BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145222.7 - phase: 0
(435 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g14950 unknown protein 583 e-167
At1g05360 unknown protein 538 e-153
At4g00440 unknown protein 29 4.4
At2g14500 hypothetical protein 28 7.6
At1g19750 hypothetical protein 28 7.6
At3g46130 Myb DNA binding protein -like 28 9.9
At3g07720 unknown protein 28 9.9
At2g36380 putative ABC transporter 28 9.9
At2g35060 putative potassium transporter 28 9.9
At1g27840 hypothetical protein 28 9.9
>At4g14950 unknown protein
Length = 416
Score = 583 bits (1503), Expect = e-167
Identities = 281/389 (72%), Positives = 325/389 (83%), Gaps = 7/389 (1%)
Query: 2 ELENLTLTTQPFKTLKYSTLAVIQYIKKTMLYLLAKGGWLMLFSVAVGTLGVVLMTLGCL 61
E+ENLTLTTQP TLK A IQYIK+++ YLLA GGW +L + + G +L+T+
Sbjct: 25 EVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILITTLLVVSGGLLVTVDGP 84
Query: 62 HEKHLEEFLEYFRFGLWWVALGVASSIGLGSGLHTFVLYLGPHIALFTIKAMQCGRVDLK 121
H KH+EE LEY R+GLWW+ALGVASSIGLGSGLHTFVLYLGPHIALFT+KA CGRVDLK
Sbjct: 85 HGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPHIALFTLKATLCGRVDLK 144
Query: 122 SAPYDTIQLNRGPSWLDKNCSQFGPPLFQSEYGTRVPLSSILPQVQMEAVLWGLGTAIGE 181
SAPYDTIQL R PSWLDK+CS+FGPPL S G+RVPL+SILPQVQ+EA+LWG+GTA+GE
Sbjct: 145 SAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILPQVQLEAILWGIGTALGE 204
Query: 182 LPPYFISRAARLSGSRMDAMEELD---SEDKGV----LNQIKCWFFSHTQHLNFFTILVL 234
LPPYFISRAA +SGS +D MEELD +ED G LN++K W +H+QHLNFFT+LVL
Sbjct: 205 LPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKRWLLTHSQHLNFFTVLVL 264
Query: 235 ASVPNPLFDLAGIMCGQFGIPFWNFFLATLIGKAIIKTHIQTVFIISVCNNQLLDWIENE 294
ASVPNPLFDLAGIMCGQFGIPFW FFLATLIGKAIIKTHIQT+FII VCNNQLLDW+ENE
Sbjct: 265 ASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTIFIICVCNNQLLDWMENE 324
Query: 295 FIWVLSHIPGFASVLPKLTANLHAMKDKYLKAPHPVSPNTKGKKWDFSFTSIWNTVVWLM 354
IW+LSH+PG AS+LP LTA LHAMK+KY+ AP PV + K KKWDFSF SIWN +VWLM
Sbjct: 325 LIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPSHIKVKKWDFSFASIWNGIVWLM 384
Query: 355 LMNFFIKIVNSTAQTHLKKQQESEVAALT 383
L+NFF+KIV +TAQ HLKK+QE E+A LT
Sbjct: 385 LLNFFVKIVTATAQRHLKKKQEKEMATLT 413
>At1g05360 unknown protein
Length = 416
Score = 538 bits (1386), Expect = e-153
Identities = 269/389 (69%), Positives = 305/389 (78%), Gaps = 8/389 (2%)
Query: 2 ELENLTLTTQPFKTLKYSTLAVIQYIKKTMLYLLAKGGWLMLFSVAVGTLGVVLMTLGCL 61
+LE LTLT+QPFKTL+ +AV Y+++ YLLA GWL+LF +L+TL
