BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144516.12 - phase: 0
(105 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g59835 unknown protein 114 8e-27
At3g59670 putative protein 27 1.3
At3g28770 hypothetical protein 27 1.3
At1g48490 IRE - like protein kinase 27 1.3
At2g01110 TATC-like protein 27 1.7
At1g64570 hypothetical protein 27 1.7
At2g41710 AP2 domain transcription factor like protein 27 2.2
At1g06740 mudrA-like protein 27 2.2
At5g64390 HEN4 26 2.9
At5g49340 putative protein 26 2.9
At2g25830 unknown protein 26 2.9
At1g72350 putative SRF-type transcription factor 26 2.9
At2g26860 unknown protein 26 3.8
At1g69310 putative WRKY transcription factor 26 3.8
At5g40200 putative serine protease (MSN9.10) 25 4.9
At1g33390 RNA helicase, putative 25 4.9
At1g08510 cyl-(acyl carrier protein) thioesterase like protein 25 4.9
At3g57920 squamosa promoter-binding protein homolog 25 6.4
At3g18690 unknown protein 25 6.4
At3g16180 putative transport protein 25 6.4
>At3g59835 unknown protein
Length = 97
Score = 114 bits (285), Expect = 8e-27
Identities = 61/105 (58%), Positives = 71/105 (67%), Gaps = 8/105 (7%)
Query: 1 MGLLWWRKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFE 60
MG+L W G +++ S E K T APGMNGA+EV RP A+V FE
Sbjct: 1 MGILSWFTGSPKPSQSETSKPEMI------QMPKSETPAPGMNGAIEVPRPDRATV--FE 52
Query: 61 FGSVAASNDKVTLAGYCPVSEDLEPCRWEILPAVESNAPQFRVVF 105
FGSVAA+ D+VTLAGYCPVS+DLEPCRWEILPA +APQFRVVF
Sbjct: 53 FGSVAATGDRVTLAGYCPVSDDLEPCRWEILPADGKDAPQFRVVF 97
>At3g59670 putative protein
Length = 510
Score = 27.3 bits (59), Expect = 1.3
Identities = 15/51 (29%), Positives = 25/51 (48%)
Query: 12 SQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFG 62
S+ A+ S++EN + +SA PT A G + NAS + ++G
Sbjct: 305 SKNTARFSSSENLSLLAASSAPSPTVSAGGNGDVISFGAIYNASQHMADYG 355
>At3g28770 hypothetical protein
Length = 2081
Score = 27.3 bits (59), Expect = 1.3
Identities = 14/43 (32%), Positives = 21/43 (48%)
Query: 10 KNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPK 52
KN + + + + AAKN G+S + EA G NG + K
Sbjct: 291 KNLESKEDVKSEVEAAKNDGSSMTENLGEAQGNNGVSTIDNEK 333
Score = 26.6 bits (57), Expect = 2.2
Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 5/65 (7%)
Query: 10 KNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASND 69
KN + + + + AAKN G+S EA NG N +++ GS ++ND
Sbjct: 349 KNLESKEDVKSEVEAAKNAGSSMTGKLEEAQRNNGV-----STNETMNSENKGSGESTND 403
Query: 70 KVTLA 74
K+ A
Sbjct: 404 KMVNA 408
>At1g48490 IRE - like protein kinase
Length = 1235
Score = 27.3 bits (59), Expect = 1.3
Identities = 23/72 (31%), Positives = 35/72 (47%), Gaps = 5/72 (6%)
Query: 8 KGKNSQPEAKLSTT-ENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAA 66
KGK + E S+ ++ + + G+ A KP TE P + +VK ASVS S+
Sbjct: 61 KGKEASLEVHSSSPGKSNSSSSGSEAKKPITETPATS---DVKEESPASVSPIMASSLGL 117
Query: 67 SNDKVTLAGYCP 78
+ K T +G P
Sbjct: 118 NRIK-TRSGPLP 128
>At2g01110 TATC-like protein
