BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144345.6 - phase: 0
(360 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g70170 predicted GPI-anchored protein 147 7e-36
At1g24140 metalloproteinase like protein, predicted GPI-anchor 136 2e-32
At1g59970 predicted GPI-anchored protein 127 1e-29
At2g45040 metalloproteinase like protein ,predicted GPI-anchored... 120 1e-27
At4g16640 proteinase-like protein, predicted GPI-anchored protei... 114 1e-25
At3g49220 pectinesterase - like protein 32 0.54
At4g24350 putative protein 30 1.6
At1g50680 hypothetical protein 30 1.6
At1g70650 hypothetical protein 30 2.7
At3g06020 hypothetical protein 28 5.9
At5g14160 putative protein 28 7.7
At3g43540 unknown protein 28 7.7
At2g22980 putative serine carboxypeptidase I 28 7.7
>At1g70170 predicted GPI-anchored protein
Length = 378
Score = 147 bits (372), Expect = 7e-36
Identities = 106/314 (33%), Positives = 163/314 (51%), Gaps = 51/314 (16%)
Query: 81 GVDQIKQYLSDFGYLEQ--SGPFNNTLDQETVLALKTYQRYFNI----QQDTLSEILQHI 134
G+ +IK+Y FGY+ + SG F + D A++ YQ FN+ + D L+ +QHI
Sbjct: 58 GLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALT--IQHI 115
Query: 135 ALPRCGVPDRILKYNL-------TNDISFPK-------------GNQWFPKGTKNLTYGF 174
+PRCG PD + +L T +++F + G +P+ ++LTY F
Sbjct: 116 VIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAF 175
Query: 175 DPRNKIPLDMTNVFRTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDDDGINDVAVG 234
DP+N + ++ +VF A +WS+ T LNFT ++S+ ++I IGFY T D G + G
Sbjct: 176 DPKNPLTEEVKSVFSRAFGRWSDVT-ALNFTLSESFSTSDITIGFY--TGDHGDGEPFDG 232
Query: 235 FTFIVLDSTNVKSGFITLDATKYWALPTEHRGF-------DLETAAMHQIGHLLGLEHSS 287
+ + + SG LDA + W + + F DLE+ A+H+IGHLLGL HSS
Sbjct: 233 VLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSS 292
Query: 288 DNKSIMYPTILPSHQKNVQITDSDNLAIQKLYS------------SSTKANANSDDSSGC 335
+SIMYPTI +K V +T+ D IQ LY S+TK ++ S
Sbjct: 293 VEESIMYPTITTGKRK-VDLTNDDVEGIQYLYGANPNFNGTTSPPSTTKHQRDTGGFSAA 351
Query: 336 FKLFGSSSSLLISL 349
+++ GSS S ++SL
Sbjct: 352 WRIDGSSRSTIVSL 365
>At1g24140 metalloproteinase like protein, predicted GPI-anchor
Length = 384
Score = 136 bits (342), Expect = 2e-32
Identities = 93/285 (32%), Positives = 138/285 (47%), Gaps = 38/285 (13%)
Query: 78 KYKGVDQIKQYLSDFGYLEQ---SGPFNNTLDQETVLALKTYQRYFNIQ-QDTLSEI-LQ 132
KY G+ +KQY FGY+ + SG F + D A++ YQR F + L E+ L+
Sbjct: 57 KYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLK 116
Query: 133 HIALPRCGVPD---------------------RILKYNLTNDISFPKGNQWFPKGTKNLT 171
H+ +PRCG PD R +++ SF G +P+ ++LT
Sbjct: 117 HVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLT 176
Query: 172 YGFDPRNKIPLDMTNVFRTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDDDGINDV 231
