BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.13 + phase: 0
(146 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g07860 unknown protein 172 4e-44
At5g25340 unknown protein 71 2e-13
At4g32265 unknown protein 68 2e-12
At1g80060 hypothetical protein 52 1e-07
At3g26920 unknown protein 32 0.16
At2g07730 putative non-LTR retroelement reverse transcriptase 28 2.3
At1g55660 hypothetical protein 28 2.3
At5g18670 beta-amylase-like proten 27 2.9
At5g65600 receptor protein kinase-like protein 27 3.9
At4g00320 27 3.9
At5g64760 proteasome regulatory subunit-like 27 5.0
At5g55860 myosin heavy chain-like 27 5.0
At5g13680 putative protein 27 5.0
At4g20010 unknown protein 27 5.0
At3g19720 hypothetical protein 27 5.0
At1g43880 hypothetical protein 27 5.0
At4g01920 putative CHP-rich zinc finger protein 26 6.6
At1g53700 auxin-induced protein kinase like protein 26 6.6
At5g04230 phenylalanine ammonia-lyase PAL3 26 8.6
At4g33170 putative protein 26 8.6
>At3g07860 unknown protein
Length = 165
Score = 172 bits (437), Expect = 4e-44
Identities = 86/134 (64%), Positives = 108/134 (80%), Gaps = 3/134 (2%)
Query: 3 EGAEYKSGLKKAK---VLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDT 59
E +Y K+ K VL LL DPILADVP+NPTL DV TL+ LE GSAMR++V+KLD
Sbjct: 2 ESGDYDVETKREKLKSVLSQLLADPILADVPRNPTLSDVVTLVSLEKGSAMRLSVVKLDG 61
Query: 60 TSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDD 119
+S DV +MN+ATLKDLKL IKKKVN MEQ++MGHRHISW+ VW+N+CLS +N KLL+D+
Sbjct: 62 SSLDVAVMNSATLKDLKLLIKKKVNEMEQANMGHRHISWKHVWSNFCLSCNNEKLLDDNA 121
Query: 120 VLQNLGVRNNSQVS 133
VLQ++G+RNNSQV+
Sbjct: 122 VLQDVGIRNNSQVT 135
>At5g25340 unknown protein
Length = 208
Score = 70.9 bits (172), Expect = 2e-13
Identities = 33/83 (39%), Positives = 55/83 (65%), Gaps = 2/83 (2%)
Query: 50 MRITVLKLDTTSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSF 109
+R++VLKLD +SFDV ++ +AT+ DLK+AI+ +++ + G ISW VW ++CL F
Sbjct: 19 IRLSVLKLDGSSFDVYVLTSATVGDLKVAIETAFSHVPKK--GPSKISWSHVWGHFCLCF 76
Query: 110 DNNKLLNDDDVLQNLGVRNNSQV 132
KL+ D D + N G+++ +V
Sbjct: 77 GGQKLITDTDCIGNYGMKDGDEV 99
>At4g32265 unknown protein
Length = 239
Score = 68.2 bits (165), Expect = 2e-12
Identities = 29/83 (34%), Positives = 56/83 (66%), Gaps = 2/83 (2%)
Query: 50 MRITVLKLDTTSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSF 109
+++TVLKLD +SF + ++ TAT+ +LK+A++ +++ S G ISW VW +CLS+
Sbjct: 38 IKLTVLKLDGSSFGIQVLKTATVGELKMAVEAAFSHLPISGPGK--ISWPHVWGQFCLSY 95
Query: 110 DNNKLLNDDDVLQNLGVRNNSQV 132
++ +L+N+ + L G+++ Q+
Sbjct: 96 EDKRLINESEYLTEFGIKDGDQL 118
>At1g80060 hypothetical protein
Length = 243
Score = 51.6 bits (122), Expect = 1e-07
Identities = 24/83 (28%), Positives = 49/83 (58%), Gaps = 2/83 (2%)
Query: 50 MRITVLKLDTTSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSF 109
++++V+KL+ + FDV + ++ +LK A+++ GH ISW VW ++CL +
Sbjct: 53 IKLSVVKLNGSLFDVEVAKDCSVAELKRAVEQVFTISPLE--GHGMISWSHVWGHFCLCY 110
Query: 110 DNNKLLNDDDVLQNLGVRNNSQV 132
+ +L+ND ++ LG+ + Q+
Sbjct: 111 RDQRLVNDKTSIRYLGLNDGDQL 133
>At3g26920 unknown protein
Length = 340
Score = 31.6 bits (70), Expect = 0.16
Identities = 19/49 (38%), Positives = 27/49 (54%), Gaps = 3/49 (6%)
Query: 32 NPTLEDVETLIGLELGSAMRITVLKL---DTTSFDVILMNTATLKDLKL 77
+P + D E LIG+ G +R VLK+ D F L N+ TL+ L+L
Sbjct: 6 DPRIMDFEILIGIAFGRQLRKLVLKVYSGDWFKFPTSLYNSETLETLEL 54
>At2g07730 putative non-LTR retroelement reverse transcriptase
