BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144807.2 + phase: 0
(239 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g33250 unknown protein 318 2e-87
At2g41250 unknown protein (At2g41250) 37 0.007
At5g14950 alpha-mannosidase -like protein 35 0.046
At5g36790 p-nitrophenylphosphatase-like protein 33 0.18
At5g36700 N-glyceraldehyde-2-phosphotransferase-like 33 0.18
At5g13540 putative protein 30 1.1
At5g47760 4-nitrophenylphosphatase-like protein 29 1.9
At5g44730 Dreg-2 like protein 28 3.3
At4g11570 unknown protein 28 4.3
At3g58830 putative protein 28 4.3
At1g06470 integral membrane protein, putative 28 4.3
At3g18810 protein kinase, putative 28 5.7
At3g59530 unknown protein 27 7.4
At2g39360 putative protein kinase 27 7.4
At5g12210 Rab geranylgeranyltransferase, beta subunit 27 9.7
>At2g33250 unknown protein
Length = 529
Score = 318 bits (814), Expect = 2e-87
Identities = 148/216 (68%), Positives = 183/216 (84%)
Query: 24 KTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGDDEYLRVKASNPVGIDILKLIDHWSPLQQ 83
KTRLRGVVFDMDGTLTVPVIDF +MY+AVLG+D Y R+KA +P GIDIL I+ WSP +Q
Sbjct: 314 KTRLRGVVFDMDGTLTVPVIDFAAMYRAVLGEDAYKRIKAESPSGIDILHHIESWSPDKQ 373
Query: 84 RQAYDTIAHFEKQALDHLQIMPGAADLCNVLDSKKIRRGLITRNMKSAVDLFHQRFGITF 143
++AY+ IA +EKQ +D LQIMPG A+LC LDSKKI+RGLITRN++ A+D+FHQRF + F
Sbjct: 374 QKAYEIIADYEKQGIDKLQIMPGTAELCGFLDSKKIKRGLITRNVQKAIDIFHQRFEVIF 433
Query: 144 SPALSREFRPYKPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQTG 203
SPAL REFRPYKP+P PLLHICS W++QPNEV+MVGDSLKDDIACG RAGA TCLLD+TG
Sbjct: 434 SPALGREFRPYKPNPDPLLHICSTWDIQPNEVMMVGDSLKDDIACGKRAGAFTCLLDETG 493
Query: 204 RYDSPEYANVDFKPDFKVTSLAEVYSILETNFELSP 239
RY +++ +PDFKV SL+++ ++LETNF+L+P
Sbjct: 494 RYGPDDFSVSGLQPDFKVDSLSKIQNLLETNFDLNP 529
>At2g41250 unknown protein (At2g41250)
Length = 290
Score = 37.4 bits (85), Expect = 0.007
Identities = 19/53 (35%), Positives = 26/53 (48%)
Query: 146 ALSREFRPYKPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCL 198
A+S E KP+P L C L EV P + + VGD ++D+ AG L
Sbjct: 219 AVSAEVEAEKPNPTIFLKACELLEVNPEDAVHVGDDRRNDVWGARDAGCDAWL 271
>At5g14950 alpha-mannosidase -like protein
Length = 1173
Score = 34.7 bits (78), Expect = 0.046
Identities = 32/117 (27%), Positives = 50/117 (42%), Gaps = 21/117 (17%)
Query: 144 SPALSREFRPYKPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGG------RAGAHTC 197
+P+ + + P P P + CS + E+ +L D+ G R G+ T
Sbjct: 728 TPSKLKYASEFDPFPCPPPYSCSKLDNDVTEIRNEHQTLVFDVKNGSLRKIVHRNGSETV 787
Query: 198 LLDQTGRYDSPEYANVDFKPDFK-----------VTS----LAEVYSILETNFELSP 239
+ ++ G Y SPE FKPD + VTS + EV+S +T +E SP
Sbjct: 788 VGEEIGMYSSPESGAYLFKPDGEAQPIVQPDGHVVTSEGLLVQEVFSYPKTKWEKSP 844
>At5g36790 p-nitrophenylphosphatase-like protein
Length = 362
Score = 32.7 bits (73), Expect = 0.18
Identities = 21/79 (26%), Positives = 36/79 (44%), Gaps = 7/79 (8%)
Query: 155 KPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCL----LDQTGRYDSPEY 210
KP + ++ + +Q +++ MVGD L DI G G T L + +SPE
Sbjct: 281 KPSTFMMDYLADKFGIQKSQICMVGDRLDTDILFGQNGGCKTLLVLSGVTSISMLESPEN 340
Query: 211 ANVDFKPDFKVTSLAEVYS 229
+PDF + +++ S
Sbjct: 341 ---KIQPDFYTSKISDFLS 356
>At5g36700 N-glyceraldehyde-2-phosphotransferase-like
Length = 289
Score = 32.7 bits (73), Expect = 0.18
Identities = 21/79 (26%), Positives = 36/79 (44%), Gaps = 7/79 (8%)
Query: 155 KPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCL----LDQTGRYDSPEY 210
KP + ++ + +Q +++ MVGD L DI G G T L + +SPE
Sbjct: 208 KPSTFMMDYLADKFGIQKSQICMVGDRLDTDILFGQNGGCKTLLVLSGVTSISMLESPEN 267
Query: 211 ANVDFKPDFKVTSLAEVYS 229
+PDF + +++ S
Sbjct: 268 ---KIQPDFYTSKISDFLS 283
>At5g13540 putative protein
Length = 788
Score = 30.0 bits (66), Expect = 1.1
Identities = 17/46 (36%), Positives = 26/46 (55%)
Query: 14 PRIMSSHLTPKTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGDDEYL 59
