BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146527.10 - phase: 0 /pseudo
(129 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g05200 putative non-LTR retroelement reverse transcriptase 41 2e-04
At2g17610 putative non-LTR retroelement reverse transcriptase 40 2e-04
At1g43730 hypothetical protein 39 6e-04
At1g31100 hypothetical protein 39 7e-04
At1g23990 reverse transcriptase, putative 39 7e-04
At2g15540 putative non-LTR retroelement reverse transcriptase 39 0.001
At4g09710 RNA-directed DNA polymerase -like protein 38 0.002
At3g31420 hypothetical protein 38 0.002
At3g30570 putative reverse transcriptase 38 0.002
At1g29650 reverse transcriptase, putative 38 0.002
At2g17910 putative non-LTR retroelement reverse transcriptase 36 0.005
At1g47910 reverse transcriptase, putative 36 0.005
At1g47850 hypothetical protein 36 0.006
At1g24640 hypothetical protein 36 0.006
At3g44650 putative protein 35 0.010
At2g41580 putative non-LTR retroelement reverse transcriptase 35 0.010
At1g31030 F17F8.5 35 0.010
At2g19100 putative non-LTR retroelement reverse transcriptase 35 0.014
At1g25430 hypothetical protein 35 0.014
At2g40680 putative non-LTR retroelement reverse transcriptase 34 0.018
>At2g05200 putative non-LTR retroelement reverse transcriptase
Length = 1229
Score = 40.8 bits (94), Expect = 2e-04
Identities = 33/117 (28%), Positives = 50/117 (42%), Gaps = 6/117 (5%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGSS- 59
++ S L +L KY + + S S W SII + ILKEG W I +G
Sbjct: 768 LLNSPESLLSRILLGKYCHSSSFMECKLPSQPSHGWRSIIAGREILKEGLGWLITNGEKV 827
Query: 60 SFWFSNWNSLG-PIGSYVPIIDIH-DLHLSV---RDVLSAPGNHTQVLYTNLPSAVE 111
S W W S+ P+ P + H DL +S ++ L N V+ N + ++
Sbjct: 828 SIWNDPWLSISKPLVPIGPALREHQDLRVSALINQNTLQWDWNKIAVILPNYENLIK 884
>At2g17610 putative non-LTR retroelement reverse transcriptase
Length = 773
Score = 40.4 bits (93), Expect = 2e-04
Identities = 27/93 (29%), Positives = 39/93 (41%), Gaps = 1/93 (1%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGSS- 59
++Q L + KY L A A+S SS W SI+ ++ G + G+G++
Sbjct: 328 ILQQPFSLMARVFKAKYFPKERLLDAKATSQSSYAWKSILHGTKLISRGLKYIAGNGNNI 387
Query: 60 SFWFSNWNSLGPIGSYVPIIDIHDLHLSVRDVL 92
W NW L P V D L V D+L
Sbjct: 388 QLWKDNWLPLNPPRPPVGTCDSIYSQLKVSDLL 420
>At1g43730 hypothetical protein
Length = 320
Score = 39.3 bits (90), Expect = 6e-04
Identities = 32/126 (25%), Positives = 52/126 (40%), Gaps = 17/126 (13%)
Query: 6 NKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSG-SSSFWFS 64
N L S L + G N +++++ S W + + + + + +GSG ++ FW
Sbjct: 23 NVLLASELVENFLGQRNFWTLNSTTSDSWIWRRLCKLREVARPFVVCDVGSGVTAKFWHD 82
Query: 65 NWNS-------LGPIG--------SYVPIIDI-HDLHLSVRDVLSAPGNHTQVLYTNLPS 108
NW +GP+G V +ID HD + L AP N T+L