Length = 970
Score = 27.7 bits (60), Expect = 2.3
Identities = 20/65 (30%), Positives = 32/65 (48%)
Query: 38 VETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHIS 97
V + I + + SA+ + V LD+ + +L + TL L+ K KV M+ S+ S
Sbjct: 491 VLSTIPVHVMSAIILPVATLDSLDWWRLLHDKETLWARVLSKKYKVGEMQNSNWLKPKSS 550
Query: 98 WRSVW 102
W S W
Sbjct: 551 WSSTW 555
>At1g55660 hypothetical protein
Length = 492
Score = 27.7 bits (60), Expect = 2.3
Identities = 24/68 (35%), Positives = 33/68 (48%), Gaps = 8/68 (11%)
Query: 8 KSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILM 67
KS K ++ L+D IL DVP+ L ++TL LG R+T D S +L
Sbjct: 178 KSLCKCKSIVILKLKDEILVDVPRKVCLPSLKTLF---LG---RVTY--SDANSLHRLLS 229
Query: 68 NTATLKDL 75
N L+DL
Sbjct: 230 NCPVLEDL 237
>At5g18670 beta-amylase-like proten
Length = 536
Score = 27.3 bits (59), Expect = 2.9
Identities = 18/54 (33%), Positives = 26/54 (47%), Gaps = 7/54 (12%)
Query: 24 PILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKDLKL 77
P L PK+ +LE V+ +GL L + D + + + TA LK LKL
Sbjct: 73 PFLKSTPKSKSLESVKLFVGLPLDTVS-------DCNNVNHLKAITAGLKALKL 119
>At5g65600 receptor protein kinase-like protein
Length = 675
Score = 26.9 bits (58), Expect = 3.9
Identities = 14/32 (43%), Positives = 19/32 (58%), Gaps = 2/32 (6%)
Query: 111 NNKLLNDDDVLQNLGVRNNSQVSSTYLCHCWN 142
NN + +DV ++G+ NNS VSS Y WN
Sbjct: 162 NNPGWDPNDVGSHVGINNNSLVSSNYT--SWN 191
>At4g00320
Length = 773
Score = 26.9 bits (58), Expect = 3.9
Identities = 24/68 (35%), Positives = 33/68 (48%), Gaps = 8/68 (11%)
Query: 8 KSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILM 67
KS K ++ L+D IL DVP+ L ++TL LG R+T D S +L
Sbjct: 127 KSLYKCKSIVILKLKDEILVDVPRKVCLPSLKTLF---LG---RVTY--SDEDSLHRLLS 178
Query: 68 NTATLKDL 75
N L+DL
Sbjct: 179 NCPVLEDL 186
Score = 26.2 bits (56), Expect = 6.6
Identities = 27/119 (22%), Positives = 48/119 (39%), Gaps = 11/119 (9%)
Query: 24 PILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKDLKLAIKKKV 83
P+L D+ D + + + S R+T+ D I+MNT +LK LK+ +++
Sbjct: 181 PVLEDLVVERDRIDNLGKLSVVVKSLQRLTLKMSCPCHLDGIMMNTPSLKYLKVTDERQE 240
Query: 84 NYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDD---VLQNLGVRNNSQVSSTYLCH 139
+ + S S Y F++ L + D QN+ S + +CH
Sbjct: 241 SDSDNES--------DSDSPRYFYDFEDMPKLEEADFVLTFQNIKKDGISGLPDAMICH 291
>At5g64760 proteasome regulatory subunit-like
Length = 442
Score = 26.6 bits (57), Expect = 5.0
Identities = 14/44 (31%), Positives = 21/44 (46%)
Query: 70 ATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNK 113
ATL+D KL+ + + + I W S+W Y F+N K
Sbjct: 279 ATLEDKKLSEIPDFKMLLKQIVTMEVIQWTSLWNKYKDEFENEK 322
>At5g55860 myosin heavy chain-like
Length = 649
Score = 26.6 bits (57), Expect = 5.0
Identities = 24/73 (32%), Positives = 35/73 (47%), Gaps = 11/73 (15%)
Query: 6 EYKSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVI 65
E+KS K+A+V L E + A L VE + E T+ KL+TT ++
Sbjct: 484 EFKSLSKRAEVFDKLAEMKVAA------ALAQVEAVRASE-----NETLKKLETTQEEIK 532
Query: 66 LMNTATLKDLKLA 78
+ TAT + LK A
Sbjct: 533 KLKTATEEALKKA 545
>At5g13680 putative protein
Length = 1319
Score = 26.6 bits (57), Expect = 5.0
Identities = 21/64 (32%), Positives = 32/64 (49%), Gaps = 4/64 (6%)
Query: 9 SGLKKAKVLKALLEDPILADVPKNPTLED---VETLIGLELGSAMRITVLKLDTTSFDVI 65
SG K + + LLE P+LA + NP+ D VE G LG A R +++ ++ V
Sbjct: 510 SGFKASITFQTLLESPVLA-LAWNPSKRDSAFVEFEGGKVLGYASRSEIMETRSSDDSVC 568
Query: 66 LMNT 69
+T
Sbjct: 569 FPST 572