Length = 340
Score = 26.9 bits (58), Expect = 1.7
Identities = 24/61 (39%), Positives = 32/61 (52%), Gaps = 10/61 (16%)
Query: 11 NSQPEAKL--STTENAAKN-GGASAVK----PTTEAPGMNGAVEVKRP---KNASVSVFE 60
NS EA L S T+ +K G SA+ PT PG+ AVE + P ++ S SV+E
Sbjct: 37 NSWREAGLRYSVTQRRSKGFGPVSALNDDDSPTETTPGVGSAVEDRPPDSSEDRSSSVYE 96
Query: 61 F 61
F
Sbjct: 97 F 97
>At1g64570 hypothetical protein
Length = 1239
Score = 26.9 bits (58), Expect = 1.7
Identities = 19/78 (24%), Positives = 31/78 (39%), Gaps = 20/78 (25%)
Query: 9 GKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASN 68
GKN + LST+ + NGG+ + +EAP + +A N
Sbjct: 1006 GKNVNLDIYLSTSSSKVNNGGSVSAANISEAPDI--------------------CMAQLN 1045
Query: 69 DKVTLAGYCPVSEDLEPC 86
D + G P S+++ C
Sbjct: 1046 DGSEVPGSAPPSDNISRC 1063
>At2g41710 AP2 domain transcription factor like protein
Length = 423
Score = 26.6 bits (57), Expect = 2.2
Identities = 10/30 (33%), Positives = 15/30 (49%)
Query: 5 WWRKGKNSQPEAKLSTTENAAKNGGASAVK 34
WW KN QPE+ +E+A + +K
Sbjct: 228 WWGANKNRQPESSSKASEDANVEDAGTELK 257
>At1g06740 mudrA-like protein
Length = 726
Score = 26.6 bits (57), Expect = 2.2
Identities = 18/53 (33%), Positives = 27/53 (49%), Gaps = 7/53 (13%)
Query: 52 KNASVSV-FEFGSVAASNDKVTLAGYCPVSEDLEPCRWEILPAVESNAPQFRV 103
KNA++S+ FE ++ +DK C + + C W I A SNAP F +
Sbjct: 173 KNAAISLRFEMRTI--KSDKTRFTAKC----NSKGCPWRIHCAKVSNAPTFTI 219
>At5g64390 HEN4
Length = 857
Score = 26.2 bits (56), Expect = 2.9
Identities = 18/60 (30%), Positives = 26/60 (43%), Gaps = 7/60 (11%)
Query: 12 SQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASNDKV 71
+Q ++K A NG A K E VEV + + A + VFE + A +D V
Sbjct: 98 AQADSKSRVKLGANNNGNAEGEKKEEE-------VEVSKAQGALIKVFELLAAEADSDTV 150
>At5g49340 putative protein
Length = 457
Score = 26.2 bits (56), Expect = 2.9
Identities = 13/33 (39%), Positives = 17/33 (51%)
Query: 54 ASVSVFEFGSVAASNDKVTLAGYCPVSEDLEPC 86
AS +F+ V ++ V L GYCP ED C
Sbjct: 98 ASCDIFDGTWVFDDSEPVYLPGYCPFVEDKFNC 130
>At2g25830 unknown protein
Length = 331
Score = 26.2 bits (56), Expect = 2.9
Identities = 18/52 (34%), Positives = 25/52 (47%), Gaps = 5/52 (9%)
Query: 7 RKGKNSQPEAKL-----STTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKN 53
RKG +AKL +A K GG + V TT A ++ A E+ PK+
Sbjct: 94 RKGAQDSKKAKLYCRIGKEVVSAVKKGGPNPVSNTTLATILDKAKELDVPKD 145
>At1g72350 putative SRF-type transcription factor
Length = 224
Score = 26.2 bits (56), Expect = 2.9
Identities = 9/23 (39%), Positives = 13/23 (56%)
Query: 2 GLLWWRKGKNSQPEAKLSTTENA 24
GL+WW + S PE + +NA
Sbjct: 133 GLMWWERAVESVPEEHMEEYKNA 155
>At2g26860 unknown protein
Length = 405
Score = 25.8 bits (55), Expect = 3.8
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 2/30 (6%)
Query: 56 VSVFEFGSVAASNDKVT--LAGYCPVSEDL 83
+ E SVA SN++ L YCPV EDL
Sbjct: 157 LKTLELDSVAYSNEESLRLLLSYCPVLEDL 186
>At1g69310 putative WRKY transcription factor
Length = 287
Score = 25.8 bits (55), Expect = 3.8
