BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144513.18 + phase: 0
(107 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09130 ClpP protease complex subunit ClpR3 75 7e-15
At1g71980 unknown protein 30 0.15
At5g06680 gamma-tubulin interacting protein-like 28 0.76
At2g16230 putative beta-1,3-glucanase 27 1.3
At5g53870 predicted GPI-anchored protein 27 1.7
At5g36210 acyl-peptide hydrolase-like 27 1.7
At5g66480 unknown protein 27 2.2
At1g77880 hypothetical protein 26 2.9
At1g45160 similar to IRE homolog 1 dbj|BAA89784.1 26 2.9
At1g23230 conserved hypothetical protein 26 2.9
At3g50750 unknown protein 26 3.8
At3g13150 hypothetical protein 26 3.8
At3g08670 hypothetical protein 26 3.8
At3g03580 unknown protein (At3g03580) 26 3.8
At1g60020 hypothetical protein 26 3.8
At5g49710 putative protein 25 4.9
At5g16110 unknown protein 25 4.9
At5g10170 myo-inositol-1-phosphate synthase -like protein 25 4.9
At3g10310 kinesin-like protein 25 4.9
At2g48130 predicted GPI-anchored protein 25 4.9
>At1g09130 ClpP protease complex subunit ClpR3
Length = 330
Score = 74.7 bits (182), Expect = 7e-15
Identities = 50/110 (45%), Positives = 65/110 (58%), Gaps = 18/110 (16%)
Query: 1 MATCFRVPISMPTSMPASSTRFTP-----------TRLRTLR-TVNAISKSKIPF---NP 45
MA+C + SM + +P SS+ F+P L+T R A + +KIP NP
Sbjct: 1 MASCLQA--SMNSLLPRSSS-FSPHPPLSSNSSGRRNLKTFRYAFRAKASAKIPMPPINP 57
Query: 46 NDPFLSKLASVAESSPETLFNPSSSPDNLPFLDIFDSPQLMATPAQVSNS 95
DPFLS LAS+A +SPE L N + D P+LDIFDSPQLM++PAQV S
Sbjct: 58 KDPFLSTLASIAANSPEKLLNRPVNADVPPYLDIFDSPQLMSSPAQVERS 107
>At1g71980 unknown protein
Length = 448
Score = 30.4 bits (67), Expect = 0.15
Identities = 24/94 (25%), Positives = 48/94 (50%), Gaps = 3/94 (3%)
Query: 14 SMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSSSPDN 73
S +SS R +P + +V+ ++ P +P+ S ++ +A SP++L P+ SP N
Sbjct: 342 SFQSSSNRRSPPISVSRSSVDLRQQAASP-SPSPSQRSYISHMA--SPQSLGYPTISPFN 398
Query: 74 LPFLDIFDSPQLMATPAQVSNSNFSFHFLQIEEN 107
++ + A+PA +SN+ + L+ E+
Sbjct: 399 TRYMSPYRPSPSNASPAMAGSSNYPLNPLRYSES 432
>At5g06680 gamma-tubulin interacting protein-like
Length = 838
Score = 28.1 bits (61), Expect = 0.76
Identities = 22/82 (26%), Positives = 33/82 (39%), Gaps = 4/82 (4%)
Query: 23 TPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSSSPDNLPFLDIFDS 82
+P L+TLR I S++ L ++AES L S D L F D++
Sbjct: 34 SPAFLKTLRYAFRILSSRL----TPSVLPDATAIAESLKRRLATQGKSSDALAFADLYTK 89
Query: 83 PQLMATPAQVSNSNFSFHFLQI 104
P V+N + L+I
Sbjct: 90 FASKTGPGSVNNKWALVYLLKI 111
>At2g16230 putative beta-1,3-glucanase
Length = 456
Score = 27.3 bits (59), Expect = 1.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Query: 40 KIPFNPNDPFLSKLASVAESSPETLFNPSS 69
K P NP D SK A + +P LF+ SS
Sbjct: 421 KSPENPTDCDFSKTARITSENPSKLFSSSS 450
>At5g53870 predicted GPI-anchored protein
Length = 370
Score = 26.9 bits (58), Expect = 1.7
Identities = 23/94 (24%), Positives = 36/94 (37%), Gaps = 6/94 (6%)
Query: 8 PISMPTSMPASSTRFTPTRLRTLRTVNAISKSKI------PFNPNDPFLSKLASVAESSP 61
P P+ PA + +P + +A S S P P P S + + ++P
Sbjct: 233 PAHTPSHSPAHAPSHSPAHAPSHSPAHAPSHSPAHSPSHSPATPKSPSPSSSPAQSPATP 292
