BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144618.8 + phase: 0 /pseudo
(558 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12680 putative protein 493 e-140
At5g40640 unknown protein 280 2e-75
At3g27390 unknown protein 258 5e-69
At4g37030 hypothetical protein 224 1e-58
At3g42725 unknown protein 32 1.2
At4g30890 ubiquitin-specific protease 24 (UBP24) 31 2.1
At4g02690 putative glutamate-/aspartate-binding peptide 31 2.1
At2g25345 putative protein 31 2.1
At3g08030 unknown protein 30 2.7
At1g67120 hypothetical protein 30 3.5
At3g62120 multifunctional aminoacyl-tRNA ligase-like protein 29 6.0
At5g11720 alpha-glucosidase 1 29 7.9
>At4g12680 putative protein
Length = 590
Score = 493 bits (1270), Expect = e-140
Identities = 241/359 (67%), Positives = 292/359 (81%), Gaps = 3/359 (0%)
Query: 200 DVVLITSIAIWKSPYMLFRGWKRLLEDLVGRRGPFLETECVPFAGLAIILWPLAVLGAVL 259
DV+LIT++A++KSPYML +GWKRLLEDLVGR GPFLE+ CVPFAGLAI+LWPLAV GAV+
Sbjct: 211 DVLLITAVAVYKSPYMLLKGWKRLLEDLVGREGPFLESVCVPFAGLAILLWPLAVAGAVI 270
Query: 260 AAIIVSFFLSLYGGVVVHQEDSLKMGFAYIVSVVSLFDEYVNDLLYLREGSCLPRPIYRK 319
A+++ SFFL LY GV+VHQEDS +MG YI++ VSLFDEYVNDLLYLREG+ LPRP YR
Sbjct: 271 ASVLSSFFLGLYSGVIVHQEDSFRMGLNYIIAAVSLFDEYVNDLLYLREGTSLPRPCYRT 330
Query: 320 NVKHAVERKKLEGIDHN--LKNRRDSSQNSKHTLQQSRSMKWKIQQYKPVQVWDWFFKSC 377
+ V K++ G N LK++R SS SK +QSR++K I YKPVQVW+W FKSC
Sbjct: 331 KTE-TVHGKRILGESKNVDLKSKRSSSLGSKLVSEQSRTLKKAITLYKPVQVWEWLFKSC 389
Query: 378 EVNGRIVLRDGLISVKEIEECIFKGNCKKLSIKLPAWSLLQCLLTSAKSNSDGLVISDDI 437
EVNGRI+LRDGLI VK++EEC+ KGN KKL IKLPAW++LQCLL SAKSNS GLVI+D +
Sbjct: 390 EVNGRILLRDGLIDVKDVEECLVKGNSKKLYIKLPAWTVLQCLLASAKSNSSGLVITDGV 449
Query: 438 ELTRMNGPKDRVFEWFIGPLLIMKEQLKNLELEESEETCLKELVMRSKNDIPEDWDSTGF 497
ELT +N P+D+VF W +GPLLIMKEQ+KNL+L E EE CL++LVM KN+ EDWD+TGF
Sbjct: 450 ELTELNSPRDKVFVWLVGPLLIMKEQIKNLKLTEDEEFCLRKLVMVCKNERTEDWDNTGF 509
Query: 498 PSKDNVRRAQLQAIIRRLQGIGSSMSRMPTFRRKFRNLVKILYIEALQASASAKEGNNI 556
PS D VR+AQLQAIIRRLQG+ +SMSR+PTFRR+F NLVK+LYIEAL+ AS I
Sbjct: 510 PSSDTVRKAQLQAIIRRLQGMVASMSRIPTFRRRFMNLVKVLYIEALEMGASGNRAGGI 568
Score = 65.5 bits (158), Expect = 8e-11
Identities = 29/48 (60%), Positives = 38/48 (78%), Gaps = 1/48 (2%)
Query: 25 LIGPIAFAIMVVGNSAVIIGLWTAHVFWTYYCVARLYFLSKFFVICFI 72
+IGPI+ AI++VGNS VIIGLW AH WTYYC+AR + KF ++CF+
