BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144481.8 + phase: 0 /pseudo
(119 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g23410 unknown protein 135 2e-33
At4g28570 unknown protein 131 5e-32
At1g03990 unknown protein 115 3e-27
At4g19380 unknown protein 81 9e-17
At5g49555 unknown protein 36 0.003
At1g06820 unknown protein 35 0.006
At5g24155 putative protein 33 0.017
At2g22830 putative squalene epoxidase 33 0.017
At5g24160 squalene monooxygenase 1,2 (squalene epoxidase 1,2) (s... 33 0.023
At5g24150 squalene monooxygenase 33 0.023
At3g62670 putative protein 33 0.023
At3g17240 lipoamide dehydrogenase precursor 33 0.023
At1g62830 flavin-containing amine oxidase 33 0.023
At3g16950 dihydrolipoamide dehydrogenase lpd1 33 0.030
At2g24580 putative sarcosine oxidase 32 0.051
At1g48030 lipoamide dehydrogenase precursor 32 0.051
At3g13682 hypothetical protein 32 0.066
At1g74470 geranylgeranyl reductase 31 0.086
At1g62580 flavin-containing monooxygenase form 2 like protein 31 0.086
At1g57770 unknown protein 31 0.086
>At3g23410 unknown protein
Length = 746
Score = 135 bits (341), Expect = 2e-33
Identities = 67/107 (62%), Positives = 84/107 (77%), Gaps = 1/107 (0%)
Query: 6 NGENQAWEAIGYHVDNDEST-SNIQKERPLEKGIVEAMNEDDLSLAQSLSNKGLEIDIDA 64
NGEN AWEAIGY V+ DE+ S ERPLEKGIVE M E + +L +SL++KGLE +D
Sbjct: 165 NGENPAWEAIGYRVNPDENKPSETHNERPLEKGIVETMEETEQTLLESLAHKGLEAVLDT 224
Query: 65 NSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRD 111
+ +++KCDVV+VGSG GGGVAA+VLA SGLKV+VLEKG+YFTP +
Sbjct: 225 EHDAIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSE 271
>At4g28570 unknown protein
Length = 748
Score = 131 bits (330), Expect = 5e-32
Identities = 66/117 (56%), Positives = 85/117 (72%), Gaps = 5/117 (4%)
Query: 2 QYDENGENQAWEAIGYHVDNDESTSNI-----QKERPLEKGIVEAMNEDDLSLAQSLSNK 56
Q DEN +N A EAIGY +D E +SN +K RPLEKGI+E M+E D+++ QSL+ K
Sbjct: 162 QTDENLKNPALEAIGYCIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEK 221
Query: 57 GLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
G+ + D N+ +++CD V+VGSG GGGVAAA LA +GLKVLVLEKGNYFT D+S
Sbjct: 222 GVHVARDDGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYS 278
>At1g03990 unknown protein
Length = 559
Score = 115 bits (289), Expect = 3e-27
Identities = 50/86 (58%), Positives = 70/86 (81%)
Query: 29 QKERPLEKGIVEAMNEDDLSLAQSLSNKGLEIDIDANSNILKVKCDVVIVGSGCGGGVAA 88
QKERPL++GI+E ED++++ Q + NKGL++ D + K++CD V+VGSGCGGGVAA
Sbjct: 3 QKERPLDEGIIETAKEDEMTIKQRMINKGLKVTEDRERDTYKIECDAVVVGSGCGGGVAA 62
Query: 89 AVLASSGLKVLVLEKGNYFTPRDFSS 114
A+LA SGL+V+V+EKGNYF PRD+S+
Sbjct: 63 AILAKSGLRVVVIEKGNYFAPRDYSA 88
Score = 26.9 bits (58), Expect = 1.6
Identities = 25/94 (26%), Positives = 38/94 (39%), Gaps = 24/94 (25%)
Query: 38 IVEAMNEDDLSLAQSLSNKGLEIDIDANSNI----------------------LKVKCDV 75
+V+A+N + + L Q + K + D DAN L++ V
Sbjct: 226 LVDAVNNNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAASLSHQTRKKLQINAKV 285
Query: 76 VIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTP 109
