BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146333.5 + phase: 0
(501 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g03780 putative athila-like protein 32 0.63
At1g32810 hypothetical protein 32 0.63
At5g41020 putative protein 32 0.82
At5g53020 putative protein 32 1.1
At3g02120 hypothetical protein 32 1.1
At2g18540 putative vicilin storage protein (globulin-like) 32 1.1
At5g60030 KED - like protein 31 1.4
At5g49030 isoleucyl-tRNA synthetase 31 1.4
At1g65280 31 1.4
At1g49870 hypothetical protein 31 1.4
At1g79830 unknown protein (At1g79830) 31 1.8
At4g31340 unknown protein 30 2.4
At1g73860 kinesin-related protein 30 2.4
At1g15560 hypothetical protein 30 2.4
At5g03060 putative protein 30 3.1
At3g20250 RNA-binding protein, putative 30 3.1
At2g24500 putative C2H2-type zinc finger protein 30 3.1
At1g16690 Unknown protein 30 3.1
At5g41140 putative protein 30 4.1
At5g19900 PRLI-interacting factor A 30 4.1
>At4g03780 putative athila-like protein
Length = 489
Score = 32.3 bits (72), Expect = 0.63
Identities = 43/168 (25%), Positives = 65/168 (38%), Gaps = 42/168 (25%)
Query: 333 PTGQREAFTRAWSKVRWKSVKHLGVRSGIAHEAYTQWVINRAEEIGLPYPAMRHVAASAP 392
P + E FTR W + HL RS + E+ ++ EEI +P
Sbjct: 311 PIKEDEDFTREWFAPEDQKGVHLEERS-LESESLSE------EEISIP----------TY 353
Query: 393 SIPLPLPPTTQEMYQEHLAMES-REK--QMWKARYNEAENLIMTLDGKDEQKTHENLMLK 449
S P PPT +E L ++S EK ++W YN E+ I GK K
Sbjct: 354 SPGEPKPPTNPSAEEEQLILDSFAEKLLELWIG-YNAQEDTI----GKSTHK-------- 400
Query: 450 KELVKVRRELEEKDELLMQDSKRARGRRNFYARYCGSDSESESEDHPT 497
+EE + +++ + +G NF + SE ED+ T
Sbjct: 401 ---------MEELNSTILELQEMVKGYHNFEIEDWSKEPGSEEEDYST 439
>At1g32810 hypothetical protein
Length = 654
Score = 32.3 bits (72), Expect = 0.63
Identities = 15/63 (23%), Positives = 32/63 (49%)
Query: 436 GKDEQKTHENLMLKKELVKVRRELEEKDELLMQDSKRARGRRNFYARYCGSDSESESEDH 495
GK + + L ++++ +KV R+ ++E+ +D + A + + +C + E E E H
Sbjct: 575 GKRKARKRRRLSIQRKGIKVLRKKRNEEEVSEEDEEDAFSDTSEESIFCDEEEEEEEEGH 634
Query: 496 PTT 498
T
Sbjct: 635 TAT 637
>At5g41020 putative protein
Length = 588
Score = 32.0 bits (71), Expect = 0.82
Identities = 20/96 (20%), Positives = 47/96 (48%), Gaps = 7/96 (7%)
Query: 401 TTQEMYQEHLAMESREKQMWKARYNEAENLI---MTLDGKDEQKTHENLMLKKELVKVRR 457
T +E EH +M+ +EK+ K + + M + G++ +K + + + K+ K ++
Sbjct: 21 TGEEFISEHSSMKDKEKKRKKNKRENKDGFTGEDMEITGRESEKLGDEVFIVKKKKKSKK 80
Query: 458 ELEEKDELLMQDSKRARGRRNFYARYCGSDSESESE 493
+ E + K+++ R ++ +DSE+E +
Sbjct: 81 PIRIDSEAVDAVKKKSKKR----SKETKADSEAEDD 112
>At5g53020 putative protein
Length = 720
Score = 31.6 bits (70), Expect = 1.1
Identities = 23/80 (28%), Positives = 40/80 (49%), Gaps = 3/80 (3%)
Query: 398 LPPTTQEMYQEHLAMESREKQMWKARYNEAENLIMTLDGKDEQ---KTHENLMLKKELVK 454
