BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.19 - phase: 0
(146 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g19840 unknown protein 185 6e-48
At1g44910 splicing factor like protein 57 3e-09
At3g19670 unknown protein 49 9e-07
At5g02330 putative protein 32 0.12
At4g19187 unknown protein 30 0.35
At1g54560 30 0.35
At5g47680 unknown protein 30 0.45
At5g23210 serine carboxypeptidase II-like protein 30 0.59
At5g10800 putative protein 30 0.59
At2g26580 unknown protein 30 0.59
At4g36520 trichohyalin like protein 29 0.78
At4g38810 EF-Hand containing protein -like 29 1.0
At4g16310 hypothetical protein 28 1.3
At3g26560 ATP-dependent RNA helicase, putative 28 1.3
At3g63500 putative protein 28 2.3
At5g46020 unknown protein 27 2.9
At4g26130 uncharacterized protein 27 2.9
At2g02090 putative helicase 27 2.9
At5g49240 putative protein 27 3.9
At5g40450 unknown protein 27 3.9
>At3g19840 unknown protein
Length = 830
Score = 185 bits (470), Expect = 6e-48
Identities = 89/110 (80%), Positives = 98/110 (88%)
Query: 1 MLKERGVAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKA 60
MLKERG+APFSKWEKELPKI+FDPRFKAIPS+S RRSLFE YVK RAEEER+EKRAA KA
Sbjct: 460 MLKERGIAPFSKWEKELPKIIFDPRFKAIPSHSVRRSLFEQYVKTRAEEERREKRAAHKA 519
Query: 61 AIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDRKEREHLLNER 110
AIEGF+QLLD+AS DID TD F+KKWGND RFEA++RKERE LLNER
Sbjct: 520 AIEGFRQLLDDASTDIDQHTDYRAFKKKWGNDLRFEAIERKEREGLLNER 569
Score = 28.9 bits (63), Expect = 1.0
Identities = 37/167 (22%), Positives = 65/167 (38%), Gaps = 43/167 (25%)
Query: 1 MLKERGVAPFSKWEKELPKIVFDPRFKA-------------IPSYSARRSLFEHYVKNRA 47
ML+ER ++ S W K + +PR+++ I A + +H +K R
Sbjct: 595 MLREREISINSHWSKVKDSLRNEPRYRSVAHEDREVFYYEYIAELKAAQRGDDHEMKARD 654
Query: 48 EE---------------------ERKEKRAAQKAAIEGFKQLLDEASED-----IDDKTD 81
EE ER ++ +K A ++ LL E D + K
Sbjct: 655 EEDKLRERERELRKRKEREVQEVERVRQKIRRKEASSSYQALLVEKIRDPEASWTESKPI 714
Query: 82 SHTFRKKWGNDPRFEALDRKE--REHL--LNERCHFEFNWTFLNSLN 124
+K ++P E D+++ R+H+ L ERC +F +L+
Sbjct: 715 LERDPQKRASNPDLEPADKEKLFRDHVKSLYERCVHDFKALLAEALS 761
>At1g44910 splicing factor like protein
Length = 958
Score = 57.4 bits (137), Expect = 3e-09
Identities = 33/97 (34%), Positives = 54/97 (55%), Gaps = 2/97 (2%)
Query: 13 WEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEA 72
WE+ L +IV D R+ A+ + R+ F Y+ R + E +E+R QK A E F ++L+E
Sbjct: 426 WEQTLKEIVHDKRYGALRTLGERKQAFNEYLGQRKKVEAEERRRRQKKAREEFVKMLEEC 485
Query: 73 SEDIDDKTDSHTFRKKWGNDPRFEALDR-KEREHLLN 108
E++ + ND RF+A+DR ++RE L +
Sbjct: 486 -EELSSSLKWSKAMSLFENDQRFKAVDRPRDREDLFD 521
Score = 37.0 bits (84), Expect = 0.004
