BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144725.4 + phase: 0
(126 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g40740 putative WRKY-type DNA binding protein 91 2e-19
At4g23810 unknown protein 74 1e-14
At5g22570 WRKY transcription factor 38 (WRKY38) 74 2e-14
At4g11070 WRKY transcription factor 41 (WRKY41) 74 3e-14
At3g56400 putative DNA-binding protein 72 1e-13
At1g66560 putative protein 71 1e-13
At2g46400 putative WRKY-type DNA binding protein 70 3e-13
At1g66550 WRKY transcription factor 67 (WRKY67) 70 3e-13
At1g66600 putative protein 69 6e-13
At5g24110 unknown protein 68 1e-12
At2g37260 putative WRKY-type DNA binding protein 68 1e-12
At2g40750 unknown protein 66 5e-12
At5g01900 WRKY DNA-binding protein 62 (WRKY62) 65 1e-11
At3g01080 WRKY transcription factor 58 (WRKY58) 61 1e-10
At1g29280 WRKY transcription factor 65 (WRKY65) 61 1e-10
At5g49520 unknown protein 61 2e-10
At2g46130 WRKY transcription factor 43 splice variant one (WRKY43) 61 2e-10
At4g26440 putative protein 60 2e-10
At1g64000 WRKY DNA binding protein, putative 60 2e-10
At5g52830 unknown protein 60 4e-10
>At2g40740 putative WRKY-type DNA binding protein
Length = 292
Score = 90.9 bits (224), Expect = 2e-19
Identities = 47/106 (44%), Positives = 58/106 (54%), Gaps = 12/106 (11%)
Query: 1 MPPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGG 60
+PP+D TW KYG KEI G +PR+YYRC H KLY C AKK+VQ+L D P F V Y G
Sbjct: 170 LPPDDNHTWRKYGQKEILGSRFPRAYYRCTHQKLYNCPAKKQVQRLNDDPFTFRVTYRGS 229
Query: 61 HTCRMSLTIPSLFVPARQPLDISIDGIQSTMPTSSTLYSRRISSGM 106
HTC S T P+ + ST+P SS + G+
Sbjct: 230 HTCYNS-TAPT-----------ASSATPSTIPISSVTTGHSVDYGL 263
>At4g23810 unknown protein
Length = 324
Score = 74.3 bits (181), Expect = 1e-14
Identities = 35/78 (44%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P +D F+W KYG K+I G +PRSYYRC H C A K+VQ+ +FEV Y G H
Sbjct: 156 PQDDVFSWRKYGQKDILGAKFPRSYYRCTHRSTQNCWATKQVQRSDGDATVFEVTYRGTH 215
Query: 62 TCRMSLT-IPSLFVPARQ 78
TC ++T P L P ++
Sbjct: 216 TCSQAITRTPPLASPEKR 233
>At5g22570 WRKY transcription factor 38 (WRKY38)
Length = 289
Score = 73.9 bits (180), Expect = 2e-14
Identities = 30/77 (38%), Positives = 47/77 (60%)
Query: 5 DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCR 64
DG+ W KYG K I + RSYYRC++ K + C A+K Q++ D+P ++ Y G HTC+
Sbjct: 111 DGYLWRKYGQKSIKKSNHQRSYYRCSYNKDHNCEARKHEQKIKDNPPVYRTTYFGHHTCK 170
Query: 65 MSLTIPSLFVPARQPLD 81
+ ++F+ + PLD
Sbjct: 171 TEHNLDAIFIAGQDPLD 187
>At4g11070 WRKY transcription factor 41 (WRKY41)
Length = 313
Score = 73.6 bits (179), Expect = 3e-14
Identities = 33/69 (47%), Positives = 41/69 (58%)
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P +D F+W KYG K+I G +PRSYYRC C A K+VQ+ P IFEV Y G H
Sbjct: 139 PHDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYCWATKQVQRSDGDPTIFEVTYRGTH 198
Query: 62 TCRMSLTIP 70
TC + +P
Sbjct: 199 TCSQGIPLP 207
>At3g56400 putative DNA-binding protein
Length = 294
Score = 71.6 bits (174), Expect = 1e-13
Identities = 30/67 (44%), Positives = 40/67 (58%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
ED F+W KYG KEI +PRSY+RC H C+A K+VQ++ P +F + Y G HTC
Sbjct: 120 EDAFSWRKYGQKEILNAKFPRSYFRCTHKYTQGCKATKQVQKVELEPKMFSITYIGNHTC 179
Query: 64 RMSLTIP 70
+ P
Sbjct: 180 NTNAETP 186
>At1g66560 putative protein
Length = 249
Score = 71.2 bits (173), Expect = 1e-13
Identities = 29/62 (46%), Positives = 38/62 (60%)
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
P+DGFTW KYG K I Y R YYRC + K C A+K+VQ + D+P ++ Y G H
