BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144430.8 - phase: 0 /pseudo
(91 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g22350 putative non-LTR retroelement reverse transcriptase 42 5e-05
At2g17910 putative non-LTR retroelement reverse transcriptase 40 3e-04
At2g16680 putative non-LTR retroelement reverse transcriptase 40 3e-04
At2g07650 putative non-LTR retrolelement reverse transcriptase 39 6e-04
At1g47910 reverse transcriptase, putative 39 6e-04
At2g31080 putative non-LTR retroelement reverse transcriptase 38 0.001
At5g20800 putative protein 37 0.001
At2g41580 putative non-LTR retroelement reverse transcriptase 37 0.002
At2g27870 putative non-LTR retroelement reverse transcriptase 36 0.003
At2g17610 putative non-LTR retroelement reverse transcriptase 36 0.003
At2g07730 putative non-LTR retroelement reverse transcriptase 36 0.004
At2g02650 putative reverse transcriptase 35 0.005
At2g01840 putative non-LTR retroelement reverse transcriptase 35 0.005
At2g15540 putative non-LTR retroelement reverse transcriptase 35 0.006
At3g25270 hypothetical protein 35 0.008
At1g10160 putative reverse transcriptase 35 0.008
At2g34320 putative non-LTR retroelement reverse transcriptase 34 0.011
At3g31420 hypothetical protein 34 0.014
At2g31520 putative non-LTR retroelement reverse transcriptase 33 0.019
At4g29090 putative protein 33 0.025
>At2g22350 putative non-LTR retroelement reverse transcriptase
Length = 321
Score = 42.0 bits (97), Expect = 5e-05
Identities = 26/81 (32%), Positives = 36/81 (44%), Gaps = 3/81 (3%)
Query: 3 HKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW-LAISFDNINFFLEHNLGWWIIIGL 61
++R +S T VC C E + H L DC +W + D I F +L WI L
Sbjct: 25 YRRHLSDTRVCQICQGGEETILHVLRDCPAMAGIWSRLVPRDQIRQFFTASLLEWIYKNL 84
Query: 62 DRQ--LPHLFLSAVWLLWMAR 80
+ P +F+ AVW W R
Sbjct: 85 RERGSWPTVFVMAVWWGWKWR 105
>At2g17910 putative non-LTR retroelement reverse transcriptase
Length = 1344
Score = 39.7 bits (91), Expect = 3e-04
Identities = 25/88 (28%), Positives = 40/88 (45%), Gaps = 8/88 (9%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW----LAISFDNINFFLEHNLGWWI 57
L RG+ D C C++ +E + H L +C R +W L+ + + + N+ I
Sbjct: 1051 LRTRGIRSDDGCLMCDTENETINHILFECPLARQVWAITHLSSAGSEFSNSVYTNMSRLI 1110
Query: 58 IIGLDRQLPH--LFLS--AVWLLWMARN 81
+ LPH F+S +W LW RN
Sbjct: 1111 DLTQQNDLPHHLRFVSPWILWFLWKNRN 1138
>At2g16680 putative non-LTR retroelement reverse transcriptase
Length = 1319
Score = 39.7 bits (91), Expect = 3e-04
Identities = 25/94 (26%), Positives = 40/94 (41%), Gaps = 18/94 (19%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAIS-------------FDNINFF 48
L RG+ D C C E + H L C F R +W A+S F NIN
Sbjct: 1025 LRSRGIRTADGCLFCKEEIETINHLLFQCPFARQVW-ALSLIQAPATGFGTSIFSNINHV 1083
Query: 49 LEHNLGWWIIIGLDRQLPHLFLSAVWLLWMARNR 82
++++ + G+ R + + +W +W RN+
Sbjct: 1084 IQNSQNF----GIPRHMRTVSPWLLWEIWKNRNK 1113
