BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.21 - phase: 0
(471 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g50200 alanine--tRNA ligase, putative 749 0.0
At5g22800 alanyl-tRNA synthetase 300 1e-81
At1g54560 35 0.068
At1g08730 myosin MYA1, class V (Z28389) like protein 35 0.12
At5g14940 oligopeptide transporter -like protein 33 0.34
At5g06140 sorting nexin-like protein 30 3.8
At2g44970 unknown protein 30 3.8
At3g13880 hypothetical protein 29 4.9
At5g43900 myosin heavy chain MYA2 (pir||S51824) 29 6.4
At4g28710 myosin heavy chain - like protein (fragment) 29 6.4
At2g27480 putative calcium binding protein 29 6.4
At1g04160 myosin heavy chain MYA2 29 6.4
At5g40820 AtRAD3 (dbj|BAA92828.1) 28 8.4
At4g33200 myosin - like protein 28 8.4
At2g31900 putative unconventional myosin 28 8.4
>At1g50200 alanine--tRNA ligase, putative
Length = 1027
Score = 749 bits (1933), Expect = 0.0
Identities = 355/462 (76%), Positives = 401/462 (85%)
Query: 1 MPETESIDIEWPAKRVRDTFAKFFEEKNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIFL 60
MP +E + +WPAKRVRDT+ FF K H W SS VVP NDPTLLFANAGMNQ+KPIFL
Sbjct: 49 MPGSEPSETQWPAKRVRDTYVDFFRGKGHKFWPSSPVVPHNDPTLLFANAGMNQYKPIFL 108
Query: 61 GTVDPNTALSKLTRACNIQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKEEA 120
GT DPNT LSKL+RACN QKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFK+EA
Sbjct: 109 GTADPNTELSKLSRACNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKKEA 168
Query: 121 ISWAWELLTEVYNLPSDRIYVTYFGGDEKTGLGPDNEARDIWLKFLPPGRVLPFGSKDNF 180
I WAWELLT+VY LP+DRIY TYFGGDEK GL PDNEARDIWLK LP GRVLPFG KDNF
Sbjct: 169 IEWAWELLTKVYGLPTDRIYATYFGGDEKAGLQPDNEARDIWLKVLPSGRVLPFGCKDNF 228
Query: 181 WEMGDTGPCGPCSEIHFDRIGNRNASSFVNDEDPNCIELWNLVFIQFNREADGSLKSLPA 240
WEMGDTGPCGPC+EIH+DRIGNR+A+S VN++DP C+E+WNLVFIQFNRE+DGSLK LPA
Sbjct: 229 WEMGDTGPCGPCTEIHYDRIGNRDAASLVNNDDPTCLEIWNLVFIQFNRESDGSLKPLPA 288
Query: 241 KHVDTGMGFERLTSILQHKNSNYDTDVFVPIFDAIQQATGARPYSGKVGPHDADKVDMAY 300
KHVDTGMGFERLTS+LQ+K SNYDTDVF+PIFD IQ+ATGARPYSGKVGP D D+VDMAY
Sbjct: 289 KHVDTGMGFERLTSVLQNKMSNYDTDVFMPIFDDIQKATGARPYSGKVGPEDVDRVDMAY 348
Query: 301 RVVADHIRTLSIAIADGSQPGNVGREYVLRRILRRAVRYGREVLKAEEGFFNGLVNVVAN 360
RVVADHIRTLS AIADGS+PGN GREYVLRRILRRAVRYG+E+LKAEEGFFNGLV+ V
Sbjct: 349 RVVADHIRTLSFAIADGSRPGNEGREYVLRRILRRAVRYGKEILKAEEGFFNGLVSSVIR 408
Query: 361 VLGDVFPELKQNEERIRKMIQVEEESFSRNLFKGIRKFNKAVEHVQGNILSGEATFELSH 420
V+GDVF ELK++E++I +I+ EE SF + L KGI KF KA + VQGN LSG+ F L
Sbjct: 409 VMGDVFTELKEHEKKITDIIKEEEASFCKTLAKGIEKFRKAGQAVQGNTLSGDDAFILWD 468
Query: 421 TFGFPLDLTQLMAEEKKFSVDIEGFHRAMKAARERSKTAPKK 462
T+GFPLDLTQLMAEE+ VD++GF++AM+ ARERS++A K
Sbjct: 469 TYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNK 510
>At5g22800 alanyl-tRNA synthetase
Length = 954
Score = 300 bits (767), Expect = 1e-81
Identities = 180/462 (38%), Positives = 257/462 (54%), Gaps = 42/462 (9%)
Query: 16 VRDTFAKFFEEKNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIFLGTVDPNTALSKLTRA 75
+R F +FF + H SS++VP DPT+L AGM QFKPIFLG V ++ A
Sbjct: 59 IRRRFLEFFASRGHKVLPSSSLVP-EDPTVLLTIAGMLQFKPIFLGKVP-----REVPCA 112
Query: 76 CNIQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKEEAISWAWELLTEVYNLP 135
Q+CIR NDL++VGK HHTFFEMLGN+SFGDYFK+EAI WAWEL T + LP
Sbjct: 113 TTAQRCIRT----NDLENVGKTARHHTFFEMLGNFSFGDYFKKEAIKWAWELSTIEFGLP 168
Query: 136 SDRIYVTYFGGDEKTGLGPDNEARDIWLKFLPPG--RVLPFGSKDNFWEMGDTGPCGPCS 193
++R++V+ + D+ EA +IW + R+ G DNFW G TGPCGPCS
Sbjct: 169 ANRVWVSIYEDDD--------EAFEIWKNEVGVSVERIKRMGEADNFWTSGPTGPCGPCS 220
Query: 194 EIHFDRIGNRNASSFVN-DEDPNCIELWNLVFIQFNREADGSLKSLPAKHVDTGMGFERL 252
E+++D R V+ +D IE +NLVF+Q+N+ DG L+ L K++DTG+G ER+
Sbjct: 221 ELYYDFYPERGYDEDVDLGDDTRFIEFYNLVFMQYNKTEDGLLEPLKQKNIDTGLGLERI 280
Query: 253 TSILQHKNSNYDTDVFVPIFDAIQQATGARPYSGKVGPHDADKVDMAYRVVADHIRTLSI 312
ILQ +NY+TD+ PI I + S DK + +V+ADH+R +
Sbjct: 281 AQILQKVPNNYETDLIYPIIAKISELANISYDSAN------DKAKTSLKVIADHMRAVVY 334
Query: 313 AIADGSQPGNVGREYVLRRILRRAVRYGREV-------LKAEEGFFNGLVNVVANVLGDV 365
I+DG P N+GR YV+RR++RRAVR G+ + + F + V + +
Sbjct: 335 LISDGVSPSNIGRGYVVRRLIRRAVRKGKSLGINGDMNGNLKGAFLPAVAEKVIELSTYI 394
Query: 366 FPELKQNEERIRKMIQVEEESFSRNLFKGIR----KFNKAVEHVQGN----ILSGEATFE 417
++K RI + I+ EE F + L +G + K N A+ L G+ F
Sbjct: 395 DSDVKLKASRIIEEIRQEELHFKKTLERGEKLLDQKLNDALSIADKTKDTPYLDGKDAFL 454
Query: 418 LSHTFGFPLDLTQLMAEEKKFSVDIEGFHRAMKAARERSKTA 459
L TFGFP+++T +AEE+ S+D+ GF M+ R +S+ A
Sbjct: 455 LYDTFGFPVEITAEVAEERGVSIDMNGFEVEMENQRRQSQAA 496
>At1g54560
Length = 1529
Score = 35.4 bits (80), Expect = 0.068
Identities = 37/134 (27%), Positives = 59/134 (43%), Gaps = 13/134 (9%)
Query: 271 IFDA--IQQATGARPYSGKVGPHDADKVDMAYRVVADHIRTLSIAIADGSQPGNVGREYV 328
I+DA +QQ GA P+ G++ PH D+AYR + + ++ SI ++ S G +
Sbjct: 114 IYDAHMMQQYKGA-PF-GELSPHVFAVADVAYRAMINEGKSNSILVSGESGAGKTETTKM 171
Query: 329 LRRILR----RAVRYGREVLKAEEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEE 384
L R L RAV GR V E+ N V G+ N R K ++++
Sbjct: 172 LMRYLAYLGGRAVTEGRTV---EQQVLES--NPVLEAFGNAKTVRNNNSSRFGKFVEIQF 226
Query: 385 ESFSRNLFKGIRKF 398
+ R +R +
Sbjct: 227 DKQGRISGAAVRTY 240
>At1g08730 myosin MYA1, class V (Z28389) like protein
Length = 1572
Score = 34.7 bits (78), Expect = 0.12
Identities = 37/134 (27%), Positives = 57/134 (41%), Gaps = 13/134 (9%)
Query: 271 IFDA--IQQATGARPYSGKVGPHDADKVDMAYRVVADHIRTLSIAIADGSQPGNVGREYV 328
I+DA +QQ GA G++ PH D+AYR + + ++ SI ++ S G +
Sbjct: 119 IYDAHMMQQYKGAP--LGELSPHVFAVADVAYRAMINEGKSNSILVSGESGAGKTETTKM 176
Query: 329 LRRILR----RAVRYGREVLKAEEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEE 384
L R L RAV GR V E+ N V G+ N R K ++++
Sbjct: 177 LMRYLAYLGGRAVTEGRTV---EQQVLES--NPVLEAFGNAKTVRNNNSSRFGKFVEIQF 231
Query: 385 ESFSRNLFKGIRKF 398
+ R IR +
Sbjct: 232 DKQGRISGAAIRTY 245
>At5g14940 oligopeptide transporter -like protein
Length = 547