Sbjct: 102 PDDGFTWRKYGQKTIKTSPYQRCYYRCTYAKDQNCNARKRVQMIQDNPPVYRTTYLGKHV 161
Query: 63 CR 64
C+
Sbjct: 162 CK 163
>At2g46400 putative WRKY-type DNA binding protein
Length = 295
Score = 70.1 bits (170), Expect = 3e-13
Identities = 33/67 (49%), Positives = 42/67 (62%), Gaps = 1/67 (1%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
+DG W KYG KEI G PR+YYRC H C A K+VQ+ P++FEV+Y G HTC
Sbjct: 104 DDGHCWRKYGQKEIHGSKNPRAYYRCTHRFTQDCLAVKQVQKSDTDPSLFEVKYLGNHTC 163
Query: 64 RMSLTIP 70
++T P
Sbjct: 164 N-NITSP 169
>At1g66550 WRKY transcription factor 67 (WRKY67)
Length = 254
Score = 70.1 bits (170), Expect = 3e-13
Identities = 29/64 (45%), Positives = 38/64 (59%)
Query: 1 MPPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGG 60
M P DGFTW KYG K I + R YYRC + K C A K+VQ++ D+P ++ Y G
Sbjct: 105 MCPNDGFTWRKYGQKTIKASAHKRCYYRCTYAKDQNCNATKRVQKIKDNPPVYRTTYLGK 164
Query: 61 HTCR 64
H C+
Sbjct: 165 HVCK 168
>At1g66600 putative protein
Length = 241
Score = 68.9 bits (167), Expect = 6e-13
Identities = 29/61 (47%), Positives = 37/61 (60%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
+DGFTW KYG K I +Y R YYRC + K C A K+VQ + D P ++ Y G HTC
Sbjct: 103 DDGFTWRKYGQKTIKTSLYQRCYYRCAYAKDQNCYATKRVQMIQDSPPVYRTTYLGQHTC 162
Query: 64 R 64
+
Sbjct: 163 K 163
>At5g24110 unknown protein
Length = 303
Score = 68.2 bits (165), Expect = 1e-12
Identities = 27/66 (40%), Positives = 41/66 (61%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
+DGF+W KYG K+I G +PR YYRC + K C A K+VQ+ ++ + E+ Y G H+C
Sbjct: 113 DDGFSWRKYGQKDILGAKFPRGYYRCTYRKSQGCEATKQVQRSDENQMLLEISYRGIHSC 172
Query: 64 RMSLTI 69
+ +
Sbjct: 173 SQAANV 178
>At2g37260 putative WRKY-type DNA binding protein
Length = 349
Score = 67.8 bits (164), Expect = 1e-12
Identities = 32/68 (47%), Positives = 38/68 (55%), Gaps = 3/68 (4%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
EDGF W KYG K + G YPRSYYRC CRA+K V++ D P F Y G H
Sbjct: 269 EDGFRWRKYGQKVVGGNAYPRSYYRCTSAN---CRARKHVERASDDPRAFITTYEGKHNH 325
Query: 64 RMSLTIPS 71
+ L+ PS
Sbjct: 326 HLLLSPPS 333
Score = 54.7 bits (130), Expect = 1e-08
Identities = 38/104 (36%), Positives = 53/104 (50%), Gaps = 16/104 (15%)
Query: 5 DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH--- 61
DG+ W KYG K++ G PRSYY+C H K C KKKV++ + E+ Y G H
Sbjct: 86 DGYNWRKYGQKQVKGSECPRSYYKCTHPK---CPVKKKVER-SVEGQVSEIVYQGEHNHS 141
Query: 62 --TC----RMSLTIPSLFVPARQPLDISIDGIQSTMPTSSTLYS 99
+C R S +I S F + P I+ +G P ++ LYS
Sbjct: 142 KPSCPLPRRASSSISSGF--QKPPKSIASEGSMGQDP-NNNLYS 182
>At2g40750 unknown protein
Length = 346
Score = 65.9 bits (159), Expect = 5e-12
Identities = 27/60 (45%), Positives = 36/60 (60%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
ED + W KYG KEI +PRSY+RC H C+A K+VQ+ +F++ Y G HTC
Sbjct: 152 EDRYAWRKYGQKEILNTTFPRSYFRCTHKPTQGCKATKQVQKQDQDSEMFQITYIGYHTC 211
>At5g01900 WRKY DNA-binding protein 62 (WRKY62)
Length = 263
Score = 64.7 bits (156), Expect = 1e-11
Identities = 27/61 (44%), Positives = 38/61 (62%)
Query: 5 DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCR 64
DGF W KYG K+I Y RSYY+C + K C AKK+VQ++ +P ++ Y G H C+
Sbjct: 111 DGFLWRKYGQKQIKESEYQRSYYKCAYTKDQNCEAKKQVQKIQHNPPLYSTTYFGQHICQ 170
Query: 65 M 65
+
Sbjct: 171 L 171
>At3g01080 WRKY transcription factor 58 (WRKY58)
Length = 423
Score = 61.2 bits (147), Expect = 1e-10
Identities = 29/60 (48%), Positives = 36/60 (59%), Gaps = 4/60 (6%)
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P +DG+ W KYG K I G YPRSYY+C HV C KKKV++ D I ++ Y G H
