BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146329.10 - phase: 0
(68 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53020 ubiquitin-conjugating enzyme like protein 45 5e-06
At5g67340 unknown proteins 26 3.2
At3g51630 MAP kinase like protein 25 5.5
At3g15355 ubiquitin-conjugating like enzyme 25 5.5
At2g42930 hypothetical protein 25 5.5
At2g25180 putative two-component response regulator protein 25 5.5
At4g23440 hypothetical protein 25 9.3
At3g25840 protein kinase like protein 25 9.3
>At1g53020 ubiquitin-conjugating enzyme like protein
Length = 1163
Score = 45.4 bits (106), Expect = 5e-06
Identities = 21/52 (40%), Positives = 31/52 (59%)
Query: 3 AGNAYIEGAQVGCLVKGGVQDLDDGDKSSSRQFNNSLARHINVAAPNFTGIG 54
A NAY GA +G +VKGGVQDL++ +S S++F +A + F +G
Sbjct: 459 ACNAYKAGAPLGSMVKGGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVKLG 510
Score = 44.7 bits (104), Expect = 9e-06
Identities = 21/52 (40%), Positives = 30/52 (57%)
Query: 3 AGNAYIEGAQVGCLVKGGVQDLDDGDKSSSRQFNNSLARHINVAAPNFTGIG 54
A NAY GA +G +VKGGVQDL+ +S S++F +A + F +G
Sbjct: 1079 ACNAYKAGAPLGSMVKGGVQDLEQARQSGSKKFKTDVASFMQTVVDEFVKLG 1130
Score = 27.7 bits (60), Expect = 1.1
Identities = 20/45 (44%), Positives = 24/45 (52%), Gaps = 8/45 (17%)
Query: 3 AGNAYIEGAQVGCLVKGGVQDLDDGDKSSSRQFNNSLARHINVAA 47
A NAY +GA VG + KGGV K +RQ SL INV +
Sbjct: 784 ACNAYKDGAPVGSIDKGGV-------KKQTRQ-RGSLKFRINVTS 820
>At5g67340 unknown proteins
Length = 707
Score = 26.2 bits (56), Expect = 3.2
Identities = 14/47 (29%), Positives = 24/47 (50%), Gaps = 3/47 (6%)
Query: 7 YIEGAQVGCLVKGGVQDLDDGDKSSSRQFNN---SLARHINVAAPNF 50
Y G +G L+K +++L D D + S NN LA++++ F
Sbjct: 38 YTRGEDIGKLIKPVLENLIDSDAAPSELLNNGFEELAQYVDELREQF 84
>At3g51630 MAP kinase like protein
Length = 549
Score = 25.4 bits (54), Expect = 5.5
Identities = 9/18 (50%), Positives = 15/18 (83%)
Query: 21 VQDLDDGDKSSSRQFNNS 38
+ D+DDG++SS+R N+S
Sbjct: 459 IPDMDDGNRSSNRLLNSS 476
>At3g15355 ubiquitin-conjugating like enzyme
Length = 259
Score = 25.4 bits (54), Expect = 5.5
Identities = 15/41 (36%), Positives = 20/41 (48%)
Query: 3 AGNAYIEGAQVGCLVKGGVQDLDDGDKSSSRQFNNSLARHI 43
A NAY GA G LVKG ++ SS +F +A +
Sbjct: 170 ACNAYRNGATPGYLVKGAPDVEENSAGMSSLKFRTDVATFV 210
>At2g42930 hypothetical protein
Length = 134
Score = 25.4 bits (54), Expect = 5.5
Identities = 18/53 (33%), Positives = 25/53 (46%), Gaps = 1/53 (1%)
Query: 10 GAQVGCLVKGG-VQDLDDGDKSSSRQFNNSLARHINVAAPNFTGIGSMLVQAP 61
GA L KGG QDLD+ +S FN+ ++ +F G + VQ P
Sbjct: 63 GADCSPLEKGGRCQDLDNYRSQASYAFNDYYQKNPIPRNCDFNGAAVLTVQDP 115
>At2g25180 putative two-component response regulator protein
Length = 573
Score = 25.4 bits (54), Expect = 5.5
Identities = 14/43 (32%), Positives = 26/43 (59%), Gaps = 1/43 (2%)
Query: 26 DGDKSSSRQFNNSLARHINVAAPNFTGIGSMLVQAPDQNIRVL 68
+ +KS + N S+A ++A P+F+ + L+ AP+ N+ VL
Sbjct: 371 NNNKSRNMNSNKSIAG-TSMAFPSFSTQQNSLISAPNNNVVVL 412
>At4g23440 hypothetical protein
Length = 964
Score = 24.6 bits (52), Expect = 9.3
Identities = 15/63 (23%), Positives = 29/63 (45%), Gaps = 4/63 (6%)
Query: 6 AYIEGAQVGCLVKGGVQDLDDGDKSSSRQFNNSLARHINVAAPNFTGIGSMLVQAPDQNI 65
+Y+ GA+ L++G V+D + + R + L R +G++L + P
Sbjct: 568 SYLSGAEAMSLMQGNVKDYPVSEMDALRTIEDKLGR----LTLGLAVVGAILSELPINPS 623
Query: 66 RVL 68
R+L
Sbjct: 624 RLL 626
>At3g25840 protein kinase like protein
Length = 935
Score = 24.6 bits (52), Expect = 9.3
Identities = 16/48 (33%), Positives = 24/48 (49%), Gaps = 1/48 (2%)
Query: 16 LVKGGVQD-LDDGDKSSSRQFNNSLARHINVAAPNFTGIGSMLVQAPD 62
+V+ G+ D DD + S QF L V A + G+ S +V+A D
Sbjct: 589 MVRSGLHDNWDDAEGYYSYQFGELLDGRYEVIATHGKGVFSTVVRAKD 636
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.135 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,461,513
Number of Sequences: 26719
Number of extensions: 45767
Number of successful extensions: 104
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 98
Number of HSP's gapped (non-prelim): 10
length of query: 68
length of database: 11,318,596
effective HSP length: 44
effective length of query: 24
effective length of database: 10,142,960
effective search space: 243431040
effective search space used: 243431040
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146329.10