BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144345.5 - phase: 0 
         (131 letters)

Database: ara_mips 
           26,719 sequences; 11,318,596 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

At1g70170 predicted GPI-anchored protein                               75  1e-14
At4g16640 proteinase-like protein, predicted GPI-anchored protei...    73  4e-14
At2g45040 metalloproteinase like protein ,predicted GPI-anchored...    71  1e-13
At1g59970 predicted GPI-anchored protein                               66  6e-12
At1g24140 metalloproteinase like protein, predicted GPI-anchor         59  5e-10
At1g14740 unknown protein                                              26  5.1
At2g14660 hypothetical protein                                         26  6.6
At3g27930 unknown protein                                              25  8.7

>At1g70170 predicted GPI-anchored protein
          Length = 378

 Score = 75.1 bits (183), Expect = 1e-14
 Identities = 48/119 (40%), Positives = 67/119 (55%), Gaps = 4/119 (3%)

Query: 3   NVFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINYIDGVD-DVVVGDTVIKLGSNV 61
           +VF  AF RWS  T  LNF+ + S+  ++I IGFY  ++ DG   D V+G   +    + 
Sbjct: 187 SVFSRAFGRWSDVT-ALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLG--TLAHAFSP 243

Query: 62  NSGFIRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAI 120
            SG   L A + WV+  D   +       DLE+ A+H+IGHLLGL HS  +E +MYP I
Sbjct: 244 PSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTI 302


>At4g16640 proteinase-like protein, predicted GPI-anchored protein
           (by homology)
          Length = 364

 Score = 73.2 bits (178), Expect = 4e-14
 Identities = 46/129 (35%), Positives = 71/129 (54%), Gaps = 6/129 (4%)

Query: 4   VFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINYIDGVD-DVVVGDTVIKLGSNVN 62
           VFR AF++WS    V +F E   +  A++KIGFY  ++ DG+  D V+G           
Sbjct: 185 VFRRAFSQWSSVIPV-SFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPE-- 241

Query: 63  SGFIRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAILP 122
           +G + L A++ W++  D  +        DLE+ A H+IGHLLGL HS  +  VMYP++ P
Sbjct: 242 NGRLHLDAAETWIVDDD--LKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRP 299

Query: 123 LQQQRKLQI 131
             ++  L +
Sbjct: 300 RTKKVDLTV 308


>At2g45040 metalloproteinase like protein ,predicted GPI-anchored
           protein (by homology)
          Length = 342

 Score = 71.2 bits (173), Expect = 1e-13
 Identities = 48/129 (37%), Positives = 72/129 (55%), Gaps = 7/129 (5%)

Query: 4   VFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINYIDGVD-DVVVGDTVIKLGSNVN 62
           VFR AF +W+    V +F ET  Y  A+IKIGF+N ++ DG   D V+G  V+    +  
Sbjct: 163 VFRRAFGKWASVIPV-SFIETEDYVIADIKIGFFNGDHGDGEPFDGVLG--VLAHTFSPE 219

Query: 63  SGFIRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAILP 122
           +G + L  ++ W +  D    S      DLE+ A+H+IGH+LGL HS  K+  MYP + P
Sbjct: 220 NGRLHLDKAETWAVDFDEEKSSVA---VDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKP 276

Query: 123 LQQQRKLQI 131
             ++  L +
Sbjct: 277 RSKKVNLNM 285


>At1g59970 predicted GPI-anchored protein
          Length = 360

 Score = 65.9 bits (159), Expect = 6e-12
 Identities = 46/129 (35%), Positives = 69/129 (52%), Gaps = 24/129 (18%)

Query: 4   VFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINYIDGVD-DVVVGDTVIKLGSNVN 62
           VF  AFTRW++ T  LNF+ + S   A+I IGF++  + DG   D  +G   +   S+  
Sbjct: 176 VFSRAFTRWAEVTP-LNFTRSESILRADIVIGFFSGEHGDGEPFDGAMG--TLAHASSPP 232

Query: 63  SGFIRLIASKYWVLPTDNYMWSWQNGEF-----------DLETAAMHQIGHLLGLDHSFD 111
           +G + L   + W++          NGE            DLE+ A+H+IGHLLGL HS  
Sbjct: 233 TGMLHLDGDEDWLI---------SNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSV 283

Query: 112 KEYVMYPAI 120
           ++ +M+PAI
Sbjct: 284 EDAIMFPAI 292


>At1g24140 metalloproteinase like protein, predicted GPI-anchor
          Length = 384

 Score = 59.3 bits (142), Expect = 5e-10
 Identities = 41/119 (34%), Positives = 60/119 (49%), Gaps = 3/119 (2%)

Query: 3   NVFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINYIDGVDDVVVGDTVIKLGSNVN 62
           +VF  AFTRW + T  L F+    +  ++I IGFY+  + DG        T+    S   
Sbjct: 191 SVFSRAFTRWEEVTP-LTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPP- 248

Query: 63  SGFIRLIASKYWVLPTDNYMWSWQNGE-FDLETAAMHQIGHLLGLDHSFDKEYVMYPAI 120
           +G   L   + W++  +         E  DLE+ A+H+IGHLLGL HS  +  +MYP I
Sbjct: 249 TGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTI 307


>At1g14740 unknown protein
          Length = 733

 Score = 26.2 bits (56), Expect = 5.1
 Identities = 10/22 (45%), Positives = 16/22 (72%)

Query: 18  VLNFSETTSYDDANIKIGFYNI 39
           V+ FSE +SYDD  ++  F+N+
Sbjct: 93  VVTFSENSSYDDKWVERDFFNL 114


>At2g14660 hypothetical protein
          Length = 158

 Score = 25.8 bits (55), Expect = 6.6
 Identities = 8/18 (44%), Positives = 12/18 (66%)

Query: 72 KYWVLPTDNYMWSWQNGE 89
          +YW+L T+   WSW + E
Sbjct: 6  RYWLLKTEPNEWSWSDQE 23


>At3g27930 unknown protein
          Length = 425

 Score = 25.4 bits (54), Expect = 8.7
 Identities = 10/29 (34%), Positives = 16/29 (54%)

Query: 8   AFTRWSQTTRVLNFSETTSYDDANIKIGF 36
           AF  W + +   N S TT++   N++ GF
Sbjct: 331 AFKSWWKPSFAFNISATTNHRTGNVQCGF 359


  Database: ara_mips
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,978,382
  Number of sequences in database:  6832
  
  Database: /data/blast2/ara_mips_chr2
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,737,135
  Number of sequences in database:  4184
  
  Database: /data/blast2/ara_mips_chr3
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,236,886
  Number of sequences in database:  5377
  
  Database: /data/blast2/ara_mips_chr4
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,748,816
  Number of sequences in database:  4030
  
  Database: /data/blast2/ara_mips_chr5
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,569,679
  Number of sequences in database:  6098
  
  Database: /data/blast2/ara_mips_chl
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 25,951
  Number of sequences in database:  85
  
  Database: /data/blast2/ara_mips_mit
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 21,747
  Number of sequences in database:  113
  
Lambda     K      H
   0.322    0.138    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,055,731
Number of Sequences: 26719
Number of extensions: 123958
Number of successful extensions: 245
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 229
Number of HSP's gapped (non-prelim): 8
length of query: 131
length of database: 11,318,596
effective HSP length: 88
effective length of query: 43
effective length of database: 8,967,324
effective search space: 385594932
effective search space used: 385594932
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)