Identities = 22/92 (23%), Positives = 42/92 (44%), Gaps = 1/92 (1%)
Query: 12 KWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLDE 71
KW K + D RFKA+ R LF++Y+ +ER++ + + +++ L E
Sbjct: 493 KWSKAMSLFENDQRFKAVDRPRDREDLFDNYIVELERKEREKAAEEHRQYMADYRKFL-E 551
Query: 72 ASEDIDDKTDSHTFRKKWGNDPRFEALDRKER 103
+ I T + + +D R L++ +R
Sbjct: 552 TCDYIKAGTQWRKIQDRLEDDDRCSCLEKIDR 583
Score = 28.9 bits (63), Expect = 1.0
Identities = 19/94 (20%), Positives = 41/94 (43%), Gaps = 4/94 (4%)
Query: 11 SKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLD 70
S WE + +++I S + LFE Y+ + E+ ++++R + + K+ +
Sbjct: 767 SNWEDSKQLVEESQEYRSIGDESVSQGLFEEYITSLQEKAKEKERKRDEEKVRKEKERDE 826
Query: 71 EASEDIDDKTDSHTFRKKWGNDPRFEALDRKERE 104
+ DK R+K + + +R +RE
Sbjct: 827 KEKRKDKDKER----REKEREREKEKGKERSKRE 856
Score = 26.6 bits (57), Expect = 5.0
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 6/58 (10%)
Query: 48 EEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDRKEREH 105
+E+RK K +K ++ S D DD+ +S +K GND +K R+H
Sbjct: 872 DEKRKGKDRDRKHRRRHHNNSDEDVSSDRDDRDESKKSSRKHGND------RKKSRKH 923
>At3g19670 unknown protein
Length = 960
Score = 48.9 bits (115), Expect = 9e-07
Identities = 31/110 (28%), Positives = 55/110 (49%), Gaps = 2/110 (1%)
Query: 1 MLKERGVAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKA 60
+LK V WE+ + +I+ D R+ A+ + R+ F ++ +E+ A QK
Sbjct: 428 LLKSAKVGSDWTWEQAMREIINDKRYGALRTLGERKQAFNEFLLQTKRAAEEERLARQKK 487
Query: 61 AIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDR-KEREHLLNE 109
E FK++L+E E S T + +D RF+AL+R K+R ++ +
Sbjct: 488 LYEDFKRMLEECVELTPSTRWSKTV-TMFEDDERFKALEREKDRRNIFED 536
Score = 37.4 bits (85), Expect = 0.003
Identities = 19/67 (28%), Positives = 34/67 (50%)
Query: 7 VAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEGFK 66
+ P ++W K + D RFKA+ RR++FE +V E+ R + +K I +K
Sbjct: 502 LTPSTRWSKTVTMFEDDERFKALEREKDRRNIFEDHVSELKEKGRVKALEDRKRNIIEYK 561
Query: 67 QLLDEAS 73
+ L+ +
Sbjct: 562 RFLESCN 568
Score = 28.9 bits (63), Expect = 1.0
Identities = 25/101 (24%), Positives = 48/101 (46%), Gaps = 10/101 (9%)
Query: 7 VAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKN--RAEEERK-----EKRAAQK 59
+ P S+W K ++ D R + + +F+ Y+++ R EEE+K E + ++
Sbjct: 570 IKPNSQWRKVQDRLEVDERCSRLEKID-QLEIFQEYLRDLEREEEEKKKIQKEELKKVER 628
Query: 60 AAIEGFKQLLDE--ASEDIDDKTDSHTFRKKWGNDPRFEAL 98
+ F LLDE A+ ++ KT + K + P + A+
Sbjct: 629 KHRDEFHGLLDEHIATGELTAKTIWRDYLMKVKDLPVYSAI 669
>At5g02330 putative protein
Length = 656
Score = 32.0 bits (71), Expect = 0.12
Identities = 23/75 (30%), Positives = 34/75 (44%), Gaps = 1/75 (1%)
