BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146329.8 + phase: 0
(402 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44520 heme A farnesyltransferase like protein 482 e-136
At4g23660 para-hydroxy bezoate polyprenyl diphosphate transferas... 52 4e-07
At2g03590 unknown protein 33 0.36
At2g16640 putative chloroplast outer membrane protein 32 0.62
At3g16620 putative GTP-binding protein 30 1.8
At3g51820 chlorophyll synthetase like protein 30 2.3
At1g23090 putative sulphate transporter protein 30 3.1
At1g15160 hypothetical protein 30 3.1
At3g27880 unknown protein 29 4.0
cox1 -mitochondrial genome- cytochrome c oxidase subunit 1 29 5.2
At4g29380 unknown protein 29 5.2
At3g60200 unknown protein (At3g60200) 29 5.2
At3g12620 unknown protein 29 5.2
At5g35210 putative protein 28 6.8
At3g13150 hypothetical protein 28 6.8
At2g24430 NAM (no apical meristem)-like protein 28 6.8
At2g15820 unknown protein 28 6.8
At1g17040 hypothetical protein 28 6.8
At2g45150 putative phosphatidate cytidylyltransferase (T14P1.4/A... 28 8.9
At2g40070 En/Spm-like transposon protein 28 8.9
>At2g44520 heme A farnesyltransferase like protein
Length = 431
Score = 482 bits (1240), Expect = e-136
Identities = 242/370 (65%), Positives = 291/370 (78%), Gaps = 14/370 (3%)
Query: 32 STFSSSPSVSASKSNDLISLARHYGNCYSQLSKARLSLLVVATSGTGFVLGSGGA-VDLS 90
+T +++ +S+ L L HY CY +LSKA+LS+LVVATSGTG++LG+G A +
Sbjct: 71 ATATATTGEISSRVAALAGLGHHYARCYWELSKAKLSMLVVATSGTGYILGTGNAAISFP 130
Query: 91 MLSYTCLGTMMVAASASTLNQVFEIKNDAIMRRTSQRPLPSGRITVPHAVGWASSVGLAG 150
L YTC GTMM+AASA++LNQ+FEI ND+ M+RT RPLPSGRI+VPHAV WA+ G +G
Sbjct: 131 GLCYTCAGTMMIAASANSLNQIFEISNDSKMKRTMLRPLPSGRISVPHAVAWATIAGASG 190
Query: 151 TALLATQTNMLAAGLAASNLVLYSFVYTPLKQIHPVNTWVGAVVGAIPPLLGWAAASGDI 210
LLA++TNMLAAGLA++NLVLY+FVYTPLKQ+HP+NTWVGAVVGAIPPLLGWAAASG I
Sbjct: 191 ACLLASKTNMLAAGLASANLVLYAFVYTPLKQLHPINTWVGAVVGAIPPLLGWAAASGQI 250
Query: 211 SLNSLILPAALYFWQIPHFMALAYMCREDYAAGGFKMYSLADPSGRKTAMVAMRNSIYLI 270
S NS+ILPAALYFWQIPHFMALA++CR DYAAGG+KM SL DPSG++ A VA+RN Y+I
Sbjct: 251 SYNSMILPAALYFWQIPHFMALAHLCRNDYAAGGYKMLSLFDPSGKRIAAVALRNCFYMI 310
Query: 271 PLGFLAYDWGVTSGWFCLESTALTLAISAAAFSFYRDRTKERARRMFHASLLYLPVFMAG 330
PLGF+AYDWG+TS WFCLEST LTLAI+A AFSFYRDRT +AR+MFHASLL+LPVFM+G
Sbjct: 311 PLGFIAYDWGLTSSWFCLESTLLTLAIAATAFSFYRDRTMHKARKMFHASLLFLPVFMSG 370
Query: 331 LLIHR-RTDNQHFLEVNAEGFMKSPSSVETSEMDDKNGNQKIKGRQGTRARPPVAYASIA 389
