BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144929.2 + phase: 0
(120 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g32110 non-LTR reverse transcriptase, putative 43 3e-05
At1g32390 hypothetical protein 37 0.002
At2g23880 putative non-LTR retroelement reverse transcriptase 37 0.002
At2g16680 putative non-LTR retroelement reverse transcriptase 37 0.002
At2g41580 putative non-LTR retroelement reverse transcriptase 36 0.003
At3g25270 hypothetical protein 35 0.008
At2g17910 putative non-LTR retroelement reverse transcriptase 35 0.008
At1g31030 F17F8.5 35 0.008
At2g45230 putative non-LTR retroelement reverse transcriptase 34 0.010
At1g19030 hypothetical protein 34 0.010
At3g28160 non-LTR reverse transcriptase, putative 34 0.013
At1g23990 reverse transcriptase, putative 33 0.023
At3g42890 putative protein 33 0.030
At4g11710 putative protein 32 0.039
At2g05980 putative non-LTR retroelement reverse transcriptase 32 0.039
At3g17200 hypothetical protein 32 0.066
At1g47910 reverse transcriptase, putative 32 0.066
At1g47850 hypothetical protein 32 0.066
At1g21330 hypothetical protein 32 0.066
At4g09710 RNA-directed DNA polymerase -like protein 31 0.086
>At3g32110 non-LTR reverse transcriptase, putative
Length = 1911
Score = 42.7 bits (99), Expect = 3e-05
Identities = 24/86 (27%), Positives = 43/86 (49%), Gaps = 4/86 (4%)
Query: 1 MFTNEKPAIADVTTNF--IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCV 58
M TN+ D+++ + +W Q P +V + W L+ N+ +M ++ R L D+ +C
Sbjct: 1564 MLTNDDSPRQDMSSLYGRVWKVQAPERVRVFLW-LVVNQAIMTNSERKRRHLC-DSDVCQ 1621
Query: 59 TGCGGLEIAQHLFLSCPCFNSQWDLV 84
GG+E H+ CP + WD +
Sbjct: 1622 VCRGGIESILHVLRDCPAMSGIWDRI 1647
>At1g32390 hypothetical protein
Length = 192
Score = 37.0 bits (84), Expect = 0.002
Identities = 22/67 (32%), Positives = 28/67 (40%), Gaps = 6/67 (8%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMV--RGTLSHDNQLCVTGCGGLEIAQHLFLSC 74
+W K S AW +RNRL M D +M GT + CV +E HLF C
Sbjct: 69 VWFAHATPKFSFCAWLAVRNRLSMVDRMMTWNNGTPT----TCVFCSSPMETRDHLFFQC 124
Query: 75 PCFNSQW 81
+ W
Sbjct: 125 HYSSEIW 131
>At2g23880 putative non-LTR retroelement reverse transcriptase
Length = 1216
Score = 36.6 bits (83), Expect = 0.002
Identities = 23/66 (34%), Positives = 30/66 (44%), Gaps = 7/66 (10%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMV--RGTLSHDNQLCVTGCGGLEIAQHLFLSC 74
+W K S AW IRNRL D +M GT + CV +E HLF C
Sbjct: 782 VWFAHATPKFSFCAWLAIRNRLSTGDRMMTWNNGTPT----TCVFCSSPMETRDHLFFQC 837
Query: 75 PCFNSQ 80
C++S+
Sbjct: 838 -CYSSE 842
>At2g16680 putative non-LTR retroelement reverse transcriptase
Length = 1319
Score = 36.6 bits (83), Expect = 0.002
Identities = 17/67 (25%), Positives = 29/67 (42%), Gaps = 2/67 (2%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
+W + K+ + W+ ++ L ++D L RG + D C+ +E HL CP
Sbjct: 998 VWSLETVPKIKVFMWKALKGALAVEDRLRSRGIRTADG--CLFCKEEIETINHLLFQCPF 1055
Query: 77 FNSQWDL 83
W L
Sbjct: 1056 ARQVWAL 1062
>At2g41580 putative non-LTR retroelement reverse transcriptase
Length = 1094
Score = 36.2 bits (82), Expect = 0.003
Identities = 17/67 (25%), Positives = 28/67 (41%), Gaps = 2/67 (2%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
IW+ K+ + W++++ + ++D L RG L D C E H+ CP
Sbjct: 775 IWNLNSAPKIKVFLWKVLKGAVAVEDRLRTRGVLIEDG--CSMCPEKNETLNHILFQCPL 832
