BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146332.8 - phase: 0 /pseudo
(199 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11590 unknown protein 96 1e-20
At5g22310 unknown protein 68 4e-12
At5g50840 unknown protein 30 0.84
At1g10890 unknown protein 30 0.84
At2g23820 unknown protein 30 1.1
At2g47330 putative ATP-dependent RNA helicase 29 1.4
At1g69160 unknown protein 29 1.9
At5g21940 unknown protein 28 2.5
At5g06810 putative protein 28 2.5
At1g20930 putative cell division control protein, cdc2 kinase 28 2.5
At5g65470 unknown protein 28 3.2
At4g18390 teosinte branched1 like protein 28 3.2
At4g09360 putative protein 28 3.2
At4g01350 putative CHP-rich zinc finger protein 28 3.2
At3g19440 unknown protein 28 3.2
At2g25000 putative WRKY-type DNA binding protein 28 3.2
At5g35450 disease resistance protein 28 4.2
At2g42470 hypothetical protein 28 4.2
At2g16900 unknown protein 28 4.2
At1g76540 putative cell division control protein cdc2 28 4.2
>At3g11590 unknown protein
Length = 622
Score = 95.9 bits (237), Expect = 1e-20
Identities = 72/174 (41%), Positives = 90/174 (51%), Gaps = 15/174 (8%)
Query: 15 KIRKRGCSS-TSSSSPFSRR-YRFKRAILMGKKSGYNTPAPMWKTSSKSPS-MATHHHHA 71
KIRKRGCSS TSS+S R YRFKRAI++GK+ G TP P W+ +SPS A+ HA
Sbjct: 16 KIRKRGCSSPTSSTSSILREGYRFKRAIVVGKRGGSTTPVPTWRLMGRSPSPRASGALHA 75
Query: 72 MKKTALH-SSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIKKES----MKSNKER- 125
+ H S+TG K VSARKLAATLWE+N++ R+ +E+ KS KER
Sbjct: 76 AASPSSHCGSKTG---KVSAPAPVSARKLAATLWEMNEMPSPRVVEEAAPMIRKSRKERI 132
Query: 126 ---DKVERLCRSVLLGPQKLDPLVSPFSERTKGIEVDGCKRNVSDLSHQFHFVD 176
S L P DP SP SER + +R S + D
Sbjct: 133 APLPPPRSSVHSGSLPPHLSDPSHSPVSERMERSGTGSRQRRASSTVQKLRLGD 186
>At5g22310 unknown protein
Length = 481
Score = 67.8 bits (164), Expect = 4e-12
Identities = 58/160 (36%), Positives = 79/160 (49%), Gaps = 36/160 (22%)
Query: 10 KQKHNKIRKRGCSSTSSSSPFSRRYRFKRAILMGKKS-----GYNTPAPMWKTSSKSPSM 64
++K KIRKRG SS+SSSS +RR RFKRAI GK++ G TP T++K+P +
Sbjct: 4 RKKGCKIRKRGGSSSSSSS-LARRNRFKRAIFAGKRAAQDDGGSGTPVKSI-TAAKTPVL 61
Query: 65 ATHH-------HHAMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIKKE 117
+ HH ++K+ VSARKLAATLWEIND + +
Sbjct: 62 LSFSPENLPIDHHQLQKSC-----------------VSARKLAATLWEINDDADPPVNSD 104
Query: 118 -----SMKSNKERDKVERLCRSVLLGPQKLDPLVSPFSER 152
S K ++ R K S+ P+ DP+ SER
Sbjct: 105 KDCLRSKKPSRYRAKKSTEFSSIDFPPRSSDPISRLSSER 144
>At5g50840 unknown protein
Length = 404
Score = 30.0 bits (66), Expect = 0.84
Identities = 18/49 (36%), Positives = 27/49 (54%), Gaps = 1/49 (2%)
Query: 87 KEKKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSV 135
KE + K TL E+ + R+KK K+ K++DK+E LCRS+
Sbjct: 339 KENAFLKNKTEKSDITLIELVE-ERERLKKLLEKTKKQKDKLESLCRSL 386
>At1g10890 unknown protein