Sbjct: 34 IIGPISSAIILVGNSCVIIGLWPAHFIWTYYCLARCIPI-KFEILCFL 80
>At5g40640 unknown protein
Length = 586
Score = 280 bits (715), Expect = 2e-75
Identities = 157/347 (45%), Positives = 210/347 (60%), Gaps = 20/347 (5%)
Query: 200 DVVLITSIAIWKSPYMLFRGWKRLLEDLVGRRGPFLETECVPFAGLAIILWPLAVLGAVL 259
D +I+ +A+ KSPYMLF+GW RL DL+GR GPFLET CVP AGL I+LWPLAV+GAVL
Sbjct: 195 DFPVISLLALCKSPYMLFKGWHRLFHDLIGREGPFLETMCVPIAGLVILLWPLAVVGAVL 254
Query: 260 AAIIVSFFLSLYGGVVVHQEDSLKMGFAYIVSVVSLFDEYVNDLLYLREGSCLPRPIYRK 319
+++ S FL YGGVV +QE S G Y+V+ VS++DEY ND+L + EGSC PRPIYR+
Sbjct: 255 GSVVSSVFLGAYGGVVSYQESSFFFGLCYVVASVSIYDEYSNDVLDMPEGSCFPRPIYRR 314
Query: 320 NVKHAVERKKLEGIDHNLKNRRDSSQNSKHTLQQSRSMKWKIQQYKPVQVWDWFFKSCEV 379
N EG + K T + S K + KP+ + + F C
Sbjct: 315 NE---------EGASTAFSGGLSRPNSFKTTPSRGGSNKGPMIDLKPLDLLEALFVECRR 365
Query: 380 NGRIVLRDGLISVKEIEECIFKGNCKKLSIKLPAWSLLQCLLTSAKSNSDGLVISDDI-E 438
+G I++ G+I+ K+IEE + +S LPA+SLL LL S KSNS GL++ D + E
Sbjct: 366 HGEIMVTKGIINSKDIEEAKSSKGSQVISFGLPAYSLLHELLRSIKSNSTGLLLGDGVTE 425
Query: 439 LTRMNGPKDRVFEWFIGPLLIMKEQLKNLELEESEETCLKELVM------RSKNDIPEDW 492
+T N PKD F+WF+ P LI+K+Q++ L E EE L +LV+ R K+ I E
Sbjct: 426 ITTRNRPKDAFFDWFLNPFLILKDQIEAANLSEEEEEYLGKLVLLFGDSERLKSSIVE-- 483
Query: 493 DSTGFPSKDNVRRAQLQAIIRRLQGIGSSMSRMPTFRRKFRNLVKIL 539
+ P +R+A+L A RRLQG+ S+SR PTFRR F LVK L
Sbjct: 484 --SESPPLTELRKAELDAFARRLQGLTKSVSRYPTFRRHFVELVKKL 528
Score = 38.9 bits (89), Expect = 0.008
Identities = 18/53 (33%), Positives = 28/53 (51%), Gaps = 1/53 (1%)
Query: 21 LAGALIGPIAFAIMVVGNSAVIIGLWTAHVFWTYYCVARLYFLSKFFVICFIC 73
+ G ++ P+ + +GNSA+I+GL H WT Y +A L I F+C
Sbjct: 30 IKGIVLCPLICLTVAIGNSAIILGLLPVHAIWTLYSIASAKQLGPILKI-FLC 81
>At3g27390 unknown protein
Length = 599
Score = 258 bits (660), Expect = 5e-69
Identities = 159/374 (42%), Positives = 216/374 (57%), Gaps = 36/374 (9%)
Query: 200 DVVLITSIAIWKSPYMLFRGWKRLLEDLVGRRGPFLETECVPFAGLAIILWPLAVLGAVL 259
D +I+ +AI KSPYMLF+GW RL DL+GR GPFLET CVP AGLAI+LWPLAV GAV+
Sbjct: 195 DPPVISLVAICKSPYMLFKGWHRLFHDLIGREGPFLETMCVPIAGLAILLWPLAVTGAVI 254
Query: 260 AAIIVSFFLSLYGGVVVHQEDSLKMGFAYIVSVVSLFDEYVNDLLYLREGSCLPRPIYRK 319
++I S FL Y GVV +QE S G YIV+ VS++DEY D+L L EGSC PRP YR+
Sbjct: 255 GSVISSIFLGAYAGVVSYQESSFYYGLCYIVASVSIYDEYSTDILDLPEGSCFPRPKYRR 314
Query: 320 NVKHAVERKKLEGIDHNLKNRRDSSQNSKHTLQQSRSMKWKIQQYKPVQV---------- 369
