BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145219.5 + phase: 0
(130 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g40080 unknown protein 113 3e-26
At2g29950 hypothetical protein 94 2e-20
At2g06255 unknown protein 84 2e-17
At1g72630 unknown protein 77 3e-15
At3g63400 cyclophylin (ROC22) 36 0.005
At4g37250 receptor kinase like protein 31 0.15
At5g53210 putative bHLH transcription factor (AtbHLH089) 30 0.26
At1g11420 hypothetical protein 30 0.45
At4g08480 putative mitogen-activated protein kinase 29 0.59
At5g28670 putative protein 29 0.76
At4g16050 hypothetical protein 29 0.76
At1g36730 putative protein 29 0.76
At5g10250 non-phototropic hypocotyl 3-like protein 28 1.00
At4g24680 unknown protein 28 1.3
At3g51650 putative protein 28 1.3
At5g16690 unknown protein 28 1.7
At4g25580 unknown protein 28 1.7
At5g49400 putative protein 27 2.2
At3g31500 hypothetical protein 27 2.2
At4g09940 AIG1-like protein 27 2.9
>At2g40080 unknown protein
Length = 111
Score = 113 bits (282), Expect = 3e-26
Identities = 55/81 (67%), Positives = 68/81 (83%), Gaps = 1/81 (1%)
Query: 40 EDAEDG-DPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNG 98
E+AE G DP W+ L+++FRQVQSVLDRNR++IQQVN+N QSRM DNM KNV LIQELNG
Sbjct: 15 EEAEQGEDPAMWENLDRNFRQVQSVLDRNRSLIQQVNDNHQSRMADNMSKNVALIQELNG 74
Query: 99 NISKVASLYSDLNSDFTNICH 119
NISKV ++YSDLN+ F++ H
Sbjct: 75 NISKVVNMYSDLNTSFSSGFH 95
>At2g29950 hypothetical protein
Length = 125
Score = 94.0 bits (232), Expect = 2e-20
Identities = 52/116 (44%), Positives = 72/116 (61%), Gaps = 4/116 (3%)
Query: 11 MEDTNHRSKKNQRRRRPSATPTNNDYSDVE-DAEDGDPEAWQTLNKSFRQVQSVLDRNRA 69
ME + +RS R S ND DV A D E W TL+ F++ Q LD+NR
Sbjct: 1 MEASRNRSLVGNNR---SPEMNENDGEDVAASAAVEDVEVWDTLSNGFKRAQLYLDQNRD 57
Query: 70 IIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNICHQQQRSS 125
+IQ+VNEN SR+PDN+ +NVGLI E+NGNIS+V +YSDL+ +F Q++R++
Sbjct: 58 LIQRVNENHMSRIPDNVSRNVGLINEINGNISQVMEIYSDLSLNFAKKFDQRRRTT 113
>At2g06255 unknown protein
Length = 109
Score = 84.0 bits (206), Expect = 2e-17
Identities = 37/85 (43%), Positives = 58/85 (67%)
Query: 46 DPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVAS 105
D + QT KSF QVQ++LD NR +I ++N+N +S++PDN+ +NVGLI+ELN N+ +VA
Sbjct: 17 DGKILQTFEKSFVQVQNILDHNRLLINEINQNHESKIPDNLGRNVGLIRELNNNVRRVAH 76
Query: 106 LYSDLNSDFTNICHQQQRSSHNRGK 130
LY DL+++F+ + G+
Sbjct: 77 LYVDLSNNFSKSMEASSEGDSSEGR 101
>At1g72630 unknown protein
Length = 119
Score = 76.6 bits (187), Expect = 3e-15
Identities = 37/79 (46%), Positives = 52/79 (64%)
Query: 36 YSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQE 95
YS + D + Q KSF QVQ +LD+NR +I ++N+N +S+ D++ +NVGLI+E
