BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144345.13 + phase: 0
(471 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17930 unknown protein 37 0.024
At1g05320 unknown protein 35 0.089
At5g48160 putative protein 35 0.12
At3g07780 unknown protein 32 0.58
At1g57690 32 0.99
At5g63480 unknown protein 31 1.7
At1g09470 hypothetical protein 31 1.7
At1g10890 unknown protein 30 2.2
At3g44630 putative disease resistance protein 30 2.9
At2g27170 putative chromosome associated protein 30 2.9
At5g41140 putative protein 30 3.8
At4g21670 putative protein 29 4.9
At3g53840 protein kinase-like protein 29 4.9
At3g48860 putative protein 29 4.9
At2g39380 hypothetical protein 29 4.9
At3g48520 cytochrome P450-like protein 29 6.4
At5g52430 putative protein 28 8.4
At4g12300 flavonoid 3',5'-hydroxylase -like protein 28 8.4
At2g30400 unknown protein 28 8.4
>At1g17930 unknown protein
Length = 478
Score = 37.0 bits (84), Expect = 0.024
Identities = 58/223 (26%), Positives = 94/223 (42%), Gaps = 21/223 (9%)
Query: 46 NIEKGCLETLVQFYDPAYHCFTFPDYQLMPTLEEYSHLIGLPVLDKVPFTGLEPFPKAAT 105
++ + LV+ + + F FP ++ TL+E S ++GL V D P G++ + +
Sbjct: 61 SLNNSLISALVERWRRETNTFHFPCGEMTITLDEVSLILGLAV-DGKPVVGVKEKDEDPS 119
Query: 106 IANALHLKTSLIKEKLTLKGNLPSLPTKFLYQQAFNFSKTNNV-EAFYSILALLIY--GL 162
L L L K +L+ GN + K+L + K + E Y A LIY G
Sbjct: 120 QV-CLRLLGKLPKGELS--GN--RVTAKWLKESFAECPKGATMKEIEYHTRAYLIYIVGS 174
Query: 163 VLFPNID-NYVDIHAIQIFLTKNPV------PTLLADIYHSIHDRTQVGRGAILGCAPLL 215
+F D + + + + +F LA +Y I + +Q + I GC LL
Sbjct: 175 TIFATTDPSKISVDYLILFEDFEKAGEYAWGAAALAFLYRQIGNASQRSQSIIGGCLTLL 234
Query: 216 YKWFTSHL----PQTHSFQANPENLSWPKRIMSLTPSDITWYR 254
W HL P+ + Q P L W R S + +D+ YR
Sbjct: 235 QCWSYFHLNIDRPKRTTRQF-PLALLWKGRQQSRSKNDLFKYR 276
>At1g05320 unknown protein
Length = 841
Score = 35.0 bits (79), Expect = 0.089
Identities = 19/67 (28%), Positives = 35/67 (51%)
Query: 386 VEECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQEHEVHKLRRQIVKKNVQIRAQ 445
+EE + ++ ++ V T K + +EA+LK+ T+ IE+ E E L +K N ++ Q
Sbjct: 455 IEEHKRQVLEASGVADTRKVEVEEALLKLNTLESTIEELEKENGDLAEVNIKLNQKLANQ 514
Query: 446 STRLSQF 452
+ F
Sbjct: 515 GSETDDF 521
>At5g48160 putative protein
Length = 574
Score = 34.7 bits (78), Expect = 0.12
Identities = 19/56 (33%), Positives = 30/56 (52%)
Query: 387 EECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQEHEVHKLRRQIVKKNVQI 442
E QE L + E V R + +A + +ET E+E + EV +L+ + KK +QI
Sbjct: 395 EVVQETLRKMEIVAEEKMRMFKKARMALETCDRELEDKAKEVSELKAERQKKKLQI 450
>At3g07780 unknown protein
Length = 566
Score = 32.3 bits (72), Expect = 0.58
Identities = 18/56 (32%), Positives = 30/56 (53%)
Query: 387 EECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQEHEVHKLRRQIVKKNVQI 442
E +E L + E VG R Y +A + +E E+E++ +V +L+ + KK QI
Sbjct: 393 EVVKETLRKMEIVGEEKTRMYKKARMGLEECEREVEEKAKQVAELQMERQKKKQQI 448
>At1g57690
Length = 330
Score = 31.6 bits (70), Expect = 0.99
Identities = 37/138 (26%), Positives = 53/138 (37%), Gaps = 22/138 (15%)
Query: 11 FRKLDLENLKKLAFEVTNLENFCDRHGKLL--GVLWTNIEK-----------------GC 51
FR + ++ V +L + D H + GV WT+++K GC
Sbjct: 114 FRSNKIPRFYEINSSVKSLSHRLDLHDVIPRHGVSWTSLKKFSLSYCGLPDESAKILSGC 173
