BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144765.18 - phase: 2 /pseudo
         (374 letters)

Database: ara_mips 
           26,719 sequences; 11,318,596 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

At1g50200 alanine--tRNA ligase, putative                              492  e-139
At5g22800 alanyl-tRNA synthetase                                      163  2e-40
At5g07450 unknown protein                                              30  2.1
At3g25560 unknown protein                                              28  6.2
At3g29638 putative protein                                             28  8.1

>At1g50200 alanine--tRNA ligase, putative
          Length = 1027

 Score =  492 bits (1267), Expect = e-139
 Identities = 251/354 (70%), Positives = 282/354 (78%), Gaps = 24/354 (6%)

Query: 27  NNDDPTCIEIWNLVLXQFNREADSSLKSLPAKHVDTGMGFERLTSILQRKLSNYDTDVFM 86
           NNDDPTC+EIWNLV  QFNRE+D SLK LPAKHVDTGMGFERLTS+LQ K+SNYDTDVFM
Sbjct: 258 NNDDPTCLEIWNLVFIQFNRESDGSLKPLPAKHVDTGMGFERLTSVLQNKMSNYDTDVFM 317

Query: 87  PIFDAIQLATGARPYSGKVGPEDADKIDMAYRVVADHIRTLSFAIADGSRPGNEGREYVL 146
           PIFD IQ ATGARPYSGKVGPED D++DMAYRVVADHIRTLSFAIADGSRPGNEGREYVL
Sbjct: 318 PIFDDIQKATGARPYSGKVGPEDVDRVDMAYRVVADHIRTLSFAIADGSRPGNEGREYVL 377

Query: 147 RRILRRAVRYGREVLKAEEGFFNGLVNVVANVLGDVFPELKQQEVHIRNVIQEEEESFGR 206
           RRILRRAVRYG+E+LKAEEGFFNGLV+ V  V+GDVF ELK+ E  I ++I+EEE SF +
Sbjct: 378 RRILRRAVRYGKEILKAEEGFFNGLVSSVIRVMGDVFTELKEHEKKITDIIKEEEASFCK 437

Query: 207 TLVKGIAKFKTAVGHVQGNILSGEATFELWDTYGFPLDLTQLMAEEKKLKVDVEGFNSAM 266
           TL KGI KF+ A   VQGN LSG+  F LWDTYGFPLDLTQLMAEE+ L VDV+GFN AM
Sbjct: 438 TLAKGIEKFRKAGQAVQGNTLSGDDAFILWDTYGFPLDLTQLMAEERGLLVDVDGFNKAM 497

Query: 267 EAARERSRTAQNKQAGGAIVMDADATSALQKRSIVPTDDSFKFASFR------------- 313
           E ARERSR+AQNKQAGGAIVMDADATS L K  +  TDDSFK+  F+             
Sbjct: 498 EEARERSRSAQNKQAGGAIVMDADATSTLHKAGVSATDDSFKYIWFQVCPFIPENLHVAP 557

Query: 314 -----------DHETVVKAIYTGSEFVDSVNTDGDVGVILESTSFYAEQGGQVF 356
                      DHE+ +KAIYTGS F++S     +VG++L STSFYAEQGGQ+F
Sbjct: 558 EMFLCWRRNEEDHESELKAIYTGSTFLESSAASDNVGLVLGSTSFYAEQGGQIF 611


>At5g22800 alanyl-tRNA synthetase
          Length = 954

 Score =  163 bits (412), Expect = 2e-40
 Identities = 111/347 (31%), Positives = 171/347 (48%), Gaps = 39/347 (11%)

Query: 29  DDPTCIEIWNLVLXQFNREADSSLKSLPAKHVDTGMGFERLTSILQRKLSNYDTDVFMPI 88
           DD   IE +NLV  Q+N+  D  L+ L  K++DTG+G ER+  ILQ+  +NY+TD+  PI
Sbjct: 240 DDTRFIEFYNLVFMQYNKTEDGLLEPLKQKNIDTGLGLERIAQILQKVPNNYETDLIYPI 299

Query: 89  FDAIQLATGARPYSGKVGPEDADKIDMAYRVVADHIRTLSFAIADGSRPGNEGREYVLRR 148
              I         S        DK   + +V+ADH+R + + I+DG  P N GR YV+RR
Sbjct: 300 IAKISELANISYDSAN------DKAKTSLKVIADHMRAVVYLISDGVSPSNIGRGYVVRR 353

Query: 149 ILRRAVRYGREVLKAEEGFFNG------LVNVVANVL--------------GDVFPELKQ 188
           ++RRAVR G+ +     G  NG      L  V   V+                +  E++Q
Sbjct: 354 LIRRAVRKGKSL--GINGDMNGNLKGAFLPAVAEKVIELSTYIDSDVKLKASRIIEEIRQ 411