Sbjct: 10 YSGFGERYQMDGKLLQNFQKSFVQVQDILDQNRLLINEINQNHESKQADHLGRNVGLIRE 69
Query: 96 LNGNISKVASLYSDLNSDF 114
LN NI VASLY DL+ F
Sbjct: 70 LNNNIRTVASLYGDLSHSF 88
>At3g63400 cyclophylin (ROC22)
Length = 567
Score = 36.2 bits (82), Expect = 0.005
Identities = 29/109 (26%), Positives = 52/109 (47%), Gaps = 20/109 (18%)
Query: 17 RSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQ------------SVL 64
+ + +++ RP P+ N SD E + D E + +K+ + V+ SV
Sbjct: 282 KGRSGKKKARPDRKPSTNSSSDTESSSSSDDE--KVGHKAIKSVKVDNADQHANLDDSVK 339
Query: 65 DRNRAIIQQVNENQQSRMPD-NMVKNVGLIQELNGNISKVASLYSDLNS 112
R+R+ I++ N+N +S+ P + V+ +G NGN S S DL +
Sbjct: 340 SRSRSPIRRRNQNSRSKSPSRSPVRVLG-----NGNRSPSRSPVRDLGN 383
>At4g37250 receptor kinase like protein
Length = 768
Score = 31.2 bits (69), Expect = 0.15
Identities = 16/45 (35%), Positives = 24/45 (52%)
Query: 1 MSTLYSPSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDG 45
+ST S SS+ E++ K + R+ P TP+ + D ED E G
Sbjct: 377 LSTFSSSSSSPEESRRFRKWSCLRKDPETTPSEEEDEDDEDEESG 421
>At5g53210 putative bHLH transcription factor (AtbHLH089)
Length = 294
Score = 30.4 bits (67), Expect = 0.26
Identities = 29/112 (25%), Positives = 52/112 (45%), Gaps = 11/112 (9%)
Query: 6 SPSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQS--V 63
S ++D ++ + +R+++ T D E+ EDGD EA + + +Q S
Sbjct: 51 SSKELVKDQDYENSSPKRKKQRLETRKEED----EEEEDGDGEAEEDNKQDGQQKMSHVT 106
Query: 64 LDRNRAIIQQVNEN---QQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNS 112
++RNR +Q+NE+ +S MP VK + G + ++ L L S
Sbjct: 107 VERNRR--KQMNEHLTVLRSLMPCFYVKRGDQASIIGGVVEYISELQQVLQS 156
>At1g11420 hypothetical protein
Length = 604
Score = 29.6 bits (65), Expect = 0.45
Identities = 17/58 (29%), Positives = 28/58 (47%), Gaps = 2/58 (3%)
Query: 35 DYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGL 92
++SD+ + + W K RQ ++ LD NR + Q E + M D++ K GL
Sbjct: 259 NHSDIRPPMEWEDGVWHKRTKPKRQKETSLDGNRNV--QTKEPPGNEMADDVKKESGL 314
>At4g08480 putative mitogen-activated protein kinase
Length = 773
Score = 29.3 bits (64), Expect = 0.59
Identities = 22/79 (27%), Positives = 36/79 (44%), Gaps = 3/79 (3%)
Query: 7 PSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDR 66
P S+ + H + ++ RRPS++ ++ + D E+AED E +T F Q++ D
Sbjct: 411 PGSSWDILTHFAPDSEIVRRPSSSSSSENGCDEEEAEDDKVEKEET-GDMFIQLEDTTD- 468
Query: 67 NRAIIQQVNENQQSRMPDN 85
A NE S N
Sbjct: 469 -EACSFTTNEGDSSSTVSN 486
>At5g28670 putative protein
Length = 647
Score = 28.9 bits (63), Expect = 0.76
Identities = 25/118 (21%), Positives = 49/118 (41%), Gaps = 12/118 (10%)
Query: 12 EDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNK---SFRQVQSVLDRNR 68
