BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142498.2 + phase: 0 /pseudo
(193 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55980 unknown protein 205 1e-53
At2g40140 putative CCCH-type zinc finger protein 196 6e-51
At5g58620 unknown protein 183 6e-47
At5g12850 zinc finger transcription factor -like protein 159 7e-40
At2g41900 CCCH-type zinc finger like protein 150 5e-37
At5g07500 zinc finger transcription factor 68 3e-12
At2g19810 putative CCCH-type zinc finger protein 63 8e-11
At2g25900 Cys3His zinc finger like protein ATCTH (ATCTH) 62 2e-10
At4g29190 unknown protein 60 5e-10
At5g44260 zinc finger like protein 59 1e-09
At1g03790 unknown protein 57 6e-09
At5g12320 unknown protein 39 0.001
At5g61230 unknown protein 37 0.005
At4g19150 ankyrin-like protein 35 0.032
At3g04710 ankyrin-like protein 35 0.032
At2g25600 potassium transporter/channel like protein 33 0.094
At2g03430 ankyrin like protein 33 0.12
At3g24530 unknown protein 32 0.16
At2g01680 unknown protein 32 0.16
At1g14000 putative protein kinase 32 0.16
>At3g55980 unknown protein
Length = 580
Score = 205 bits (521), Expect = 1e-53
Identities = 107/210 (50%), Positives = 136/210 (63%), Gaps = 28/210 (13%)
Query: 10 LLELSAIDDIEAFKREVEEKGYDVNEAGFWYCRKIGSKKMCYEKRTPLMIASLFGSIRVV 69
LLE +A DD+++FKREVEEKG D++E+G WYCR++GSKKM E+RTPLM+A+++GSI+V+
Sbjct: 30 LLEFAACDDLDSFKREVEEKGLDLDESGLWYCRRVGSKKMGLEERTPLMVAAMYGSIKVL 89
Query: 70 KYIIETNMVNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLDAGADVDF--------LD 121
+I+ T +VN A G E VT LHCAVAG S + E++ +LLDA A V+ LD
Sbjct: 90 TFIVSTGKSDVNRACGEERVTPLHCAVAGCSVNMIEVINVLLDASALVNSVDANGNQPLD 149
Query: 122 EVVRQKLSVANSKELVAE--------------------KKDSGYAIDTSLPDINNGVFVT 161
VR VA+ + E K S Y D SLPDIN GV+ +
Sbjct: 150 VFVRVSRFVASPRRKAVELLLRGGGVGGLIDEAVEEEIKIVSKYPADASLPDINEGVYGS 209
Query: 162 DEFRMYSFKVKTCSRGYTHDWTECPFVHPG 191
DEFRMYSFKVK CSR Y+HDWTEC FVHPG
Sbjct: 210 DEFRMYSFKVKPCSRAYSHDWTECAFVHPG 239
>At2g40140 putative CCCH-type zinc finger protein
Length = 597
Score = 196 bits (498), Expect = 6e-51
Identities = 102/210 (48%), Positives = 135/210 (63%), Gaps = 28/210 (13%)
Query: 10 LLELSAIDDIEAFKREVEEK-GYDVNEAGFWYCRKIGSKKMCYEKRTPLMIASLFGSIRV 68
LLE +A DD+ +FKRE+EE +++E+GFWYCR++GSKKM +E+RTPLM+A+++GS+ V
Sbjct: 33 LLEFAACDDLSSFKREIEENPSVEIDESGFWYCRRVGSKKMGFEERTPLMVAAMYGSMEV 92
Query: 69 VKYIIETNMVNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLDAGADVDFLD------- 121
+ YII T +VN E VTALHCAV+G S S EI+K+LLDA A + +D
Sbjct: 93 LNYIIATGRSDVNRVCSDEKVTALHCAVSGCSVSIVEIIKILLDASASPNCVDANGNKPV 152
Query: 122 ------------------EVVRQKL--SVANSKELVAEKKDSGYAIDTSLPDINNGVFVT 161
EV+ + SV +E + + Y D SLPDIN GV+ T