Length = 592
Score = 30.0 bits (66), Expect = 0.84
Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 2/60 (3%)
Query: 88 EKKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDPLVS 147
++KE RK E+ +L R K+E+M+ K ++ ERL + LLG K P +S
Sbjct: 170 QRKEAMERQRKEEERYRELEEL--QRQKEEAMRRKKAEEEEERLKQMKLLGKNKSRPKLS 227
>At2g23820 unknown protein
Length = 243
Score = 29.6 bits (65), Expect = 1.1
Identities = 33/117 (28%), Positives = 48/117 (40%), Gaps = 9/117 (7%)
Query: 23 STSSSSPFSRRYRFKRAILMGKKSGYNTPAPMW-KTSSKSPSMATHHHHAMKKTALHSSR 81
S+SSSS S F K TP W K K P H + M AL SS
Sbjct: 69 SSSSSSSASSAIDFLSLCTRLK----TTPRAGWIKRDVKDPESIADHMYRMGLMALISSD 124
Query: 82 TGLPFKEKKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVLLG 138
++K +A + + D+TPS + K+ +E + +E +C+ LLG
Sbjct: 125 IPGVNRDKCMKMAIVHDIAEAI--VGDITPSCGISKEEKNRRESEALEHMCK--LLG 177
>At2g47330 putative ATP-dependent RNA helicase
Length = 760
Score = 29.3 bits (64), Expect = 1.4
Identities = 22/77 (28%), Positives = 37/77 (47%), Gaps = 1/77 (1%)
Query: 18 KRGCSSTSSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSKSPSMA-THHHHAMKKTA 76
K + + S+P ++ Y KR LMG SG M +T S++P +A T + + +
Sbjct: 680 KNSFVAATPSNPQNQAYPNKRPSLMGFVSGGTIGGDMGRTQSQAPPVAPTQNASSHNSSQ 739
Query: 77 LHSSRTGLPFKEKKEIS 93
HS + +E+K S
Sbjct: 740 NHSQSSENRPRERKRRS 756
>At1g69160 unknown protein
Length = 321
Score = 28.9 bits (63), Expect = 1.9
Identities = 24/112 (21%), Positives = 48/112 (42%), Gaps = 8/112 (7%)
Query: 19 RGCSSTSSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSKSPSMATHHHHAMKKTALH 78
R S+T++++ S ++++ SG+ TP P T +K+ ++ A K+
Sbjct: 180 RSTSTTTTTTASSS----SKSLISSSSSGFRTPPPYLNTPTKNYKQFLNYTSATKQVGEE 235
Query: 79 SSRTGLPFK---EKKEISVSARKLAATLWEINDLTPS-RIKKESMKSNKERD 126
++T + EK ++ S + + D+ RIK+E E D
Sbjct: 236 ETKTNKEYSWLDEKLKVMESLSENQRIWSDDEDIDDDRRIKREGEDDGMESD 287
>At5g21940 unknown protein
Length = 264
Score = 28.5 bits (62), Expect = 2.5
Identities = 22/75 (29%), Positives = 31/75 (41%), Gaps = 8/75 (10%)
Query: 8 HLKQKHNKIRKRGCSS-----TSSSSPFSRRYRFKRAILMGKKS---GYNTPAPMWKTSS 59
H ++NK RG S +SS S + ++ G+ S G ++P TS
Sbjct: 139 HQIWENNKTTPRGGISKKHVMSSSRSALTLAMAVAAGVMTGEGSSSGGDSSPGSSPTTSG 198
Query: 60 KSPSMATHHHHAMKK 74
P HH H MKK
Sbjct: 199 SPPRQLHHHQHQMKK 213
>At5g06810 putative protein
Length = 1141
Score = 28.5 bits (62), Expect = 2.5
Identities = 29/123 (23%), Positives = 48/123 (38%), Gaps = 10/123 (8%)
Query: 46 SGYNTPAPMWKTSSKSPSMATHHHHAMKKTALHSSRTGLPFKEKKEI-SVSARKLAATLW 104
S YN P K +HH + + + R G FKE +E+ S LA+ +
Sbjct: 151 SEYNHLVPCDKVFLDEEGFLLENHHVLCYSGVDPKRIGKIFKEAREVFSYETGVLASKIK 210
Query: 105 EINDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDPLVSPFSERTKGIEVDGCKRN 164
DL SR+ + R VL+G ++ + + ++ G E + N