+ E G L + +++S + S++ + KP+ V
Sbjct: 315 KDE---EPTPFSGPVPRLGSVKNASS------MRGGSVRVPMIDIKPLDVSPHLIFTKPN 365
Query: 370 -WDWFFKSCEVNGRIVLRDGLISVKEIEECIFKGNCKKLSIKLPAWSLLQCLLTSAKSNS 428
+ F C G ++ GLI+ K+IEE + +S+ LPA+ LL +L S K+NS
Sbjct: 366 LLNELFVECRRYGEVLATKGLINSKDIEEARSSKGSQVISVGLPAYGLLYEILRSVKANS 425
Query: 429 DGLVISDDI-ELTRMNGPKDRVFEWFIGPLLIMKEQLKNLELEESEETCLKELV------ 481
GL++SD + E+T MN PKD F+WF+ P LI+KEQ+K L E EE L LV
Sbjct: 426 SGLLLSDGVTEITTMNRPKDVFFDWFLNPFLILKEQMKATNLSEEEEEYLGRLVLLFGDP 485
Query: 482 --MRSKNDIPEDWDSTGFPSKDNVRRAQLQAIIRRLQGIGSSMSRMPTFRRKFRNLVKIL 539
++S N I + P +RA+L A RR+QG+ ++SR PTFRR F LVK L
Sbjct: 486 ERLKSSNAI------SASPPLTERKRAELDAFARRMQGLTKTVSRYPTFRRHFVALVKKL 539
Query: 540 YIEA-LQASASAKE 552
+ L+ + SAK+
Sbjct: 540 SEDLDLKDNNSAKD 553
Score = 42.4 bits (98), Expect = 7e-04
Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 1/65 (1%)
Query: 21 LAGALIGPIAFAIMVVGNSAVIIGLWTAHVFWTYYCVARLYFLSKFFVICFICSNSISLI 80
+ G ++ P+ ++ +GNSAVI+ L H+ WT+Y + + I F+C + I
Sbjct: 30 IKGIVLCPLVCLVVTIGNSAVILSLLPVHIVWTFYSIVSAKQVGPILKI-FLCLCLPAAI 88
Query: 81 NCWPL 85
WP+
Sbjct: 89 ILWPI 93
>At4g37030 hypothetical protein
Length = 519
Score = 224 bits (570), Expect = 1e-58
Identities = 121/327 (37%), Positives = 197/327 (60%), Gaps = 15/327 (4%)
Query: 200 DVVLITSIAIWKSPYMLFRGWKRLLEDLVGRRGPFLETECVPFAGLAIILWPLAVLGAVL 259
D+ L T+IA+ KSPY+L +GW RL +D + R GPFLE C+P AGL ++LWP+ V+G +L
Sbjct: 196 DIPLFTAIAVIKSPYLLLKGWYRLAQDAINREGPFLEIACIPVAGLTVLLWPIVVIGFIL 255
Query: 260 AAIIVSFFLSLYGGVVVHQEDSLKMGFAYIVSVVSLFDEYVNDLLYLREGSCLPRPIYRK 319
I S F+ LYG VVV QE S + G +Y+++VV FDEY ND LYLREG+ P+P YR
Sbjct: 256 VTIFSSIFVGLYGAVVVFQERSFRRGVSYVIAVVGEFDEYTNDWLYLREGTIFPKPRYR- 314
Query: 320 NVKHAVERKKLEGIDHNLKNRRDSSQNSKH-------TLQQSRSMKWKIQQYKPVQVWDW 372
+ ++ I H R +S S +L S S++ IQ+ + VQ+W+
Sbjct: 315 -MGRGSFSSEVSVIVHPSDVTRVNSSGSVDAPAMLVPSLVHSVSVREAIQEVRMVQIWEH 373
Query: 373 FFKSCEVNGRIVLRDGLISVKEIEECIFKG----NCKKLSIKLPAWSLLQCLLTSAKSNS 428
E+ G+ +L +++ ++ E + KG +++ LP+++LL LL+S K+
Sbjct: 374 MMGWFEMQGKELLDAEVLTPTDLYESL-KGRHGNESSIINVGLPSYALLHTLLSSIKAGV 432
Query: 429 DGLVISDDIELTRMNGPKDRVFEWFIGPLLIMKEQLKNLELEESEETCLKELVMRSKND- 487
G+++ D E+T +N P+D+ +W P++++K+Q++ L+L ESE L+++V+ ++
Sbjct: 433 HGVLLLDGSEVTHLNRPQDKFLDWVFNPIMVLKDQIRALKLGESEVKYLEKVVLFGNHEQ 492