IV CG +LASSGLK + +G + P
Sbjct: 286 TIVA--CGSLKTPGLLASSGLKNSNISRGLHIHP 317
>At4g19380 unknown protein
Length = 678
Score = 80.9 bits (198), Expect = 9e-17
Identities = 48/125 (38%), Positives = 73/125 (58%), Gaps = 22/125 (17%)
Query: 4 DENGENQAWEAIGY------HVDNDESTSNIQKER-------PLEKGIVEAMNEDDLSLA 50
DE G N AW+AIGY H D++ + +K++ PL GIV+ + + ++
Sbjct: 87 DEKGRNLAWKAIGYNGPSPDHSDHEVELNEEKKKKKPEEIFGPLYNGIVDLKSPRE-AVE 145
Query: 51 QSLSNKGLEIDIDA-NSN-------ILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLE 102
+ L+ +G + N+N ++K++CD V+VGSG GGGVAA VLA +G KVLV+E
Sbjct: 146 KKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVGSGSGGGVAAGVLAKAGYKVLVIE 205
Query: 103 KGNYF 107
GNY+
Sbjct: 206 SGNYY 210
>At5g49555 unknown protein
Length = 556
Score = 36.2 bits (82), Expect = 0.003
Identities = 19/38 (50%), Positives = 23/38 (60%)
Query: 66 SNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
S + K K D V++G G G AAA LA GL V VLE+
Sbjct: 7 STLPKKKWDAVVIGGGHNGLTAAAYLARGGLSVAVLER 44
>At1g06820 unknown protein
Length = 595
Score = 35.0 bits (79), Expect = 0.006
Identities = 17/30 (56%), Positives = 21/30 (69%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
D +++GSG GG VAA LA +VLVLEK
Sbjct: 79 DAIVIGSGIGGLVAATQLAVKEARVLVLEK 108
>At5g24155 putative protein
Length = 121
Score = 33.5 bits (75), Expect = 0.017
Identities = 15/30 (50%), Positives = 20/30 (66%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
DV+IVG+G GG A LA G +VL +E+
Sbjct: 49 DVIIVGAGVGGSALAYSLAKDGRRVLAIER 78
>At2g22830 putative squalene epoxidase
Length = 585
Score = 33.5 bits (75), Expect = 0.017
Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 14/61 (22%)
Query: 55 NKGLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSS 114
+K LE ++D+ + DV+IVG+G G A L G +V V+E RDFS
Sbjct: 112 SKNLETEVDSGT-------DVIIVGAGVAGSALAHTLGKEGRRVHVIE-------RDFSE 157
Query: 115 R 115
+
Sbjct: 158 Q 158
>At5g24160 squalene monooxygenase 1,2 (squalene epoxidase 1,2) (se
1,2) (sp|O65402)
Length = 517
Score = 33.1 bits (74), Expect = 0.023
Identities = 15/30 (50%), Positives = 20/30 (66%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
DV+IVG+G GG A LA G +V V+E+
Sbjct: 47 DVIIVGAGVGGSALAYALAKDGRRVHVIER 76
>At5g24150 squalene monooxygenase
Length = 516
Score = 33.1 bits (74), Expect = 0.023
Identities = 15/30 (50%), Positives = 20/30 (66%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
DV+IVG+G GG A LA G +V V+E+
Sbjct: 47 DVIIVGAGVGGSALAYALAKDGRRVHVIER 76
>At3g62670 putative protein
Length = 426
Score = 33.1 bits (74), Expect = 0.023
Identities = 16/47 (34%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Query: 22 DESTSNIQKERPLEKGIVEAMNEDDLSLAQSLSNKGLEIDIDANSNI 68
DE++ N++ E P + GI +N+DD + SN+ L + D+NS++
Sbjct: 9 DENSRNLRNEIPCDDGIASPINDDDEEFL-TKSNRVLLVGADSNSSL 54
>At3g17240 lipoamide dehydrogenase precursor
Length = 507
Score = 33.1 bits (74), Expect = 0.023
Identities = 17/30 (56%), Positives = 19/30 (62%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
DVVI+G G GG VAA A GLK +EK
