BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146331.8 + phase: 2 /pseudo
(279 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g13410 unknown protein 276 9e-75
At2g29260 tropinone reductase like protein 33 0.13
At2g35940 putative homeodomain transcription factor 33 0.22
At1g14690 hypothetical protein 31 0.64
At5g43230 putative protein 30 1.1
At1g50180 disease resistance protein, putative 30 1.1
At5g49910 heat shock protein 70 (Hsc70-7) 30 1.9
At5g52910 putative protein 29 3.2
At3g44805 putative protein 29 3.2
At1g11420 hypothetical protein 29 3.2
At5g63540 unknown protein 28 4.2
At5g60630 unknown protein 28 7.1
At4g26150 putative transcription factor 28 7.1
At1g18485 hypothetical protein 28 7.1
At2g18370 putative lipid transfer protein 27 9.3
At1g07570 protein kinase APK1A 27 9.3
>At3g13410 unknown protein
Length = 321
Score = 276 bits (706), Expect = 9e-75
Identities = 144/281 (51%), Positives = 197/281 (69%), Gaps = 6/281 (2%)
Query: 2 SENG-LKESVNYQVISLKDLAKSVLSEAGWSNFLCKGKKFQDPLDLALLFVGGELQSSDL 60
S NG L E+VNYQV+S KDL SV ++ GWSNFLC KK + P+D+AL+F+G EL SSD+
Sbjct: 42 SANGELDEAVNYQVMSAKDLVGSVFTQGGWSNFLCSEKKLEQPVDVALVFIGRELLSSDV 101
Query: 61 SLNKHADSALSYLLKDSFVRSNTSMAFPYVSASEDVNLEDSLVSGFAEACGDDLGIGNVA 120
S +++D AL L + F SN S+AFPY++A E+ +E+ L+SG EAC +++G+ N+
Sbjct: 102 SSKRNSDPALVNTLNNLFTASNFSLAFPYIAAPEEERMENLLLSGLKEACPNNVGVSNIV 161
Query: 121 FLGSCSMGNGNREETAALQSVQAYLTKRKEESHKGKTDLVVFCN-GPQASKNVDSTKSEG 179
F SC + +G ++ + LQS + +L R+E +G+TDLVV C+ G +++ + SE
Sbjct: 162 FSDSCFVEDGTIQKLSDLQSFKDHLLARRETRKEGETDLVVLCSEGSESNSQAGQSHSER 221
Query: 180 EVLSELISSVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTN-STACDGV 238
E EL+SSVE+SG+KY LYVSD Y SY+ LQRFLAE+ GN + +T CD +
Sbjct: 222 ESFLELVSSVEQSGSKYTALYVSD---PYWYTSYKTLQRFLAETAKGNSTPEIATGCDEL 278
Query: 239 CQLKSSLLEGLLVGIVLLIILISGLCCMMGIDSPTRFEAPQ 279
C+ KSSLLEG+LVGIV L+ILISGLCCM GID+PTRFE PQ
Sbjct: 279 CKFKSSLLEGILVGIVFLLILISGLCCMAGIDTPTRFETPQ 319
>At2g29260 tropinone reductase like protein
Length = 322
Score = 33.5 bits (75), Expect = 0.13
Identities = 32/103 (31%), Positives = 50/103 (48%), Gaps = 9/103 (8%)
Query: 174 STKSEGEVLSELISSVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTNST 233
S +S+ E L E +SSV E K +L V+++ +I+ P + F A + STN
Sbjct: 129 SDRSQREALMETVSSVFEG--KLHIL-VNNVGTNIRKP----MVEFTAGEFSTLMSTNFE 181
Query: 234 ACDGVCQLKSSLLEGLLVGIVLLIILISGLCCM--MGIDSPTR 274
+ +CQL LL G V+ I +SG + M + S T+