>At4g20010 unknown protein
Length = 371
Score = 26.6 bits (57), Expect = 5.0
Identities = 13/47 (27%), Positives = 23/47 (48%)
Query: 30 PKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKDLK 76
P++P + ++ L L A + KL+ FDV++ +K LK
Sbjct: 261 PRHPDFKSKDSSFSLWLNKAPNWVLPKLEGLEFDVLVPKARVVKQLK 307
>At3g19720 hypothetical protein
Length = 649
Score = 26.6 bits (57), Expect = 5.0
Identities = 17/39 (43%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Query: 36 EDVETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKD 74
E V +I L LG R TV KLDT S ++ ++ A LK+
Sbjct: 212 ESVPLIIPL-LGKEYRSTVRKLDTVSKELSSLDEAKLKE 249
>At1g43880 hypothetical protein
Length = 409
Score = 26.6 bits (57), Expect = 5.0
Identities = 32/103 (31%), Positives = 46/103 (44%), Gaps = 15/103 (14%)
Query: 1 MGEGAEYKSGLKK-------AKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRIT 53
M E A Y + K L AL E L+ VP LE+ +TLI EL S+++
Sbjct: 129 MAEAAAYADMMAKEGHAIAATNKLVALYEQR-LSQVPSANELEEGKTLIR-ELTSSVKAG 186
Query: 54 VLKLDTTSFDV----ILMNTATLKDLKLAIKKKVNYMEQSSMG 92
+ SF V + M +T KDL+ +K+ +ME G
Sbjct: 187 QDR--EVSFQVEIERLKMELSTSKDLEKGYDEKIGFMEMEIGG 227
>At4g01920 putative CHP-rich zinc finger protein
Length = 658
Score = 26.2 bits (56), Expect = 6.6
Identities = 10/25 (40%), Positives = 19/25 (76%)
Query: 45 ELGSAMRITVLKLDTTSFDVILMNT 69
+L S MR + +K++ TS++V+L N+
Sbjct: 592 DLSSLMRRSTIKIEGTSYEVVLNNS 616
>At1g53700 auxin-induced protein kinase like protein
Length = 476
Score = 26.2 bits (56), Expect = 6.6
Identities = 17/57 (29%), Positives = 28/57 (48%), Gaps = 5/57 (8%)
Query: 89 SSMGHRHIS--WRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQVSSTYLCHCWNC 143
+++ HR W S+ A LS D L ++++LG N +V +LCH +C
Sbjct: 62 TTLHHRRYDPHWTSIRAATTLSSDGRLHLRHFKLVRHLGTGNLGRV---FLCHLRDC 115
>At5g04230 phenylalanine ammonia-lyase PAL3
Length = 694
Score = 25.8 bits (55), Expect = 8.6
Identities = 25/92 (27%), Positives = 38/92 (41%), Gaps = 9/92 (9%)
Query: 2 GEGAEYKSGLKKAKVLKA-------LLEDPILADVPKNPTLEDVETLIGL--ELGSAMRI 52
G GLK A+V A L +P+ V +GL +A +
Sbjct: 434 GRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLISSRTTAEAV 493
Query: 53 TVLKLDTTSFDVILMNTATLKDLKLAIKKKVN 84
+LKL +T++ V L L+ L+ +KK VN
Sbjct: 494 VILKLMSTTYLVALCQAFDLRHLEEILKKAVN 525
>At4g33170 putative protein
Length = 990
Score = 25.8 bits (55), Expect = 8.6
Identities = 22/102 (21%), Positives = 42/102 (40%), Gaps = 10/102 (9%)
Query: 43 GLELGSAMRITVLKLDTTSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRH----ISW 98
GL L + + +K++ S + +TA + A + E + RH ++W
Sbjct: 432 GLSLSKQVHVHAIKINNVSDSFV--STALID----AYSRNRCMKEAEILFERHNFDLVAW 485
Query: 99 RSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQVSSTYLCHC 140
++ A Y S D +K L ++ G R++ +T C
Sbjct: 486 NAMMAGYTQSHDGHKTLKLFALMHKQGERSDDFTLATVFKTC 527
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.133 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,221,866
Number of Sequences: 26719
Number of extensions: 121362
Number of successful extensions: 363
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 348
Number of HSP's gapped (non-prelim): 25
length of query: 146
length of database: 11,318,596
effective HSP length: 90
effective length of query: 56
effective length of database: 8,913,886
effective search space: 499177616
effective search space used: 499177616
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC144765.13