Sbjct: 211 AYEDLGFSRLFLSKLIVCSPR---------VLMGHTNIELVQVVKTLQSLGFEFEWVMEN 261
Query: 165 VSD 167
+SD
Sbjct: 262 LSD 264
>At1g20930 putative cell division control protein, cdc2 kinase
Length = 315
Score = 28.5 bits (62), Expect = 2.5
Identities = 16/51 (31%), Positives = 25/51 (48%), Gaps = 4/51 (7%)
Query: 44 KKSGYNTPAPMWKTSSKSPSMATHHHHAMKKTALHSSRTGLPFKEKKEISV 94
+K G T +++ K+ M A+KKT LH G+P +EIS+
Sbjct: 20 EKVGEGTYGKVYRAREKATGMIV----ALKKTRLHEDEEGVPPTTLREISI 66
>At5g65470 unknown protein
Length = 504
Score = 28.1 bits (61), Expect = 3.2
Identities = 18/80 (22%), Positives = 38/80 (47%), Gaps = 3/80 (3%)
Query: 55 WKTSSKSPSMATHHHHAMKKTALHSSRTGLPFKEKK---EISVSARKLAATLWEINDLTP 111
W + SP + ++ A+ + S LP + ++ +++ A + + + D
Sbjct: 190 WYIENVSPVLQSYGIAAISPFSHRLSFDHLPAEIQRLRCKVNFQALRFVPHITSLGDALV 249
Query: 112 SRIKKESMKSNKERDKVERL 131
SR++ S +SNKE+ V+ L
Sbjct: 250 SRLRNPSWRSNKEQKNVDHL 269
>At4g18390 teosinte branched1 like protein
Length = 365
Score = 28.1 bits (61), Expect = 3.2
Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 5/49 (10%)
Query: 22 SSTSSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSKSPSMATHHHH 70
+S SSSP F +++G SG T P+ S S++ HHHH
Sbjct: 198 ASAPSSSPMEY---FSSGLILG--SGQQTHFPISTNSHPFSSISDHHHH 241
>At4g09360 putative protein
Length = 853
Score = 28.1 bits (61), Expect = 3.2
Identities = 16/66 (24%), Positives = 33/66 (49%), Gaps = 3/66 (4%)
Query: 69 HHAMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEIND---LTPSRIKKESMKSNKER 125
H+ + ++ GLP+ ++K +S K TLW + L S++K+++ +
Sbjct: 50 HYCFIENVRIFAKNGLPYLQEKLLSEIRGKKQETLWSVEKGCRLIKSKLKEKNFLVLDDV 109
Query: 126 DKVERL 131
D V++L
Sbjct: 110 DNVDQL 115
>At4g01350 putative CHP-rich zinc finger protein
Length = 652
Score = 28.1 bits (61), Expect = 3.2
Identities = 23/92 (25%), Positives = 37/92 (40%), Gaps = 2/92 (2%)
Query: 77 LHSSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVL 136
LH LP K++ L ++ D T I + + V+ C S L
Sbjct: 415 LHEKCANLPMKKRLVFGTRPYTLMKETTQLTDCTLCGIFSDGFAYSSRWLDVDVHCGS-L 473
Query: 137 LGPQKLDPLVSPFS-ERTKGIEVDGCKRNVSD 167
P D + P + ++ +G DGCK+N+ D
Sbjct: 474 NEPLVHDGHIHPLNFDKMEGHFCDGCKKNIDD 505
>At3g19440 unknown protein
Length = 477
Score = 28.1 bits (61), Expect = 3.2
Identities = 19/70 (27%), Positives = 35/70 (49%), Gaps = 3/70 (4%)
Query: 106 INDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDP---LVSPFSERTKGIEVDGCK 162
I++L S +K + +S + +++R C +L+ + L S F E+T G G K
Sbjct: 213 IDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGVK 272
Query: 163 RNVSDLSHQF 172
+NV L ++
Sbjct: 273 KNVKSLKRKY 282
>At2g25000 putative WRKY-type DNA binding protein
Length = 271
Score = 28.1 bits (61), Expect = 3.2
Identities = 16/64 (25%), Positives = 28/64 (43%)
Query: 89 KKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDPLVSP 148