Identities = 14/31 (45%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Query: 11 NSQPEAKLSTTENAAKNGGASAVK-PTTEAP 40
N+ P A S++E+ A+N ASA K P E P
Sbjct: 89 NNNPSATSSSSEDPAENSTASAEKTPPPETP 119
>At5g40200 putative serine protease (MSN9.10)
Length = 592
Score = 25.4 bits (54), Expect = 4.9
Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Query: 56 VSVFEFGSVAASNDKVTLAGYCPVSED 82
VS EFG + A D VT+ GY P+ D
Sbjct: 215 VSPVEFGDLPALQDAVTVVGY-PIGGD 240
>At1g33390 RNA helicase, putative
Length = 1237
Score = 25.4 bits (54), Expect = 4.9
Identities = 23/79 (29%), Positives = 32/79 (40%), Gaps = 7/79 (8%)
Query: 27 NGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASNDKVTLAGYCPVSEDLEPC 86
+G AS+ + + A KN SVS S+AA N + V P
Sbjct: 591 DGFASSFVEEGKLDALRAAFNALADKNGSVSAEPAKSIAAENQEAE-----QVKNKFSPG 645
Query: 87 RWEILP--AVESNAPQFRV 103
+ +LP A+ S A Q RV
Sbjct: 646 KLRVLPLYAMLSPAAQLRV 664
>At1g08510 cyl-(acyl carrier protein) thioesterase like protein
Length = 412
Score = 25.4 bits (54), Expect = 4.9
Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 8/36 (22%)
Query: 23 NAAKNGGASAVKPTTEAP--------GMNGAVEVKR 50
N+A N G VKP +AP G+ G+V++ R
Sbjct: 36 NSAPNSGRMKVKPNAQAPPKINGKKVGLPGSVDIVR 71
>At3g57920 squamosa promoter-binding protein homolog
Length = 354
Score = 25.0 bits (53), Expect = 6.4
Identities = 16/61 (26%), Positives = 28/61 (45%), Gaps = 9/61 (14%)
Query: 47 EVKRPKNASVSVFEFGSVAASNDKVTLAGYCPVSEDLEPC---------RWEILPAVESN 97
+++ P N F S+ + +DKVT+A P+S P WE++ +SN
Sbjct: 236 QLQTPTNTWRPSSGFDSMISFSDKVTMAQPPPISTHQPPISTHQQYLSQTWEVIAGEKSN 295
Query: 98 A 98
+
Sbjct: 296 S 296
>At3g18690 unknown protein
Length = 222
Score = 25.0 bits (53), Expect = 6.4
Identities = 10/21 (47%), Positives = 15/21 (70%)
Query: 9 GKNSQPEAKLSTTENAAKNGG 29
G + P A+L++TENA+ GG
Sbjct: 104 GGDVSPAARLASTENASPRGG 124
>At3g16180 putative transport protein
Length = 591
Score = 25.0 bits (53), Expect = 6.4
Identities = 20/80 (25%), Positives = 33/80 (41%), Gaps = 3/80 (3%)
Query: 29 GASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASNDKVTLAGYCPVSEDLEPCRW 88
G+ ++P + A G + + PKN V FG AS+ L + + + W
Sbjct: 155 GSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGW 214
Query: 89 EI---LPAVESNAPQFRVVF 105
+I +PA+ F VF
Sbjct: 215 KIGFGIPAILMLLAGFLFVF 234
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.311 0.127 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,450,996
Number of Sequences: 26719
Number of extensions: 87480
Number of successful extensions: 206
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 193
Number of HSP's gapped (non-prelim): 24
length of query: 105
length of database: 11,318,596
effective HSP length: 81
effective length of query: 24
effective length of database: 9,154,357
effective search space: 219704568
effective search space used: 219704568
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144516.12