Score = 33.1 bits (74), Expect = 0.34
Identities = 23/66 (34%), Positives = 32/66 (47%), Gaps = 4/66 (6%)
Query: 194 EIHFDRIGNRNASSFVNDEDPNCIEL----WNLVFIQFNREADGSLKSLPAKHVDTGMGF 249
EI +R+ RN + VND D N +EL + N EA+ + KSLP H GF
Sbjct: 239 EIIKERVCGRNKITLVNDHDLNAMELELQDQKPLCNCSNTEANTTTKSLPDDHKSCKTGF 298
Query: 250 ERLTSI 255
L ++
Sbjct: 299 SGLETV 304
>At5g06140 sorting nexin-like protein
Length = 402
Score = 29.6 bits (65), Expect = 3.8
Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 6/69 (8%)
Query: 338 RYGREVLKAEEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEEESFSRNLFKGIRK 397
R+ E ++ + VN +A + PEL+Q+E+ +R +Q +EE+ R F+
Sbjct: 100 RFSAEFIEMRRAALDIFVNRIA-----LHPELQQSED-LRTFLQADEETMDRFRFQETSI 153
Query: 398 FNKAVEHVQ 406
F K + +Q
Sbjct: 154 FKKPADLMQ 162
>At2g44970 unknown protein
Length = 503
Score = 29.6 bits (65), Expect = 3.8
Identities = 26/70 (37%), Positives = 34/70 (48%), Gaps = 9/70 (12%)
Query: 12 PAKRVRDTFAKFFEEKNH-VHWKSSAVVPLNDPTLLFANAGMNQFKPIFLGTVDPNTALS 70
P + D F K E+ H VH + VV L P L F+N G P++ VD T S
Sbjct: 185 PVEDGTDRFNKILEDIGHGVHRLPNTVVYLLVPGL-FSNHG-----PLYF--VDTKTKFS 236
Query: 71 KLTRACNIQK 80
K+ AC+I K
Sbjct: 237 KMGLACHIAK 246
>At3g13880 hypothetical protein
Length = 748
Score = 29.3 bits (64), Expect = 4.9
Identities = 17/46 (36%), Positives = 27/46 (57%), Gaps = 2/46 (4%)
Query: 227 FNREADGSLKSLPAKHVDTGMGFERLTSILQHKNSNYDTDVFVPIF 272
FN A SL +L K V G+G+ L+S+ Q KN+ D++ + +F
Sbjct: 11 FNNIAQDSLVTLITKRV--GLGYRFLSSLCQPKNTALDSEGYKILF 54
>At5g43900 myosin heavy chain MYA2 (pir||S51824)
Length = 1505
Score = 28.9 bits (63), Expect = 6.4
Identities = 29/117 (24%), Positives = 45/117 (37%), Gaps = 9/117 (7%)
Query: 286 GKVGPHDADKVDMAYRVVADHIRTLSIAIADGSQPGNVGREYVLRRILR----RAVRYGR 341
G++ PH D AYR + + + SI ++ S G +L R L RA GR
Sbjct: 126 GELSPHPFAVADAAYRQMINDGVSQSILVSGESGAGKTESTKLLMRYLAYMGGRAAAEGR 185
Query: 342 EVLKAEEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEEESFSRNLFKGIRKF 398
V E+ N V G+ N R K ++++ + R IR +
Sbjct: 186 SV---EQKVLES--NPVLEAFGNAKTVRNNNSSRFGKFVEIQFDEKGRISGAAIRTY 237
>At4g28710 myosin heavy chain - like protein (fragment)
Length = 1446
Score = 28.9 bits (63), Expect = 6.4
Identities = 33/128 (25%), Positives = 50/128 (38%), Gaps = 11/128 (8%)
Query: 275 IQQATGARPYSGKVGPHDADKVDMAYRVVADHIRTLSIAIADGSQPGNVGREYVLRRILR 334
+QQ GA G++ PH D AYR + + + SI ++ S G +L + L
Sbjct: 110 MQQYKGAG--FGELSPHPFAVADAAYRQMKNQGISQSILVSGESGAGKTETTKLLMQYLA 167
Query: 335 ----RAVRYGREVLKAEEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEEESFSRN 390
RAV GR V K N V G+ N R K ++++ + R
Sbjct: 168 DMGGRAVSEGRTVEKKVLE-----SNPVLEAFGNAKTVRNNNSSRFGKFVEIQFDQRGRI 222
Query: 391 LFKGIRKF 398
IR +
Sbjct: 223 SGAAIRTY 230
>At2g27480 putative calcium binding protein
Length = 186
Score = 28.9 bits (63), Expect = 6.4
Identities = 13/50 (26%), Positives = 24/50 (48%)
Query: 215 NCIELWNLVFIQFNREADGSLKSLPAKHVDTGMGFERLTSILQHKNSNYD 264