LL+HR DNQ L V G S S G K + R+ A+PPVAYAS A
Sbjct: 371 LLLHRVSNDNQQQL-VEEAGLTNSVS-----------GEVKTQRRKKRVAQPPVAYASAA 418
Query: 390 PFPFLPAPSY 399
PFPFLPAPS+
Sbjct: 419 PFPFLPAPSF 428
>At4g23660 para-hydroxy bezoate polyprenyl diphosphate transferase
(AtPPT1)
Length = 407
Score = 52.4 bits (124), Expect = 4e-07
Identities = 53/194 (27%), Positives = 84/194 (42%), Gaps = 16/194 (8%)
Query: 98 GTMMVAASASTLNQVFEIKNDAIMRRTSQRPLPSGRITVPHAVGWASSVGLAGTALLATQ 157
G +++ + T+N + + D + RT RP+ SG +T +G+ L G +L Q
Sbjct: 171 GALLLRGAGCTINDLLDQDIDTKVDRTKLRPIASGLLTPFQGIGFLGLQLLLGLGIL-LQ 229
Query: 158 TNMLAAGLAASNLVLYSFVYTPLKQIHPVNTWVGAVVGAI---PPLLGWAAASGDISLNS 214
N + L AS+L+L F Y +K+ W A +G LLGW A G I+ S
Sbjct: 230 LNNYSRVLGASSLLLV-FSYPLMKRF---TFWPQAFLGLTINWGALLGWTAVKGSIA-PS 284
Query: 215 LILPAAL--YFWQIPHFMALAYMCREDYAAGGFKMYSLADPSGRKTAMVAMRNSIYLIPL 272
++LP L W + + A+ +ED G K +L K + + +
Sbjct: 285 IVLPLYLSGVCWTLVYDTIYAHQDKEDDVKVGVKSTALRFGDNTKLWLTGFGTA----SI 340
Query: 273 GFLAYD-WGVTSGW 285
GFLA + GW
Sbjct: 341 GFLALSGFSADLGW 354
>At2g03590 unknown protein
Length = 390
Score = 32.7 bits (73), Expect = 0.36
Identities = 30/125 (24%), Positives = 49/125 (39%), Gaps = 3/125 (2%)
Query: 257 KTAMVAMRNSIYLIPLGFLA--YDWGVTSGWFCLESTALTLAISAAAFSFYRDRTKERAR 314
++ M AM I L LG LA Y W TA + +++ D RA
Sbjct: 80 QSVMFAMAGGIVL-SLGNLATQYAWAYVGLSVTEVITASITVVIGTTLNYFLDDRINRAE 138
Query: 315 RMFHASLLYLPVFMAGLLIHRRTDNQHFLEVNAEGFMKSPSSVETSEMDDKNGNQKIKGR 374
+F +L G +H+ + ++ +++ SS E + NG K K +
Sbjct: 139 VLFPGVACFLIAVCFGSAVHKSNAADNKTKLQNFKSLETTSSFEMETISASNGLTKGKAK 198
Query: 375 QGTRA 379
+GT A
Sbjct: 199 EGTAA 203
>At2g16640 putative chloroplast outer membrane protein
Length = 1206
Score = 32.0 bits (71), Expect = 0.62
Identities = 27/88 (30%), Positives = 40/88 (44%), Gaps = 5/88 (5%)
Query: 103 AASASTLNQVFEIKNDAIMR--RTSQRPLPSGRITVPH--AVGWASSVGLAGTALLATQT 158
A +N V ++N + R R QR LP+G++ PH + +AS + ALL Q
Sbjct: 736 AGDMRLMNPVSLVENHSACRTNRAGQRVLPNGQVWKPHLLLLSFASKILAEANALLKLQD 795
Query: 159 NMLAAGLAA-SNLVLYSFVYTPLKQIHP 185
N+ AA S F+ + L Q P
Sbjct: 796 NIPGRPFAARSKAPPLPFLLSSLLQSRP 823
>At3g16620 putative GTP-binding protein
Length = 1089
Score = 30.4 bits (67), Expect = 1.8
Identities = 20/62 (32%), Positives = 31/62 (49%), Gaps = 4/62 (6%)
Query: 103 AASASTLNQVFEIKNDAIMR--RTSQRPLPSGRITVPH--AVGWASSVGLAGTALLATQT 158
A +N V ++N + R R QR LP+G++ PH + +AS + ALL Q