Query: 189 QEVHIRNVIQEEEESFGRTLVKGIAKFKTAVGHVQGNILSGEATFELWDTYGFPLDLTQL 248
           +E+H +  ++  E+   + L   ++    A        L G+  F L+DT+GFP+++T  
Sbjct: 412 EELHFKKTLERGEKLLDQKLNDALS---IADKTKDTPYLDGKDAFLLYDTFGFPVEITAE 468

Query: 249 MAEEKKLKVDVEGFNSAMEAARERSRTAQNKQAGGAIVMDADATSALQKRSIVPTDDSFK 308
           +AEE+ + +D+ GF   ME  R +S+ A N      +  DAD T  +        D  F 
Sbjct: 469 VAEERGVSIDMNGFEVEMENQRRQSQAAHN-VVKLTVEDDADMTKNI-------ADTEFL 520

Query: 309 FASFRDHETVVKAIYTGSEFVDSVNTDGDVGVILESTSFYAEQGGQV 355
                    VVK++    + V  V+   +V V+L+ T FYAE GGQ+
Sbjct: 521 GYDSLSARAVVKSLLVNGKPVIRVSEGSEVEVLLDRTPFYAESGGQI 567


>At5g07450 unknown protein
          Length = 216

 Score = 30.0 bits (66), Expect = 2.1
 Identities = 19/80 (23%), Positives = 37/80 (45%), Gaps = 15/80 (18%)

Query: 295 LQKRSIVPTDDSFKFASFRDHETVVKAIYTGSEFVDSVNTDGDVGVILESTSFYAEQGGQ 354
           +QK+ ++P D S        H  ++ ++   ++F+D          +  + +FYA+ GG 
Sbjct: 93  IQKQPLLPIDSS------NVHRLIITSVLVSAKFMDD---------LCYNNAFYAKVGGI 137

Query: 355 VFSIVNILYMIFCMSILFYL 374
               +N+L + F   I F L
Sbjct: 138 TTEEMNLLELDFLFGIGFQL 157


>At3g25560 unknown protein
          Length = 635

 Score = 28.5 bits (62), Expect = 6.2
 Identities = 23/81 (28%), Positives = 39/81 (47%), Gaps = 7/81 (8%)

Query: 176 ANVLGDVFPELKQQEVHIRNVIQEEEESFGRTLVKGIAKFKTAVGHVQGNILSGEATFEL 235
           AN+L D + E    +  +  ++ + EES   T V+G       VGH+    LS   + E 
Sbjct: 439 ANILLDDYFEAVVGDFGLAKLL-DHEESHVTTAVRG------TVGHIAPEYLSTGQSSEK 491

Query: 236 WDTYGFPLDLTQLMAEEKKLK 256
            D +GF + L +L+   + L+
Sbjct: 492 TDVFGFGILLLELITGLRALE 512


>At3g29638 putative protein
          Length = 412

 Score = 28.1 bits (61), Expect = 8.1
 Identities = 28/97 (28%), Positives = 44/97 (44%), Gaps = 14/97 (14%)

Query: 183 FPELKQQEVHIRNVIQEEEESFGRTLVKGIAKFKTAVGHVQGNILSGEATFELWDTY--- 239
           F   ++ E H  +V+  EEESF       I  F T V      I+S +A FE ++ Y   
Sbjct: 325 FRNYREVEKHGESVMLSEEESF------KIDYFYTIVDQA---IVSLQARFEQFEEYGKH 375

Query: 240 -GFPLDLTQLMAE-EKKLKVDVEGFNSAMEAARERSR 274
            GF +DL +L  E E  LK       ++++  +   +
Sbjct: 376 FGFLIDLRKLKRETEDGLKASCINLETSLKHGKNSDK 412


  Database: ara_mips
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,978,382
  Number of sequences in database:  6832
  
  Database: /data/blast2/ara_mips_chr2
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,737,135
  Number of sequences in database:  4184
  
  Database: /data/blast2/ara_mips_chr3
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,236,886
  Number of sequences in database:  5377
  
  Database: /data/blast2/ara_mips_chr4
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,748,816
  Number of sequences in database:  4030
  
  Database: /data/blast2/ara_mips_chr5
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,569,679
  Number of sequences in database:  6098
  
  Database: /data/blast2/ara_mips_chl
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 25,951
  Number of sequences in database:  85
  
  Database: /data/blast2/ara_mips_mit
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 21,747
  Number of sequences in database:  113
  
Lambda     K      H
   0.322    0.138    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,130,696
Number of Sequences: 26719
Number of extensions: 335963
Number of successful extensions: 789
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 783
Number of HSP's gapped (non-prelim): 5
length of query: 374
length of database: 11,318,596
effective HSP length: 101
effective length of query: 273
effective length of database: 8,619,977
effective search space: 2353253721
effective search space used: 2353253721
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)