Query: 52 --LETLVQFYDPAYHCFTFPDYQLMPTLEEYSHLIGLPVLDKVPFTGLEPFPKAATIANA 109
LE +Q P HC + QL TL + S L VLD + F + A
Sbjct: 174 PILEWPMQIVAPQIHCLKLRNTQLPCTLVDVSSLTEAEVLDIIIFPVNLSYNADFLHATM 233
Query: 110 LHLKTSLIK-EKLTLKGN 126
L + L EKLT G+
Sbjct: 234 LEMLKKLKNVEKLTFSGS 251
>At5g63480 unknown protein
Length = 189
Score = 30.8 bits (68), Expect = 1.7
Identities = 14/41 (34%), Positives = 23/41 (55%)
Query: 420 EIEQQEHEVHKLRRQIVKKNVQIRAQSTRLSQFISAGERWE 460
EIE+ E + LR +I KKNV ++ +L + I+ W+
Sbjct: 144 EIEKLEEQALSLRMEIAKKNVHVKELIDKLRELIADISTWQ 184
>At1g09470 hypothetical protein
Length = 359
Score = 30.8 bits (68), Expect = 1.7
Identities = 19/68 (27%), Positives = 37/68 (53%), Gaps = 7/68 (10%)
Query: 386 VEECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQEHEVHKLRRQIVKKNVQIRAQ 445
++E + RLA+ E + EAM + E + +++ E E+H+L +++ +K QIRA
Sbjct: 35 LKEARTRLAEQE------RSCSKEAMSRQEAET-RVKRMEDEMHELAKELNEKVEQIRAS 87
Query: 446 STRLSQFI 453
+F+
Sbjct: 88 DVATEKFV 95
>At1g10890 unknown protein
Length = 592
Score = 30.4 bits (67), Expect = 2.2
Identities = 22/82 (26%), Positives = 36/82 (43%), Gaps = 8/82 (9%)
Query: 372 STTPAPALPLAPATVEECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQEHEVHKL 431
S+TP+PA AT+E + R + K K +E K E++ E E K
Sbjct: 38 SSTPSPAKRSPAATLESAKNRNGE--------KLKREEEERKRRQREAELKLIEEETVKR 89
Query: 432 RRQIVKKNVQIRAQSTRLSQFI 453
+ ++K V+ QS ++ I
Sbjct: 90 VEEAIRKKVEESLQSEKIKMEI 111
>At3g44630 putative disease resistance protein
Length = 1214
Score = 30.0 bits (66), Expect = 2.9
Identities = 17/46 (36%), Positives = 26/46 (55%)
Query: 74 MPTLEEYSHLIGLPVLDKVPFTGLEPFPKAATIANALHLKTSLIKE 119
+ TL +LI L +LD + L+ FP+ +T + L LK + IKE
Sbjct: 894 LETLPTNINLISLRILDLTDCSQLKSFPEISTHISELRLKGTAIKE 939
>At2g27170 putative chromosome associated protein
Length = 1163
Score = 30.0 bits (66), Expect = 2.9
Identities = 18/57 (31%), Positives = 31/57 (53%), Gaps = 2/57 (3%)
Query: 386 VEECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQEHEVHKLRRQI--VKKNV 440
+ E + R+++S + T KR+ DE K + GE Q E+ KL+ ++ KKN+
Sbjct: 441 IGELESRISKSHELFNTKKRERDEEQRKRKEKWGEESQLSSEIDKLKTELERAKKNL 497
>At5g41140 putative protein
Length = 983
Score = 29.6 bits (65), Expect = 3.8
Identities = 13/64 (20%), Positives = 30/64 (46%)
Query: 387 EECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQEHEVHKLRRQIVKKNVQIRAQS 446
EE Q + + E V K + + A+ + + + E E+ LR+Q+V+ ++ +
Sbjct: 763 EELQRIIDEKEAVITALKSQLETAIAPCDNLKHSLSNNESEIENLRKQVVQVRSELEKKE 822
Query: 447 TRLS 450
++
Sbjct: 823 EEMA 826
>At4g21670 putative protein
Length = 995
Score = 29.3 bits (64), Expect = 4.9
Identities = 13/41 (31%), Positives = 25/41 (60%)
Query: 418 SGEIEQQEHEVHKLRRQIVKKNVQIRAQSTRLSQFISAGER 458
S E+ QQ+ +V K R++ ++ +++ R+S F +GER
Sbjct: 24 SRELNQQQDDVMKQRKKKQREVMELAKMGIRISHFSQSGER 64
>At3g53840 protein kinase-like protein
Length = 640
Score = 29.3 bits (64), Expect = 4.9
Identities = 26/96 (27%), Positives = 40/96 (41%), Gaps = 20/96 (20%)
Query: 265 SCGEYSNVPLLG-TQGGISYNPILAKRQFGCPMEAKPDNIYLQGEFYFNHEDPSNKRGRF 323
SC +SN+PLLG GG+ I + G N+R
Sbjct: 277 SCKRWSNLPLLGGLAGGVGA-------------------ILIAGFITKTIVSKQNRRIAG 317
Query: 324 VQAWHAIRTLNRSQLARRSDSLQGSYTQWVINRASD 359
Q+W ++R L+R+ L+ S L +T I +A+D
