BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.10 - phase: 0 /pseudo
(200 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g11890 pseudogene 227 4e-60
At5g61140 RNA helicase - like protein 30 0.65
At3g02490 unknown protein 30 0.65
At4g08710 putative protein 28 3.2
At1g69390 unknown protein 28 3.2
At3g49730 putative protein 27 5.5
At2g41090 calcium binding protein (CaBP-22) 27 5.5
At1g69990 hypothetical protein 27 5.5
At5g57090 root gravitropism control protein (PIN2) 27 7.2
At5g43940 alcohol dehydrogenase (EC 1.1.1.1) class III (pir||S71... 27 7.2
At3g18020 hypothetical protein 27 7.2
At1g07740 hypothetical protein 27 7.2
At5g13770 putative protein 27 9.4
At5g08770 putative protein 27 9.4
At4g10070 putative DNA-directed RNA polymerase 27 9.4
>At2g11890 pseudogene
Length = 210
Score = 227 bits (578), Expect = 4e-60
Identities = 115/209 (55%), Positives = 153/209 (73%), Gaps = 9/209 (4%)
Query: 1 MEVEVKLRLPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAILRLRFYGD-- 58
MEVEVKLRL A ++ R+TTLL+P+H+ T Q N FFD ++LS RRA+LRLRF +
Sbjct: 1 MEVEVKLRLLTAAAHLRLTTLLTPYHLKTLHQRNTFFDTPKNDLSLRRAVLRLRFLQNAA 60
Query: 59 -------DERCVVSLKAKAVLVDGVSRVEEDEEDLDPKIGRDCVDEPGKLGLVESRIMGR 111
RC+VSLKAK L +G+SRVEEDEE+++ IG++CV+ P KL + SR++ R
Sbjct: 61 VSAASPSPPRCIVSLKAKPTLANGISRVEEDEEEIEYWIGKECVESPAKLSDIGSRVLKR 120
Query: 112 VKEEFGVVGENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKR 171
VKEE+G GFV LGGF+NVRNVY+W+G+KLEVDET +DFG +EIECE+ +PE K
Sbjct: 121 VKEEYGFNDFLGFVCLGGFENVRNVYEWRGVKLEVDETKYDFGNCYEIECETEEPERVKT 180
Query: 172 ILEEFLKENGIDYSYSVASKFAIFRAGKL 200
++EEFL E I++S S +KFA+FR+GKL
Sbjct: 181 MIEEFLTEEKIEFSNSDMTKFAVFRSGKL 209
>At5g61140 RNA helicase - like protein
Length = 2139
Score = 30.4 bits (67), Expect = 0.65
Identities = 21/78 (26%), Positives = 40/78 (50%), Gaps = 6/78 (7%)
Query: 25 FHVITHRQHNLFFD---GAGSELSSRRAILRLRFYGDDERCVVSLKAKAVLVDGVSRVEE 81
FHV+ H +N+ G+G +S+ A+LRL D + V KA++ + R+ +
Sbjct: 1353 FHVLYHTDNNVLVGAPTGSGKTISAELAMLRLFSTQPDMKVVYIAPLKAIVRE---RMND 1409
Query: 82 DEEDLDPKIGRDCVDEPG 99
++ L +G++ V+ G
Sbjct: 1410 WKKHLVAPLGKEMVEMTG 1427
>At3g02490 unknown protein
Length = 665
Score = 30.4 bits (67), Expect = 0.65
Identities = 23/89 (25%), Positives = 39/89 (42%), Gaps = 13/89 (14%)
Query: 110 GRVKEEFGVVGENGFVGLGGFKNV-RNVYDWKGLKLEVDE---------THFD---FGTL 156
G+ E + E G+V G ++V + KG K E +E H D +L
Sbjct: 407 GQSNEVLKAMNEGGYVPSGDLQSVIASGLSRKGKKDEANELVNFMEASGNHLDDKAMASL 466
Query: 157 FEIECESSDPEEAKRILEEFLKENGIDYS 185
E C++ D EEA ++ + + G+ Y+
Sbjct: 467 VEGHCDAKDLEEASECFKKMIGKEGVSYA 495
>At4g08710 putative protein
Length = 715
Score = 28.1 bits (61), Expect = 3.2
Identities = 15/33 (45%), Positives = 19/33 (57%)
Query: 57 GDDERCVVSLKAKAVLVDGVSRVEEDEEDLDPK 89
GD E VV K DGVS+ +E E++ DPK
Sbjct: 650 GDQEEDVVQEKDGDQEEDGVSKEKEQEKEKDPK 682
>At1g69390 unknown protein
Length = 229
Score = 28.1 bits (61), Expect = 3.2
Identities = 28/95 (29%), Positives = 47/95 (49%), Gaps = 5/95 (5%)
Query: 45 SSRRAILRLRFYGDDERCVVSLKAKAVLVDGVSRVEED--EEDLDPKIGRDCVDEPGKLG 102
++R A RL+ +RC VS +AK +V+ + D E + + K+ + V G LG
Sbjct: 123 NARIAKQRLKMILFSDRCDVSDEAKRKIVNNIIHALSDFVEIESEEKVQLN-VSTDGDLG 181
Query: 103 LVESRI--MGRVKEEFGVVGENGFVGLGGFKNVRN 135
+ S + RVK E+ V E G + +K+ R+
Sbjct: 182 TIYSVTVPVRRVKPEYQDVDEAGTITNVEYKDTRD 216
>At3g49730 putative protein
Length = 1184
Score = 27.3 bits (59), Expect = 5.5
Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Query: 144 LEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKENGIDYSYSVASKFAIFRAGKL 200
LE DE + FG L + C++ +EA ++ E+ ++ + Y + + R GKL
Sbjct: 198 LEPDE--YVFGCLLDALCKNGSVKEASKVFEDMREKFPPNLRYFTSLLYGWCREGKL 252
>At2g41090 calcium binding protein (CaBP-22)
Length = 191
