BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146331.7 - phase: 0 
         (377 letters)

Database: ara_mips 
           26,719 sequences; 11,318,596 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

At2g43360 biotin synthase (Bio B)                                     615  e-177
At2g31955 molybdopterin synthase (CNX2)                                38  0.008
At2g16640 putative chloroplast outer membrane protein                  29  3.7
At1g77410 unknown protein                                              29  3.7
At4g17000 hypothetical protein                                         28  8.2
At3g58390 pelota-like protein                                          28  8.2
At2g25430 unknown protein                                              28  8.2

>At2g43360 biotin synthase (Bio B)
          Length = 378

 Score =  615 bits (1587), Expect = e-177
 Identities = 297/378 (78%), Positives = 342/378 (89%), Gaps = 2/378 (0%)

Query: 1   MFWLRPILRSQSRSSI-WVLQHCNSFSTSSAAAIQAEKTIQNGPRNDWTKDEVKSIYDSP 59
           M  +R + RSQ R S+   LQ  + +S+ SAA+ +AE+TI+ GPRNDW++DE+KS+YDSP
Sbjct: 1   MMLVRSVFRSQLRPSVSGGLQSASCYSSLSAASAEAERTIREGPRNDWSRDEIKSVYDSP 60

Query: 60  ILDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKGQKLLNKD 119
           +LDLLFHGAQVHRH HNFREVQQCTLLS+KTGGCSEDCSYCPQSSRY TG+K Q+L++KD
Sbjct: 61  LLDLLFHGAQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYSTGVKAQRLMSKD 120

Query: 120 AVLQAAVKAKEAGSTRFCMGAAWRDTIGRKTNFNQILEYVKEIKGMGMEVCCTLGMLDKD 179
           AV+ AA KAKEAGSTRFCMGAAWRDTIGRKTNF+QILEY+KEI+GMGMEVCCTLGM++K 
Sbjct: 121 AVIDAAKKAKEAGSTRFCMGAAWRDTIGRKTNFSQILEYIKEIRGMGMEVCCTLGMIEKQ 180

Query: 180 QAGELKKAGLTAYNHNLDTSREYYPNIITTRTYDERLKTLEFVRDAGINVCSGGIIGLGE 239
           QA ELKKAGLTAYNHNLDTSREYYPN+ITTR+YD+RL+TL  VRDAGINVCSGGIIGLGE
Sbjct: 181 QALELKKAGLTAYNHNLDTSREYYPNVITTRSYDDRLETLSHVRDAGINVCSGGIIGLGE 240

Query: 240 AEDDRVGLLHTLSTLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKA 299
           AE+DR+GLLHTL+TLP+HPESVPINAL+AVKGTPL+DQKPVEIWEMIRMI TARI MPKA
Sbjct: 241 AEEDRIGLLHTLATLPSHPESVPINALLAVKGTPLEDQKPVEIWEMIRMIGTARIVMPKA 300

Query: 300 MVRLSAGRVRFSVPEQALCFLAGANSIFAGEKLLTTANNDFDADQLMFKVLGLLPKAPSL 359
           MVRLSAGRVRFS+ EQALCFLAGANSIF GEKLLTT NNDFDADQLMFK LGL+PK PS 
Sbjct: 301 MVRLSAGRVRFSMSEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKTLGLIPKPPSF 360

Query: 360 DDDETNEAENYKEAASSS 377
            +D+ +E+EN ++ AS+S
Sbjct: 361 SEDD-SESENCEKVASAS 377


>At2g31955 molybdopterin synthase (CNX2)
          Length = 390

 Score = 38.1 bits (87), Expect = 0.008
 Identities = 46/205 (22%), Positives = 83/205 (40%), Gaps = 21/205 (10%)

Query: 45  NDWTKDEVKSIYDSPILDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSS 104
           + +   +V  I D+P+ D+L            F  +     +S+ T  C+  C YC  S 
Sbjct: 47  SSYAAHQVDQIKDNPVSDMLID---------KFGRLHTYLRISL-TERCNLRCQYCMPSE 96

Query: 105 RYDTGLKGQKLLNKDAVLQAAVKAKEAGSTRFCMGAAWRDTIGRKT---NFNQILEYVKE 161
             +   K Q LL++  +++ A     AG  +       R T G  T   +  +I   +  
Sbjct: 97  GVELTPKPQ-LLSQSEIVRLAGLFVSAGVNKI------RLTGGEPTVRKDIEEICLQLSS 149

Query: 162 IKGMGMEVCCTLGMLDKDQAGELKKAGLTAYNHNLDTSREYYPNIITTRT-YDERLKTLE 220
           +KG+      T G+    +   LK+ GL + N +LDT        +T R  +D  +K+++
Sbjct: 150 LKGLKNLAITTNGITLAKKLPRLKECGLDSLNISLDTLVPAKFEFLTRRKGHDRVMKSID 209