Sbjct: 318 NQSWASVRKLHRNLLSINSTGLDRIFTGKEIVKATD 353
>At3g48860 putative protein
Length = 609
Score = 29.3 bits (64), Expect = 4.9
Identities = 27/92 (29%), Positives = 39/92 (42%), Gaps = 2/92 (2%)
Query: 367 PRYLSSTTPAPALPLAPATVEECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQEH 426
P +S TP PL P T + A V + K EA + + E+ E+
Sbjct: 171 PSRVSVKTPVSIPPLDPPTRSRDKRFFADVPSVNSKEKGDQREASALRDELDMLQEENEN 230
Query: 427 EVHKLRRQIVKKNVQIRAQSTRL-SQFISAGE 457
+ KLRR +K V+ A++ L Q S GE
Sbjct: 231 VLEKLRR-AEEKRVEAEARAKELEKQVASLGE 261
>At2g39380 hypothetical protein
Length = 637
Score = 29.3 bits (64), Expect = 4.9
Identities = 24/100 (24%), Positives = 39/100 (39%), Gaps = 13/100 (13%)
Query: 146 NNVEAFYSILALLIYGLVLFPNIDNYVDIHAIQIFLTKNPVPTLLADIYHSIHDRTQVGR 205
N+ + + +LI+ L ++Y D+ +FL N + D S H R +G
Sbjct: 429 NHAVSVHLAWLILIFLCKLDIKAESYKDVSLSYLFLVNNI--QFVVDTVRSTHLRNLLGD 486
Query: 206 GAILGCAPLLYKWFTSHLPQTHSFQANPENLSWPKRIMSL 245
W T H + S+ AN E +W +SL
Sbjct: 487 D-----------WLTKHEAKLRSYAANYEIAAWANVYISL 515
>At3g48520 cytochrome P450-like protein
Length = 506
Score = 28.9 bits (63), Expect = 6.4
Identities = 33/107 (30%), Positives = 49/107 (44%), Gaps = 16/107 (14%)
Query: 367 PRYLSSTTPAPALPLAPATVEECQERLAQSECVGATWKRKYDEAMLKMETMSGEIEQQE- 425
P L T P L A T E R A +E + A WK K ++ + E + +E
Sbjct: 196 PDCLDLTRPVNPLVEAFDTAAEISARRA-TEPIYAVWKTK------RVLNVGSERKLREA 248
Query: 426 -HEVHKLRRQIV---KKNVQI----RAQSTRLSQFISAGERWEFFKD 464
VH L +IV KK+++I A+ LS+F++AG E +D
Sbjct: 249 IRTVHVLVSEIVRAKKKSLEIGTGAEAKQDLLSRFLAAGHNGEAVRD 295
>At5g52430 putative protein
Length = 438
Score = 28.5 bits (62), Expect = 8.4
Identities = 14/55 (25%), Positives = 25/55 (45%), Gaps = 1/55 (1%)
Query: 414 METMSGEIEQQEHEVHKLRRQIVKKNVQIRAQSTRLSQFIS-AGERWEFFKDAHS 467
+E SG+ E ++H + KL + + + + +T+ AG W FF S
Sbjct: 381 IEKRSGDRENEQHRIQKLSSSSIGSSKEFKFDNTKDENIEKVAGNSWSFFPGLRS 435
>At4g12300 flavonoid 3',5'-hydroxylase -like protein
Length = 516
Score = 28.5 bits (62), Expect = 8.4
Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
Query: 85 GLPVLDKVPFTGLEPFPKAATIANALHLKTSL-IKEKLTLKGNLPSLPTKFLYQQAFNFS 143
GLP++ +PF + A +A + L + KLT+ N PSL + L Q NFS
Sbjct: 48 GLPIVGNLPFLDPDLHTYFANLAQSHGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFS 107
>At2g30400 unknown protein
Length = 320
Score = 28.5 bits (62), Expect = 8.4
Identities = 18/48 (37%), Positives = 26/48 (53%), Gaps = 2/48 (4%)
Query: 108 NALHLKTSLIKEKLTLKGNL-PSLPTKFLYQQAFNFSKTNNVEAFYSI 154
N+LH K + K K K +L P P L +FN SK N+ ++ YS+
Sbjct: 74 NSLHTK-KMSKRKTLYKPSLKPLTPPPLLVSASFNKSKINDQDSSYSL 120
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.136 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,929,540
Number of Sequences: 26719
Number of extensions: 483643
Number of successful extensions: 1254
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 1243
Number of HSP's gapped (non-prelim): 21
length of query: 471
length of database: 11,318,596
effective HSP length: 103
effective length of query: 368
effective length of database: 8,566,539
effective search space: 3152486352
effective search space used: 3152486352
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC144345.13