Query: 488 IPEDWDSTGFPSKDNVRRAQLQAIIRR 514
E WD+ P ++N+R AQ+Q I RR
Sbjct: 493 RMEAWDNHSNPPQENLRTAQIQGISRR 519
Score = 44.3 bits (103), Expect = 2e-04
Identities = 19/39 (48%), Positives = 27/39 (68%)
Query: 21 LAGALIGPIAFAIMVVGNSAVIIGLWTAHVFWTYYCVAR 59
+ G ++GPIA ++VGN VI+ L+ AHV WT Y VA+
Sbjct: 30 IKGLIVGPIAGLTLIVGNVGVILCLFPAHVTWTIYAVAK 68
>At3g42725 unknown protein
Length = 202
Score = 31.6 bits (70), Expect = 1.2
Identities = 21/74 (28%), Positives = 37/74 (49%), Gaps = 9/74 (12%)
Query: 215 MLFRGWKRLLEDLVGRRGP-----FLETECVPFAGLAIILWPLAVLGAVLAAIIVSFFLS 269
MLF K+ L L+ R P + EC G+ + + PL ++ VLA ++ +++
Sbjct: 92 MLFSREKQSLSLLISTRDPREAKALVRVEC----GVFVAMIPLQIMVLVLACLLHCYWVR 147
Query: 270 LYGGVVVHQEDSLK 283
+ G+ V EDS +
Sbjct: 148 EFEGLDVESEDSFR 161
>At4g30890 ubiquitin-specific protease 24 (UBP24)
Length = 551
Score = 30.8 bits (68), Expect = 2.1
Identities = 15/48 (31%), Positives = 26/48 (53%)
Query: 460 MKEQLKNLELEESEETCLKELVMRSKNDIPEDWDSTGFPSKDNVRRAQ 507
M ++L L+ + + T K V+ S ND ++W++ G +K V R Q
Sbjct: 312 MHDELLKLKEQSPKVTASKSSVISSANDDGDEWETVGPKNKSAVTRTQ 359
>At4g02690 putative glutamate-/aspartate-binding peptide
Length = 248
Score = 30.8 bits (68), Expect = 2.1
Identities = 25/80 (31%), Positives = 40/80 (49%), Gaps = 8/80 (10%)
Query: 232 GPFL--ETECVPFAGLAIILWPLAVLGAVLAAIIVSFFLSLYGGVVVHQEDSLKMGFAY- 288
GPFL + F L IL+PL + ++ +VS ++ G +V+ D+L Y
Sbjct: 163 GPFLFGALTVLIFFALIQILFPLGRVSVMIYGCLVSI---IFCGYIVYDTDNLIKRHTYD 219
Query: 289 --IVSVVSLFDEYVNDLLYL 306
I + VSL+ + +N LYL
Sbjct: 220 EYIWAAVSLYLDIINLFLYL 239
>At2g25345 putative protein
Length = 600
Score = 30.8 bits (68), Expect = 2.1
Identities = 26/115 (22%), Positives = 53/115 (45%), Gaps = 4/115 (3%)
Query: 441 RMNGPKDRVFEWFIGPLLIMKEQLKNLELEESEETCLKELVMRSKNDIPEDWDSTGFPSK 500
+M+ +R+ LL K +++L ++ET +KE + R +D+ + ++T +K
Sbjct: 333 KMHSKLNRILLTMNERLLNSKTDMEDLIARLNQETAVKEYLNRKVDDLEVELETTKQRNK 392
Query: 501 DNVRRAQLQAIIRRLQGIGSSMSRMPTFRRKFRNLVKILYIEALQASASAKEGNN 555
+N+ QA++ Q + M R+K + L + +S S GN+
Sbjct: 393 ENLE----QALMTERQSVTKMQWDMEELRQKTFEMELKLKSKEDGSSDSKTSGNS 443
>At3g08030 unknown protein
Length = 365
Score = 30.4 bits (67), Expect = 2.7
Identities = 16/35 (45%), Positives = 25/35 (70%), Gaps = 2/35 (5%)
Query: 464 LKNLELEES-EETCLKELVMRSKNDIPEDWDSTGF 497