Sbjct: 45 DVVIIGGGPGGYVAAIKAAQLGLKTTCIEK 74
>At1g62830 flavin-containing amine oxidase
Length = 844
Score = 33.1 bits (74), Expect = 0.023
Identities = 17/29 (58%), Positives = 21/29 (71%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLE 102
+VV+VG+G G VAA L S G +VLVLE
Sbjct: 267 NVVVVGAGLAGLVAARQLLSMGFRVLVLE 295
>At3g16950 dihydrolipoamide dehydrogenase lpd1
Length = 570
Score = 32.7 bits (73), Expect = 0.030
Identities = 19/57 (33%), Positives = 28/57 (48%), Gaps = 5/57 (8%)
Query: 51 QSLSNKGLEIDIDANSN-----ILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLE 102
Q LS K ++ A+SN D++I+G+G GG AA GLK ++E
Sbjct: 60 QFLSRKSFQVSASASSNGNGAPPKSFDYDLIIIGAGVGGHGAALHAVEKGLKTAIIE 116
>At2g24580 putative sarcosine oxidase
Length = 416
Score = 32.0 bits (71), Expect = 0.051
Identities = 17/40 (42%), Positives = 24/40 (59%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
DV++VG+G G AA LA G K L+LE+ ++ R S
Sbjct: 10 DVIVVGAGVMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSS 49
>At1g48030 lipoamide dehydrogenase precursor
Length = 507
Score = 32.0 bits (71), Expect = 0.051
Identities = 16/30 (53%), Positives = 19/30 (63%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
DVVI+G G GG VAA + GLK +EK
Sbjct: 45 DVVIIGGGPGGYVAAIKASQLGLKTTCIEK 74
>At3g13682 hypothetical protein
Length = 746
Score = 31.6 bits (70), Expect = 0.066
Identities = 16/28 (57%), Positives = 19/28 (67%)
Query: 75 VVIVGSGCGGGVAAAVLASSGLKVLVLE 102
V++VG+G G AA L S G KVLVLE
Sbjct: 162 VIVVGAGLAGLAAARQLLSFGFKVLVLE 189
>At1g74470 geranylgeranyl reductase
Length = 467
Score = 31.2 bits (69), Expect = 0.086
Identities = 12/32 (37%), Positives = 20/32 (62%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
K V ++G G GG AA LA G++ +++E+
Sbjct: 55 KLRVAVIGGGPAGGAAAETLAQGGIETILIER 86
>At1g62580 flavin-containing monooxygenase form 2 like protein
Length = 497
Score = 31.2 bits (69), Expect = 0.086
Identities = 14/32 (43%), Positives = 20/32 (61%)
Query: 75 VVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
V ++G+G G VAA L G V+V E+GN+
Sbjct: 14 VAVIGAGAAGLVAARELRREGHSVVVFERGNH 45
>At1g57770 unknown protein
Length = 574
Score = 31.2 bits (69), Expect = 0.086
Identities = 15/35 (42%), Positives = 22/35 (62%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
+ DVV++GSG GG A+LA V+VLE ++
Sbjct: 52 EADVVVIGSGIGGLCCGALLARYDQDVIVLESHDH 86
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.313 0.134 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,816,664
Number of Sequences: 26719
Number of extensions: 112920
Number of successful extensions: 426
Number of sequences better than 10.0: 112
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 330
Number of HSP's gapped (non-prelim): 113
length of query: 119
length of database: 11,318,596
effective HSP length: 95
effective length of query: 24
effective length of database: 8,780,291
effective search space: 210726984
effective search space used: 210726984
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144481.8