ED ++ + + P +DY D + +D AW LNK SF + R
Sbjct: 400 EDVTEQNNVGETNKDPQERNQVHDYYDCSEHDDTQFMAWLELNKPLQSFEVDEEHETSPR 459
Query: 69 AIIQQVNENQQSRMP--DNMVKNV-------GLIQELNGNISKVASLYSDLNSDFTNI 117
I+ ++ + +P DN ++++ QE + +S+ ++ +N D +I
Sbjct: 460 YILTRIQISDDILIPEGDNPIESIIRAVYGTTFPQEKSPTVSQGRAILCPINEDVNSI 517
>At4g16050 hypothetical protein
Length = 900
Score = 28.9 bits (63), Expect = 0.76
Identities = 16/71 (22%), Positives = 35/71 (48%), Gaps = 1/71 (1%)
Query: 11 MEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLN-KSFRQVQSVLDRNRA 69
++D ++K + +RR A + ++ ED E D E ++ + + ++ D N
Sbjct: 721 LDDEMKKAKHSTNKRRKRAREDDESAAETEDDESADTEDDESADTEDDESAETEDDDNMT 780
Query: 70 IIQQVNENQQS 80
I Q++N ++S
Sbjct: 781 IAQRINSRKKS 791
>At1g36730 putative protein
Length = 439
Score = 28.9 bits (63), Expect = 0.76
Identities = 31/120 (25%), Positives = 48/120 (39%), Gaps = 25/120 (20%)
Query: 20 KNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQT------LNKSFRQVQSVLDRNRAII-- 71
K + P + ND +D E ED D WQT K ++ S + ++
Sbjct: 202 KKKDHSPPRSLSDENDQADSE--EDDDDVQWQTDTSREAAEKRMKEQLSAVTAEMVMLST 259
Query: 72 --------------QQVNENQQSRMPDNM-VKNVGLIQELNGNISKVASLYSDLNSDFTN 116
+QV+EN S++P+N K V I+EL + S L + L S+ N
Sbjct: 260 VEEKKPVAEVKKAPEQVHENGNSKIPENAHEKLVNEIKELLSSGSSPTQLKTALASNSAN 319
>At5g10250 non-phototropic hypocotyl 3-like protein
Length = 607
Score = 28.5 bits (62), Expect = 1.00
Identities = 17/64 (26%), Positives = 30/64 (46%), Gaps = 6/64 (9%)
Query: 72 QQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNICHQ------QQRSS 125
++ EN R M ++ +I+ L + V S+L SD+ + + +Q+SS
Sbjct: 502 EKEEENSGGREDKRMSRDNEIIKTLKEELENVKKKMSELQSDYNELQQEYERLSSKQKSS 561
Query: 126 HNRG 129
HN G
Sbjct: 562 HNWG 565
>At4g24680 unknown protein
Length = 1480
Score = 28.1 bits (61), Expect = 1.3
Identities = 30/140 (21%), Positives = 59/140 (41%), Gaps = 13/140 (9%)
Query: 2 STLYSPSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQ 61
ST+ + S+ + N+R+ K + + +AT + ++ + GD E+ +T +++
Sbjct: 909 STVSAESTFPKRKNNRNGKKKHKAEETATMNTTRVAVGKETKSGD-ESIETARARAAEIE 967
Query: 62 --SVL----------DRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSD 109
SV D + I NE Q+R +N + N ++K A +S
Sbjct: 968 LGSVSVPSLDIKVSGDSSEQISSFTNEESQNRAKNNWKSQHVRRTQRNSLVNKPAEKFSG 1027
Query: 110 LNSDFTNICHQQQRSSHNRG 129
N+ H QQ++ + G
Sbjct: 1028 NNAVIWAPVHPQQKADVSTG 1047
>At3g51650 putative protein
Length = 826
Score = 28.1 bits (61), Expect = 1.3
Identities = 16/55 (29%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Query: 5 YSPSSAMEDTNHRSKKNQRRRRP---SATPTNNDYSDVEDAEDGDPEAWQTLNKS 56
+S ++ +DT KK Q RR+ +++ +N+D +V+ + E + LNKS