>At2g07650 putative non-LTR retrolelement reverse transcriptase
Length = 732
Score = 38.5 bits (88), Expect = 6e-04
Identities = 15/44 (34%), Positives = 21/44 (47%)
Query: 3 HKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNIN 46
H+R +S T +C C E + H L DC +WL + D N
Sbjct: 596 HRRHLSTTTICQVCKGAKESIIHVLRDCPAMEGIWLRLISDLAN 639
>At1g47910 reverse transcriptase, putative
Length = 1142
Score = 38.5 bits (88), Expect = 6e-04
Identities = 29/90 (32%), Positives = 44/90 (48%), Gaps = 15/90 (16%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAIS--------FDNINFF--LEH 51
L KRG+ C C ++ E + H L C R +W A+S F + + F L+H
Sbjct: 855 LRKRGILCDKGCVSCGASEESINHTLFQCHPARQIW-ALSQIPTAPGIFPSNSIFTNLDH 913
Query: 52 NLGWWIIIGLDRQLPHLFLSAVWLLWMARN 81
L W I G+D P+ ++ +W +W ARN
Sbjct: 914 -LFWRIPSGVD-SAPYPWI--IWYIWKARN 939
>At2g31080 putative non-LTR retroelement reverse transcriptase
Length = 1231
Score = 37.7 bits (86), Expect = 0.001
Identities = 21/81 (25%), Positives = 33/81 (39%), Gaps = 4/81 (4%)
Query: 4 KRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW-LAISFDNINFFLEHNLGWWIIIGLD 62
+R +S +C+ CN E + H L DC +W + + F +L W+ +D
Sbjct: 935 RRHLSENAICSVCNGAEETILHVLRDCPAMEPIWRRLLPLRRHHEFFSQSLLEWLFTNMD 994
Query: 63 RQ---LPHLFLSAVWLLWMAR 80
P LF +W W R
Sbjct: 995 PVKGIWPTLFGMGIWWAWKWR 1015
>At5g20800 putative protein
Length = 359
Score = 37.4 bits (85), Expect = 0.001
Identities = 25/89 (28%), Positives = 38/89 (42%), Gaps = 13/89 (14%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISF---------DNINFFLEHN 52
L KRG++ C RC +T + + H L C R +W D+I L+H
Sbjct: 154 LRKRGINCDIGCARCGATEKTINHTLFQCHPARQIWALSQIPTVIGIFPSDSIYENLDH- 212
Query: 53 LGWWIIIGLDRQLPHLFLSAVWLLWMARN 81
L W + +D + +W +W ARN
Sbjct: 213 LFWRVQSAVD---SFSYPWIIWYIWKARN 238
>At2g41580 putative non-LTR retroelement reverse transcriptase
Length = 1094
Score = 37.0 bits (84), Expect = 0.002
Identities = 25/93 (26%), Positives = 39/93 (41%), Gaps = 16/93 (17%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNINFFLEHNLGWWIIIGL 61
L RG+ D C+ C +E + H L C R +W + N H G I +
Sbjct: 802 LRTRGVLIEDGCSMCPEKNETLNHILFQCPLARQVWALTPMQSPN----HGFGDSIFTNV 857
Query: 62 DRQL---------PHL-FLS--AVWLLWMARNR 82
+ + PHL ++S +W+LW RN+
Sbjct: 858 NHVIGNCHNTELSPHLRYVSPWIIWILWKNRNK 890
>At2g27870 putative non-LTR retroelement reverse transcriptase
Length = 314
Score = 36.2 bits (82), Expect = 0.003
Identities = 21/85 (24%), Positives = 35/85 (40%), Gaps = 8/85 (9%)
Query: 4 KRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAI--SFDNINFFLEHNLGWWIIIGL 61
+R +S +D+C C + + H L DC +W+ + + FF + L W
Sbjct: 13 RRHLSDSDICQICKGAEKTIIHILRDCPAMEGIWIRLVPAGKRREFFTQSLLEWLFANLG 72
Query: 62 DRQ------LPHLFLSAVWLLWMAR 80
DR+ LF ++W W R
Sbjct: 73 DRRKTCESTWSTLFALSIWWAWKWR 97
>At2g17610 putative non-LTR retroelement reverse transcriptase
Length = 773