Sbjct: 24 DLEKLTLTSQPFKTLRLFVVAVFLYVRRWSSYLLANVGWLILFCSIFVAFAALLVTLDGP 83
Query: 62 HEKHLEEFLEYFRFGLWWVALGVASSIGLGSGLHTFVLYLGPHIALFTIKAMQCGRVDLK 121
H KH+EE EY RFGLWW+ LGVASSIGLGSGLHTFVLYLGPHIALFTIK MQCGRVDLK
Sbjct: 84 HVKHVEELSEYTRFGLWWIFLGVASSIGLGSGLHTFVLYLGPHIALFTIKVMQCGRVDLK 143
Query: 122 SAPYDTIQLNRGPSWLDKNCSQFGPPLFQSEYGTRVPLSSILPQVQMEAVLWGLGTAIGE 181
SA YDTIQL R PSWLDK C +FG P+F S VPLSSILPQVQ+EA+LWGLGTA+GE
Sbjct: 144 SAIYDTIQLKRSPSWLDKPCHEFGSPVFSSG----VPLSSILPQVQIEAILWGLGTALGE 199
Query: 182 LPPYFISRAARLSGSRMDAMEELDSEDKGVL----NQIKCWFFSHTQHLNFFTILVLASV 237
LPPYFISRAA LSG +M +E +D G + NQIK W SH+Q+LNFFTIL+LASV
Sbjct: 200 LPPYFISRAASLSGGKMKELETCSGDDNGFIAKRVNQIKSWLLSHSQYLNFFTILILASV 259
Query: 238 PNPLFDLAGIMCGQFGIPFWNFFLATLIGKAIIKTHIQTVFIISVCNNQLLDWIENEFIW 297
PNPLFDLAGIMCGQF PFW FFLATLIGKAIIKTHIQTVFII VCNNQLLDW+ENE I+
Sbjct: 260 PNPLFDLAGIMCGQFEKPFWEFFLATLIGKAIIKTHIQTVFIICVCNNQLLDWVENELIY 319
Query: 298 VLSHIPGFASVLPKLTANLHAMKDKYLKAPHPVSPNTKGKKWDFSFTSIWNTVVWLMLMN 357
+LS +PGFAS LP+LTA L MK+KYL A PVS + KKWD SF S+WN VVWLML+N
Sbjct: 320 ILSFVPGFASALPELTAKLRLMKEKYLIASPPVSSDINVKKWDLSFASVWNGVVWLMLLN 379
Query: 358 FFIKIVNSTAQTHLKKQQESEVAALTKKS 386
FF +IV STAQ +LKKQQE E+ ALT KS
Sbjct: 380 FFGQIVTSTAQRYLKKQQEEELDALTNKS 408
>At4g00440 unknown protein
Length = 831
Score = 29.3 bits (64), Expect = 4.4
Identities = 21/74 (28%), Positives = 37/74 (49%), Gaps = 9/74 (12%)
Query: 322 KYLKAPHPVSP-NTKGKKWDFSFTSIWNTVVWLMLMNFFIKIVNSTAQTHLKKQQES--- 377
+ L P +SP N+ G++W+ S T+ + FI +VN +TH + +E+
Sbjct: 426 RILSFPEYLSPLNSPGRRWEKSSTAHKKSA-----SADFINLVNIKKETHASQPEENTDI 480
Query: 378 EVAALTKKSDSDTQ 391
+V L+K+ D Q
Sbjct: 481 QVCNLSKEPDDSIQ 494
>At2g14500 hypothetical protein
Length = 347
Score = 28.5 bits (62), Expect = 7.6
Identities = 15/43 (34%), Positives = 25/43 (57%), Gaps = 2/43 (4%)
Query: 27 IKKTMLYLLAKGGW--LMLFSVAVGTLGVVLMTLGCLHEKHLE 67
+ + L+ + G W +F++ V T G VLM L L+EK++E
Sbjct: 239 VTRNYLFNVHNGPWGFRSIFNLVVTTSGEVLMVLNNLYEKNIE 281
>At1g19750 hypothetical protein
Length = 254
Score = 28.5 bits (62), Expect = 7.6
Identities = 19/68 (27%), Positives = 30/68 (43%), Gaps = 1/68 (1%)
Query: 185 YFISRAARLSGSRMDAMEELDSEDKGVLNQIKCWFFSHTQHLNFFTILVLASVPNPLFDL 244
Y +S AA S + D D E G++ + KC F QH N + +++ P+ D
Sbjct: 58 YLLSGAADGSAAVFDVKRATDYEASGLIAKHKCIFTVRKQHENGHKYAISSAIWYPI-DT 116
Query: 245 AGIMCGQF 252
+ G F
Sbjct: 117 GMFITGSF 124
>At3g46130 Myb DNA binding protein -like
Length = 256
Score = 28.1 bits (61), Expect = 9.9
Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 3/58 (5%)
Query: 292 ENEFIWVLSHIPGFASVLPKLTANLHAMKDKYLKAPHPVS-PNTKGKKWDFSFTSIWN 348
ENE ++ L I + + + +KD Y + H +S PN W+ S SIWN
Sbjct: 170 ENEDVYSLDDI--WREIDHSAVNIIKPVKDIYSEQSHCLSYPNLASPSWESSLDSIWN 225
>At3g07720 unknown protein
Length = 329
Score = 28.1 bits (61), Expect = 9.9
Identities = 19/70 (27%), Positives = 31/70 (44%), Gaps = 1/70 (1%)
Query: 149 FQSEYGTRVPLSSILPQVQMEAVLWGLGTAIGELPPYFISRAARLSGSRMDAMEELDSED 208
F E+ RVP+ + L +E W + A G+ PP + A G + DS
Sbjct: 38 FGGEFQPRVPVDNQLYVFDLETQTWSIQEASGDAPPPRVGVAMAAVGPIIYFFGGRDSTH 97
Query: 209 KGVLNQIKCW 218
+ LN++ C+
Sbjct: 98 Q-ELNELYCF 106
>At2g36380 putative ABC transporter
Length = 1450
Score = 28.1 bits (61), Expect = 9.9
Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 7/52 (13%)
Query: 218 WFFSHTQ----HLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWNFFLATLI 265
WF+ + + + ++++A PN + +AGI C F + FWN F LI
Sbjct: 1310 WFYYYMLTCFVYFTLYGMMLVALTPN--YQIAGI-CLSFFLSFWNLFSGFLI 1358
>At2g35060 putative potassium transporter
Length = 792
Score = 28.1 bits (61), Expect = 9.9
Identities = 27/80 (33%), Positives = 38/80 (46%), Gaps = 9/80 (11%)
Query: 31 MLYLLAKGGWLMLFSVAVGTLGVVLMTLGCLHEKHLEEFLEYFRFGLWWVALGVASSIGL 90
ML+ + +GGW+ L A L + + G L EF + R + W+ LG+ S+GL
Sbjct: 500 MLFKIDQGGWVPLVIAAAFLLIMWVWHYGTLKR---YEFEMHCRVSMAWI-LGLGPSLGL 555
Query: 91 ----GSGLHTFVLYLG-PHI 105
G GL L G PHI
Sbjct: 556 VRVPGVGLVYTELASGVPHI 575
>At1g27840 hypothetical protein
Length = 254
Score = 28.1 bits (61), Expect = 9.9
Identities = 19/68 (27%), Positives = 30/68 (43%), Gaps = 1/68 (1%)
Query: 185 YFISRAARLSGSRMDAMEELDSEDKGVLNQIKCWFFSHTQHLNFFTILVLASVPNPLFDL 244
Y +S AA S + D D E G++ + KC F QH N + +++ P+ D
Sbjct: 58 YLLSGAADGSAAVFDVQRATDYEASGLIAKHKCIFTVDKQHENGHKYAISSAIWYPI-DT 116
Query: 245 AGIMCGQF 252
+ G F
Sbjct: 117 GLFITGSF 124
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.331 0.143 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,482,399
Number of Sequences: 26719
Number of extensions: 393650
Number of successful extensions: 1111
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1105
Number of HSP's gapped (non-prelim): 10
length of query: 435
length of database: 11,318,596
effective HSP length: 102
effective length of query: 333
effective length of database: 8,593,258
effective search space: 2861554914
effective search space used: 2861554914
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC145222.7