L T + QEH + R+K+ + + L L ++Q K HE L E+ K
Sbjct: 146 LRETQERDVQEHSSELWRQKKTFLELASSQRQLEAELSRANKQIEAKGHELEDLSLEINK 205
Query: 455 VRRELEEKDELLMQDSKRAR 474
+R++LE+KD +L K+++
Sbjct: 206 MRKDLEQKDRILAVMMKKSK 225
>At3g02120 hypothetical protein
Length = 480
Score = 31.6 bits (70), Expect = 1.1
Identities = 21/89 (23%), Positives = 37/89 (40%), Gaps = 4/89 (4%)
Query: 386 HVAASAPSIPLPLPPTTQEMYQEHLAMESREKQMWKARY----NEAENLIMTLDGKDEQK 441
HV S+PLP P Y++ E Q+ + NE E + ++ DE +
Sbjct: 269 HVITEINSVPLPPPVKKPNEYEKETIAEKEPYQVENSNSDIEDNEEEEKMSEVESDDEHE 328
Query: 442 THENLMLKKELVKVRRELEEKDELLMQDS 470
+ M++ E + + +E E +DS
Sbjct: 329 EEQTDMIEAEALVEKEVIETVKENKPEDS 357
>At2g18540 putative vicilin storage protein (globulin-like)
Length = 699
Score = 31.6 bits (70), Expect = 1.1
Identities = 19/72 (26%), Positives = 36/72 (49%), Gaps = 6/72 (8%)
Query: 403 QEMYQEHLAMESREKQMWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRRELEEK 462
Q +E + + RE+Q K R EA ++E+K E + ++E + R+E EE
Sbjct: 530 QRKEREEVERKRREEQERKRREEEARKR------EEERKREEEMAKRREQERQRKEREEV 583
Query: 463 DELLMQDSKRAR 474
+ + ++ +R R
Sbjct: 584 ERKIREEQERKR 595
>At5g60030 KED - like protein
Length = 292
Score = 31.2 bits (69), Expect = 1.4
Identities = 23/93 (24%), Positives = 50/93 (53%), Gaps = 3/93 (3%)
Query: 403 QEMYQEHLAMESREKQMWKARYNEAENLIMTLDG-KDEQKTHENLMLKKELVKVRRELEE 461
+++ E + + +E++ K++ N E+++ + +DEQK+ E KK K ++E
Sbjct: 171 EKLEDEQKSADRKERKKKKSKKNNDEDVVDEKEKLEDEQKSAEIKEKKKN--KDEDVVDE 228
Query: 462 KDELLMQDSKRARGRRNFYARYCGSDSESESED 494
K++ ++D +R+ R+ + SD E SE+
Sbjct: 229 KEKEKLEDEQRSGERKKEKKKKRKSDEEIVSEE 261
>At5g49030 isoleucyl-tRNA synthetase
Length = 1093
Score = 31.2 bits (69), Expect = 1.4
Identities = 32/134 (23%), Positives = 53/134 (38%), Gaps = 15/134 (11%)
Query: 270 ITPAAQVKEIITGCGDFLNVPLLGTRGG-----INYNPELAMRQ-----FGFPMKTKPIN 319
++P + G F + +LG ++ N L M + + + +TK
Sbjct: 475 VSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPT 534
Query: 320 LATSPEFFYYSNAPTGQREAFTRAWSKVRWKSVKHLGV-RSGIAHEAYTQWVINRAEEIG 378
+ + E ++ S G R A A + V+W V H V R + + W I+R G
Sbjct: 535 IFRATEQWFAS--VEGFRTATMDAINNVKW--VPHQAVNRISAMTSSRSDWCISRQRTWG 590
Query: 379 LPYPAMRHVAASAP 392
+P PA HV P
Sbjct: 591 VPIPAFYHVKTKEP 604
>At1g65280
Length = 605
Score = 31.2 bits (69), Expect = 1.4
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 4/54 (7%)
Query: 449 KKELVKVRRELEEKDELLMQDSKRARGRRNFYAR----YCGSDSESESEDHPTT 498
K+E + R+++E K+ K+ + ++ Y Y GSDS S+ ED P T
Sbjct: 60 KREKERKRKKIERKERKRRDMKKKKKTKKREYESDTESYSGSDSFSDQEDDPET 113
>At1g49870 hypothetical protein
Length = 828
Score = 31.2 bits (69), Expect = 1.4
Identities = 18/56 (32%), Positives = 32/56 (57%), Gaps = 2/56 (3%)
Query: 408 EHLAMESREKQMWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRRELEEKD 463