L+N EES ++T +K+ V+ KN +PE W++TGF
Sbjct: 29 LRNGNFEESPKKTDMKKTVLLGKNALPE-WETTGF 62
>At1g67120 hypothetical protein
Length = 5138
Score = 30.0 bits (66), Expect = 3.5
Identities = 28/113 (24%), Positives = 54/113 (47%), Gaps = 7/113 (6%)
Query: 248 ILWPLAVLGAVLAAIIVSFFLSLYGG---VVVHQEDSLKMGFAYIVSVVSLFDEYVNDLL 304
+LW + +G ++A ++ SFF ++ G + H + S + F Y S + ++
Sbjct: 2827 VLWKNSQIGLMIAHLVTSFFHLMFTGPTEYLRHWQLSDSVTF-YADSACENSYRCSDSII 2885
Query: 305 YLREGSCLPRP-IYRKNVKHAVERK--KLEGIDHNLKNRRDSSQNSKHTLQQS 354
Y + S + K+V HA+E+K K++GI + + SS N ++ S
Sbjct: 2886 YTHQKSFESETFVAIKSVFHAIEKKQNKMDGIQNLISLIGSSSHNKLKSVTHS 2938
>At3g62120 multifunctional aminoacyl-tRNA ligase-like protein
Length = 530
Score = 29.3 bits (64), Expect = 6.0
Identities = 21/100 (21%), Positives = 43/100 (43%), Gaps = 10/100 (10%)
Query: 319 KNVKHAVERKKLEGIDHNLKNRRDSSQNSKHTLQQSRSMKWKIQQYKPVQVWDWFFKSC- 377
K VK E+ K E + + SS K +++ + ++ K +W+ + C
Sbjct: 10 KVVKEKKEKVKKEKVVKEKVAKASSSGQKKKDVKKETGLGLSVK--KDENFGEWYSEVCK 67
Query: 378 -------EVNGRIVLRDGLISVKEIEECIFKGNCKKLSIK 410
+++G +LR +++ EI + F KK+ +K
Sbjct: 68 QDMIEYYDISGCYILRPWSMAIWEIMQIFFDAEIKKMKVK 107
>At5g11720 alpha-glucosidase 1
Length = 902
Score = 28.9 bits (63), Expect = 7.9
Identities = 21/102 (20%), Positives = 44/102 (42%), Gaps = 13/102 (12%)
Query: 262 IIVSFFLSLYGGVVVHQEDSLKMGFAYIVSVVSLFDEYVNDLLYLREGSCLPRPIYRKNV 321
++V FF VV+ +E+S +G+ Y+V V + + +L + P +Y ++
Sbjct: 13 VVVVFFSLRSSQVVLEEEESTVVGYGYVVRSVGV--DSNRQVLTAKLDLIKPSSVYAPDI 70
Query: 322 KHAVERKKLEGIDHNLKNRRDSSQNSKHTLQQSRSMKWKIQQ 363
K NL ++S+ + + S +W+I +
Sbjct: 71 KSL-----------NLHVSLETSERLRIRITDSSQQRWEIPE 101
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.333 0.144 0.458
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,635,455
Number of Sequences: 26719
Number of extensions: 478234
Number of successful extensions: 1467
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1444
Number of HSP's gapped (non-prelim): 19
length of query: 558
length of database: 11,318,596
effective HSP length: 104
effective length of query: 454
effective length of database: 8,539,820
effective search space: 3877078280
effective search space used: 3877078280
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 63 (28.9 bits)
Medicago: description of AC144618.8