BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144731.10 + phase: 0 /pseudo
         (222 letters)

Database: ara_mips 
           26,719 sequences; 11,318,596 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

At5g13240 unknown protein                                             201  3e-52
At3g50500 protein kinase SPK-2                                         30  1.3
At4g17895 ubiquitin-specific protease 20 (UBP20)                       29  1.7
At1g24100 putative indole-3-acetate beta-glucosyltransferase           28  5.0
At1g52430                                                              27  8.5

>At5g13240 unknown protein
          Length = 283

 Score =  201 bits (510), Expect = 3e-52
 Identities = 120/235 (51%), Positives = 147/235 (62%), Gaps = 25/235 (10%)

Query: 1   MKFLECAPLDRLSDFLSNLNLGERTIKGCIEAYSCKHSGADKKLSISLSNEILDYLGKSS 60
           MKFLE   LDRL+ FL +LNLGERTIKGC+EAYSCKH+G+DK+LS+SL NE+LDYLGKSS
Sbjct: 60  MKFLEYTNLDRLNVFLGHLNLGERTIKGCLEAYSCKHAGSDKRLSLSLENEMLDYLGKSS 119

Query: 61  DNDSPSPVDSLSSPVDSLSARTSPAGRHWFT*FLPSITCIQIM----ISAQLKLTSFSQR 116
           D DS       SSPVD L +R+S     +       +T  Q+      SA      FS+ 
Sbjct: 120 DTDS-------SSPVDLLLSRSSRKALIYLV-----LTLYQMYPDYDFSAVKAHQFFSEE 167

Query: 117 NHGT---VLSRFLKPTCL------KPQSLLDALFKAVDEVVSLDDCEIYGYLPDSEADPL 167
           +  T   + + ++           +  SLL+ ++KA+DEVV L +CEIY Y P+  ADP 
Sbjct: 168 SWDTFKQIFNNYMFEASKEWTERNEDGSLLEVIYKALDEVVKLAECEIYVYNPNPNADPF 227

Query: 168 PERGAIWSFNFLFYNRKLKRIVTFRLSCFSNLIADGFSLDEICDEYDEEIFADMD 222
            E GAIWSF FLFYNRKLKR+  FR SC SNL  D F  D    E DEEIFADMD
Sbjct: 228 LEEGAIWSFCFLFYNRKLKRVAGFRFSCTSNLANDAFLTDSPPYEEDEEIFADMD 282


>At3g50500 protein kinase SPK-2
          Length = 362

 Score = 29.6 bits (65), Expect = 1.3
 Identities = 13/35 (37%), Positives = 19/35 (54%)

Query: 188 IVTFRLSCFSNLIADGFSLDEICDEYDEEIFADMD 222
           I T R  C  + +AD   LD+  D++D E   D+D
Sbjct: 319 IPTVRNRCLDDFMADNLDLDDDMDDFDSESEIDVD 353


>At4g17895 ubiquitin-specific protease 20 (UBP20)
          Length = 695

 Score = 29.3 bits (64), Expect = 1.7
 Identities = 17/55 (30%), Positives = 27/55 (48%), Gaps = 1/55 (1%)

Query: 22  GERTIKGCIEAYSCKHSGADKKLSISLSNEILDYLGKSSDNDSPSPVDSLSSPVD 76
           G++  K C  A  C      K+  +SLSN+ + +L   S +   SP+  L  P+D
Sbjct: 522 GDKASKPCDSAGVCNQHVKTKEDFVSLSNDDV-FLSAESSSGEESPMGELLDPLD 575


>At1g24100 putative indole-3-acetate beta-glucosyltransferase
          Length = 460

 Score = 27.7 bits (60), Expect = 5.0
 Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 7/47 (14%)

Query: 52  ILDYLGKSSDNDSPSPVDSLSSP-----VDSLSARTSPA--GRHWFT 91
           +   L K S+ D P P D  S+P     + SLS    P+  GRHW T
Sbjct: 140 VCSVLRKFSNGDFPLPADPNSAPFRIRGLPSLSYDELPSFVGRHWLT 186


>At1g52430 
          Length = 1136

 Score = 26.9 bits (58), Expect = 8.5
 Identities = 12/35 (34%), Positives = 22/35 (62%), Gaps = 4/35 (11%)

Query: 3   FLECAPLDRLSDFLSNL----NLGERTIKGCIEAY 33
           FLEC  L+++ DFL+NL    N G + +   ++++
Sbjct: 396 FLECPELNQILDFLNNLKCERNDGAKLVSRAVDSF 430


  Database: ara_mips
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,978,382
  Number of sequences in database:  6832
  
  Database: /data/blast2/ara_mips_chr2
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,737,135
  Number of sequences in database:  4184
  
  Database: /data/blast2/ara_mips_chr3
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,236,886
  Number of sequences in database:  5377
  
  Database: /data/blast2/ara_mips_chr4
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,748,816
  Number of sequences in database:  4030
  
  Database: /data/blast2/ara_mips_chr5
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,569,679
  Number of sequences in database:  6098
  
  Database: /data/blast2/ara_mips_chl
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 25,951
  Number of sequences in database:  85
  
  Database: /data/blast2/ara_mips_mit
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 21,747
  Number of sequences in database:  113
  
Lambda     K      H
   0.323    0.139    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,135,096
Number of Sequences: 26719
Number of extensions: 216102
Number of successful extensions: 591
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 588
Number of HSP's gapped (non-prelim): 6
length of query: 222
length of database: 11,318,596
effective HSP length: 96
effective length of query: 126
effective length of database: 8,753,572
effective search space: 1102950072
effective search space used: 1102950072
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)