Query: 62 ETLFNPSSSPDNLPFLDIFDSPQLMATPAQVSNS 95
+ S SP + P D +P +TP S S
Sbjct: 293 SPMTPQSPSPVSSPSPDQSAAPSDQSTPLAPSPS 326
>At5g36210 acyl-peptide hydrolase-like
Length = 678
Score = 26.9 bits (58), Expect = 1.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Query: 68 SSSPDNLPFLDIFDSPQLMATPAQVSNSNFSFHF 101
SSSPD L + F P+L+ P +V N +F
Sbjct: 391 SSSPDVLKYKAYFSVPELIEFPTEVPGQNAYAYF 424
>At5g66480 unknown protein
Length = 444
Score = 26.6 bits (57), Expect = 2.2
Identities = 16/53 (30%), Positives = 21/53 (39%)
Query: 44 NPNDPFLSKLASVAESSPETLFNPSSSPDNLPFLDIFDSPQLMATPAQVSNSN 96
N SK S +S P L + S P F SP L AT ++ + N
Sbjct: 14 NSGTSLSSKFPSTLQSKPSILNDESKQPKEKTFTRPQMSPSLYATTKEIPHPN 66
>At1g77880 hypothetical protein
Length = 130
Score = 26.2 bits (56), Expect = 2.9
Identities = 19/76 (25%), Positives = 33/76 (43%), Gaps = 5/76 (6%)
Query: 30 LRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSSSPDNLPFLDIFDSPQLMATP 89
L + + K IP+ P+D L+ LA ++ L+ P+ S + F + S +L T
Sbjct: 10 LSSTSEAEKVSIPYLPDDLLLNCLARISR-----LYYPTLSLVSKRFRSLLASTELYETR 64
Query: 90 AQVSNSNFSFHFLQIE 105
+ S +F E
Sbjct: 65 RLLGTSESCLYFFMTE 80
>At1g45160 similar to IRE homolog 1 dbj|BAA89784.1
Length = 1067
Score = 26.2 bits (56), Expect = 2.9
Identities = 22/62 (35%), Positives = 29/62 (46%), Gaps = 11/62 (17%)
Query: 44 NPNDPFLSKLASVAESSPETLFNPSSSPDNLPFLD------IFDSPQLMATPAQVSNSNF 97
N F+S+ + + S ET N S+PD+ LD FDSP P +S NF
Sbjct: 978 NDTSYFVSRFSESSCSDTETGNNSGSNPDSGDELDECTNLEKFDSP-----PYYLSLINF 1032
Query: 98 SF 99
SF
Sbjct: 1033 SF 1034
>At1g23230 conserved hypothetical protein
Length = 1615
Score = 26.2 bits (56), Expect = 2.9
Identities = 22/97 (22%), Positives = 38/97 (38%), Gaps = 9/97 (9%)
Query: 13 TSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSSSPD 72
+++P T + LR V +++ ++ F P L +LAS TLFN +
Sbjct: 1463 SNLPQEWLDGTNAIITNLRPVTSVAMLRVVFRIMGPLLPRLAST-----HTLFNKTLMLL 1517
Query: 73 NLPFLDIFDSPQLMATPAQVSN----SNFSFHFLQIE 105
+D+F P + S +F H + E
Sbjct: 1518 LSALVDVFGKTAQTTAPVEASQIADLIDFLHHIIHYE 1554
>At3g50750 unknown protein
Length = 276
Score = 25.8 bits (55), Expect = 3.8
Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 4/70 (5%)
Query: 2 ATCFRVPISMPTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSP 61
++ F+ PI + P+SS+ +PTR N S IP+ N LA + S+
Sbjct: 100 SSAFQSPIPSYQASPSSSSYPSPTRF----DPNQSSTYLIPYLQNLASSGNLAPLRISNS 155
Query: 62 ETLFNPSSSP 71
+ P SSP
Sbjct: 156 APVTPPISSP 165
>At3g13150 hypothetical protein
Length = 551
Score = 25.8 bits (55), Expect = 3.8
Identities = 27/86 (31%), Positives = 40/86 (46%), Gaps = 10/86 (11%)
Query: 13 TSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSSSPD 72
TS P SS+ T + L +L S S + D +S SSP++ + SSSPD
Sbjct: 425 TSTPVSSSPDTSSFLLSLSLAADSSSSDSDSSSPDS-----SSSVSSSPDSSSSVSSSPD 479
Query: 73 NLPFLDIFDSPQLMATPAQVSNSNFS 98
+ + SP + + VS+S FS
Sbjct: 480 S--YSSFSSSPD---SSSSVSSSLFS 500
>At3g08670 hypothetical protein
Length = 567
Score = 25.8 bits (55), Expect = 3.8
Identities = 18/79 (22%), Positives = 36/79 (44%)
Query: 10 SMPTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVAESSPETLFNPSS 69
