BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145372.5 - phase: 0
(369 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g06483 pyruvate dehydrogenase like kinase 441 e-124
At1g22340 hypothetical protein 33 0.25
At1g22370 putative UDP-glucose glucosyltransferase 33 0.25
At1g50200 alanine--tRNA ligase, putative 32 0.42
At1g01540 unknown protein 30 1.6
At3g57100 putative protein 30 2.7
At4g36280 unknown protein 29 3.6
At4g02630 putative serine/threonine protein kinase 29 3.6
At4g01330 protein kinase like protein 29 3.6
At5g35020 putative protein 29 4.7
At1g22360 unknown protein 29 4.7
At4g36290 unknown protein 28 6.1
At4g36270 putative protein 28 6.1
At2g23890 hypothetical protein 28 6.1
At3g12590 hypothetical protein 28 8.0
At3g07770 putative heat-shock protein 28 8.0
At1g47600 thioglucosidase, putative 28 8.0
At1g27320 histidine kinase (AHK3) 28 8.0
>At3g06483 pyruvate dehydrogenase like kinase
Length = 272
Score = 441 bits (1135), Expect = e-124
Identities = 215/276 (77%), Positives = 247/276 (88%), Gaps = 5/276 (1%)
Query: 95 IRSFSEIKNMNDEKEFTEVIKAIKVRHNNVVPTMALGVQQLKKDVDPKIINQDFVEIHQF 154
+R+F EIK+ DEK+FT++IKA+KVRHNNVVP MALGV QLKK ++ ++ EIHQF
Sbjct: 1 MRAFPEIKDSGDEKDFTQMIKAVKVRHNNVVPMMALGVNQLKKGMNSGNLD----EIHQF 56
Query: 155 LDRFYMSRIGIRMLIGQHVELHSPNPPPHVVGYIHTRMSPVEVARNAVDDARSICSRQYG 214
LDRFY+SRIGIRMLIGQHVELH+PNPP H VGYIHT+MSP+EVARNA +DARSIC R+YG
Sbjct: 57 LDRFYLSRIGIRMLIGQHVELHNPNPPLHTVGYIHTKMSPMEVARNASEDARSICFREYG 116
Query: 215 SAPDVHIYGDPDFTFPYVPAHLHLMVFELVKNSLRAVEERYMNSDKDPPPIRIIVADGLE 274
SAP+++IYGDP FTFPYVP HLHLM++ELVKNSLRAV+ER+++SD+ PPIRIIVADG+E
Sbjct: 117 SAPEINIYGDPSFTFPYVPTHLHLMMYELVKNSLRAVQERFVDSDRVAPPIRIIVADGIE 176
Query: 275 DVTIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGD-NVTMAGYGYGLPISRL 333
DVTIK+SDEGGGI RSGL +IFTYLYSTARNPL+E DLG+ D VTMAGYGYGLPISRL
Sbjct: 177 DVTIKVSDEGGGIARSGLPRIFTYLYSTARNPLEEDVDLGIADVPVTMAGYGYGLPISRL 236
Query: 334 YARYFGGDLQIISMENYGTDAYLHLSRLGDSQEPLP 369
YARYFGGDLQIISME YGTDAYLHLSRLGDSQEPLP
Sbjct: 237 YARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP 272
>At1g22340 hypothetical protein
Length = 487
Score = 33.1 bits (74), Expect = 0.25
Identities = 22/76 (28%), Positives = 33/76 (42%), Gaps = 13/76 (17%)
Query: 223 GDPDFTFPYVP-----------AHLHLMVFELVKNSLRAVEE--RYMNSDKDPPPIRIIV 269
G P F F +P H + + KN L +E R +N D PP+ IV
Sbjct: 65 GFPSFRFESIPDGLPETDGDRTQHTPTVCMSIEKNCLAPFKEILRRINDKDDVPPVSCIV 124
Query: 270 ADGLEDVTIKISDEGG 285