Sbjct: 83 NWTGHGPLIDLVGPLGPQTVGLPIDAVGLIDCQHDDSFIWKTDLHAPSNIFSTAKTSLAL 142
Query: 109 AVENHI 114
+NHI
Sbjct: 143 HPQNHI 148
>At1g31100 hypothetical protein
Length = 1090
Score = 38.9 bits (89), Expect = 7e-04
Identities = 30/104 (28%), Positives = 44/104 (41%), Gaps = 5/104 (4%)
Query: 29 SSNSSPTWSSIIRAKNILKEGYTWRIGSGSS-SFWFSNWNSLGPI----GSYVPIIDIHD 83
S+ S TW S++R + + +G G SFWF NW+ LGP+ GS P
Sbjct: 793 SATDSWTWRSLLRLRTVAGRFLFSVLGDGKKISFWFDNWSPLGPLFKLFGSSGPRALCIP 852
Query: 84 LHLSVRDVLSAPGNHTQVLYTNLPSAVENHINNIHIQFNDNIED 127
+ V D S G T+ A+ H+ I + D+ D
Sbjct: 853 IQAKVADACSDVGWLISPPRTDQALALLIHLTTIALPCFDSSPD 896
>At1g23990 reverse transcriptase, putative
Length = 657
Score = 38.9 bits (89), Expect = 7e-04
Identities = 21/79 (26%), Positives = 42/79 (52%), Gaps = 4/79 (5%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTL--LASASSNSSPTWSSIIRAKNILKEGYTWRIGSGS 58
+V TN LWV + +Y H+T + + ++ S W +++ ++I K+ + +G
Sbjct: 343 IVSHTNSLWVRWIE-QYLLKHDTFWSVQTTTNMGSWMWRKLLKYRDIAKQFCKVEVKNGK 401
Query: 59 S-SFWFSNWNSLGPIGSYV 76
+FW+ NW++LG + V
Sbjct: 402 RVAFWYENWSNLGVLNDLV 420
>At2g15540 putative non-LTR retroelement reverse transcriptase
Length = 1225
Score = 38.5 bits (88), Expect = 0.001
Identities = 26/93 (27%), Positives = 42/93 (44%), Gaps = 1/93 (1%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGS-S 59
+++ N L +L +Y + + ++ S W SI+ AK +L G IGSG +
Sbjct: 753 LIRFPNSLLSRVLRGRYFRYSDPIQIGKANRPSFGWRSIMAAKPLLLSGLRRTIGSGMLT 812
Query: 60 SFWFSNWNSLGPIGSYVPIIDIHDLHLSVRDVL 92
W W P I++I D HL V D++
Sbjct: 813 RVWEDPWIPSFPPRPAKSILNIRDTHLYVNDLI 845
>At4g09710 RNA-directed DNA polymerase -like protein
Length = 1274
Score = 37.7 bits (86), Expect = 0.002
Identities = 27/95 (28%), Positives = 44/95 (45%), Gaps = 13/95 (13%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTLLASAS-SNSSPTWSSIIRAKNILKEGYTWRIGSGSS 59
+++ + L +L KY + + SAS S +S W I+ +++L++G W IG G S
Sbjct: 807 ILKEPHSLLSRVLLGKYCNTSSFMDCSASPSFASHGWRGILAGRDLLRKGLGWSIGQGDS 866
Query: 60 -SFWFSNWNSLGPIGSYVPI---------IDIHDL 84
+ W W L P PI + +HDL
Sbjct: 867 INVWTEAW--LSPSSPQTPIGPPTETNKDLSVHDL 899
>At3g31420 hypothetical protein
Length = 1491
Score = 37.7 bits (86), Expect = 0.002
Identities = 20/67 (29%), Positives = 32/67 (46%), Gaps = 1/67 (1%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGS-S 59
++Q N L +L +Y N + A +S W SI+ +N+LK+G +G GS
Sbjct: 1031 LLQHPNCLMARVLRGRYFPETNVMNAVQGRRASFVWKSILHGRNLLKKGLRCCVGDGSLI 1090
Query: 60 SFWFSNW 66
+ W W
Sbjct: 1091 NAWLDPW 1097
>At3g30570 putative reverse transcriptase
Length = 1099
Score = 37.7 bits (86), Expect = 0.002
Identities = 24/76 (31%), Positives = 36/76 (46%), Gaps = 4/76 (5%)
Query: 1 MVQSTNKLWVSLLSNKYTGG--HNTLLASASSNSSPTWSSI-IRAKNILKEGYTWRIGSG 57
