37/159 (23%), Positives = 68/159 (42%), Gaps = 13/159 (8%)
Query: 2 KQGKVPKRIHKAEREKMKREHLNELFLD-LANALDLSEPNNGKASILIEASRLLKDLLCQ 60
K+G V K + + + E M +L + + +A NG A + +E + +KD + +
Sbjct: 119 KEGSVNKSLQREKMEAMNTINLLQSVGEKIAGGRSNKTETNGVAKLTVEQQKEMKDGILK 178
Query: 61 ----IQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARI--------A 108
I + K NV + +S + E KEE SS +TQI++ A+I
Sbjct: 179 WLQVITRIVKTNVEINLKSSSKSQTTQESKEEKSSTQTQIKRRSEREYAQITALPRGSRV 238
Query: 109 QSKPDLNAPPQLELESPEQTTFSGQSFQLPTVDPTLQQG 147
+K ++N + SP++ G + P ++G
Sbjct: 239 TNKGNINGILKSARNSPKEIDDHGNKRRKPKKQYKFKEG 277
>At4g34590 bZIP transcription factor ATB2/ Atbzip11
Length = 159
Score = 38.5 bits (88), Expect = 0.002
Identities = 18/58 (31%), Positives = 32/58 (55%)
Query: 48 IEASRLLKDLLCQIQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQA 105
++ +LL DL Q+ LKKEN +++ T ++ ENS L Q+++L +Q+
Sbjct: 44 MKKQKLLDDLTAQVNHLKKENTEIVTSVSITTQHYLTVEAENSVLRAQLDELNHRLQS 101
>At1g61080 hypothetical protein
Length = 907
Score = 38.5 bits (88), Expect = 0.002
Identities = 40/177 (22%), Positives = 68/177 (37%), Gaps = 18/177 (10%)
Query: 16 EKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQIQSLK-----KENVS 70
EK+ + L + + + + EPN +I +K +I+S+ +E+V+
Sbjct: 314 EKLSPIDVKRLAIHILSQKEAQEPNESNDEDVISVVEEIKQKKDEIESIDVKMETEESVN 373
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQLELESPEQTTF 130
L ES + E++ + + +S T KL + ++ L PP P +
Sbjct: 374 LDEESVVLNGEQDTIMKISSLESTSESKLN---HSEKYENSSQLFPPPPPPPPPPPLSFI 430
Query: 131 SGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVISKPHARYPTP 187
S LP+ PT P D+ + P P P PP V+ H P P
Sbjct: 431 KTASLPLPSPPPT----------PPIADIAISMPPPPPPPPPPPAVMPLKHFAPPPP 477
>At5g62410 chromosomal protein - like
Length = 1175
Score = 37.0 bits (84), Expect = 0.007
Identities = 22/101 (21%), Positives = 53/101 (51%), Gaps = 2/101 (1%)
Query: 5 KVPKRIHKAEREKMKREHLNELFLDLANALD--LSEPNNGKASILIEASRLLKDLLCQIQ 62
K+ + + +A+ + ++E + D + L+ + + + + L + + +K + Q+Q
Sbjct: 745 KLEEELEEAKSQIKEKELAYKNCFDAVSKLENSIKDHDKNREGRLKDLEKNIKTIKAQMQ 804
Query: 63 SLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEI 103
+ K+ S +E + ME+ +K+E SSLE+ + L+ +I
Sbjct: 805 AASKDLKSHENEKEKLVMEEEAMKQEQSSLESHLTSLETQI 845
>At4g36730 G-box binding bZip transcription factor GBF1 / AtbZip41
Length = 315
Score = 36.6 bits (83), Expect = 0.009
Identities = 22/66 (33%), Positives = 39/66 (58%), Gaps = 6/66 (9%)
Query: 51 SRLLKDLLCQ-----IQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQG-EIQ 104
SRL K C+ ++SL EN SL E ++ E ++LK EN+S++ +++++ G E
Sbjct: 239 SRLRKQAECEQLQQRVESLSNENQSLRDELQRLSSECDKLKSENNSIQDELQRVLGAEAV 298
Query: 105 ARIAQS 110
A + Q+
Sbjct: 299 ANLEQN 304
>At1g50230 hypothetical protein
Length = 269
Score = 35.4 bits (80), Expect = 0.021
Identities = 27/97 (27%), Positives = 48/97 (48%), Gaps = 10/97 (10%)
Query: 1 MKQGKVPKRIHKAERE-----KMKREHLNELFLDLANALD---LSEPNNGKASILIEASR 52
MKQGK K IH +E K+K E++ E+ NA + ++E G+ ++E +
Sbjct: 38 MKQGKTDKDIHSLRQEIEILRKLKHENIIEMLDSFENAREFCVVTEFAQGELFEILEDDK 97
Query: 53 LLKDLLCQIQSLKKENVSLLSESHYVTMEKNELKEEN 89
L + Q+Q++ K+ V L H + ++K +N
Sbjct: 98 CLPEE--QVQAIAKQLVKALDYLHSNRIIHRDMKPQN 132
>At1g63650 putative transcription factor (BHLH2)
Length = 596
Score = 35.0 bits (79), Expect = 0.027
Identities = 29/99 (29%), Positives = 48/99 (48%), Gaps = 4/99 (4%)
Query: 16 EKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQIQSLKKENVSLLSES 75
EK +RE LNE F+ L + + S K SIL + L+DL ++Q L+ S +E+
Sbjct: 410 EKKRREKLNERFMTLRSIIP-SISKIDKVSILDDTIEYLQDLQKRVQELESCRESADTET 468
Query: 76 HYVTM--EKNELKEENSSLETQIEKLQGEIQARIAQSKP 112
M +K + +EE +S K +G + + +P
Sbjct: 469 RITMMKRKKPDDEEERASANCMNSKRKGS-DVNVGEDEP 506
>At1g26150 Pto kinase interactor, putative