Sbjct: 182 SVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTK 224
>At2g35940 putative homeodomain transcription factor
Length = 680
Score = 32.7 bits (73), Expect = 0.22
Identities = 22/100 (22%), Positives = 48/100 (48%), Gaps = 2/100 (2%)
Query: 52 GGELQSSDLSLNKHADSALSYLLKDSFVRSNTSMAFPYVSA-SEDVNLEDSLVSGFAEAC 110
G +++ S + ++ L+ ++++ + V+A S+D+N + L S +C
Sbjct: 169 GEDIRVGSGSTGSGVTNGIANLVSSKYLKAAQELLDEVVNADSDDMNAKSQLFSSKKGSC 228
Query: 111 GDDLGIGNVAFLGSCSMGNGNREETAALQSVQAYLTKRKE 150
G+D +G + G+ G+G E A + V+ +R+E
Sbjct: 229 GNDKPVGE-SSAGAGGEGSGGGAEAAGKRPVELGTAERQE 267
>At1g14690 hypothetical protein
Length = 707
Score = 31.2 bits (69), Expect = 0.64
Identities = 25/92 (27%), Positives = 44/92 (47%), Gaps = 7/92 (7%)
Query: 145 LTKRKEESHKGKTDLVVFCNGPQASKNVDSTKSEGEVLSELISSVEESGAKYAVLYVSDI 204
+ K KE+S L+VF + V++ KS+ E+ L AV+Y D
Sbjct: 619 IQKSKEQS------LIVFSEESSFDEMVNNMKSQSELCVILAKIFGSIAVAIAVVYGVDY 672
Query: 205 SRSIQYPSYRDLQRFLAESTTGNGSTNSTACD 236
+R + P + ++ L +S++ NGS++S D
Sbjct: 673 ARKVLLP-FVWKKKDLGDSSSQNGSSDSEDTD 703
>At5g43230 putative protein
Length = 628
Score = 30.4 bits (67), Expect = 1.1
Identities = 34/119 (28%), Positives = 48/119 (39%), Gaps = 12/119 (10%)
Query: 118 NVAFLGSCSMGNGNREETAALQSVQAYLTKRKEESHKGKTDLVVFCNG---PQASKNVDS 174
N LG ++G L+S+Q + K++S K V+ G PQ SK
Sbjct: 92 NTLALGRKTLGMEGIPRLKQLKSIQLHFDGHKDDSSHKKASGVINKVGLITPQDSKT--- 148
Query: 175 TKSEGEVLS-ELISSVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTNS 232
E L EL + A Y+V+ S S + R L RF + GNGS +S
Sbjct: 149 -----ETLEDELKEAAVLEAAIYSVVAEHTSSMSKVHAPARRLARFYLHACKGNGSDHS 202
>At1g50180 disease resistance protein, putative
Length = 857
Score = 30.4 bits (67), Expect = 1.1
Identities = 34/114 (29%), Positives = 49/114 (42%), Gaps = 17/114 (14%)
Query: 46 LALLFVGGELQSSDLSLNKHADSALSYLLKDSFVRSNTSM---------AFPYVSASEDV 96
++L VG E++ L+K A S L + +K+S R S+ +FPYV V
Sbjct: 106 VSLHSVGSEIREITSRLSKIAASMLDFGIKESMGREGLSLSDSLREQRQSFPYVVEHNLV 165
Query: 97 NLEDSLVSGFAEACGDDLGIG--NVAFLGSCSMGNGNREETAALQSVQAYLTKR 148
LE SL E +DL G + C MG G + T A Q + +R
Sbjct: 166 GLEQSL-----EKLVNDLVSGGEKLRVTSICGMG-GLGKTTLAKQIFHHHKVRR 213
>At5g49910 heat shock protein 70 (Hsc70-7)
Length = 718
Score = 29.6 bits (65), Expect = 1.9
Identities = 48/193 (24%), Positives = 79/193 (40%), Gaps = 36/193 (18%)
Query: 104 SGFAEACGDDLGIGNVAFLGSCSMGNGNREETAALQSVQAYLTKRKEESHKGKTDL---- 159
SG GDD V +L S ++E L + L + E + K K +L
Sbjct: 293 SGDTHLGGDDFDKRVVDWLASTF----KKDEGIDLLKDKQALQRLTEAAEKAKIELSSLT 348