Y FDPRN + ++ +VF A T+W T L FT + + ++I IGFY+ DG +
Sbjct: 177 YAFDPRNALTEEVKSVFSRAFTRWEEVTP-LTFTRVERFSTSDISIGFYSGEHGDG--EP 233
Query: 232 AVGFTFIVLDSTNVKSGFITLDATKYWALPTE--------HRGFDLETAAMHQIGHLLGL 283
G + + + +G LD + W + E DLE+ A+H+IGHLLGL
Sbjct: 234 FDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGL 293
Query: 284 EHSSDNKSIMYPTILPSHQKNVQITDSDNLAIQKLYSSSTKANAN 328
HSS SIMYPTI +K V +T D +Q LY ++ N +
Sbjct: 294 GHSSVEGSIMYPTIRTGRRK-VDLTTDDVEGVQYLYGANPNFNGS 337
>At1g59970 predicted GPI-anchored protein
Length = 360
Score = 127 bits (318), Expect = 1e-29
Identities = 97/308 (31%), Positives = 149/308 (47%), Gaps = 35/308 (11%)
Query: 81 GVDQIKQYLSDFGYLEQSGPFNNTLDQETVLALKTYQRYFNIQQDTL--SEILQHIALPR 138
G+ ++KQY FGY+ +G + D A+ TYQ+ FN++ S L+ I PR
Sbjct: 59 GLSKLKQYFRRFGYITTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPR 118
Query: 139 CGVPD-----------RILKYNLTNDISFPKGNQWFPKGTKNLTYGFDPRNKIPLDMTNV 187
CG PD +IL+ T SF G +PK ++LTY F P+N + ++ V
Sbjct: 119 CGNPDLIDGVSEMNGGKILR--TTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRV 176
Query: 188 FRTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDDDGIN-DVAVGFTFIVLDSTNVK 246
F A T+W+ T LNFT ++S A+I IGF++ DG D A+G + +++
Sbjct: 177 FSRAFTRWAEVTP-LNFTRSESILRADIVIGFFSGEHGDGEPFDGAMG---TLAHASSPP 232
Query: 247 SGFITLDATKYWALPTEHRG---------FDLETAAMHQIGHLLGLEHSSDNKSIMYPTI 297
+G + LD + W + DLE+ A+H+IGHLLGL HSS +IM+P I
Sbjct: 233 TGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAI 292
Query: 298 LPSHQKNVQITDSDNLAIQKLYSSSTKANANSDDSSGCFKLFGSSS-----SLLISLSIV 352
+K V++ D IQ LY + + S + G S +ISLS +
Sbjct: 293 SGGDRK-VELAKDDIEGIQHLYGGNPNGDGGGSKPSRESQSTGGDSVRRWRGWMISLSSI 351
Query: 353 FAFVALLN 360
+ L++
Sbjct: 352 ATCIFLIS 359
>At2g45040 metalloproteinase like protein ,predicted GPI-anchored
protein (by homology)
Length = 342
Score = 120 bits (301), Expect = 1e-27
Identities = 86/267 (32%), Positives = 133/267 (49%), Gaps = 30/267 (11%)
Query: 82 VDQIKQYLSDFGYLEQSGPFNNTLDQETVLALKTYQRYFNI------QQDTLSEILQHIA 135
+ +IK++L +GYL Q+ ++ ++ AL YQ+ + DTLS+IL
Sbjct: 50 IPEIKRHLQQYGYLPQNKESDDVSFEQ---ALVRYQKNLGLPITGKPDSDTLSQIL---- 102
Query: 136 LPRCGVPDRILK-----YNLTNDISFPKGNQWFPKGTKNLTYGFDPRNKIPL----DMTN 186
LPRCG PD + + + FP +W LTY F N P D+
Sbjct: 103 LPRCGFPDDVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRR 162
Query: 187 VFRTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDDDGINDVAVGFTFIVLDSTNVK 246
VFR A +W++ V +F ET+ Y A+IKIGF+N D G + G ++ + + +
Sbjct: 163 VFRRAFGKWASVIPV-SFIETEDYVIADIKIGFFN--GDHGDGEPFDGVLGVLAHTFSPE 219
Query: 247 SGFITLDATKYWALPTEHR----GFDLETAAMHQIGHLLGLEHSSDNKSIMYPTILPSHQ 302
+G + LD + WA+ + DLE+ A+H+IGH+LGL HSS + MYPT+ P
