BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144541.1 - phase: 0
(124 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g27770 60S ribosomal protein L22 - like 224 6e-60
At3g05560 putative 60S ribosomal protein L22 224 8e-60
At1g02830 60S ribosomal protein L22 171 1e-43
At5g10230 annexin -like protein 30 0.30
At3g25580 unknown protein 30 0.30
At4g15417 unknown protein 29 0.52
At3g20555 putative protein 29 0.52
At2g18990 putative ATP binding protein 29 0.68
At1g18410 kinesin-related protein, putative 28 0.88
At5g17710 chloroplast GrpE protein 28 1.2
At3g24560 RASBERRY3 (RSY3) like protein 28 1.5
At5g08170 unknown protein 27 2.6
At2g30960 hypothetical protein 27 2.6
At2g18700 putative trehalose-6-phosphate synthase 27 2.6
At1g65660 step II splicing factor, putative 27 2.6
At1g36675 putative protein 27 3.4
At1g70200 unknown protein 26 4.4
At1g64385 unknown protein 26 4.4
At3g57300 helicase-like protein 26 5.7
At3g28750 unknown protein 26 5.7
>At5g27770 60S ribosomal protein L22 - like
Length = 124
Score = 224 bits (572), Expect = 6e-60
Identities = 109/124 (87%), Positives = 119/124 (95%)
Query: 1 MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT 60
MSRG+AA AKGKKKG +FTIDC+KPV+DKIM+IASLEKFLQERIKVGGKAGALGDSVS+T
Sbjct: 1 MSRGNAAAAKGKKKGVSFTIDCSKPVDDKIMEIASLEKFLQERIKVGGKAGALGDSVSIT 60
Query: 61 REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG 120
REKSKITVT+D FSKRYLKYLTKKYLKKHNVRDWLRVIA+NKDR++YELRYFNIAENE
Sbjct: 61 REKSKITVTADGQFSKRYLKYLTKKYLKKHNVRDWLRVIAANKDRNLYELRYFNIAENEA 120
Query: 121 EEED 124
EEED
Sbjct: 121 EEED 124
>At3g05560 putative 60S ribosomal protein L22
Length = 124
Score = 224 bits (571), Expect = 8e-60
Identities = 109/124 (87%), Positives = 119/124 (95%)
Query: 1 MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT 60
MSRG AA AKGKKKG +FTIDC+KPV+DKIM+IASLEKFLQERIKVGGKAGALGDSV++T
Sbjct: 1 MSRGGAAVAKGKKKGVSFTIDCSKPVDDKIMEIASLEKFLQERIKVGGKAGALGDSVTIT 60
Query: 61 REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG 120
REKSKITVT+D FSKRYLKYLTKKYLKKHNVRDWLRVIA+NKDR++YELRYFNIAENEG
Sbjct: 61 REKSKITVTADGQFSKRYLKYLTKKYLKKHNVRDWLRVIAANKDRNLYELRYFNIAENEG 120
Query: 121 EEED 124
EEED
Sbjct: 121 EEED 124
>At1g02830 60S ribosomal protein L22
Length = 127
Score = 171 bits (432), Expect = 1e-43
Identities = 81/119 (68%), Positives = 101/119 (84%)
Query: 6 AAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREKSK 65
++GA KKKG +F IDC+KPV+D I++IA+LEKFLQERIKV GKAGALG+SVS+TR K
Sbjct: 8 SSGAGAKKKGVSFVIDCSKPVDDTILEIATLEKFLQERIKVRGKAGALGNSVSITRYNGK 67
Query: 66 ITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEGEEED 124
I V ++SNFSKRYLKYLTKKYLKK+N+RDWLRVIASNKD+++YE+RYF I + E+
Sbjct: 68 INVNANSNFSKRYLKYLTKKYLKKYNLRDWLRVIASNKDKNVYEVRYFRIDDEVASYEE 126
>At5g10230 annexin -like protein
Length = 316
Score = 30.0 bits (66), Expect = 0.30
Identities = 20/76 (26%), Positives = 37/76 (48%), Gaps = 2/76 (2%)
Query: 38 KFLQERIKVGGKAGALGDSVSVTREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLR 97
K L E+IK KA A D + + +SK +++ N K KYLK+ + ++++
Sbjct: 173 KILHEKIKE--KAYADDDLIRILTTRSKAQISATLNHYKNNFGTSMSKYLKEDSENEYIQ 230
Query: 98 VIASNKDRSIYELRYF 113
++ + Y +YF
Sbjct: 231 LLKAVIKCLTYPEKYF 246
>At3g25580 unknown protein
Length = 210
Score = 30.0 bits (66), Expect = 0.30
Identities = 24/82 (29%), Positives = 40/82 (48%), Gaps = 14/82 (17%)
Query: 23 AKPVEDKIMD-IASLEKFLQERIKVGGKAGALGDSVSVTREKSKITVTSDSNFSKRYLKY 81