Query: 221 FVRDAGINVCSGGIIGLGEAEDDRV 245
              + G N      + +    DD +
Sbjct: 210 TAIELGYNPVKVNCVIMRGLNDDEI 234


>At2g16640 putative chloroplast outer membrane protein
          Length = 1206

 Score = 29.3 bits (64), Expect = 3.7
 Identities = 24/90 (26%), Positives = 40/90 (43%), Gaps = 7/90 (7%)

Query: 195 NLDTSREYYPNIITTRTYDERLKTLEFVRDAGINVCSGGIIGLGEAEDDRVGLL---HTL 251
           N +T   Y  NI+T  + D  + +   V  + +   S G    GE E D   L    H  
Sbjct: 303 NGETGAAYTSNIVTNASGDNEVSSA--VTSSPLEESSSG--EKGETEGDSTCLKPEQHLA 358

Query: 252 STLPTHPESVPINALIAVKGTPLQDQKPVE 281
           S+  ++PES  +++     G   ++ KPV+
Sbjct: 359 SSPHSYPESTEVHSNSGSPGVTSREHKPVQ 388


>At1g77410 unknown protein
          Length = 815

 Score = 29.3 bits (64), Expect = 3.7
 Identities = 24/98 (24%), Positives = 43/98 (43%), Gaps = 20/98 (20%)

Query: 155 ILEYVKEIKGMGMEVCCTLGMLDKDQAGELKKAGLTAYNHNLDTSREYYPNIITTRTYDE 214
           I++++KE+K  G+ VC  +G   +   GE    GL  + HN+          I  RT +E
Sbjct: 95  IVKFIKEVKNHGLYVCLRIGPFIQ---GEWSYGGLPFWLHNVQG--------IVFRTDNE 143

Query: 215 RLK---------TLEFVRDAGINVCSGGIIGLGEAEDD 243
             K          ++ ++   +    GG I L + E++
Sbjct: 144 PFKYHMKRYAKMIVKLMKSENLYASQGGPIILSQIENE 181


>At4g17000 hypothetical protein
          Length = 674

 Score = 28.1 bits (61), Expect = 8.2
 Identities = 16/66 (24%), Positives = 32/66 (48%), Gaps = 1/66 (1%)

Query: 252 STLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGRVRFS 311
           +T+P  P    ++ L  +   P +    +   E +++++T +   PKA  +     V  S
Sbjct: 98  NTVPAKPSRSRVSKLAMISSIPQKGNGNIRSKE-VKVVSTNKNVTPKAKAKGKESAVISS 156

Query: 312 VPEQAL 317
           VP++AL
Sbjct: 157 VPQKAL 162


>At3g58390 pelota-like protein
          Length = 395

 Score = 28.1 bits (61), Expect = 8.2
 Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 3/54 (5%)

Query: 4   LRPILRSQSRSSIWVLQHCNSFSTSSAAAIQAEKTIQNGPRNDWTKDEVKSIYD 57
           LRPIL ++SR   ++L H NS    S + +  +  + N  ++     EVK++ D
Sbjct: 247 LRPILENKSR---FILVHTNSGYKHSLSEVLHDPNVMNMIKDTKAAKEVKALND 297


>At2g25430 unknown protein
          Length = 653

 Score = 28.1 bits (61), Expect = 8.2
 Identities = 17/41 (41%), Positives = 22/41 (53%), Gaps = 4/41 (9%)

Query: 210 RTY----DERLKTLEFVRDAGINVCSGGIIGLGEAEDDRVG 246
           RTY    D+RL+   F R +G++V SGG        DDR G
Sbjct: 139 RTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRYG 179


  Database: ara_mips
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,978,382
  Number of sequences in database:  6832
  
  Database: /data/blast2/ara_mips_chr2
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,737,135
  Number of sequences in database:  4184
  
  Database: /data/blast2/ara_mips_chr3
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,236,886
  Number of sequences in database:  5377
  
  Database: /data/blast2/ara_mips_chr4
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,748,816
  Number of sequences in database:  4030
  
  Database: /data/blast2/ara_mips_chr5
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,569,679
  Number of sequences in database:  6098
  
  Database: /data/blast2/ara_mips_chl
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 25,951
  Number of sequences in database:  85
  
  Database: /data/blast2/ara_mips_mit
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 21,747
  Number of sequences in database:  113
  
Lambda     K      H
   0.318    0.133    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,289,467
Number of Sequences: 26719
Number of extensions: 343492
Number of successful extensions: 745
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 744
Number of HSP's gapped (non-prelim): 7
length of query: 377
length of database: 11,318,596
effective HSP length: 101
effective length of query: 276
effective length of database: 8,619,977
effective search space: 2379113652
effective search space used: 2379113652
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)