Query: 77 FNSQWDL 83
W L
Sbjct: 833 ARQVWAL 839
>At3g25270 hypothetical protein
Length = 343
Score = 34.7 bits (78), Expect = 0.008
Identities = 20/65 (30%), Positives = 26/65 (39%), Gaps = 2/65 (3%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
IW + K+ W+L+ L DNL R +H C C E +QHLF C
Sbjct: 18 IWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQ--CHRCCQEDETSQHLFFDCFY 75
Query: 77 FNSQW 81
W
Sbjct: 76 AQQVW 80
>At2g17910 putative non-LTR retroelement reverse transcriptase
Length = 1344
Score = 34.7 bits (78), Expect = 0.008
Identities = 17/65 (26%), Positives = 26/65 (39%), Gaps = 2/65 (3%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
IW+ K+ I W+ + + ++D L RG S D C+ E H+ CP
Sbjct: 1024 IWNLHTAPKIRIFLWKALHGAIPVEDRLRTRGIRSDDG--CLMCDTENETINHILFECPL 1081
Query: 77 FNSQW 81
W
Sbjct: 1082 ARQVW 1086
>At1g31030 F17F8.5
Length = 872
Score = 34.7 bits (78), Expect = 0.008
Identities = 16/65 (24%), Positives = 28/65 (42%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
+W + K ++ W I NRL D ++ + + CV + +HLF SC
Sbjct: 707 VWFRHATPKYALCTWLAIHNRLPTGDRMLKWNSSGSVSGNCVLCTNNSKTLEHLFFSCSY 766
Query: 77 FNSQW 81
++ W
Sbjct: 767 ASTVW 771
>At2g45230 putative non-LTR retroelement reverse transcriptase
Length = 1374
Score = 34.3 bits (77), Expect = 0.010
Identities = 20/65 (30%), Positives = 24/65 (36%), Gaps = 2/65 (3%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
IW VP K+ WR + N L + NL R + CV E HL CP
Sbjct: 1054 IWKLDVPPKIHHFLWRCVNNCLSVASNLAYRHLAREKS--CVRCPSHGETVNHLLFKCPF 1111
Query: 77 FNSQW 81
W
Sbjct: 1112 ARLTW 1116
>At1g19030 hypothetical protein
Length = 398
Score = 34.3 bits (77), Expect = 0.010
Identities = 21/67 (31%), Positives = 28/67 (41%), Gaps = 2/67 (2%)
Query: 15 NFIWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSC 74
N IW K K S++ W + +NR+ D L+ N C+ LE HLF C
Sbjct: 287 NGIWFKHSTPKYSLITWLVQKNRVATGDKLIKWNPQVTPN--CILCQHTLETWDHLFFMC 344
Query: 75 PCFNSQW 81
P W
Sbjct: 345 PYSFQVW 351
>At3g28160 non-LTR reverse transcriptase, putative
Length = 630
Score = 33.9 bits (76), Expect = 0.013
Identities = 21/65 (32%), Positives = 24/65 (36%), Gaps = 2/65 (3%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
+W K S + W NRL D L S CV LE HLF SCP
Sbjct: 283 VWFPSSTPKYSFVTWLAFHNRLATGDRLYKWN--SEARATCVFCDEELETRDHLFFSCPY 340
Query: 77 FNSQW 81
+ W
Sbjct: 341 SSQIW 345
>At1g23990 reverse transcriptase, putative
Length = 657
Score = 33.1 bits (74), Expect = 0.023
Identities = 20/67 (29%), Positives = 30/67 (43%), Gaps = 4/67 (5%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
IW K S AW ++NRL D ++ + CV +E HLF SC C
Sbjct: 494 IWFAHATPKFSFCAWLAVQNRLSTGDKMLQWN--RRLSPTCVLCNNNIETRNHLFFSC-C 550
Query: 77 FNSQ-WD 82
+ ++ W+
Sbjct: 551 YTAEIWE 557
>At3g42890 putative protein
Length = 534
Score = 32.7 bits (73), Expect = 0.030
Identities = 18/65 (27%), Positives = 28/65 (42%), Gaps = 2/65 (3%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
+W K S + W RNRL + L+ + N C +E +H+F SCP
Sbjct: 108 VWFPNSTPKYSFITWIAFRNRLATGEKLVKWN--NDANGGCSFCDEAMETREHIFFSCPY 165
Query: 77 FNSQW 81
++ W
Sbjct: 166 SSAVW 170
>At4g11710 putative protein
Length = 473
Score = 32.3 bits (72), Expect = 0.039
Identities = 20/66 (30%), Positives = 26/66 (39%), Gaps = 2/66 (3%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