Score = 27.3 bits (59), Expect = 5.5
Identities = 12/39 (30%), Positives = 22/39 (55%), Gaps = 2/39 (5%)
Query: 112 VKEEFGV--VGENGFVGLGGFKNVRNVYDWKGLKLEVDE 148
+K++F + + +NGF+ + VR + WK E+DE
Sbjct: 83 LKKDFRLFDIDKNGFISAAEMRYVRTILRWKQTDEEIDE 121
>At1g69990 hypothetical protein
Length = 591
Score = 27.3 bits (59), Expect = 5.5
Identities = 15/49 (30%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Query: 100 KLGLVESRIMGRVKEEFGVVGENGFVGLGGF--KNVRNVYDWKGLKLEV 146
+L L ++ + G + E GE+GF G GG K + N + G L +
Sbjct: 166 RLSLADNDLSGSIPSELSHYGEDGFRGNGGLCGKPLSNCGSFNGKNLTI 214
>At5g57090 root gravitropism control protein (PIN2)
Length = 647
Score = 26.9 bits (58), Expect = 7.2
Identities = 14/41 (34%), Positives = 19/41 (46%)
Query: 110 GRVKEEFGVVGENGFVGLGGFKNVRNVYDWKGLKLEVDETH 150
G K+E G G G VG+GG N++ W V E +
Sbjct: 359 GVKKKESGGGGSGGGVGVGGQNKEMNMFVWSSSASPVSEAN 399
>At5g43940 alcohol dehydrogenase (EC 1.1.1.1) class III
(pir||S71244)
Length = 379
Score = 26.9 bits (58), Expect = 7.2
Identities = 16/78 (20%), Positives = 37/78 (46%), Gaps = 4/78 (5%)
Query: 123 GFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEF---LKE 179
G VGL + + + + +++D ++ F + E +P++ + ++E L +
Sbjct: 204 GTVGLAVAEGAKTAGASRIIGIDIDSKKYETAKKFGVN-EFVNPKDHDKPIQEVIVDLTD 262
Query: 180 NGIDYSYSVASKFAIFRA 197
G+DYS+ ++ RA
Sbjct: 263 GGVDYSFECIGNVSVMRA 280
>At3g18020 hypothetical protein
Length = 688
Score = 26.9 bits (58), Expect = 7.2
Identities = 12/44 (27%), Positives = 23/44 (52%)
Query: 140 KGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKENGID 183
+G + E + + + L E C+ D +A+ +LE L++ G D
Sbjct: 367 EGSEFEFFPSEYTYKLLMESLCKELDTGKARNVLELMLRKEGAD 410
>At1g07740 hypothetical protein
Length = 459
Score = 26.9 bits (58), Expect = 7.2
Identities = 15/57 (26%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
Query: 123 GFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKE 179
GF+ ++ V+D + L++EV + + +L C + D +AK +LE+ +K+
Sbjct: 195 GFLDKCDWEAACKVFD-EMLEMEVQPSVVTYNSLIGFLCRNDDMGKAKSLLEDMIKK 250
>At5g13770 putative protein
Length = 563
Score = 26.6 bits (57), Expect = 9.4
Identities = 14/58 (24%), Positives = 24/58 (41%), Gaps = 1/58 (1%)
Query: 122 NGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKE 179
NGF GF VY+W +K E + + C +A+ + +E +K+
Sbjct: 315 NGFSKQRGFAEAVKVYEW-AMKEECEAGQVTYAIAINAYCRLEKYNKAEMLFDEMVKK 371
>At5g08770 putative protein
Length = 154
Score = 26.6 bits (57), Expect = 9.4
Identities = 19/74 (25%), Positives = 32/74 (42%), Gaps = 3/74 (4%)
Query: 74 DGVSRVEEDEEDLDPKIGRDCVDEPGKLGLVESRIMGRVKEEFGVVGENGFVGLGGFKNV 133
D V +E+E++ D G + P L +V +R + + G NG + GF
Sbjct: 73 DHVEGCDEEEKESD---GVGYISIPRSLPIVANRYVSEINSRVGTKYSNGNLPDTGFSVF 129
Query: 134 RNVYDWKGLKLEVD 147
NV G+ E++
Sbjct: 130 INVVCIAGIMSEIE 143
>At4g10070 putative DNA-directed RNA polymerase
Length = 748
Score = 26.6 bits (57), Expect = 9.4
Identities = 16/49 (32%), Positives = 28/49 (56%), Gaps = 1/49 (2%)
Query: 142 LKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKENGIDYSYSVAS 190
LK ++DE + G + + ++SD +AKR E ++G+ S S+AS
Sbjct: 16 LKRKLDEVELN-GNIVDDSNQTSDSSQAKRAKLEDEAQDGLGVSVSLAS 63
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.141 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,776,030
Number of Sequences: 26719
Number of extensions: 217836
Number of successful extensions: 650
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 643
Number of HSP's gapped (non-prelim): 16
length of query: 200
length of database: 11,318,596
effective HSP length: 94
effective length of query: 106
effective length of database: 8,807,010
effective search space: 933543060
effective search space used: 933543060
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144477.10