Sbjct: 153 DLLAKDSRFVPNQSRKAVEVLLTGIHGSVMEEEEEELKSVVTKYPADASLPDINEGVYGT 212
Query: 162 DEFRMYSFKVKTCSRGYTHDWTECPFVHPG 191
D+FRM+SFKVK CSR Y+HDWTECPFVHPG
Sbjct: 213 DDFRMFSFKVKPCSRAYSHDWTECPFVHPG 242
>At5g58620 unknown protein
Length = 607
Score = 183 bits (464), Expect = 6e-47
Identities = 105/226 (46%), Positives = 136/226 (59%), Gaps = 42/226 (18%)
Query: 8 STLLELSAIDDIEAFKREVEEKGYD-VNEAGFWYCRKIGSKKMCYEKRTPLMIASLFGSI 66
S LLE SA +D+ FK VEE+G + ++ +G WY R++GSKKM +E+RTPLMIA+LFGS
Sbjct: 12 SLLLESSACNDLSGFKSLVEEEGLESIDGSGLWYGRRLGSKKMGFEERTPLMIAALFGSK 71
Query: 67 RVVKYIIETNMVNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLDAGADVDFLD----- 121
VV YII T +V+VN + GS+ TALHCAV+G S + EIV LLL A+ D D
Sbjct: 72 EVVDYIISTGLVDVNRSCGSDGATALHCAVSGLSANSLEIVTLLLKGSANPDSCDAYGNK 131
Query: 122 --EVVRQKLS-VANSKELVAEKKDSG---------------------------------Y 145
+V+ LS V +++ V E+ G Y
Sbjct: 132 PGDVIFPCLSPVFSARMKVLERLLKGNDDLNEVNGQEESEPEVEVEVEVSPPRGSERKEY 191
Query: 146 AIDTSLPDINNGVFVTDEFRMYSFKVKTCSRGYTHDWTECPFVHPG 191
+D +LPDI NGV+ TDEFRMY+FK+K CSR Y+HDWTECPFVHPG
Sbjct: 192 PVDPTLPDIKNGVYGTDEFRMYAFKIKPCSRAYSHDWTECPFVHPG 237
>At5g12850 zinc finger transcription factor -like protein
Length = 706
Score = 159 bits (403), Expect = 7e-40
Identities = 91/247 (36%), Positives = 132/247 (52%), Gaps = 64/247 (25%)
Query: 8 STLLELSAIDDIEAFKREVEEKGYDVNEAGFWYCRKIGSKKMCYEKRTPLMIASLFGSIR 67
S LLE +A +D+E F+R++ + +N+ G WY R+ ++M E+RTPLM+ASL+GS+
Sbjct: 40 SALLEFAADNDVEGFRRQLSDVSC-INQMGLWYRRQRFVRRMVLEQRTPLMVASLYGSLD 98
Query: 68 VVKYIIETNMVNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLDAGAD----------- 116
VVK+I+ +N++ G + TALHCA +G S + ++VKLLL GAD
Sbjct: 99 VVKFILSFPEAELNLSCGPDKSTALHCAASGASVNSLDVVKLLLSVGADPNIPDAHGNRP 158
Query: 117 VDFL-------------------DEVVRQKL-----SVANSKELVAEKKDSG-------- 144
VD L DE++ + L S+ +S ++ D+G
Sbjct: 159 VDVLVVSPHAPGLRTILEEILKKDEIISEDLHASSSSLGSSFRSLSSSPDNGSSLLSLDS 218
Query: 145 --------------------YAIDTSLPDINNGVFVTDEFRMYSFKVKTCSRGYTHDWTE 184
Y ID SLPDI +G++ TDEFRM+SFK++ CSR Y+HDWTE
Sbjct: 219 VSSPTKPHGTDVTFASEKKEYPIDPSLPDIKSGIYSTDEFRMFSFKIRPCSRAYSHDWTE 278
Query: 185 CPFVHPG 191
CPF HPG
Sbjct: 279 CPFAHPG 285
>At2g41900 CCCH-type zinc finger like protein
Length = 716
Score = 150 bits (378), Expect = 5e-37
Identities = 91/244 (37%), Positives = 118/244 (48%), Gaps = 60/244 (24%)
Query: 8 STLLELSAIDDIEAFKREVEEKGYDVNEAGFWYCRKIGSKKMCYEKRTPLMIASLFGSIR 67
++LLEL+A +D+E + +E V+EAG WY R+ GSK M + RTPLM+A+ +GSI
Sbjct: 46 ASLLELAANNDVEGVRLSIERDPSCVDEAGLWYGRQKGSKAMVNDYRTPLMVAATYGSID 105
Query: 68 VVKYIIETNMVNVNMAIGSENVTALHCAVAGGSKS------------------------- 102