+W Q K + W + NRL D M + D C+ LE HLF SCP
Sbjct: 309 VWFAQAIPKHAFCMWLAVHNRLSTGDR-MTLWNMGVD-ATCILCNKALESRDHLFFSCPF 366
Query: 77 FNSQWD 82
W+
Sbjct: 367 ATEIWE 372
>At2g05980 putative non-LTR retroelement reverse transcriptase
Length = 1352
Score = 32.3 bits (72), Expect = 0.039
Identities = 19/65 (29%), Positives = 26/65 (39%), Gaps = 2/65 (3%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
+W K S + W NRL D + + + + CV LE HLF SCP
Sbjct: 1207 VWFSASTPKYSFVTWLAFHNRLTTSDKICKWNSGARYD--CVFCGEELETRDHLFFSCPY 1264
Query: 77 FNSQW 81
+ W
Sbjct: 1265 SSHVW 1269
>At3g17200 hypothetical protein
Length = 310
Score = 31.6 bits (70), Expect = 0.066
Identities = 16/66 (24%), Positives = 26/66 (39%), Gaps = 2/66 (3%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
+W K S + W + +NR+ D +++ H N C +E HLF C
Sbjct: 144 VWFSHSTPKYSFITWLVSKNRMATGDRMVLWN--QHVNTSCSLCDEPMETRDHLFFVCTY 201
Query: 77 FNSQWD 82
W+
Sbjct: 202 SRKVWE 207
>At1g47910 reverse transcriptase, putative
Length = 1142
Score = 31.6 bits (70), Expect = 0.066
Identities = 21/75 (28%), Positives = 33/75 (44%), Gaps = 4/75 (5%)
Query: 11 DVTT--NFIWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQ 68
D+TT +IW Q P K+ W+++ + + +NL RG L ++ CV+ E
Sbjct: 820 DLTTLKAYIWKVQCPPKLRHFLWQILSGCVPVSENLRKRGILC--DKGCVSCGASEESIN 877
Query: 69 HLFLSCPCFNSQWDL 83
H C W L
Sbjct: 878 HTLFQCHPARQIWAL 892
>At1g47850 hypothetical protein
Length = 799
Score = 31.6 bits (70), Expect = 0.066
Identities = 22/67 (32%), Positives = 30/67 (43%), Gaps = 4/67 (5%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQ-HLFLSCP 75
+W K S L W I +RL D + + N CV CG +E ++ HLF SC
Sbjct: 634 VWFSYNTPKYSFLLWLAIHDRLSTGDRIKKWNSGQQVN--CVL-CGNVEESRNHLFFSCH 690
Query: 76 CFNSQWD 82
+ WD
Sbjct: 691 YTTAIWD 697
>At1g21330 hypothetical protein
Length = 211
Score = 31.6 bits (70), Expect = 0.066
Identities = 21/69 (30%), Positives = 28/69 (40%), Gaps = 7/69 (10%)
Query: 18 WHKQVPL-----KVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFL 72
WH+ V L K S + W +RNRL D ++ N CV +E HLF
Sbjct: 44 WHQGVWLNHHKPKFSFMTWLAVRNRLSTGDRMLQWN--MGVNSCCVFCHAPVETRNHLFF 101
Query: 73 SCPCFNSQW 81
+C W
Sbjct: 102 ACQYSADVW 110
>At4g09710 RNA-directed DNA polymerase -like protein
Length = 1274
Score = 31.2 bits (69), Expect = 0.086
Identities = 18/67 (26%), Positives = 28/67 (40%), Gaps = 3/67 (4%)
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
IW KV W+ ++ L + + L R + ++ CG E + HL L CP
Sbjct: 974 IWKIHTSPKVKHFLWKAMKGALPVGEALSRRNI---EAEVTCKRCGQTESSLHLMLLCPY 1030
Query: 77 FNSQWDL 83
W+L
Sbjct: 1031 AKKVWEL 1037
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.328 0.139 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,820,708
Number of Sequences: 26719
Number of extensions: 102576
Number of successful extensions: 311
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 33
Number of HSP's that attempted gapping in prelim test: 276
Number of HSP's gapped (non-prelim): 54
length of query: 120
length of database: 11,318,596
effective HSP length: 96
effective length of query: 24
effective length of database: 8,753,572
effective search space: 210085728
effective search space used: 210085728
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144929.2