Query: 64 GFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDRKEREHLLNERCHFEFNWTFLNSL 123
GF D+ S DI + S F + P F+ALDRKE + + C ++ LN +
Sbjct: 464 GFIYEEDQYSIDIRCASVSEPFDYQGHEHPLFQALDRKEEREAICQICRKKYG-RKLNCI 522
Query: 124 NFIFFVCDSHNHHPY 138
+ +C S PY
Sbjct: 523 ECNYIICFSCATLPY 537
>At4g19187 unknown protein
Length = 595
Score = 30.4 bits (67), Expect = 0.35
Identities = 23/84 (27%), Positives = 46/84 (54%), Gaps = 6/84 (7%)
Query: 32 YSARRSLF---EHYVKNRAEEE-RKEKRAAQK--AAIEGFKQLLDEASEDIDDKTDSHTF 85
Y RR + E +NR+E++ R + R ++K +++G ++ + EAS+D ++ +D +
Sbjct: 494 YDERRHRYVDMESENRNRSEKKPRYDDRDSEKHHRSVKGKEKHVYEASDDPEEFSDRYRS 553
Query: 86 RKKWGNDPRFEALDRKEREHLLNE 109
KK +D RK++ L +E
Sbjct: 554 TKKTESDSESNRRSRKKKHELSSE 577
>At1g54560
Length = 1529
Score = 30.4 bits (67), Expect = 0.35
Identities = 20/64 (31%), Positives = 30/64 (46%)
Query: 49 EERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDRKEREHLLN 108
E E+ KA +E KQ D+A+ D+ +S RKK D +A +E L
Sbjct: 979 EALTEEVEGLKANLEQEKQRADDATRKFDEAQESSEDRKKKLEDTEKKAQQLQESVTRLE 1038
Query: 109 ERCH 112
E+C+
Sbjct: 1039 EKCN 1042
>At5g47680 unknown protein
Length = 344
Score = 30.0 bits (66), Expect = 0.45
Identities = 19/68 (27%), Positives = 34/68 (49%), Gaps = 3/68 (4%)
Query: 3 KERGVAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAI 62
++ G +WE+ L + R K I S R+SL + ++ R+EE+ K+ +A
Sbjct: 59 RKEGERKLKEWEETLANATEEERLKLIES---RKSLRKERMEKRSEEKEKKIERLNQAKE 115
Query: 63 EGFKQLLD 70
G K ++D
Sbjct: 116 IGQKIVVD 123
>At5g23210 serine carboxypeptidase II-like protein
Length = 499
Score = 29.6 bits (65), Expect = 0.59
Identities = 26/92 (28%), Positives = 40/92 (43%), Gaps = 7/92 (7%)
Query: 24 PRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSH 83
P++K+ Y A S HYV +E KE + A K K L+ + +DD+TD
Sbjct: 187 PQYKSHDFYIAGESYAGHYVPQLSELIYKENKIASKKDFINLKGLM-IGNALLDDETDQK 245
Query: 84 -TFRKKWGNDPRFEALDRKEREHLLNERCHFE 114
W + +AL K +N+ C F+
Sbjct: 246 GMIEYAWDHAVISDALYEK-----VNKNCDFK 272
>At5g10800 putative protein
Length = 957
Score = 29.6 bits (65), Expect = 0.59
Identities = 24/87 (27%), Positives = 41/87 (46%), Gaps = 9/87 (10%)
Query: 35 RRSLFEHYVKNRAEEERK---EKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGN 91
R SL E +KN E ERK ++ + + G +++L E E +D DS R + +
Sbjct: 827 RESLEEQGMKNSEEIERKVAIHRKRLEADGLSGNQRVLPEKREKREDSRDSSRKRNRSES 886
Query: 92 DPRFEALDRKE------REHLLNERCH 112
R ++ +K R+H L++ H
Sbjct: 887 QNRSQSPPQKSLTRERVRDHDLDKDRH 913
>At2g26580 unknown protein
Length = 114
Score = 29.6 bits (65), Expect = 0.59
Identities = 18/65 (27%), Positives = 30/65 (45%)
Query: 29 IPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKK 88