S PT ++S TP+R+R + +++ K++ + + ++ SSP + + +
Sbjct: 207 STPTRTSSASRSSTPSRIRPGSSSSSMDKARPSLSSRPSTPTSRPQLSASSPNIIASRPN 266
Query: 70 SPDNLPFLDIFDSPQLMAT 88
S + P S L AT
Sbjct: 267 SRPSTPTRRSPSSTSLSAT 285
>At3g03580 unknown protein (At3g03580)
Length = 882
Score = 25.8 bits (55), Expect = 3.8
Identities = 19/51 (37%), Positives = 25/51 (48%), Gaps = 1/51 (1%)
Query: 7 VPISMPTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLASVA 57
+PI S+ AS R T + T +S+ I NP+DP S LAS A
Sbjct: 672 MPIKPDASIWASVLRACRTS-GDMETAERVSRRIIELNPDDPGYSILASNA 721
>At1g60020 hypothetical protein
Length = 1194
Score = 25.8 bits (55), Expect = 3.8
Identities = 26/95 (27%), Positives = 34/95 (35%), Gaps = 7/95 (7%)
Query: 8 PISMPTSMPASSTRFTPTRLRTLR-------TVNAISKSKIPFNPNDPFLSKLASVAESS 60
P SM + + SS +F+ R T S S P P SK S SS
Sbjct: 766 PPSMSSPVMTSSPQFSSNRDSTTLHGDYSHVDYGLSSPSNPPGPITSPTTSKSPSEPTSS 825
Query: 61 PETLFNPSSSPDNLPFLDIFDSPQLMATPAQVSNS 95
P P+ +P N P + + Q SNS
Sbjct: 826 PSHSNQPNKTPPNSPSSSSSSPTPIPSPSPQSSNS 860
>At5g49710 putative protein
Length = 208
Score = 25.4 bits (54), Expect = 4.9
Identities = 14/35 (40%), Positives = 20/35 (57%)
Query: 47 DPFLSKLASVAESSPETLFNPSSSPDNLPFLDIFD 81
D FL K+ S S+P+ L SSS D + D++D
Sbjct: 91 DDFLEKIESAQVSAPQHLAEDSSSWDVVSEDDLWD 125
>At5g16110 unknown protein
Length = 178
Score = 25.4 bits (54), Expect = 4.9
Identities = 11/29 (37%), Positives = 16/29 (54%)
Query: 42 PFNPNDPFLSKLASVAESSPETLFNPSSS 70
P +PN PFL ++ SP + + SSS
Sbjct: 114 PISPNSPFLQPYSATGFPSPSSSSSSSSS 142
>At5g10170 myo-inositol-1-phosphate synthase -like protein
Length = 510
Score = 25.4 bits (54), Expect = 4.9
Identities = 20/67 (29%), Positives = 32/67 (46%), Gaps = 6/67 (8%)
Query: 30 LRTVNAISKSKIPFNPNDPFLSKLASV----AESSPETLFNPSSSPDNLPFLDIFDSPQL 85
L T N S + ND + ++S+ AE SP TL+ + +N+PF++ SPQ
Sbjct: 228 LWTANTERYSNVVVGLNDTTENLMSSLDKDEAEISPSTLYAIACVLENVPFIN--GSPQN 285
Query: 86 MATPAQV 92
P +
Sbjct: 286 TFVPGLI 292
>At3g10310 kinesin-like protein
Length = 897
Score = 25.4 bits (54), Expect = 4.9
Identities = 18/47 (38%), Positives = 21/47 (44%), Gaps = 1/47 (2%)
Query: 9 ISMPTSMPASSTRFTPTRLRTLRTVNAISKSKIPFNPNDPFLSKLAS 55
I P S P ++T TP RLR L N S +K S LAS
Sbjct: 670 IKSPFSRPIATTERTPPRLRRLSIENC-SSTKANLEDRRGIKSPLAS 715
>At2g48130 predicted GPI-anchored protein
Length = 183
Score = 25.4 bits (54), Expect = 4.9
Identities = 11/27 (40%), Positives = 18/27 (65%)
Query: 45 PNDPFLSKLASVAESSPETLFNPSSSP 71
P+ P S+L SV +SSP+ + + +SP
Sbjct: 51 PSQPCCSRLDSVIKSSPQCICSAVNSP 77
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.129 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,413,661
Number of Sequences: 26719
Number of extensions: 94215
Number of successful extensions: 355
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 333
Number of HSP's gapped (non-prelim): 41
length of query: 107
length of database: 11,318,596
effective HSP length: 83
effective length of query: 24
effective length of database: 9,100,919
effective search space: 218422056
effective search space used: 218422056
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144513.18