+DG+ T+ ++E G
Sbjct: 125 SDGVMSFTLDAAEELG 140
>At1g22370 putative UDP-glucose glucosyltransferase
Length = 479
Score = 33.1 bits (74), Expect = 0.25
Identities = 33/120 (27%), Positives = 49/120 (40%), Gaps = 18/120 (15%)
Query: 179 NPPPHVVGYIHTRMSPVEVARNAVDDARSICSRQYGSAPDVHIYGDPDFTFPYVPAHLH- 237
NP V ++ R V + R I SR S + G P F F +P L
Sbjct: 26 NPMLKVAKLLYARGFHVTFVNTNYNHNRLIRSRGPNS-----LDGLPSFRFESIPDGLPE 80
Query: 238 ----------LMVFELVKNSLRAVEE--RYMNSDKDPPPIRIIVADGLEDVTIKISDEGG 285
+ +KN L +E R +N+ KD PP+ IV+DG+ T+ ++E G
Sbjct: 81 ENKDVMQDVPTLCESTMKNCLAPFKELLRRINTTKDVPPVSCIVSDGVMSFTLDAAEELG 140
>At1g50200 alanine--tRNA ligase, putative
Length = 1027
Score = 32.3 bits (72), Expect = 0.42
Identities = 15/32 (46%), Positives = 20/32 (61%), Gaps = 1/32 (3%)
Query: 326 YGLPISRLYARYFGGDLQI-ISMENYGTDAYL 356
YGLP R+YA YFGGD + + +N D +L
Sbjct: 180 YGLPTDRIYATYFGGDEKAGLQPDNEARDIWL 211
>At1g01540 unknown protein
Length = 472
Score = 30.4 bits (67), Expect = 1.6
Identities = 14/51 (27%), Positives = 31/51 (60%), Gaps = 3/51 (5%)
Query: 123 NVVPTMALGVQQLKKDVDPKIINQDFVEIHQFLDRFYMSRI---GIRMLIG 170
N++ MA G+ L + ++PK++++D + LDR + +++ G+ L+G
Sbjct: 256 NIILGMAKGLAYLHEGLEPKVVHRDIKSSNILLDRQWNAKVSDFGLAKLLG 306
>At3g57100 putative protein
Length = 359
Score = 29.6 bits (65), Expect = 2.7
Identities = 14/57 (24%), Positives = 32/57 (55%), Gaps = 5/57 (8%)
Query: 91 SFRDIRSFSEIKNMN----DEKEFTEVIKAIKVRHNNVVPTMALGVQQLKKDVDPKI 143
++RD+R+F+ N N D+ ++ E ++ + H + M + +++ KD+D K+
Sbjct: 145 TYRDLRNFNAFYNNNSGEEDDLDYCEFLRKFQTCHEELA-KMVVKLEKTMKDIDKKL 200
>At4g36280 unknown protein
Length = 626
Score = 29.3 bits (64), Expect = 3.6
Identities = 24/96 (25%), Positives = 39/96 (40%), Gaps = 23/96 (23%)
Query: 282 DEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLPISRLYARYFGGD 341
D+GGG+ +GLRK + YS+ ++ N T+ YG G S + G D
Sbjct: 135 DDGGGMDPAGLRKCMSLGYSSKKS------------NTTIGQYGNGFKTSTM---RLGAD 179
Query: 342 LQII--------SMENYGTDAYLHLSRLGDSQEPLP 369
+ S ++ G +Y L + G +P
Sbjct: 180 AIVFSRSTRGGTSTQSVGILSYTFLRKTGQDDVTVP 215
>At4g02630 putative serine/threonine protein kinase
Length = 492
Score = 29.3 bits (64), Expect = 3.6
Identities = 14/54 (25%), Positives = 31/54 (56%), Gaps = 3/54 (5%)
Query: 123 NVVPTMALGVQQLKKDVDPKIINQDFVEIHQFLDRFYMSRI---GIRMLIGQHV 173
N+V A G+ L + ++PK++++D + LD+ + S++ G+ L+G +
Sbjct: 265 NIVLGTAKGLMYLHEGLEPKVVHRDIKSSNILLDKQWNSKVSDFGLAKLLGSEM 318
>At4g01330 protein kinase like protein
Length = 329