++ + L V ++ KY G + A SN S TW S+ I + ++ GY+W IG G
Sbjct: 811 VLHDISSLLVQVVRKKYKVGLIQDQSWMVAKSNWSSTWRSVGIGIREVVYPGYSWVIGDG 870
Query: 58 SS-SFWFSNWNSLGPI 72
FW W S P+
Sbjct: 871 RQIRFWRDKWLSSTPL 886
>At1g29650 reverse transcriptase, putative
Length = 1557
Score = 37.7 bits (86), Expect = 0.002
Identities = 27/97 (27%), Positives = 47/97 (47%), Gaps = 12/97 (12%)
Query: 9 WVSLLSNKYTGGH-----NTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGSS-SFW 62
W + +K GG + L A + S W S++ +++L +G IG+G+S S W
Sbjct: 1150 WEKMCLSKENGGRYFPDEDFLEADLGARPSYAWRSVLHGRDLLTKGLRKEIGNGNSISVW 1209
Query: 63 FSNWNSLGPIGSYVPIIDIH---DLHLSVRDVLSAPG 96
NW + G +P + H L+L V+D+++ G
Sbjct: 1210 MDNW--IYDNGPRIP-LQKHFSVSLNLRVKDLINLDG 1243
>At2g17910 putative non-LTR retroelement reverse transcriptase
Length = 1344
Score = 36.2 bits (82), Expect = 0.005
Identities = 18/67 (26%), Positives = 32/67 (46%), Gaps = 1/67 (1%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGSSS 60
++Q L+ + ++Y + L A+ S S W SI+ + +L +G IG+G +
Sbjct: 851 VLQEKGSLFSRVFQSRYFSNSDFLSATRGSRPSYAWRSILFGRELLMQGLRTVIGNGQKT 910
Query: 61 F-WFSNW 66
F W W
Sbjct: 911 FVWTDKW 917
>At1g47910 reverse transcriptase, putative
Length = 1142
Score = 36.2 bits (82), Expect = 0.005
Identities = 19/67 (28%), Positives = 36/67 (53%), Gaps = 1/67 (1%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGSS- 59
++ + + L+ + +Y N L + S + S W S+I A++++ +G R+GSG+S
Sbjct: 657 LITAPDSLFAKVFKGRYFRKSNPLDSIKSYSPSYGWRSMISARSLVYKGLIKRVGSGASI 716
Query: 60 SFWFSNW 66
S W W
Sbjct: 717 SVWNDPW 723
>At1g47850 hypothetical protein
Length = 799
Score = 35.8 bits (81), Expect = 0.006
Identities = 17/45 (37%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Query: 29 SSNSSPTWSSIIRAKNILKEGYTWRIGSGSS-SFWFSNWNSLGPI 72
SS S W +++ +++ K I SGSS SFW+ NW+ LG +
Sbjct: 496 SSLGSWMWKKLLKYRDVAKSMCKVEIKSGSSTSFWYDNWSQLGQL 540
>At1g24640 hypothetical protein
Length = 1270
Score = 35.8 bits (81), Expect = 0.006
Identities = 19/56 (33%), Positives = 29/56 (50%), Gaps = 1/56 (1%)
Query: 12 LLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGSSSF-WFSNW 66
LL ++Y + L A+ S S W SI+ + +L +G R+G G+S F W W
Sbjct: 847 LLKSRYFDATDFLDAALSQRPSFGWRSILFGRELLSKGLQKRVGDGASLFVWIDPW 902
>At3g44650 putative protein
Length = 762
Score = 35.0 bits (79), Expect = 0.010
Identities = 21/89 (23%), Positives = 41/89 (45%), Gaps = 2/89 (2%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTLLASASSN-SSPTWSSIIRAKNILKEGYTWRIGSG-S 58
++ + LWV + + + ++N S W I++ + + K +G+G S
Sbjct: 447 IISHGDSLWVKWVEHNLLKREIFWIVKENANLGSWIWKKILKYRGVAKRFCKAEVGNGES 506
Query: 59 SSFWFSNWNSLGPIGSYVPIIDIHDLHLS 87
+SFWF +W+ LG + I D+ +S
Sbjct: 507 TSFWFDDWSLLGRLIDVAGIRGTIDMGIS 535