V+K I+ +VN A G++ TALHCA +GG+ +
Sbjct: 106 VIKLIVSLTDADVNRACGNDQTTALHCAASGGAVNAIQVVKLLLAAGADLNLLDAEGQRA 165
Query: 103 --------KFEIVKLLLDAGADVDFLDEVVRQKLSVANSKEL--------VAEKKDSG-- 144
K E VKL+L D R V N E SG
Sbjct: 166 GDVIVVPPKLEGVKLMLQELLSADGSSTAERNLRVVTNVPNRSSSPCHSPTGENGGSGSG 225
Query: 145 -----------------YAIDTSLPDINNGVFVTDEFRMYSFKVKTCSRGYTHDWTECPF 187
Y +D SLPDI N ++ TDEFRMYSFKV+ CSR Y+HDWTECPF
Sbjct: 226 SPLGSPFKLKSTEFKKEYPVDPSLPDIKNSIYATDEFRMYSFKVRPCSRAYSHDWTECPF 285
Query: 188 VHPG 191
VHPG
Sbjct: 286 VHPG 289
>At5g07500 zinc finger transcription factor
Length = 245
Score = 68.2 bits (165), Expect = 3e-12
Identities = 25/47 (53%), Positives = 37/47 (78%)
Query: 145 YAIDTSLPDINNGVFVTDEFRMYSFKVKTCSRGYTHDWTECPFVHPG 191
Y ID S+P+I++ ++ +DEFRMY++K+K C R +HDWTECP+ H G
Sbjct: 34 YEIDPSIPNIDDAIYGSDEFRMYAYKIKRCPRTRSHDWTECPYAHRG 80
>At2g19810 putative CCCH-type zinc finger protein
Length = 359
Score = 63.2 bits (152), Expect = 8e-11
Identities = 25/44 (56%), Positives = 30/44 (67%)
Query: 148 DTSLPDINNGVFVTDEFRMYSFKVKTCSRGYTHDWTECPFVHPG 191
D S PD + D FRMY FKV+ C+RG +HDWTECP+ HPG
Sbjct: 63 DLSGPDSPIDAYTCDHFRMYEFKVRRCARGRSHDWTECPYAHPG 106
>At2g25900 Cys3His zinc finger like protein ATCTH (ATCTH)
Length = 315
Score = 61.6 bits (148), Expect = 2e-10
Identities = 22/33 (66%), Positives = 28/33 (84%)
Query: 159 FVTDEFRMYSFKVKTCSRGYTHDWTECPFVHPG 191
F +DEFR+Y FK++ C+RG +HDWTECPF HPG
Sbjct: 86 FSSDEFRIYEFKIRRCARGRSHDWTECPFAHPG 118
>At4g29190 unknown protein
Length = 356
Score = 60.5 bits (145), Expect = 5e-10
Identities = 23/40 (57%), Positives = 28/40 (69%)
Query: 152 PDINNGVFVTDEFRMYSFKVKTCSRGYTHDWTECPFVHPG 191
PD + D FRMY FKV+ C+RG +HDWTECP+ HPG
Sbjct: 68 PDSPIDAYSCDHFRMYDFKVRRCARGRSHDWTECPYAHPG 107
>At5g44260 zinc finger like protein
Length = 381
Score = 59.3 bits (142), Expect = 1e-09
Identities = 31/74 (41%), Positives = 41/74 (54%), Gaps = 13/74 (17%)
Query: 125 RQKLSVANSKELVA-------EKKDSGYAIDTSLPDINNGVFVTDEFRMYSFKVKTCSRG 177
R+ LS A S V+ E+KD Y D+ P + D FRMY FK++ C+R
Sbjct: 21 RKLLSSAKSPSSVSSPLRDYKEQKDYCYDSDSEDP------YAGDHFRMYEFKIRRCTRS 74
Query: 178 YTHDWTECPFVHPG 191
+HDWT+CPF HPG
Sbjct: 75 RSHDWTDCPFSHPG 88
>At1g03790 unknown protein
Length = 393
Score = 57.0 bits (136), Expect = 6e-09
Identities = 23/53 (43%), Positives = 33/53 (61%), Gaps = 6/53 (11%)
Query: 139 EKKDSGYAIDTSLPDINNGVFVTDEFRMYSFKVKTCSRGYTHDWTECPFVHPG 191
E K+ Y D+ P + +D FRM+ FK++ C+R +HDWT+CPF HPG
Sbjct: 64 ENKEYCYDSDSDDP------YASDHFRMFEFKIRRCTRSRSHDWTDCPFAHPG 110
>At5g12320 unknown protein
Length = 144
Score = 39.3 bits (90), Expect = 0.001
Identities = 33/98 (33%), Positives = 52/98 (52%), Gaps = 9/98 (9%)