K+E+S SA K+ W + D + ++ E + N E K+ + V L+ L+
Sbjct: 20 KREVSASASKVVEKKWLVKDEKRNMLQDEINRVNSENKKLTEMLARVCEKYYALNNLMEE 79
Query: 149 FSER 152
R
Sbjct: 80 LQSR 83
>At5g35450 disease resistance protein
Length = 901
Score = 27.7 bits (60), Expect = 4.2
Identities = 20/51 (39%), Positives = 24/51 (46%), Gaps = 2/51 (3%)
Query: 135 VLLGPQKLDPLVSPFSERTKGI--EVDGCKRNVSDLSHQFHFVDPYFRGMD 183
V G QKL L++ SER GI +VDG KR + L D G D
Sbjct: 6 VSFGVQKLWALLNRESERLNGIDEQVDGLKRQLRGLQSLLKDADAKKHGSD 56
>At2g42470 hypothetical protein
Length = 898
Score = 27.7 bits (60), Expect = 4.2
Identities = 27/100 (27%), Positives = 45/100 (45%), Gaps = 9/100 (9%)
Query: 60 KSPSMATHH---HHAMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIKK 116
K P A H + +KK +++ + K +S+S +L+ E+ LT K
Sbjct: 369 KHPDTAVHFLPKNKLVKKAHMNTLLCLIKTLRKPPLSLSETELSNAYSELTKLTEVGFKL 428
Query: 117 ESMKSNKERDKVERLCRSVLLGPQKLDPLVSPFSERTKGI 156
+ +KS E+ +ER +SV G Q +S + R K I
Sbjct: 429 DWLKSKLEKASLERK-KSVSDGSQ-----ISAYDSRIKQI 462
>At2g16900 unknown protein
Length = 382
Score = 27.7 bits (60), Expect = 4.2
Identities = 39/177 (22%), Positives = 67/177 (37%), Gaps = 27/177 (15%)
Query: 2 SSCQTKHLKQKHNK-IRKRGCSSTSSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSK 60
S C K + + NK +K+G S F + KK+ P+P+ + +
Sbjct: 23 SDCLEKISQGRGNKNSKKQGSKILSLPGSFGK-----------KKTESQPPSPLSTRNYQ 71
Query: 61 SPSMATHHHHAMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIKKESMK 120
+ + T + + + +T +P K S+S+ ++ L ND
Sbjct: 72 NGAANTPKVRQSRPSPVAMKKTPVPEANKSLHSLSSDGISIDLNGQND------------ 119
Query: 121 SNKERDKVERLCRSVLLGPQKLDPLVSPFSERTKGIEVDGCKRNVSDLSHQFHFVDP 177
K E+ R+V L P + P S R +G E G S++S F+ V P
Sbjct: 120 --SFNHKQEKPSRTVPLSPNSMADRGKPLSPRPQGHEHSGKNDTASEIS-LFNVVSP 173
>At1g76540 putative cell division control protein cdc2
Length = 313
Score = 27.7 bits (60), Expect = 4.2
Identities = 17/51 (33%), Positives = 25/51 (48%), Gaps = 4/51 (7%)
Query: 44 KKSGYNTPAPMWKTSSKSPSMATHHHHAMKKTALHSSRTGLPFKEKKEISV 94
+K G T +++ K AT A+KKT LH G+P +EIS+
Sbjct: 18 EKVGEGTYGKVYRAREK----ATGKIVALKKTRLHEDEEGVPSTTLREISI 64
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.128 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,411,887
Number of Sequences: 26719
Number of extensions: 174944
Number of successful extensions: 703
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 25
Number of HSP's that attempted gapping in prelim test: 687
Number of HSP's gapped (non-prelim): 35
length of query: 199
length of database: 11,318,596
effective HSP length: 94
effective length of query: 105
effective length of database: 8,807,010
effective search space: 924736050
effective search space used: 924736050
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146332.8