Length = 760
Score = 35.0 bits (79), Expect = 0.027
Identities = 28/99 (28%), Positives = 36/99 (36%), Gaps = 9/99 (9%)
Query: 110 SKPDLNAPPQLELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRP------DLQAAY 163
S P +PP L P TT P+ P L PT P P +
Sbjct: 70 SPPPEPSPPSPSLTGPPPTTIPVSPPPEPSPPPPL---PTEAPPPANPVSSPPPESSPPP 126
Query: 164 PAPTEITPNPPLVISKPHARYPTPADSWPSQLLGEQPTS 202
P PTE P P+ P P P +S PS + P++
Sbjct: 127 PPPTEAPPTTPITSPSPPTNPPPPPESPPSLPAPDPPSN 165
Score = 35.0 bits (79), Expect = 0.027
Identities = 29/95 (30%), Positives = 38/95 (39%), Gaps = 7/95 (7%)
Query: 112 PDLNAPPQLELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFR----PDLQAAYPAPT 167
P L +PP + L P Q +F G + PT +PT P P + P+ + P P
Sbjct: 16 PSLASPPLMALPPP-QPSFPGDNATSPTREPTNGNPPETTNTPAQSSPPPETPLSSPPPE 74
Query: 168 EITPNPPLVISKPHA--RYPTPADSWPSQLLGEQP 200
P+P L P P P S P L E P
Sbjct: 75 PSPPSPSLTGPPPTTIPVSPPPEPSPPPPLPTEAP 109
>At5g10570 putative bHLH transcription factor (bHLH061)
Length = 315
Score = 34.7 bits (78), Expect = 0.036
Identities = 31/102 (30%), Positives = 49/102 (47%), Gaps = 7/102 (6%)
Query: 16 EKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQIQSLKKENVSLLSES 75
E+ +R+ LN+ L L ++ + SIL +A +K+LL +I L+++ L S S
Sbjct: 156 ERRRRKRLNDR-LSLLRSIVPKITKMDRTSILGDAIDYMKELLDKINKLQEDEQELGSNS 214
Query: 76 HYVTMEKNELKEENSSLETQIEKLQGEIQARI---AQSKPDL 114
H T+ NE NS + E Q E+ I +KP L
Sbjct: 215 HLSTLITNESMVRNS---LKFEVDQREVNTHIDICCPTKPGL 253
>At3g22120 unknown protein
Length = 351
Score = 34.7 bits (78), Expect = 0.036
Identities = 24/91 (26%), Positives = 39/91 (42%), Gaps = 4/91 (4%)
Query: 112 PDLNAPPQLELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITP 171
P + PP+ ++ P+ + P V P + PTV P +P +P P + P
Sbjct: 51 PAPHKPPKHPVKPPKPPAVKPP--KPPAVKPPTPKPPTVKPHP-KPPTVKPHPKPPTVKP 107
Query: 172 NP-PLVISKPHARYPTPADSWPSQLLGEQPT 201
+P P + PH + PT P + + PT
Sbjct: 108 HPKPPTVKPPHPKPPTKPHPHPKPPIVKPPT 138
>At1g70460 putative protein kinase
Length = 710
Score = 34.3 bits (77), Expect = 0.046
Identities = 25/90 (27%), Positives = 35/90 (38%), Gaps = 7/90 (7%)
Query: 117 PPQLELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLV 176
PP P S LP++ P + P + P P L ++ P P ++TP PP
Sbjct: 31 PPTDSAPPPSPPADSSPPPALPSLPPAVFSPPPTVSSPPPPPLDSSPPPPPDLTP-PPSS 89
Query: 177 ISKPHAR------YPTPADSWPSQLLGEQP 200
P A +P P DS P + P
Sbjct: 90 PPPPDAPPPIPIVFPPPIDSPPPESTNSPP 119
>At1g06850 bZip transcription factor AtbZip52
Length = 337
Score = 34.3 bits (77), Expect = 0.046
Identities = 25/113 (22%), Positives = 57/113 (50%), Gaps = 1/113 (0%)
Query: 15 REKMKREHLNELF-LDLANALDLSEPNNGKASILIEASRLLKDLLCQIQSLKKENVSLLS 73
++ M E L+EL+ +D A + A +R +++L ++QSL+ E +L +
Sbjct: 133 KKAMPPEKLSELWNIDPKRAKRILANRQSAARSKERKARYIQELERKVQSLQTEATTLSA 192
Query: 74 ESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQLELESPE 126
+ + N L EN+ L+ +++ ++ + Q R A ++ ++++E+ E
Sbjct: 193 QLTLYQRDTNGLANENTELKLRLQAMEQQAQLRNALNEALRKEVERMKMETGE 245
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.309 0.128 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,644,001
Number of Sequences: 26719
Number of extensions: 197723
Number of successful extensions: 1310
Number of sequences better than 10.0: 191
Number of HSP's better than 10.0 without gapping: 65
Number of HSP's successfully gapped in prelim test: 126
Number of HSP's that attempted gapping in prelim test: 1033
Number of HSP's gapped (non-prelim): 343
length of query: 203
length of database: 11,318,596
effective HSP length: 94
effective length of query: 109
effective length of database: 8,807,010
effective search space: 959964090
effective search space used: 959964090
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146548.2