Query: 54 RTPLMIASLFGSIRVVKYIIETNMVNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLDA 113
RT L +A+ G + +V+Y+I V++N A+ EN LH A G E+VK L+ A
Sbjct: 45 RTALHMAAANGHMTIVEYLISEG-VDIN-ALNDENNAPLHWACLNGH---VEVVKRLILA 99
Query: 114 GADVDFLDEVVRQKLSVANSKELVAEKKDSGYAIDTSL 151
GA + L+ R + A + AEK + AI+T++
Sbjct: 100 GASLSLLNRYERTPMDEA----IGAEKMEIIDAINTTV 133
>At5g61230 unknown protein
Length = 174
Score = 37.4 bits (85), Expect = 0.005
Identities = 24/65 (36%), Positives = 38/65 (57%), Gaps = 5/65 (7%)
Query: 56 PLMIASLFGSIRVVKYIIETNMVNVNMAIG--SENVTALHCAVAGGSKSKFEIVKLLLDA 113
PL I + G ++ VK +++ M +A G S+ V+ALH A GG E++ LLL+
Sbjct: 32 PLHIKARKGDLKSVKQLLDQGMDVNALAWGPKSKGVSALHLAAEGGH---IEVMDLLLER 88
Query: 114 GADVD 118
GA++D
Sbjct: 89 GANID 93
>At4g19150 ankyrin-like protein
Length = 243
Score = 34.7 bits (78), Expect = 0.032
Identities = 24/64 (37%), Positives = 35/64 (54%), Gaps = 5/64 (7%)
Query: 54 RTPLMIASLFGSIRVVKYIIETNMVNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLDA 113
RTPL +A+ G VV Y+ + N +V A G +++ A+H A K E+V+ LL A
Sbjct: 51 RTPLHLAAWAGHNEVVSYLCK-NKADVGAAAG-DDMGAIHFA---SQKGHLEVVRTLLSA 105
Query: 114 GADV 117
G V
Sbjct: 106 GGSV 109
>At3g04710 ankyrin-like protein
Length = 456
Score = 34.7 bits (78), Expect = 0.032
Identities = 25/65 (38%), Positives = 35/65 (53%), Gaps = 7/65 (10%)
Query: 55 TPLMIASLFGSIRVVKYIIETNM-VNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLDA 113
TPL+ A+ G I VKY++E N+ +G+ TALH A G + E++K LL
Sbjct: 91 TPLVHAARQGQIETVKYLLEQGADPNIASELGA---TALHHAAGTG---EIELLKELLSR 144
Query: 114 GADVD 118
G VD
Sbjct: 145 GVPVD 149
Score = 33.1 bits (74), Expect = 0.094
Identities = 27/100 (27%), Positives = 44/100 (44%), Gaps = 7/100 (7%)
Query: 53 KRTPLMIASLFGSIRVVKYIIETNMVNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLD 112
KR L A+ G + +Y++E +N + + + +H A G + E VK LL+
Sbjct: 55 KRGALHFAAREGQTEICRYLLEELKLNADAKDETGDTPLVHAARQG----QIETVKYLLE 110
Query: 113 AGADVDFLDEVVRQKLSVA---NSKELVAEKKDSGYAIDT 149
GAD + E+ L A EL+ E G +D+
Sbjct: 111 QGADPNIASELGATALHHAAGTGEIELLKELLSRGVPVDS 150
Score = 29.6 bits (65), Expect = 1.0
Identities = 32/113 (28%), Positives = 46/113 (40%), Gaps = 31/113 (27%)
Query: 52 EKRTPLMIASLFGSIRVVKYIIETNMVNVNMAIGSENVTALHCAVAGGSKS--------- 102
E TPL+ A+ V+ ++E N N N A +N+T L AVA GS S
Sbjct: 153 ESGTPLIWAAGHDQKNAVEVLLEHN-ANPN-AETEDNITPLLSAVAAGSLSCLELLVKAG 210
Query: 103 --------------------KFEIVKLLLDAGADVDFLDEVVRQKLSVANSKE 135
E++ LL AGAD + DE + L VA +++
Sbjct: 211 AKANVFAGGATPLHIAADIGNLELINCLLKAGADPNQKDEEGNRPLEVAAARD 263
>At2g25600 potassium transporter/channel like protein
Length = 888
Score = 33.1 bits (74), Expect = 0.094