Query: 160 ---------VVFCNGPQASKNVDSTKSEG---EVLSELISSVE---ESGAKYAVLYVSDI 204
+GP K++++T + G E+ S+L+ V E+ + A L DI
Sbjct: 349 QTNMSLPFITATADGP---KHIETTLTRGKFEELCSDLLDRVRTPVENSLRDAKLSFKDI 405
Query: 205 SR------SIQYPSYRDLQRFLAESTTGNGSTNSTACDGVCQLKSSLLEGLLVGIVLLII 258
S + P+ +DL R L TG S D V L +++ G+L G V I+
Sbjct: 406 DEVILVGGSTRIPAVQDLVRKL----TGKEPNVSVNPDEVVALGAAVQAGVLSGDVSDIV 461
Query: 259 LISGLCCMMGIDS 271
L+ +G+++
Sbjct: 462 LLDVTPLSLGLET 474
>At5g52910 putative protein
Length = 1141
Score = 28.9 bits (63), Expect = 3.2
Identities = 23/67 (34%), Positives = 33/67 (48%), Gaps = 5/67 (7%)
Query: 142 QAYLTKRKEESHKGKTDLVVFCNGPQASKNVDSTKSEGEVL--SELISSVEESGAKYAVL 199
Q+Y+T+ ES + +TD+ + NGP S D S L EL ++S A A L
Sbjct: 1064 QSYITR---ESRQSETDVHISDNGPSTSLPEDPNLSSDNELEDDELADWGDDSAAGGASL 1120
Query: 200 YVSDISR 206
S +SR
Sbjct: 1121 TQSPLSR 1127
>At3g44805 putative protein
Length = 248
Score = 28.9 bits (63), Expect = 3.2
Identities = 17/67 (25%), Positives = 33/67 (48%), Gaps = 8/67 (11%)
Query: 49 LFVGGELQSSDLSLNKHADSALSYLLKDSFVRSNTSMAFPYVSASEDVNLEDSLVSGFAE 108
L + +++S+ L +H+D AL + K+ +VR++ ++L D+L +
Sbjct: 57 LVLPSQVESAKLIFERHSDMALEFRAKNQYVRTSCMNVL--------LSLIDTLCQSLQD 108
Query: 109 ACGDDLG 115
DDLG
Sbjct: 109 FSIDDLG 115
>At1g11420 hypothetical protein
Length = 604
Score = 28.9 bits (63), Expect = 3.2
Identities = 31/131 (23%), Positives = 56/131 (42%), Gaps = 19/131 (14%)
Query: 128 GNGN---REETAALQSVQAYLTKRKEESHKGKTDLVVFCNGPQASKNVDSTKSEGEVLSE 184
G GN RE+ ++ Y KRK +G L + N K V S+ V+
Sbjct: 339 GFGNESTREKMPEEPKIKYYTRKRK----RGGLKLNSYIN-----KTVLSSDRTPNVVKN 389
Query: 185 LISSVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTNSTACDGVCQLKSS 244
S+ EE+ AK+ ++ + +S + +Y L+ F + S +S + + K
Sbjct: 390 SASNAEENHAKHTIMVLPFAKKSPVWKTYESLEVFKSVS-------HSLHFSPLFETKQD 442
Query: 245 LLEGLLVGIVL 255
EG +G+++
Sbjct: 443 FREGYAIGMMV 453
>At5g63540 unknown protein
Length = 602
Score = 28.5 bits (62), Expect = 4.2
Identities = 31/112 (27%), Positives = 47/112 (41%), Gaps = 21/112 (18%)
Query: 126 SMGNGNREETAALQSVQAYLTKRKEESHKGKTDLVVFCNGPQASKNVDSTKSEGEVLSEL 185
S +GN T A Q + ++T+ S + P AS NV +T S E +
Sbjct: 280 SSASGNASHTQANQGIPVHVTRTHNSSRA--------MDEPLASTNVGATVSRVEHMQ-- 329
Query: 186 ISSVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTNSTACDG 237
I + G + SDI + + ++ R A ST G G++ S AC G
Sbjct: 330 IDTASAHGERT----FSDI-----HSTSSNIHR--AASTAGTGTSCSGACSG 370
>At5g60630 unknown protein