Sbjct: 220 NGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKP-RS 278
Query: 303 KNVQITDSDNLAIQKLYSSSTKANANS 329
K V + D + +Q LY ++ NS
Sbjct: 279 KKVNLNMDDVVGVQSLYGTNPNFTLNS 305
>At4g16640 proteinase-like protein, predicted GPI-anchored protein
(by homology)
Length = 364
Score = 114 bits (284), Expect = 1e-25
Identities = 87/268 (32%), Positives = 133/268 (49%), Gaps = 27/268 (10%)
Query: 81 GVDQIKQYLSDFGYLEQSGP-FNNTLDQETVLALKTYQRYFNI----QQDTLSEILQHIA 135
GV ++K+YL FGY+ F++ D A+ YQ + + DT + L ++
Sbjct: 69 GVSELKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTL--MS 126
Query: 136 LPRCGVPDRILKYNLTNDISFPKGNQWF----PKGTKN-LTYGFDPRNKIPL----DMTN 186
LPRCGV D + N ND + + PK ++ LTY +K+ D+
Sbjct: 127 LPRCGVSDTHMTIN--NDFLHTTAHYTYFNGKPKWNRDTLTYAISKTHKLDYLTSEDVKT 184
Query: 187 VFRTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDDDGINDVAVGFTFIVLDSTNVK 246
VFR A +QWS+ V +F E + A++KIGFY DG+ V T + + +
Sbjct: 185 VFRRAFSQWSSVIPV-SFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGT--LAHAFAPE 241
Query: 247 SGFITLDATKYWALPTEHRG-----FDLETAAMHQIGHLLGLEHSSDNKSIMYPTILPSH 301
+G + LDA + W + + +G DLE+ A H+IGHLLGL HSS ++MYP++ P
Sbjct: 242 NGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRP-R 300
Query: 302 QKNVQITDSDNLAIQKLYSSSTKANANS 329
K V +T D + KLY + K +S
Sbjct: 301 TKKVDLTVDDVAGVLKLYGPNPKLRLDS 328
>At3g49220 pectinesterase - like protein
Length = 598
Score = 32.0 bits (71), Expect = 0.54
Identities = 24/95 (25%), Positives = 41/95 (42%), Gaps = 13/95 (13%)
Query: 196 SNTTRVLNFTETKSYDDANIKIGFYNIT-----DDDGINDVAVG-------FTFIVLDST 243
++T R++ + + Y++ N+K+G I D G ++ G TF
Sbjct: 308 NSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFA 367
Query: 244 NVKSGFITLDAT-KYWALPTEHRGFDLETAAMHQI 277
+GFI D T + WA P +H+ L A H +
Sbjct: 368 ATGAGFIARDITFENWAGPAKHQAVALRIGADHAV 402
>At4g24350 putative protein
Length = 232
Score = 30.4 bits (67), Expect = 1.6
Identities = 27/95 (28%), Positives = 41/95 (42%), Gaps = 15/95 (15%)
Query: 180 IPLDMTNVFRTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDDDGINDV-AVGFTFI 238
IP +TN L W N +V D A + +G YN+ DG N++ ++G+ +
Sbjct: 141 IPKQITNA---GLWDWLNPDKVKGVE-----DIAYLDVGNYNVPKGDGDNELGSIGYNYE 192
Query: 239 VLDSTNVKSGFITLDATKYWALPTE---HRGFDLE 270
L S +G I +W T+ H DLE
Sbjct: 193 QLYSV---TGHINAPQNVFWINTTQEWLHLAADLE 224
>At1g50680 hypothetical protein
Length = 337
Score = 30.4 bits (67), Expect = 1.6
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 5/55 (9%)
Query: 287 SDNKSIMYPTI-LPSHQKNVQITDSDNLAIQKLYSSSTKANANSDDSSGCFKLFG 340
SDN S++ + + H +VQ+ ++NL L TK S+++ G F LFG
Sbjct: 281 SDNGSVVAEEVSMTVHDSSVQVKKTENLVSSMLEDKETK----SEENKGGFMLFG 331
>At1g70650 hypothetical protein