Sbjct: 618 AGDMRLMNPVSLVENHSACRTNRAGQRVLPNGQVWKPHLLLLSFASKILAEANALLKLQD 677
Query: 159 NM 160
N+
Sbjct: 678 NI 679
>At3g51820 chlorophyll synthetase like protein
Length = 387
Score = 30.0 bits (66), Expect = 2.3
Identities = 61/255 (23%), Positives = 93/255 (35%), Gaps = 26/255 (10%)
Query: 91 MLSYTCLGTMMVAASASTLNQVFEIKNDAIMRRTSQRPLPSGRITVPHAVG--WASSVGL 148
M+S CL T+N ++ DAI RP+PSG I+ P + W +G
Sbjct: 141 MMSGPCL-----TGYTQTINDWYDRDIDAI--NEPYRPIPSGAISEPEVITQVWVLLLGG 193
Query: 149 AGTA----LLATQTNMLAAGLA-ASNLVLYSFVYTPLKQIHPVNTWVG-AVVGAIPPLLG 202
G A + A T LA +L+ Y + PLK N WVG +GA L
Sbjct: 194 LGIAGILDVWAGHTTPTVFYLALGGSLLSYIYSAPPLKL--KQNGWVGNFALGASYISLP 251
Query: 203 WAAASGDISLNSLILPAALYFWQIPHFMALAYMCREDYAA----GGFKMYSLADPSGRKT 258
W A +L + P + + L D+ + + SL G +T
Sbjct: 252 WWAGQ---ALFGTLTPDVVVLTLLYSIAGLGIAIVNDFKSVEGDRALGLQSLPVAFGTET 308
Query: 259 AMVAMRNSIYLIPLGFLAYDWGVTSGWFCLESTALTLAISAAAFSFY-RDRTKERARRMF 317
A +I + L Y ++ L AL + F ++ +D K +
Sbjct: 309 AKWICVGAIDITQLSVAGYLLASGKPYYALALVALIIPQIVFQFKYFLKDPVKYDVKYQA 368
Query: 318 HAS-LLYLPVFMAGL 331
A L L +F+ L
Sbjct: 369 SAQPFLVLGIFVTAL 383
>At1g23090 putative sulphate transporter protein
Length = 631
Score = 29.6 bits (65), Expect = 3.1
Identities = 30/105 (28%), Positives = 44/105 (41%), Gaps = 15/105 (14%)
Query: 139 AVGWASSVGLAGTALLATQT-------NMLAAGLAASNLVLYSFVYTPLKQIHPVNTWVG 191
A+G + VG A + + T N A A SN+V+ V L + P+ +
Sbjct: 350 AIGLMNVVGSATSCYVTTGAFSRSAVNNNAGAKTAVSNIVMSVTVMVTLLFLMPLFEYTP 409
Query: 192 AVVGAIPPLLGWAAASGDISLNSLILPAALYFWQIPHFMALAYMC 236
VV LG + I L + LPAA + W+I F L +C
Sbjct: 410 NVV------LGAIIVTAVIGL--IDLPAACHIWKIDKFDFLVMLC 446
>At1g15160 hypothetical protein
Length = 487
Score = 29.6 bits (65), Expect = 3.1
Identities = 38/155 (24%), Positives = 59/155 (37%), Gaps = 27/155 (17%)
Query: 86 AVDLSMLSYTCLGTMMVAASASTLNQVFEIKNDAIMRRTSQRPLPSGRITVPHAVGWAS- 144
+V L LS T + +AA+AST I N+ L +G H V +A+
Sbjct: 295 SVCLQTLSMTYSIPLAIAAAAST-----RISNE----------LGAGNSRAAHIVVYAAM 339
Query: 145 -----SVGLAGTALLATQTNMLAAGLAASNLVLYSFVYTPLKQIHPVNTWVGAVVGAIPP 199
+ GT+LLA + + + N + Y PL I + + V+ +
Sbjct: 340 SLAVVDALMVGTSLLAGKNLLGQVFSSDKNTIDYVAKMAPLVSISLILDSLQGVLSGVAS 399
Query: 200 LLGWAAASGDISLNSLILPAALYFWQIPHFMALAY 234
GW I+ A Y W IP +LA+
Sbjct: 400 GCGWQHIGAYINFG------AFYLWGIPIAASLAF 428
>At3g27880 unknown protein
Length = 242
Score = 29.3 bits (64), Expect = 4.0
Identities = 25/97 (25%), Positives = 40/97 (40%), Gaps = 13/97 (13%)