Score = 29.3 bits (64), Expect = 3.6
Identities = 11/41 (26%), Positives = 26/41 (62%)
Query: 123 NVVPTMALGVQQLKKDVDPKIINQDFVEIHQFLDRFYMSRI 163
N++ MA G+ L + ++PK++++D + LDR + +++
Sbjct: 264 NIILCMAKGLAYLHEGLEPKVVHRDIKSSNILLDRQWNAKV 304
>At5g35020 putative protein
Length = 92
Score = 28.9 bits (63), Expect = 4.7
Identities = 12/31 (38%), Positives = 18/31 (57%)
Query: 172 HVELHSPNPPPHVVGYIHTRMSPVEVARNAV 202
+V + PNP +V Y+ R +P+E RN V
Sbjct: 14 YVGIQLPNPISILVNYLPVRQTPIETLRNLV 44
>At1g22360 unknown protein
Length = 481
Score = 28.9 bits (63), Expect = 4.7
Identities = 26/88 (29%), Positives = 40/88 (44%), Gaps = 10/88 (11%)
Query: 200 NAVDDARSICSRQYGSAPDVHIYGDPDFTFPYVPAHLHLMVFELVKNSLRAVEE--RYMN 257
NAVD S ++ S PD D D T + + +K+ L +E R +N
Sbjct: 58 NAVDGLPSF---RFESIPDGLPETDVDVT-----QDIPTLCESTMKHCLAPFKELLRQIN 109
Query: 258 SDKDPPPIRIIVADGLEDVTIKISDEGG 285
+ D PP+ IV+DG T+ ++E G
Sbjct: 110 ARDDVPPVSCIVSDGCMSFTLDAAEELG 137
>At4g36290 unknown protein
Length = 635
Score = 28.5 bits (62), Expect = 6.1
Identities = 19/70 (27%), Positives = 29/70 (41%), Gaps = 15/70 (21%)
Query: 282 DEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLPISRLYARYFGGD 341
D GGG+ +G+RK + YS+ ++ N T+ YG G S + G D
Sbjct: 143 DNGGGMDPNGIRKCMSLGYSSKKS------------NTTIGQYGNGFKTSTM---RLGAD 187
Query: 342 LQIISMENYG 351
+ S G
Sbjct: 188 AMVFSRSTRG 197
>At4g36270 putative protein
Length = 489
Score = 28.5 bits (62), Expect = 6.1
Identities = 27/108 (25%), Positives = 42/108 (38%), Gaps = 26/108 (24%)
Query: 228 TFPYVPAHLHLM--VFELVKNSLRAVEERYMNSDKDPPPIRIIVADGLEDVTIKISDEGG 285
T P PA L+ + ++N V+ +N KD P + D+GG
Sbjct: 80 TNPAAPAIAELIDNAVDEIQNGATFVKIDKINIVKDNSPALVF------------QDDGG 127
Query: 286 GIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLPISRL 333
G+ GLRK + YS+ ++ N T+ YG G S +
Sbjct: 128 GMDPDGLRKCMSLGYSSKKS------------NTTIGQYGNGFKTSTM 163
>At2g23890 hypothetical protein
Length = 546
Score = 28.5 bits (62), Expect = 6.1
Identities = 24/80 (30%), Positives = 40/80 (50%), Gaps = 8/80 (10%)
Query: 218 DVHIYG-DPDFTFPYVPAHLHLMVFELVKNSLRAVEERY----MNSDKDPP-PIRIIVAD 271
++ +YG D D+T + +HL ++++L K + E RY + DP PIR + D
Sbjct: 109 NIQVYGFDYDYTLAHYSSHLQSLIYDLAKKHM-VNEFRYPDVCTQFEYDPTFPIRGLYYD 167
Query: 272 GLEDVTIKISDEGGGIPRSG 291
L+ +K+ D G I G
Sbjct: 168 KLKGCLMKL-DFFGSIEPDG 186
>At3g12590 hypothetical protein
Length = 1211
Score = 28.1 bits (61), Expect = 8.0
Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 4/58 (6%)