IPS + R++ E + NR E+R+ +A I+ Q + + DI + T K
Sbjct: 33 IPSRISTRTITEQRIVNRPPEKRQRVPSAYNQFIKEEIQRIKANNPDISHREAFSTAAKN 92
Query: 89 WGNDP 93
W + P
Sbjct: 93 WAHFP 97
>At4g36520 trichohyalin like protein
Length = 1432
Score = 29.3 bits (64), Expect = 0.78
Identities = 21/71 (29%), Positives = 35/71 (48%), Gaps = 1/71 (1%)
Query: 40 EHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRF-EAL 98
E +K R E E E+RA + ++ L EA E +++ R+K N + EA+
Sbjct: 776 ERQIKERQEREENERRAKEVLEQAENERKLKEALEQKENERRLKETREKEENKKKLREAI 835
Query: 99 DRKEREHLLNE 109
+ +E+E L E
Sbjct: 836 ELEEKEKRLIE 846
Score = 27.7 bits (60), Expect = 2.3
Identities = 20/83 (24%), Positives = 36/83 (43%), Gaps = 6/83 (7%)
Query: 40 EHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEASEDID------DKTDSHTFRKKWGNDP 93
E +K E+E +K+ + +E ++ L EA E + + + R +
Sbjct: 815 ERRLKETREKEENKKKLREAIELEEKEKRLIEAFERAEIERRLKEDLEQEEMRMRLQEAK 874
Query: 94 RFEALDRKEREHLLNERCHFEFN 116
E L R+ +EH NER E++
Sbjct: 875 ERERLHRENQEHQENERKQHEYS 897
>At4g38810 EF-Hand containing protein -like
Length = 375
Score = 28.9 bits (63), Expect = 1.0
Identities = 9/32 (28%), Positives = 25/32 (78%)
Query: 48 EEERKEKRAAQKAAIEGFKQLLDEASEDIDDK 79
EE+++EK A+Q+ +E FK++++ ++ ++++
Sbjct: 198 EEDKREKSASQERFLEAFKRVVESVAQRLNEQ 229
>At4g16310 hypothetical protein
Length = 1265
Score = 28.5 bits (62), Expect = 1.3
Identities = 22/62 (35%), Positives = 30/62 (47%), Gaps = 2/62 (3%)
Query: 44 KNRAEEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDRKER 103
K++ E E K+ QK +E L +ASED+DD S R K GN E+ + R
Sbjct: 43 KDKVETESTGKQR-QKQVVEKKLSALGKASEDMDDTLASFRKRLK-GNKKGVESGTSRVR 100
Query: 104 EH 105
H
Sbjct: 101 NH 102
>At3g26560 ATP-dependent RNA helicase, putative
Length = 1168
Score = 28.5 bits (62), Expect = 1.3
Identities = 25/87 (28%), Positives = 39/87 (44%), Gaps = 5/87 (5%)
Query: 23 DPRFK--AIPSYSARRSLFEHYVKNRAEEERKEK-RAAQKAAIEGFKQLLDEASEDIDDK 79
D +FK AI + E ++ AEE R+E+ R + E + + + D DD+
Sbjct: 92 DQKFKGLAIKDTKDKVKELEKEIEREAEERRREEDRNRDRDRRESGRDRDRDRNRDRDDR 151
Query: 80 TDSHTFRKKWGNDPRFE--ALDRKERE 104
D H R++ D E DR+ RE
Sbjct: 152 RDRHRDRERNRGDEEGEDRRSDRRHRE 178
>At3g63500 putative protein
Length = 1162
Score = 27.7 bits (60), Expect = 2.3
Identities = 19/91 (20%), Positives = 41/91 (44%), Gaps = 3/91 (3%)
Query: 14 EKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEAS 73
E+ PK +PRF+ + S + + RA++ R+E ++ AI +++ +E +
Sbjct: 1047 ERGSPK---EPRFEELESIVRMKQAEAEMFQGRADDARREAEGLKRIAIAKKEKIEEEYN 1103
Query: 74 EDIDDKTDSHTFRKKWGNDPRFEALDRKERE 104
+ + ++ EA+ R +RE
Sbjct: 1104 RRMGKLSMEDAQERRRRRYEELEAMQRGQRE 1134