Identities = 19/32 (59%), Positives = 22/32 (68%), Gaps = 3/32 (9%)
Query: 90 TALHCAVAGGSKSKFEIVKLLLDAGADVDFLD 121
TALH AV+ G EIVK LLD GAD+D+ D
Sbjct: 676 TALHRAVSEGH---LEIVKFLLDQGADLDWPD 704
>At2g03430 ankyrin like protein
Length = 240
Score = 32.7 bits (73), Expect = 0.12
Identities = 25/68 (36%), Positives = 38/68 (55%), Gaps = 5/68 (7%)
Query: 56 PLMIASLFGSIRVVKYIIETNMVNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLDAGA 115
PL A+ G+ +V+ ++ T +VN A + TALH A SK + EI +LLL GA
Sbjct: 86 PLHSAASIGNAELVEVLL-TRGADVN-AKNNGGRTALHYAA---SKGRLEIAQLLLTHGA 140
Query: 116 DVDFLDEV 123
++ D+V
Sbjct: 141 KINITDKV 148
Score = 30.8 bits (68), Expect = 0.47
Identities = 26/82 (31%), Positives = 42/82 (50%), Gaps = 4/82 (4%)
Query: 54 RTPLMIASLFGSIRVVKYIIETNMVN-VNMAIGSENVTALHCAVAGGSKSKFEIVKLLLD 112
R+ L +A+ FG ++VK + ++ V + E LH A + G+ E+V++LL
Sbjct: 48 RSLLHVAASFGHSQIVKLLSSSDEAKTVINSKDDEGWAPLHSAASIGNA---ELVEVLLT 104
Query: 113 AGADVDFLDEVVRQKLSVANSK 134
GADV+ + R L A SK
Sbjct: 105 RGADVNAKNNGGRTALHYAASK 126
>At3g24530 unknown protein
Length = 481
Score = 32.3 bits (72), Expect = 0.16
Identities = 22/66 (33%), Positives = 37/66 (55%), Gaps = 5/66 (7%)
Query: 55 TPLMIASLFGSIRVVKYIIE-TNMVNVNM-AIGSENVTALHCAVAGGSKSKFEIVKLLLD 112
TPL +++ G++ +VKY++ T V + A+ + T LH A G E KLLL+
Sbjct: 52 TPLHVSAGNGNVDIVKYLLAWTGSDKVELEAMNTYGETPLHMAAKNGCN---EAAKLLLE 108
Query: 113 AGADVD 118
+GA ++
Sbjct: 109 SGAFIE 114
>At2g01680 unknown protein
Length = 532
Score = 32.3 bits (72), Expect = 0.16
Identities = 25/69 (36%), Positives = 37/69 (53%), Gaps = 6/69 (8%)
Query: 54 RTPLMIASLFGSIRVVKYIIETNMVNVNMAIGSENVTALHCAVAGGSKSKFEIVKLLLDA 113
+T L A +G +R+VK +IE + V + + TALH AV G S E+V+ +L
Sbjct: 163 KTSLHTAGRYGLLRIVKALIEKDAAIVGVK-DKKGQTALHMAVKGRS---LEVVEEILQ- 217
Query: 114 GADVDFLDE 122
AD L+E
Sbjct: 218 -ADYTILNE 225
>At1g14000 putative protein kinase
Length = 438
Score = 32.3 bits (72), Expect = 0.16
Identities = 15/24 (62%), Positives = 19/24 (78%)
Query: 51 YEKRTPLMIASLFGSIRVVKYIIE 74
Y+KRTPL +ASL G I VVK ++E
Sbjct: 69 YDKRTPLHVASLHGWIDVVKCLLE 92
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.135 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,178,501
Number of Sequences: 26719
Number of extensions: 163799
Number of successful extensions: 480
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 449
Number of HSP's gapped (non-prelim): 48
length of query: 193
length of database: 11,318,596
effective HSP length: 94
effective length of query: 99
effective length of database: 8,807,010
effective search space: 871893990
effective search space used: 871893990
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC142498.2