Query: 32 STFSSSPSVSASKSNDLISLARHYGNCYSQLSKARLSLLVVATSGTGFVLGSGGAVDLSM 91
+T S SPS S + L+ +A CY + S A ATS + G GG V
Sbjct: 6 ATASFSPSFSTYSGDRLVEIAERV--CYKEKSDAEFEFSTAATSSS----GDGGLV---- 55
Query: 92 LSYTCLGTMMVAASASTLNQVFEIKNDAIMRRTSQRP 128
+ +++ S +V +K+ + R Q+P
Sbjct: 56 --FPVFNKNLISGDVSP-EKVIPLKDLFLRERNDQQP 89
>cox1 -mitochondrial genome- cytochrome c oxidase subunit 1
Length = 527
Score = 28.9 bits (63), Expect = 5.2
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 9/58 (15%)
Query: 68 SLLVVATSGTGFVL---------GSGGAVDLSMLSYTCLGTMMVAASASTLNQVFEIK 116
S LV SGTG+ + SGGAVDL++ S G + S + + +F ++
Sbjct: 118 SALVEVGSGTGWTVYPPLSGITSHSGGAVDLAIFSLHLSGVSSILGSINFITTIFNMR 175
>At4g29380 unknown protein
Length = 1494
Score = 28.9 bits (63), Expect = 5.2
Identities = 20/79 (25%), Positives = 39/79 (49%), Gaps = 2/79 (2%)
Query: 303 SFYRDRTKERARRMFHASLLYLPVFMAGLLIH--RRTDNQHFLEVNAEGFMKSPSSVETS 360
S + T+E R + +++ L + G LIH + +D E+N++ +P+S S
Sbjct: 535 SMLPEDTEESVRICYASNIAKLALTAYGFLIHSFQLSDVGVLNELNSQQISTTPASETPS 594
Query: 361 EMDDKNGNQKIKGRQGTRA 379
+ NGN +++ + T A
Sbjct: 595 HLQKANGNAQLQQLRKTIA 613
>At3g60200 unknown protein (At3g60200)
Length = 305
Score = 28.9 bits (63), Expect = 5.2
Identities = 15/52 (28%), Positives = 25/52 (47%)
Query: 287 CLESTALTLAISAAAFSFYRDRTKERARRMFHASLLYLPVFMAGLLIHRRTD 338
CL L+LA SAA + D +++ H LL P ++ + R++D
Sbjct: 20 CLRERLLSLAASAAVSAAVEDNQSKKSNNNNHPPLLIFPRSVSPYVTRRKSD 71
>At3g12620 unknown protein
Length = 385
Score = 28.9 bits (63), Expect = 5.2
Identities = 13/43 (30%), Positives = 22/43 (50%)
Query: 314 RRMFHASLLYLPVFMAGLLIHRRTDNQHFLEVNAEGFMKSPSS 356
RR FH + + VF+ L+ R T + L ++ G + PS+
Sbjct: 343 RRHFHDDITVIVVFLDSHLVSRSTSRRPLLSISGGGDLAGPST 385
>At5g35210 putative protein
Length = 1606
Score = 28.5 bits (62), Expect = 6.8
Identities = 22/72 (30%), Positives = 30/72 (41%), Gaps = 4/72 (5%)
Query: 330 GLLIHRRTDNQHF----LEVNAEGFMKSPSSVETSEMDDKNGNQKIKGRQGTRARPPVAY 385
GL T N H+ L V+A + SS ET E D + N K A+
Sbjct: 643 GLSFKPNTYNNHYTNGELAVSAAASLAVLSSEETHEPDLRKYNSAKKAASSNILEQMKAF 702
Query: 386 ASIAPFPFLPAP 397
+ +AP F P+P
Sbjct: 703 SLVAPRFFWPSP 714
>At3g13150 hypothetical protein
Length = 551
Score = 28.5 bits (62), Expect = 6.8
Identities = 20/43 (46%), Positives = 27/43 (62%), Gaps = 2/43 (4%)
Query: 13 SRLVNSS--SSSSLHNFTHIRSTFSSSPSVSASKSNDLISLAR 53
S V+SS SSSS+ + S+FSSSP S+S S+ L S +R