Sbjct: 306 FSVAAKEKDTKEAEKKRQERRKERDRASSKSNSDGEEVDKRTRKETEQKRGLNKS 360
>At5g16690 unknown protein
Length = 734
Score = 27.7 bits (60), Expect = 1.7
Identities = 20/72 (27%), Positives = 33/72 (45%), Gaps = 6/72 (8%)
Query: 15 NHRSKKN----QRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAI 70
NH K +RR S +P S+VE E+ D + + TL F+ ++V +
Sbjct: 42 NHNGKLTGIVKSKRRIESPSPKIAKRSEVESVEEEDGQFFSTLR--FKVFETVWSKIEKT 99
Query: 71 IQQVNENQQSRM 82
I+ V N S++
Sbjct: 100 IEDVLRNSNSKV 111
>At4g25580 unknown protein
Length = 626
Score = 27.7 bits (60), Expect = 1.7
Identities = 23/83 (27%), Positives = 36/83 (42%), Gaps = 8/83 (9%)
Query: 19 KKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQQVN--- 75
+K +RR P+ TP N S + ED +KS Q + +DR + + Q
Sbjct: 147 EKEERRGAPTLTPHNTPVSLLSATEDVTRTFVPDEHKSRNQSKVNIDRPKGLDQDPAAPG 206
Query: 76 -----ENQQSRMPDNMVKNVGLI 93
N QS++ D + K+ G I
Sbjct: 207 SHGGLSNYQSKVTDPIGKSTGEI 229
>At5g49400 putative protein
Length = 280
Score = 27.3 bits (59), Expect = 2.2
Identities = 20/68 (29%), Positives = 30/68 (43%), Gaps = 11/68 (16%)
Query: 2 STLYSPSSAMEDTNHRSKK-----------NQRRRRPSATPTNNDYSDVEDAEDGDPEAW 50
S+ YS SS ED R +K +RRRR S++ + + S+ D D +
Sbjct: 207 SSEYSSSSDSEDERRRRRKAKKSKKKQKQRKERRRRYSSSSSESSESESASDSDSDEDRS 266
Query: 51 QTLNKSFR 58
+ KS R
Sbjct: 267 RRKKKSKR 274
>At3g31500 hypothetical protein
Length = 591
Score = 27.3 bits (59), Expect = 2.2
Identities = 15/56 (26%), Positives = 27/56 (47%)
Query: 68 RAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNICHQQQR 123
+ ++QQV E+Q+ D VK + +LNG + ++S L + + I R
Sbjct: 165 KLMMQQVLEDQKKNAADINVKVDSMYNDLNGKFATLSSHVKTLENQVSQIVSASMR 220
>At4g09940 AIG1-like protein
Length = 394
Score = 26.9 bits (58), Expect = 2.9
Identities = 11/59 (18%), Positives = 35/59 (58%), Gaps = 6/59 (10%)
Query: 58 RQVQSVLDRNRAIIQQVNENQ------QSRMPDNMVKNVGLIQELNGNISKVASLYSDL 110
++ + V R+ +++++N+ Q +SR + + ++ G+++ LN ++++ + +DL
Sbjct: 326 KRAKEVEKRSSDVVKELNDEQAKRLESESRAKEAVKQSNGVVENLNKELARIKQMATDL 384
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.309 0.123 0.340
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,995,238
Number of Sequences: 26719
Number of extensions: 115923
Number of successful extensions: 470
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 26
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 436
Number of HSP's gapped (non-prelim): 53
length of query: 130
length of database: 11,318,596
effective HSP length: 88
effective length of query: 42
effective length of database: 8,967,324
effective search space: 376627608
effective search space used: 376627608
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC145219.5