Length = 139
Score = 27.7 bits (60), Expect = 7.1
Identities = 29/113 (25%), Positives = 49/113 (42%), Gaps = 9/113 (7%)
Query: 46 LALLFVGGELQSSDLSLNKHADSALSYLLKDSFVRSNTSMAFPYVSASE-----DVNLED 100
+ALL V GE+ + ++S S+L + S + Y S+S+ D+N D
Sbjct: 16 VALLMVSGEISTEEISPAIEHSSSLPQSETEMSPSPTMSNDYDYPSSSQLTESNDLNYTD 75
Query: 101 SLVSGFAEA-CGDDLGIGNVAFLGSCSMGNGNREETAALQSVQAYLTKRKEES 152
S G EA G + G G G +G+ A++ Y+ K++ E+
Sbjct: 76 STRPGGEEASVGGENGGGGGKKTGIAVVGS---IAAASMVGFGGYVLKKRREN 125
>At4g26150 putative transcription factor
Length = 352
Score = 27.7 bits (60), Expect = 7.1
Identities = 27/105 (25%), Positives = 48/105 (45%), Gaps = 14/105 (13%)
Query: 128 GNGNREETAALQSVQAYLTKRKEESHKGKTDLVVFCNGPQAS-KNVDSTKSEGEVLSELI 186
G+ + ++ + + LT +K+++H+ +TDL PQ+ K++ T S L +
Sbjct: 103 GSSSSDQMVPKKETRLKLTIKKKDNHQDQTDL------PQSPIKDMTGTNS----LKWIS 152
Query: 187 SSVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTN 231
S V K A++ SD S+ + D L+ S NG N
Sbjct: 153 SKVRLMKKKKAIITTSDSSKQ---HTNNDQSSNLSNSERQNGYNN 194
>At1g18485 hypothetical protein
Length = 970
Score = 27.7 bits (60), Expect = 7.1
Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Query: 51 VGGELQSSD--LSLNKHADSALSYLLKDSF 78
+GG QS+D LSL+ H +S LL DSF
Sbjct: 468 IGGHAQSNDPRLSLDAHLQMKISGLLPDSF 497
>At2g18370 putative lipid transfer protein
Length = 116
Score = 27.3 bits (59), Expect = 9.3
Identities = 12/31 (38%), Positives = 20/31 (63%)
Query: 214 RDLQRFLAESTTGNGSTNSTACDGVCQLKSS 244
+DLQ ++ T+G+G+ T CDGV L ++
Sbjct: 33 QDLQPCVSYLTSGSGNPPETCCDGVKSLAAA 63
>At1g07570 protein kinase APK1A
Length = 410
Score = 27.3 bits (59), Expect = 9.3
Identities = 14/47 (29%), Positives = 25/47 (52%), Gaps = 4/47 (8%)
Query: 51 VGGELQSSDLSLNKHADSALSYLLKDSFVR----SNTSMAFPYVSAS 93
+GG + +D + + +DS +S + F R +T +A+P SAS
Sbjct: 360 IGGNMDKTDRRMRRRSDSVVSKKVNAGFARQTAVGSTVVAYPRPSAS 406
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.313 0.131 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,778,024
Number of Sequences: 26719
Number of extensions: 231278
Number of successful extensions: 561
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 556
Number of HSP's gapped (non-prelim): 16
length of query: 279
length of database: 11,318,596
effective HSP length: 98
effective length of query: 181
effective length of database: 8,700,134
effective search space: 1574724254
effective search space used: 1574724254
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146331.8