+H+ +EKQM+KA E EN +G Q HE++ + K ++ RR++ +K+
Sbjct: 639 KHVPKLEKEKQMFKAGVEETENSKRNNEGSLNQ-GHESMKVAKPILS-RRQMRDKE 692
>At1g79830 unknown protein (At1g79830)
Length = 918
Score = 30.8 bits (68), Expect = 1.8
Identities = 31/112 (27%), Positives = 50/112 (43%), Gaps = 8/112 (7%)
Query: 390 SAPSIPLPLPPTTQEMYQEHLAMESREKQMWKARYNEAENLIM-----TLDGKDEQKTHE 444
S PS P+ L E Q +S EK+ +A N E L TL+G+ Q E
Sbjct: 670 SLPSSPIQLSCLRAEQGQ---LSKSLEKERQRAAENRQEYLAAKEEADTLEGRANQLEVE 726
Query: 445 NLMLKKELVKVRRELEEKDELLMQDSKRARGRRNFYARYCGSDSESESEDHP 496
L+++ + +E+ +EL+ +D +R + R R +S + SE P
Sbjct: 727 IRELRRKHKQELQEVLLHNELIQKDLEREKASRLDLERTARINSSAVSEQLP 778
>At4g31340 unknown protein
Length = 437
Score = 30.4 bits (67), Expect = 2.4
Identities = 17/70 (24%), Positives = 36/70 (51%)
Query: 404 EMYQEHLAMESREKQMWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRRELEEKD 463
E+ + L +++EK +AR NEAE + L+ ++ N K ++ K+ R ++ +
Sbjct: 122 EVLKNFLEQKNKEKDSTEARTNEAEKKLRELNSSLDKLQKTNEEQKNKIGKLERAIKIAE 181
Query: 464 ELLMQDSKRA 473
E +++ A
Sbjct: 182 EEMLRTKLEA 191
>At1g73860 kinesin-related protein
Length = 1050
Score = 30.4 bits (67), Expect = 2.4
Identities = 15/59 (25%), Positives = 32/59 (53%)
Query: 416 EKQMWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRRELEEKDELLMQDSKRAR 474
EK MW+ + E ++ L +++Q E LK+EL +R+ E++ + +K+++
Sbjct: 238 EKSMWEEKKKHEEEDMVKLMKQNDQHNLEISALKQELETTKRKYEQQYSQIESQTKKSK 296
>At1g15560 hypothetical protein
Length = 399
Score = 30.4 bits (67), Expect = 2.4
Identities = 16/56 (28%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Query: 88 TFPDFQLVPTLEAYSHLLDSPIAEKTPFTGPGASLTPLVIAK--DLYLKTSDVSKH 141
T+ D + E Y LL +A+ F G +++ PL I + +Y++ + +SKH
Sbjct: 229 TYVDEKARVIAEKYDELLQERMAQMQLFDGENSTMNPLTIEEKNKIYVQVAGISKH 284
>At5g03060 putative protein
Length = 292
Score = 30.0 bits (66), Expect = 3.1
Identities = 22/94 (23%), Positives = 45/94 (47%), Gaps = 2/94 (2%)
Query: 400 PTTQEMYQEHLAMESREKQMWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRREL 459
PT + + + + ++ ++ K Y E +++ +Q N ++K + K +EL
Sbjct: 23 PTRRNLVVQEEILATKH-EILKENYENLEKDYKSIEESFKQMNEMNEIMKFQYQKQIKEL 81
Query: 460 EEKDELLMQDSKRARGRRNFYARYC-GSDSESES 492
EEK L++D ++ R + Y + G SE E+
Sbjct: 82 EEKILSLLKDLEKERSEKEEYMKEMKGMISEKEA 115
>At3g20250 RNA-binding protein, putative
Length = 955
Score = 30.0 bits (66), Expect = 3.1
Identities = 31/117 (26%), Positives = 48/117 (40%), Gaps = 14/117 (11%)
Query: 361 IAHEAYTQWVINRAEEIGLPYPAMRHVAASAPSIPLPLPPTTQEMYQEHLAMESREKQMW 420
+AH+ Y +V E G P + + L +M Q A EK +
Sbjct: 818 LAHDQYGNYVTQHVLERGKPDERRQIIEK--------LTGNVVQMSQHKYASNVVEKCLE 869
Query: 421 KARYNEAENLIMTLDGKDEQKTHENLMLKKELVK--VRRELE----EKDELLMQDSK 471
A E E LI + GK E+ H M+K + V++ LE ++ E+L+Q K