Score = 36.2 bits (82), Expect = 0.003
Identities = 14/36 (38%), Positives = 18/36 (49%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW 37
L +R + D C RC SEDV H L C + +W
Sbjct: 528 LKRRRLITDDTCQRCGEASEDVNHLLFQCRVSKEIW 563
>At2g07730 putative non-LTR retroelement reverse transcriptase
Length = 970
Score = 35.8 bits (81), Expect = 0.004
Identities = 23/82 (28%), Positives = 31/82 (37%), Gaps = 8/82 (9%)
Query: 4 KRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW--LAISFDNINFFLEHNLGWWIIIGL 61
+R +S C+ CN E + H L DC +W L FF + W+ L
Sbjct: 676 RRHLSDIATCSVCNGADESILHVLRDCPAMTPIWQRLLPQRRQNEFFSQFE---WLFTNL 732
Query: 62 D---RQLPHLFLSAVWLLWMAR 80
D P LF +W W R
Sbjct: 733 DPAKGDWPTLFSMGIWWAWKWR 754
>At2g02650 putative reverse transcriptase
Length = 365
Score = 35.4 bits (80), Expect = 0.005
Identities = 22/93 (23%), Positives = 39/93 (41%), Gaps = 17/93 (18%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISF-------------DNINFF 48
L R + +C RC E + H + +C + +++W + + DN+N
Sbjct: 64 LRSRNIDADPICQRCCIEEETIHHIMFNCPYTQSVWRSANIIIGNQWGPPSSFEDNLNRL 123
Query: 49 LEHNLGWWIIIGLDRQLPHLFLSAVWLLWMARN 81
++ + LDR LP + W LW +RN
Sbjct: 124 IQLS-KTQTTNSLDRFLPFWIM---WRLWKSRN 152
>At2g01840 putative non-LTR retroelement reverse transcriptase
Length = 1715
Score = 35.4 bits (80), Expect = 0.005
Identities = 23/95 (24%), Positives = 39/95 (40%), Gaps = 9/95 (9%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNINFF-----LEHNLGWW 56
L R + C RC + E + H + C++ + +W + +F N LE N+
Sbjct: 1415 LRNRNIPADPTCQRCCNADETINHIIFTCSYAQVVWRSANFSGSNRLCFTDNLEENIRLI 1474
Query: 57 IIIGLDRQLPHLF----LSAVWLLWMARNRACIAQ 87
+ ++ LP L +W LW +RN Q
Sbjct: 1475 LQGKKNQNLPILNGLMPFWIMWRLWKSRNEYLFQQ 1509
>At2g15540 putative non-LTR retroelement reverse transcriptase
Length = 1225
Score = 35.0 bits (79), Expect = 0.006
Identities = 21/90 (23%), Positives = 36/90 (39%), Gaps = 9/90 (10%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNINFF-----LEHNLGWW 56
L R + VC RC + E + H L C R +W + + L +N +
Sbjct: 953 LFSRHIGTEKVCPRCGAEEESINHLLFLCPPSRQIWALSPIPSSEYIFPRNSLFYNFDFL 1012
Query: 57 IIIG----LDRQLPHLFLSAVWLLWMARNR 82
+ G + + +F +W +W +RNR
Sbjct: 1013 LSRGKEFDIAEDIMEIFPWILWYIWKSRNR 1042
>At3g25270 hypothetical protein
Length = 343
Score = 34.7 bits (78), Expect = 0.008
Identities = 23/95 (24%), Positives = 38/95 (39%), Gaps = 8/95 (8%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDN-----INFFLEHNLGWW 56
L +R + C RC E H DC + + +W A + +E +
Sbjct: 45 LKRRHIRNHPQCHRCCQEDETSQHLFFDCFYAQQVWRASGIPHQELRTTGITMETKMELL 104
Query: 57 IIIGLDRQLPHLFLSAVWL---LWMARNRACIAQE 88
+ L + P LF A+W+ LW +RN+ Q+
Sbjct: 105 LSSCLANRQPQLFNLAIWILWRLWKSRNQLVFQQK 139
>At1g10160 putative reverse transcriptase
Length = 1118
Score = 34.7 bits (78), Expect = 0.008
Identities = 24/88 (27%), Positives = 36/88 (40%), Gaps = 9/88 (10%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW----LAISFDNINFFLEHNLGWWI 57