>At2g41580 putative non-LTR retroelement reverse transcriptase
Length = 1094
Score = 35.0 bits (79), Expect = 0.010
Identities = 17/63 (26%), Positives = 32/63 (49%), Gaps = 1/63 (1%)
Query: 5 TNKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGSSSF-WF 63
++ L +L ++Y + L A+ + S W SI+ + +L G IG+G +++ W
Sbjct: 606 SDSLLSQILKSRYYMNSDFLSATKGTRPSYAWQSILYGRELLVSGLKKIIGNGENTYVWM 665
Query: 64 SNW 66
NW
Sbjct: 666 DNW 668
>At1g31030 F17F8.5
Length = 872
Score = 35.0 bits (79), Expect = 0.010
Identities = 18/73 (24%), Positives = 41/73 (55%), Gaps = 4/73 (5%)
Query: 1 MVQSTNKLWVSLLSNKYTGGHNTL--LASASSNSSPTWSSIIRAKNILKEGYTWRIGSG- 57
++ ++N LW ++ +Y ++ L ++S S W I++ +++ K +G+G
Sbjct: 541 IISNSNSLWTKWVA-EYLIRKKSIWSLKQSTSMGSWIWRKILKIRDVAKSFSRVEVGNGE 599
Query: 58 SSSFWFSNWNSLG 70
S+SFW+ +W++ G
Sbjct: 600 SASFWYDHWSAHG 612
>At2g19100 putative non-LTR retroelement reverse transcriptase
Length = 1447
Score = 34.7 bits (78), Expect = 0.014
Identities = 22/91 (24%), Positives = 46/91 (50%), Gaps = 3/91 (3%)
Query: 6 NKLWVSLL-SNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGS-SSFWF 63
+ LWV + +N ++ S+++ S W +++ + + K + +G +SFW+
Sbjct: 1123 DSLWVKWIETNLLKNNSFWIIKSSTTMGSWMWKKLLKYREVAKTFSKIEVHNGQRTSFWY 1182
Query: 64 SNWNSLGPIGSYVPIIDIHDLHLSVRDVLSA 94
+W+ +G I +++ I DL + RD L A
Sbjct: 1183 DDWSCMGRIMNFIGDKGIIDLGIK-RDKLVA 1212
>At1g25430 hypothetical protein
Length = 1213
Score = 34.7 bits (78), Expect = 0.014
Identities = 12/43 (27%), Positives = 25/43 (57%), Gaps = 1/43 (2%)
Query: 31 NSSPTWSSIIRAKNILKEGYTWRIGSG-SSSFWFSNWNSLGPI 72
+ S TW ++ + + + ++G+G + +W+ NW SLGP+
Sbjct: 917 SDSWTWKRLLSLRPLAHQFLVCKVGNGLKADYWYDNWTSLGPL 959
>At2g40680 putative non-LTR retroelement reverse transcriptase
Length = 296
Score = 34.3 bits (77), Expect = 0.018
Identities = 13/36 (36%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Query: 36 WSSIIRAKNILKEGYTWRIGSG-SSSFWFSNWNSLG 70
W +++ +++ K+ Y I SG +SFW+ W+SLG
Sbjct: 2 WRKLLKLRDLAKDFYQVEIKSGRGTSFWYDTWSSLG 37
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.314 0.130 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,179,213
Number of Sequences: 26719
Number of extensions: 130207
Number of successful extensions: 316
Number of sequences better than 10.0: 73
Number of HSP's better than 10.0 without gapping: 44
Number of HSP's successfully gapped in prelim test: 29
Number of HSP's that attempted gapping in prelim test: 269
Number of HSP's gapped (non-prelim): 76
length of query: 129
length of database: 11,318,596
effective HSP length: 88
effective length of query: 41
effective length of database: 8,967,324
effective search space: 367660284
effective search space used: 367660284
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146527.10