Sbjct: 165 PADDGYNWRKYGQKPIKGCEYPRSYYKCTHVN---CPVKKKVERSSD-GQITQIIYKGQH 220
Score = 44.7 bits (104), Expect = 1e-05
Identities = 20/58 (34%), Positives = 28/58 (47%), Gaps = 3/58 (5%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
+DG+ W KYG K + G +PRSYY+C C +K V++ Y G H
Sbjct: 306 DDGYRWRKYGQKVVKGNPHPRSYYKCTTPN---CTVRKHVERASTDAKAVITTYEGKH 360
>At1g29280 WRKY transcription factor 65 (WRKY65)
Length = 259
Score = 61.2 bits (147), Expect = 1e-10
Identities = 30/78 (38%), Positives = 40/78 (50%), Gaps = 2/78 (2%)
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
PP D + W KYG K I G YPR YYRC+ K C A+K+V++ D P + + Y H
Sbjct: 72 PPSDSWAWRKYGQKPIKGSPYPRGYYRCSSTK--GCPARKQVERSRDDPTMILITYTSEH 129
Query: 62 TCRMSLTIPSLFVPARQP 79
LT + P +P
Sbjct: 130 NHPWPLTSSTRNGPKPKP 147
>At5g49520 unknown protein
Length = 399
Score = 60.8 bits (146), Expect = 2e-10
Identities = 28/65 (43%), Positives = 36/65 (55%), Gaps = 3/65 (4%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
+DG+ W KYG K + YPRSYYRC V C KK+V++ D P+I Y G HT
Sbjct: 221 DDGYRWRKYGQKAVKNSPYPRSYYRCTTV---GCGVKKRVERSSDDPSIVMTTYEGQHTH 277
Query: 64 RMSLT 68
+T
Sbjct: 278 PFPMT 282
>At2g46130 WRKY transcription factor 43 splice variant one
(WRKY43)
Length = 97
Score = 60.8 bits (146), Expect = 2e-10
Identities = 26/58 (44%), Positives = 34/58 (57%), Gaps = 3/58 (5%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
+DG+ W KYG K + +YPRSYYRC + C KK+VQ+L +I E Y G H
Sbjct: 18 DDGYRWRKYGQKSVKNSLYPRSYYRCTQ---HMCNVKKQVQRLSKETSIVETTYEGIH 72
>At4g26440 putative protein
Length = 568
Score = 60.5 bits (145), Expect = 2e-10
Identities = 28/60 (46%), Positives = 36/60 (59%), Gaps = 4/60 (6%)
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P +DG+ W KYG K + G YPRSYY+C H C AKKKV++ +I E+ Y G H
Sbjct: 176 PADDGYNWRKYGQKLVKGSEYPRSYYKCTHPN---CEAKKKVER-SREGHIIEIIYTGDH 231
Score = 44.3 bits (103), Expect = 2e-05
Identities = 22/59 (37%), Positives = 28/59 (47%), Gaps = 3/59 (5%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
+DG+ W KYG K + G PRSYY+C C K V++ D Y G HT
Sbjct: 372 DDGYRWRKYGQKVVKGNPNPRSYYKCT---ANGCTVTKHVERASDDFKSVLTTYIGKHT 427
>At1g64000 WRKY DNA binding protein, putative
Length = 195
Score = 60.5 bits (145), Expect = 2e-10
Identities = 25/58 (43%), Positives = 34/58 (58%), Gaps = 3/58 (5%)
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
+DG+ W KYG K + +PRSYYRC + + C KK+VQ+L PN+ Y G H
Sbjct: 114 DDGYRWRKYGQKSVKNNAHPRSYYRCTY---HTCNVKKQVQRLAKDPNVVVTTYEGVH 168
>At5g52830 unknown protein
Length = 348
Score = 59.7 bits (143), Expect = 4e-10
Identities = 29/58 (50%), Positives = 36/58 (62%), Gaps = 2/58 (3%)
Query: 5 DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
D + W KYG K I G YPR+YYRC+ K C A+K+V++ PNIF V Y G HT
Sbjct: 166 DLWAWRKYGQKPIKGSPYPRNYYRCSSSK--GCLARKQVERSNLDPNIFIVTYTGEHT 221
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.139 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,012,353
Number of Sequences: 26719
Number of extensions: 114354
Number of successful extensions: 346
Number of sequences better than 10.0: 79
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 220
Number of HSP's gapped (non-prelim): 93
length of query: 126
length of database: 11,318,596
effective HSP length: 87
effective length of query: 39
effective length of database: 8,994,043
effective search space: 350767677
effective search space used: 350767677
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144725.4