AK +EDKI D IASLEK + D + V RE+ + + KR++
Sbjct: 17 AKAMEDKIDDEIASLEKLDE-------------DDLEVLRERRLKQMKKMAEKKKRWMSI 63
Query: 82 LTKKYLKKHNVRDWLRVIASNK 103
+Y + H+ +D+ V+ S++
Sbjct: 64 GHGEYSEIHSEKDFFSVVKSSE 85
>At4g15417 unknown protein
Length = 203
Score = 29.3 bits (64), Expect = 0.52
Identities = 28/115 (24%), Positives = 47/115 (40%), Gaps = 11/115 (9%)
Query: 1 MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT 60
+S GS K T TI A P D++M+I SLEK L + K D +
Sbjct: 24 VSMGSQLSNAIDGKDNTKTIGSADP--DQLMEIESLEKILNYKFK---------DKSLLL 72
Query: 61 REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNI 115
+ + + D + S L+ L L + D++++ + +LR N+
Sbjct: 73 KAFTDASYVDDKSESYELLELLGDSILNMGIIYDFIKLYPKEAPGPLTKLRAVNV 127
>At3g20555 putative protein
Length = 110
Score = 29.3 bits (64), Expect = 0.52
Identities = 17/31 (54%), Positives = 20/31 (63%), Gaps = 6/31 (19%)
Query: 41 QERIKVGGKAGALGDSVSVTREKSKITVTSD 71
+ RIKVGGK GAL ++KITVTSD
Sbjct: 46 KSRIKVGGKPGAL------CYNQNKITVTSD 70
>At2g18990 putative ATP binding protein
Length = 211
Score = 28.9 bits (63), Expect = 0.68
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 14/82 (17%)
Query: 23 AKPVEDKIMD-IASLEKFLQERIKVGGKAGALGDSVSVTREKSKITVTSDSNFSKRYLKY 81
AK +EDKI D IASLEK + D + V RE+ + + KR++
Sbjct: 17 AKAMEDKIDDEIASLEKLDE-------------DDLEVLRERRLKQMKKMAEKKKRWISL 63
Query: 82 LTKKYLKKHNVRDWLRVIASNK 103
+Y + H+ +D+ V+ +++
Sbjct: 64 GHGEYSEIHSEKDFFSVVKASE 85
>At1g18410 kinesin-related protein, putative
Length = 1081
Score = 28.5 bits (62), Expect = 0.88
Identities = 19/55 (34%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Query: 10 KGKKKGATFTI-DCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREK 63
+ K KGAT I D K +E D + K L+ER++ K G D+V + E+
Sbjct: 384 ESKTKGATAGIEDRVKELEQMRKDASVARKALEERVRELEKMGKEADAVKMNLEE 438
>At5g17710 chloroplast GrpE protein
Length = 326
Score = 28.1 bits (61), Expect = 1.2
Identities = 29/100 (29%), Positives = 51/100 (51%), Gaps = 8/100 (8%)
Query: 27 EDKIMDI-ASLEKFLQERIKVGGKAGALGDSVSVTREKSKITVTSD-SNFSKR-YLKYLT 83
E KI +I ASL+ E+ + K +L + +SV R++ I +++D NF KR + L
Sbjct: 128 EGKIAEIEASLKSIEDEKFLLADKVASLSNELSVERDR-LIRISADFDNFRKRTERERLN 186
Query: 84 KKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEGEEE 123
+ V + L + N +R+ +++ E EGEE+
Sbjct: 187 LVSNAQGEVVENLLAVLDNFERAKSQIK----VETEGEEK 222
>At3g24560 RASBERRY3 (RSY3) like protein
Length = 660
Score = 27.7 bits (60), Expect = 1.5
Identities = 20/73 (27%), Positives = 37/73 (50%), Gaps = 2/73 (2%)
Query: 6 AAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTR--EK 63
A G+KG K + ++DC P + ++++I+ E + ++ A + D V+ T E
Sbjct: 406 APGSKGTKIIVSCSVDCPLPSKTELLNISFNETPSDDLGQIIADAKSFSDHVAPTSLFEV 465
Query: 64 SKITVTSDSNFSK 76
+ V S+S SK
Sbjct: 466 QFLDVASESVLSK 478
>At5g08170 unknown protein
Length = 383
Score = 26.9 bits (58), Expect = 2.6
Identities = 15/62 (24%), Positives = 27/62 (43%)
Query: 26 VEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREKSKITVTSDSNFSKRYLKYLTKK 85
V KI+ + + +F I GG G+ + E+ + + + SK ++ KK
Sbjct: 142 VSRKILALERIPRFQHSMILEGGSIHVDGEGTCLVTEECLLNKNRNPHMSKEQIEEELKK 201
Query: 86 YL 87
YL
Sbjct: 202 YL 203
>At2g30960 hypothetical protein
Length = 260
Score = 26.9 bits (58), Expect = 2.6
Identities = 25/93 (26%), Positives = 45/93 (47%), Gaps = 10/93 (10%)
Query: 21 DCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREKSKITVTSDSNFSKRYLK 80
D K E+K M+ ++ + F +++ + G G++ V +EK ++TV +S +
Sbjct: 63 DLKKAEEEKRMNRSNRKHFGRKKESIDG-----GEARFVEKEKPEVTVAIESGGGEDAAD 117
Query: 81 YLTKKYLKKHNVRDWLRVIAS----NKDRSIYE 109
L KK KK N W + S N+D ++ E
Sbjct: 118 GLPKK-KKKKNKGAWRKRYNSKTNKNQDGAVVE 149
>At2g18700 putative trehalose-6-phosphate synthase
Length = 862
Score = 26.9 bits (58), Expect = 2.6
Identities = 17/61 (27%), Positives = 27/61 (43%), Gaps = 5/61 (8%)
Query: 58 SVTREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSI---YELRYFN 114
+VT S SD + R+ K+ KY+ HNV W R + R+ Y R++
Sbjct: 493 AVTNAMSSAMTMSDKEKNLRHQKH--HKYISSHNVAYWARSYDQDLQRACKDHYNKRFWG 550
Query: 115 I 115
+
Sbjct: 551 V 551
>At1g65660 step II splicing factor, putative
Length = 535
Score = 26.9 bits (58), Expect = 2.6
Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Query: 3 RGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTRE 62
+G A + G++ +D AK E + MD A +EK + R GG G + ++ + +
Sbjct: 187 KGDDANSDGEEDEDDLRVDEAKVDESRQMDFAKVEK--RVRTTGGGSTGTV-RNLRIRED 243
Query: 63 KSKITVTSDSN 73
+K + D N
Sbjct: 244 TAKYLLNLDVN 254
>At1g36675 putative protein
Length = 268
Score = 26.6 bits (57), Expect = 3.4
Identities = 12/42 (28%), Positives = 23/42 (54%)
Query: 37 EKFLQERIKVGGKAGALGDSVSVTREKSKITVTSDSNFSKRY 78
E +++ VG A +G ++ + K K +SD+ F+KR+
Sbjct: 146 ESDVRDANSVGSSARPIGSDIAKKKTKKKDLSSSDNYFTKRF 187
>At1g70200 unknown protein
Length = 538
Score = 26.2 bits (56), Expect = 4.4
Identities = 11/34 (32%), Positives = 21/34 (61%)
Query: 57 VSVTREKSKITVTSDSNFSKRYLKYLTKKYLKKH 90
+S + E S I +T DSN ++ K + K+ +++H
Sbjct: 341 ISSSIESSPIEMTRDSNTKLKFEKQVVKREIREH 374
>At1g64385 unknown protein
Length = 351
Score = 26.2 bits (56), Expect = 4.4
Identities = 20/68 (29%), Positives = 30/68 (43%), Gaps = 7/68 (10%)
Query: 57 VSVTREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIA 116
V V EK K+ + + + K +KK D V+AS+K R EL+ A
Sbjct: 166 VFVRLEKDKVQLLQNEDIK-------VKVSIKKGGSNDSAIVLASSKGRCRLELKDLAAA 218
Query: 117 ENEGEEED 124
+E E +D
Sbjct: 219 AHETESDD 226
>At3g57300 helicase-like protein
Length = 1507
Score = 25.8 bits (55), Expect = 5.7
Identities = 13/39 (33%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Query: 11 GKKKGAT-FTIDCAKPVEDKIMDIASLEKFLQERIKVGG 48
G+ K T + + C + VE+KI+ AS + +Q+ + GG
Sbjct: 1319 GQTKDVTVYRLICKETVEEKILHRASQKNTVQQLVMTGG 1357
>At3g28750 unknown protein
Length = 333
Score = 25.8 bits (55), Expect = 5.7
Identities = 23/89 (25%), Positives = 39/89 (42%), Gaps = 9/89 (10%)
Query: 4 GSAAGAKGKKKGATFTIDCAKPV---EDKIMDIASLEK-----FLQERIKVGGKAGALGD 55
G GA G+ GA F I V +K ++ + K +Q +KVGG+ A G
Sbjct: 217 GKFVGAHGQV-GAGFEIGAKAKVGVQSEKFLEYGPITKPKGKVGVQGEVKVGGQVHAAGK 275
Query: 56 SVSVTREKSKITVTSDSNFSKRYLKYLTK 84
+ + ++K+ + S + +KY K
Sbjct: 276 AKVRAQVEAKVGGETGSGYYDGIMKYFGK 304
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.312 0.131 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,615,728
Number of Sequences: 26719
Number of extensions: 97325
Number of successful extensions: 234
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 222
Number of HSP's gapped (non-prelim): 33
length of query: 124
length of database: 11,318,596
effective HSP length: 87
effective length of query: 37
effective length of database: 8,994,043
effective search space: 332779591
effective search space used: 332779591
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144541.1