L + G S C CN E H C F +W A++ + F+ H L W +
Sbjct: 974 LRRWGFSIPPTCVLCNDLDESREHLFFRCQFSSEIWSFFMRALNLNPPPQFM-HCLLWTL 1032
Query: 58 IIGLDRQL----PHLFLSAVWLLWMARN 81
DR + LF ++V+ +W RN
Sbjct: 1033 TASRDRNITLITKLLFHASVYFIWRERN 1060
>At2g34320 putative non-LTR retroelement reverse transcriptase
Length = 292
Score = 34.3 bits (77), Expect = 0.011
Identities = 24/80 (30%), Positives = 34/80 (42%), Gaps = 13/80 (16%)
Query: 13 CTRCNSTSEDVFHCLLDCAFPRNLWL-----AISFDNINFFLEHNLGWWIIIGLDRQLP- 66
C RC + E V H L C F R +W A L NL W ++ L+ ++P
Sbjct: 12 CVRCPDSRETVNHLLFKCCFARLVWAISPIPAYPEGEWTDSLYANLYW--VLNLEVEIPK 69
Query: 67 -----HLFLSAVWLLWMARN 81
+L +W LW +RN
Sbjct: 70 LGKIGNLVPWLLWRLWKSRN 89
>At3g31420 hypothetical protein
Length = 1491
Score = 33.9 bits (76), Expect = 0.014
Identities = 24/97 (24%), Positives = 33/97 (33%), Gaps = 25/97 (25%)
Query: 1 MLHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNINF------------- 47
ML R ++ +C RC E H +C + + W N+ F
Sbjct: 1194 MLRYRHINKQSICKRCCRDEETSQHLFFECDYAKATWRGAGLPNLIFQDSIVTLEEKFRA 1253
Query: 48 ---FLEHNLGWWIIIGLDRQLPHLFLSAVWLLWMARN 81
F +W RQLP L W LW +RN
Sbjct: 1254 MFTFNPSTTNYW------RQLPFWIL---WRLWRSRN 1281
>At2g31520 putative non-LTR retroelement reverse transcriptase
Length = 1524
Score = 33.5 bits (75), Expect = 0.019
Identities = 25/89 (28%), Positives = 34/89 (38%), Gaps = 9/89 (10%)
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNINFFL-----EHNLGWW 56
L RGM +C RC+ +E + H L C F W I L E N+
Sbjct: 1229 LTTRGMRIDPICPRCHRENESINHALFTCPFATMAWWLSDSSLIRNQLMSNDFEENISNI 1288
Query: 57 IIIGLDRQLP--HLFLSA--VWLLWMARN 81
+ D + H L +W +W ARN
Sbjct: 1289 LNFVQDTTMSDFHKLLPVWLIWRIWKARN 1317
>At4g29090 putative protein
Length = 575
Score = 33.1 bits (74), Expect = 0.025
Identities = 26/93 (27%), Positives = 31/93 (32%), Gaps = 23/93 (24%)
Query: 5 RGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFD-----------NINFFLEHNL 53
R +S C RC S E V H L C F R W S +N + NL
Sbjct: 287 RHLSKESACIRCPSCKETVNHLLFKCTFARLTWAISSIPIPLGGEWADSIYVNLYWVFNL 346
Query: 54 G-----WWIIIGLDRQLPHLFLSAVWLLWMARN 81
G W + L +W LW RN
Sbjct: 347 GNGNPQW-------EKASQLVPWLLWRLWKNRN 372
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.331 0.141 0.509
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,332,322
Number of Sequences: 26719
Number of extensions: 84913
Number of successful extensions: 285
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 52
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 224
Number of HSP's gapped (non-prelim): 66
length of query: 91
length of database: 11,318,596
effective HSP length: 67
effective length of query: 24
effective length of database: 9,528,423
effective search space: 228682152
effective search space used: 228682152
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144430.8