Sbjct: 461 SSSVSSSPDSSSSVSSSPDSYSSFSSSPDSSSSVSSSLFSSSR 503
>At2g24430 NAM (no apical meristem)-like protein
Length = 316
Score = 28.5 bits (62), Expect = 6.8
Identities = 14/37 (37%), Positives = 24/37 (64%)
Query: 18 SSSSSSLHNFTHIRSTFSSSPSVSASKSNDLISLARH 54
SSSS+S H+ H R++ S+ + + + S+DL+ L H
Sbjct: 176 SSSSTSHHHNNHTRASILSTNNNNPNYSSDLLQLPPH 212
>At2g15820 unknown protein
Length = 849
Score = 28.5 bits (62), Expect = 6.8
Identities = 25/66 (37%), Positives = 35/66 (52%), Gaps = 5/66 (7%)
Query: 18 SSSSSSLHNFTHIRSTFSSSPSVSASKSNDL----ISLARHYGNCYSQLSKARLSLLVVA 73
SSSSS++ T S+ SS+P++ S S SL RH + YS+ S RLS+ V
Sbjct: 27 SSSSSTVSVTTFNISSLSSNPNIINSSSTLFRSLSFSLIRHRSS-YSRRSLRRLSIHTVH 85
Query: 74 TSGTGF 79
+ T F
Sbjct: 86 GNKTQF 91
>At1g17040 hypothetical protein
Length = 641
Score = 28.5 bits (62), Expect = 6.8
Identities = 23/71 (32%), Positives = 30/71 (41%), Gaps = 13/71 (18%)
Query: 279 WGVTSGWFCLESTALTLAISAAAFSFYRDRTKERARRMFHASLLYLPVFMAGLLIHRRTD 338
W V SG FC LT AI + ASLLY +G + +R+D
Sbjct: 211 WSVVSGTFCSLDVVLTNAIGQPVHKDVK----------VVASLLYAD---SGTHVEKRSD 257
Query: 339 NQHFLEVNAEG 349
+ FL V+ EG
Sbjct: 258 FEAFLLVSYEG 268
>At2g45150 putative phosphatidate cytidylyltransferase
(T14P1.4/At2g45150)
Length = 430
Score = 28.1 bits (61), Expect = 8.9
Identities = 19/58 (32%), Positives = 25/58 (42%), Gaps = 13/58 (22%)
Query: 145 SVGLAGTAL-----LATQTNMLAAGLAASNLVLYSFVYTPLKQIHPVNTWVGAVVGAI 197
+VGL T + +AT T G +F TPL I P TW G +VG +
Sbjct: 292 TVGLVATLISFSGVIATDTFAFLGGK--------TFGRTPLTSISPKKTWEGTIVGLV 341
>At2g40070 En/Spm-like transposon protein
Length = 510
Score = 28.1 bits (61), Expect = 8.9
Identities = 17/35 (48%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Query: 9 ATLYSRLVNSSSSSSLHNFTHIRSTFSSSPSVSAS 43
ATL SRL NSS+ S+ N R +SSP +S+S
Sbjct: 109 ATLTSRLANSSTESAARNHLTSRQQ-TSSPGLSSS 142
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.132 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,344,605
Number of Sequences: 26719
Number of extensions: 329070
Number of successful extensions: 1167
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1152
Number of HSP's gapped (non-prelim): 24
length of query: 402
length of database: 11,318,596
effective HSP length: 102
effective length of query: 300
effective length of database: 8,593,258
effective search space: 2577977400
effective search space used: 2577977400
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146329.8