Query: 254 RYMNSDKDPPPIRIIVADGLEDVTIKISDEGGGI----PRSGLRKIFTYLYSTARNPL 307
R +S K P P+R VAD L ++ G I P LR + YL ++A L
Sbjct: 29 RSSSSKKPPEPLRRAVADCLSSSPPPVNSHHGAIPSMAPSEALRNLRDYLSASATTDL 86
>At3g07770 putative heat-shock protein
Length = 803
Score = 28.1 bits (61), Expect = 8.0
Identities = 30/122 (24%), Positives = 54/122 (43%), Gaps = 16/122 (13%)
Query: 242 ELVKNSLRAVEE-RYMNS-----DKDPPPIRIIVADGLEDVTIKISDEGGGIPRSGLRKI 295
EL+ N+ A+++ RY++ KD P + I + E+ I ++D G G+ R L
Sbjct: 124 ELISNASDALDKLRYLSVTNPELSKDAPDLDIRIYADKENGIITLTDSGIGMTRQELVDC 183
Query: 296 FTYLYSTA----RNPLDEHADLGVGDNVTMAGYGYGLPISRLYARYFGGDLQIISMENYG 351
+ + L + D G GDN + +G G Y+ + D I+S ++
Sbjct: 184 LGTIAQSGTAKFMKALKDSKDAG-GDNNLIGQFGVG-----FYSAFLVADRVIVSTKSPK 237
Query: 352 TD 353
+D
Sbjct: 238 SD 239
>At1g47600 thioglucosidase, putative
Length = 511
Score = 28.1 bits (61), Expect = 8.0
Identities = 36/132 (27%), Positives = 53/132 (39%), Gaps = 34/132 (25%)
Query: 213 YGSAPDV--HIYGD--PDFTFPYVPAHLHLMVFELVKNSLRAVEERY---MNSDKDPPPI 265
YG P + + GD P+FT P A LVK SL + Y + PPP
Sbjct: 313 YGKYPTIMREMVGDRLPEFT-PEQSA--------LVKGSLDFLGLNYYVTQYATDAPPPT 363
Query: 266 RIIVADGLEDVTIKISDEGGGIPRS-----------GLRKIFTYLYSTARNPLDEHADLG 314
++ + + D + + G+P G R+I Y+ +NPL + G
Sbjct: 364 QL---NAITDARVTLGFYRNGVPIGVVAPSFVYYPPGFRQILNYIKDNYKNPLTYITENG 420
Query: 315 VGD----NVTMA 322
V D NVT+A
Sbjct: 421 VADLDLGNVTLA 432
>At1g27320 histidine kinase (AHK3)
Length = 1036
Score = 28.1 bits (61), Expect = 8.0
Identities = 24/94 (25%), Positives = 35/94 (36%), Gaps = 20/94 (21%)
Query: 259 DKDPPPIRIIVADGLEDVTIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDN 318
+ PP I +IV+ + D G GIP +IFT +G +
Sbjct: 640 EPSPPDINLIVS---------VEDTGVGIPVEAQSRIFT-----------PFMQVGPSIS 679
Query: 319 VTMAGYGYGLPISRLYARYFGGDLQIISMENYGT 352
T G G GL IS+ G++ S G+
Sbjct: 680 RTHGGTGIGLSISKCLVGLMKGEIGFSSTPKVGS 713
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.140 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,613,454
Number of Sequences: 26719
Number of extensions: 383160
Number of successful extensions: 848
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 839
Number of HSP's gapped (non-prelim): 18
length of query: 369
length of database: 11,318,596
effective HSP length: 101
effective length of query: 268
effective length of database: 8,619,977
effective search space: 2310153836
effective search space used: 2310153836
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC145372.5