>At5g46020 unknown protein
Length = 164
Score = 27.3 bits (59), Expect = 2.9
Identities = 19/82 (23%), Positives = 38/82 (46%), Gaps = 2/82 (2%)
Query: 31 SYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWG 90
S+ + + +E V+ +EEE +E+ + + + + E D ++ T + K
Sbjct: 33 SFKQKEAEYEEDVEEESEEESEEESEDEADVKKKGAEAVIEV--DNPNRVRQKTLKAKDL 90
Query: 91 NDPRFEALDRKEREHLLNERCH 112
+ + L R+ERE L +R H
Sbjct: 91 DASKTTELSRREREELEKQRAH 112
>At4g26130 uncharacterized protein
Length = 286
Score = 27.3 bits (59), Expect = 2.9
Identities = 22/78 (28%), Positives = 35/78 (44%), Gaps = 6/78 (7%)
Query: 36 RSLFEHYVKNRAEE--ERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDP 93
+S E ++ EE E EKR + +E + D E +DDK + F K+
Sbjct: 211 KSASERHIGREEEETVEAVEKRRPETMRVERTTSIGDGGEEGVDDKASN--FINKFKQQL 268
Query: 94 RFEALDR--KEREHLLNE 109
+ + LD + RE L N+
Sbjct: 269 KLQRLDSFLRYREMLKND 286
>At2g02090 putative helicase
Length = 763
Score = 27.3 bits (59), Expect = 2.9
Identities = 15/45 (33%), Positives = 27/45 (59%), Gaps = 2/45 (4%)
Query: 48 EEERKEKRAAQKAAIEGFKQLLD--EASEDIDDKTDSHTFRKKWG 90
EEE +E + +AA G + +++ EAS+D DD+ +S ++G
Sbjct: 92 EEEVEETKVVMRAARVGRRFVIEDEEASDDDDDEAESSASEDEFG 136
>At5g49240 putative protein
Length = 292
Score = 26.9 bits (58), Expect = 3.9
Identities = 24/97 (24%), Positives = 40/97 (40%), Gaps = 10/97 (10%)
Query: 36 RSLFEHYVKNRAEEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRF 95
R +F+H VK E A+KAA E + D + + S + + R
Sbjct: 151 RIVFKHLVKKMRERRSVVTGEAEKAAGEKSSSVGDSTIRNPNKSKRSSCLEAEVNEEDRH 210
Query: 96 EALDR------KEREHLLNERCHFEFNWTFLNSLNFI 126
+ DR K+R + +E H FLN+++F+
Sbjct: 211 DHNDRACASSAKKRRVVWDEELH----QNFLNAVDFL 243
>At5g40450 unknown protein
Length = 2910
Score = 26.9 bits (58), Expect = 3.9
Identities = 13/43 (30%), Positives = 26/43 (60%), Gaps = 6/43 (13%)
Query: 48 EEERKEKRAA------QKAAIEGFKQLLDEASEDIDDKTDSHT 84
EE+ +E+R+ QK +E ++L+E+S+ +D+K + T
Sbjct: 922 EEQPQEERSVIDPTPLQKPTLESPSEVLEESSKTVDEKIEEKT 964
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.137 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,600,310
Number of Sequences: 26719
Number of extensions: 148953
Number of successful extensions: 707
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 24
Number of HSP's that attempted gapping in prelim test: 670
Number of HSP's gapped (non-prelim): 59
length of query: 146
length of database: 11,318,596
effective HSP length: 90
effective length of query: 56
effective length of database: 8,913,886
effective search space: 499177616
effective search space used: 499177616
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC144765.19