NC+ W +F +++R+ G + S + +G TS+ Q S +D
Sbjct: 79 NCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFD 128
>At1g04160 myosin heavy chain MYA2
Length = 1519
Score = 28.9 bits (63), Expect = 6.4
Identities = 29/118 (24%), Positives = 45/118 (37%), Gaps = 10/118 (8%)
Query: 286 GKVGPHDADKVDMAYRVVADHIRTLSIAIADGSQPGNVGREYVLRRIL-----RRAVRYG 340
G++ PH D AYR + + + SI ++ S G +L R L R A G
Sbjct: 126 GELSPHPFAVADAAYRQMVNEGVSQSILVSGESGAGKTESTKLLMRYLAFMGGRGAATEG 185
Query: 341 REVLKAEEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEEESFSRNLFKGIRKF 398
R V E+ N V G+ N R K ++++ + R IR +
Sbjct: 186 RTV---EQKVLES--NPVLEAFGNAKTVKNNNSSRFGKFVEIQFDQSGRISGAAIRTY 238
>At5g40820 AtRAD3 (dbj|BAA92828.1)
Length = 2810
Score = 28.5 bits (62), Expect = 8.4
Identities = 18/70 (25%), Positives = 35/70 (49%), Gaps = 2/70 (2%)
Query: 27 KNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIF--LGTVDPNTALSKLTRACNIQKCIRA 84
++HV KS ++ P + T +F NA ++ I+ L D + + L+++ N+Q +
Sbjct: 1665 ESHVRGKSGSLNPAAEKTGIFENADVSSLMGIYSCLDEPDGLSGFASLSKSLNLQDQLLI 1724
Query: 85 GGKHNDLDDV 94
K + DV
Sbjct: 1725 NKKSGNWADV 1734
>At4g33200 myosin - like protein
Length = 1374
Score = 28.5 bits (62), Expect = 8.4
Identities = 31/128 (24%), Positives = 54/128 (41%), Gaps = 11/128 (8%)
Query: 275 IQQATGARPYSGKVGPHDADKVDMAYRVVADHIRTLSIAIADGSQPGNVGREYVLRRILR 334
++Q GA P+ G++ PH D+AYR + D R+ SI ++ S G ++ + L
Sbjct: 115 MEQYMGA-PF-GELSPHVFAVSDVAYRAMIDDSRSQSILVSGESGAGKTETTKLIMQYLT 172
Query: 335 ----RAVRYGREVLKAEEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEEESFSRN 390
RA R V E+ N + G+ N R K ++++ ++ R
Sbjct: 173 FVGGRATDDDRSV---EQQVLES--NPLLEAFGNAKTVRNDNSSRFGKFVEIQFDTNGRI 227
Query: 391 LFKGIRKF 398
IR +
Sbjct: 228 SGAAIRTY 235
>At2g31900 putative unconventional myosin
Length = 1490
Score = 28.5 bits (62), Expect = 8.4
Identities = 25/102 (24%), Positives = 41/102 (39%), Gaps = 9/102 (8%)
Query: 286 GKVGPHDADKVDMAYRVVADHIRTLSIAIADGSQPGNVGREYVLRRILR----RAVRYGR 341
G++ PH D +YR + + R+ SI ++ S G +L R L R+ GR
Sbjct: 60 GELSPHLFAVADTSYRAMINEARSQSILVSGESGAGKTETTKMLMRYLAFMGGRSDTEGR 119
Query: 342 EVLKAEEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVE 383
V E+ N V G+ N R K ++++
Sbjct: 120 SV---EQQVLES--NPVLEAFGNAKTVKNNNSSRFGKFVEIQ 156
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.320 0.139 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,540,490
Number of Sequences: 26719
Number of extensions: 539476
Number of successful extensions: 1125
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1114
Number of HSP's gapped (non-prelim): 16
length of query: 471
length of database: 11,318,596
effective HSP length: 103
effective length of query: 368
effective length of database: 8,566,539
effective search space: 3152486352
effective search space used: 3152486352
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC144765.21