PR+ S+ TP T + + DMDGTL+ V ++++ GD E L
Sbjct: 357 PRLGWSNETPATIGKVMRIDMDGTLSAQVTGRQTLWRVSPGDAELL 402
>At5g47760 4-nitrophenylphosphatase-like protein
Length = 301
Score = 29.3 bits (64), Expect = 1.9
Identities = 19/81 (23%), Positives = 39/81 (47%), Gaps = 3/81 (3%)
Query: 155 KPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLL--DQTGRYDSPEYAN 212
KP + + + + + + MVGD L DI G AG T L+ T + + N
Sbjct: 221 KPSTFMMDFLLQKFGTETSRMCMVGDRLDTDILFGQNAGCKTLLVLTGVTSESNLLDKGN 280
Query: 213 VDFKPDFKVTSLAEVYSILET 233
+PD+ ++++++ ++E+
Sbjct: 281 -KIEPDYYTSTVSDIIKLMES 300
>At5g44730 Dreg-2 like protein
Length = 255
Score = 28.5 bits (62), Expect = 3.3
Identities = 11/32 (34%), Positives = 18/32 (55%)
Query: 170 VQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQ 201
+ P EV+ +GDS++ D G H L+D+
Sbjct: 190 IAPEEVLHIGDSMRKDYVPAKSIGMHALLVDR 221
>At4g11570 unknown protein
Length = 373
Score = 28.1 bits (61), Expect = 4.3
Identities = 22/84 (26%), Positives = 40/84 (47%), Gaps = 5/84 (5%)
Query: 102 QIMPGAADLCNVLDSKKIRRGLITRNMKSAVDLFHQRFGIT--FSPALSRE--FRPYKPD 157
++ G+ + NVL + KI L++ + ++ GI FS ++ E +R KPD
Sbjct: 212 RLRDGSQEFVNVLMNNKIPMALVSTRPRETLENAVGSIGIRKFFSVIVASEDVYRG-KPD 270
Query: 158 PAPLLHICSLWEVQPNEVIMVGDS 181
P ++ L + P I+ G+S
Sbjct: 271 PEMFIYAAQLLDFIPERCIVFGNS 294
>At3g58830 putative protein
Length = 348
Score = 28.1 bits (61), Expect = 4.3
Identities = 15/36 (41%), Positives = 19/36 (52%)
Query: 173 NEVIMVGDSLKDDIACGGRAGAHTCLLDQTGRYDSP 208
+E+IMVGD DI G R G T L + R + P
Sbjct: 270 SELIMVGDRPFTDIVYGNRNGFLTVLTEPLSRAEEP 305
>At1g06470 integral membrane protein, putative
Length = 414
Score = 28.1 bits (61), Expect = 4.3
Identities = 14/41 (34%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Query: 74 LIDHWSPLQQRQAYDTIAHFEKQALDHLQIMPGAADLCNVL 114
L+D WS + + +D+ AHF + L + GA C VL
Sbjct: 279 LLDPWSEFRDNKYFDSGAHFARTCF--LMLFGGALAFCMVL 317
>At3g18810 protein kinase, putative
Length = 700
Score = 27.7 bits (60), Expect = 5.7
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 4/42 (9%)
Query: 179 GDSLKDDIACGGRAGAHTCLLDQTGRYDSPEYANVDFKPDFK 220
GD+ DD++ GG+AG + L GR S +Y + + D K
Sbjct: 611 GDATLDDLSEGGKAGQSSFL----GRGSSSDYDSSTYSADMK 648
>At3g59530 unknown protein
Length = 403
Score = 27.3 bits (59), Expect = 7.4
Identities = 14/38 (36%), Positives = 18/38 (46%), Gaps = 6/38 (15%)
Query: 131 AVDLFHQRFGITFSPALSREFRPYKPDPAPLLHICSLW 168
A+D FH SP REF+P K + AP + W
Sbjct: 31 AIDPFHM------SPIGGREFKPVKHEVAPYKEVMGSW 62
>At2g39360 putative protein kinase
Length = 815
Score = 27.3 bits (59), Expect = 7.4
Identities = 17/62 (27%), Positives = 26/62 (41%)
Query: 5 SSTKAFLLFPRIMSSHLTPKTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGDDEYLRVKAS 64
+ T L+F + P ++ D D +L + V F +YK VL D + VK
Sbjct: 458 NETGESLIFSSSKIGYRYPLALIKEATDDFDESLVIGVGGFGKVYKGVLRDKTEVAVKRG 517
Query: 65 NP 66
P
Sbjct: 518 AP 519
>At5g12210 Rab geranylgeranyltransferase, beta subunit
Length = 321
Score = 26.9 bits (58), Expect = 9.7
Identities = 11/29 (37%), Positives = 18/29 (61%)
Query: 113 VLDSKKIRRGLITRNMKSAVDLFHQRFGI 141
+LD + + G I+ + AVD+FH FG+
Sbjct: 261 ILDCQDLDNGGISDRPEDAVDIFHTYFGV 289
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.139 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,550,841
Number of Sequences: 26719
Number of extensions: 234347
Number of successful extensions: 468
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 458
Number of HSP's gapped (non-prelim): 16
length of query: 239
length of database: 11,318,596
effective HSP length: 96
effective length of query: 143
effective length of database: 8,753,572
effective search space: 1251760796
effective search space used: 1251760796
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144807.2