Sbjct: 870 HADSTEREFLIEEIMGKSEEDNHLLAMMKDQFANYVVQKVLEISKDQQREILVQRMK 926
>At2g24500 putative C2H2-type zinc finger protein
Length = 395
Score = 30.0 bits (66), Expect = 3.1
Identities = 27/101 (26%), Positives = 46/101 (44%), Gaps = 8/101 (7%)
Query: 398 LPPTTQEMYQEHLAMESREKQMWKARYNEAENLI---MTLDGKDEQKTHE-NLMLKKELV 453
+P T+ +++ A ++EK + EA L + G K HE +L K ++
Sbjct: 39 VPGVTEALFEARQAAIAQEK----VKAVEAPMLYSCGICNKGYRSSKAHEQHLKSKSHVL 94
Query: 454 KVRRELEEKDELLMQDSKRARGRRNFYARYCGSDSESESED 494
K E+D+ +++ R +N A+ GS E ESED
Sbjct: 95 KASTSTGEEDKAIIKQLPPRRVEKNNTAQLKGSIEEEESED 135
>At1g16690 Unknown protein
Length = 439
Score = 30.0 bits (66), Expect = 3.1
Identities = 36/162 (22%), Positives = 75/162 (46%), Gaps = 16/162 (9%)
Query: 350 KSVKHLGVRSGIAHEAYTQWVINRAEEIGLPYPAMRHVAASAP---SIPLPLPPTTQEMY 406
+++K+L ++ G+ H Y+ W R EI P +R + P + P + ++ +
Sbjct: 173 EALKYLSIKYGVFHAIYSYWKNKR--EI-WQKPILRRLQPPPPVNDTNPYNVFRPREKAH 229
Query: 407 QEHL-AMESRE------KQMWKARYN--EAENLIMTLDGKDEQKTHENLMLKKELVKVRR 457
+ H M+ RE +++ + R N +A+ ++ L K E+K + + + L +++
Sbjct: 230 RLHARRMQQRENNAQSFEKLRQVRRNLDQAKTILEALI-KREEKKRDFMASEVSLQRIQL 288
Query: 458 ELEEKDELLMQDSKRARGRRNFYARYCGSDSESESEDHPTTS 499
+ + + ELL A + R+ S+ E D PTT+
Sbjct: 289 KYKNETELLEDSLALAGFPLSTAYRFGSSEDEFMDSDDPTTT 330
>At5g41140 putative protein
Length = 983
Score = 29.6 bits (65), Expect = 4.1
Identities = 25/108 (23%), Positives = 47/108 (43%), Gaps = 9/108 (8%)
Query: 357 VRSGIAHEAYTQWVINRAEEIGLPYPAMRHVAASAPSIPLPLPPTTQEMYQEHLAMESRE 416
V + + HE + R +EI + + S+ L Q + E A+ +
Sbjct: 725 VNADLTHE-----ITRRKDEIEILRLDLEETRKSSMETEASLSEELQRIIDEKEAVITAL 779
Query: 417 KQMWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRRELEEKDE 464
K + +NL +L + + + L+K++V+VR ELE+K+E
Sbjct: 780 KSQLETAIAPCDNLKHSLSNNESEIEN----LRKQVVQVRSELEKKEE 823
>At5g19900 PRLI-interacting factor A
Length = 494
Score = 29.6 bits (65), Expect = 4.1
Identities = 20/70 (28%), Positives = 31/70 (43%)
Query: 425 NEAENLIMTLDGKDEQKTHENLMLKKELVKVRRELEEKDELLMQDSKRARGRRNFYARYC 484
N EN + D Q ENL LK+ L + REL + L +R ++
Sbjct: 306 NVLENRVDDQDSHIAQLEEENLTLKERLFLMERELGDMRRRLQYLERRDMVAQDANEEVV 365
Query: 485 GSDSESESED 494
++SES+ +D
Sbjct: 366 ENESESDGDD 375
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,766,714
Number of Sequences: 26719
Number of extensions: 531848
Number of successful extensions: 1577
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 1560
Number of HSP's gapped (non-prelim): 40
length of query: 501
length of database: 11,318,596
effective HSP length: 103
effective length of query: 398
effective length of database: 8,566,539
effective search space: 3409482522
effective search space used: 3409482522
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146333.5