Length = 421
Score = 29.6 bits (65), Expect = 2.7
Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 1/65 (1%)
Query: 166 GTKNLTYGFDPRNKIPLDMTNVFRTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDD 225
G N++ + R + L+MT R+ T+ N TE+K YD ++ NI+DD
Sbjct: 348 GRSNVSKSAEKREQWKLEMTQRIRSNGTKAKKDDNTKNETESKCYDRRRNEL-LGNISDD 406
Query: 226 DGIND 230
++D
Sbjct: 407 GEMDD 411
>At3g06020 hypothetical protein
Length = 300
Score = 28.5 bits (62), Expect = 5.9
Identities = 19/52 (36%), Positives = 26/52 (49%), Gaps = 3/52 (5%)
Query: 296 TILPSHQKNVQITDSDN---LAIQKLYSSSTKANANSDDSSGCFKLFGSSSS 344
T L SH +N I DN + L SSS ++NSD +SG + S S+
Sbjct: 34 TPLKSHFQNSSIAPQDNPITINAASLPSSSPNPSSNSDTNSGSWSFLESLSN 85
>At5g14160 putative protein
Length = 352
Score = 28.1 bits (61), Expect = 7.7
Identities = 26/80 (32%), Positives = 40/80 (49%), Gaps = 8/80 (10%)
Query: 283 LEHSSDNKSIM----YPTILPSHQKNVQITDSDNLAIQKLYSSSTKANANSDDSSGCFKL 338
++ SSD + I Y + LPS QK+V + + + +Q+L SSS +S S L
Sbjct: 277 VQASSDIQGITRNSTYFSGLPSSQKDVFVFNLSSQKVQRLSSSS----ISSRPFSNARWL 332
Query: 339 FGSSSSLLISLSIVFAFVAL 358
F +S +I +F FV L
Sbjct: 333 FPTSYFTMILFPNIFVFVQL 352
>At3g43540 unknown protein
Length = 373
Score = 28.1 bits (61), Expect = 7.7
Identities = 18/43 (41%), Positives = 25/43 (57%), Gaps = 2/43 (4%)
Query: 85 IKQYLSDFGYLEQSGPFNNTLDQETVLALKTYQRYFNIQQDTL 127
+K+ L+ GYL S P+N T D E A + Y+R FN DT+
Sbjct: 85 LKELLAKEGYLIVSVPYNVTFDHEQA-AKQVYER-FNSCLDTI 125
>At2g22980 putative serine carboxypeptidase I
Length = 430
Score = 28.1 bits (61), Expect = 7.7
Identities = 33/124 (26%), Positives = 54/124 (42%), Gaps = 24/124 (19%)
Query: 133 HIALPRCGV--PDRIL-KYNLTN----------DISFPKGN--QWFPKGTKNLTYGFDPR 177
HI LP C + PD L +Y L + KG+ +W KN++Y +D +
Sbjct: 272 HILLPDCDITSPDCFLYRYTLITFWANNKSVREALQVNKGSIGKWVQCNYKNISYNYDIK 331
Query: 178 NKIPLDMTNVF--RTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDDDG---INDVA 232
+ + M N +L + ++ F T+++ I+ Y+ITDD IND
Sbjct: 332 SSVAYHMKNSIDGYRSLIYNGDHDMMVPFLATQAW----IRSLNYSITDDWKPWMINDQI 387
Query: 233 VGFT 236
G+T
Sbjct: 388 AGYT 391
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.135 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,054,129
Number of Sequences: 26719
Number of extensions: 353921
Number of successful extensions: 1030
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1002
Number of HSP's gapped (non-prelim): 15
length of query: 360
length of database: 11,318,596
effective HSP length: 101
effective length of query: 259
effective length of database: 8,619,977
effective search space: 2232574043
effective search space used: 2232574043
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144345.6