BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140913.3 - phase: 0
(476 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g16690 Unknown protein 32 0.59
At5g52280 hyaluronan mediated motility receptor-like protein 32 0.77
At3g51730 unknown protein 32 1.0
At1g79830 unknown protein (At1g79830) 32 1.0
At5g53020 putative protein 31 1.7
At5g19900 PRLI-interacting factor A 31 1.7
At4g08350 putative protein 31 1.7
At5g49680 putative protein 30 2.2
At5g49030 isoleucyl-tRNA synthetase 30 2.2
At5g03060 putative protein 30 3.8
At4g36990 heat shock transcription factor HSF4 30 3.8
At5g61490 putative protein 29 5.0
At4g31340 unknown protein 29 5.0
At2g18540 putative vicilin storage protein (globulin-like) 29 5.0
At4g12780 auxilin-like protein 29 6.5
At2g25010 unknown protein 29 6.5
At1g73860 kinesin-related protein 29 6.5
At1g37160 hypothetical protein 29 6.5
At1g25560 unknown protein 29 6.5
At4g26780 grpE like protein 28 8.5
>At1g16690 Unknown protein
Length = 439
Score = 32.3 bits (72), Expect = 0.59
Identities = 44/165 (26%), Positives = 77/165 (46%), Gaps = 25/165 (15%)
Query: 327 KSVRHLGVRSGVAHEAYTQWVIDRAEEIGMPYPAMRYVSSSTP---SMPLPLLPATQDMY 383
+++++L ++ GV H Y+ W R EI P +R + P + P + + +
Sbjct: 173 EALKYLSIKYGVFHAIYSYWKNKR--EIWQK-PILRRLQPPPPVNDTNPYNVFRPREKAH 229
Query: 384 QEHL-AMESRE---------KQVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKAR 433
+ H M+ RE +QV + +QA+ ++ L R+E+K + E++ R
Sbjct: 230 RLHARRMQQRENNAQSFEKLRQV-RRNLDQAKTILEALIKREEKKRD---FMASEVSLQR 285
Query: 434 RELEEKDEL-LMRDSKRARGRRDFFA-RYCDSDSE---SDDPPTT 473
+L+ K+E L+ DS G A R+ S+ E SDDP TT
Sbjct: 286 IQLKYKNETELLEDSLALAGFPLSTAYRFGSSEDEFMDSDDPTTT 330
>At5g52280 hyaluronan mediated motility receptor-like protein
Length = 853
Score = 32.0 bits (71), Expect = 0.77
Identities = 33/167 (19%), Positives = 67/167 (39%), Gaps = 8/167 (4%)
Query: 307 TNAPTGQRKAFMDAWSKVRRKSVRHLGVRSGVAHEAYTQWVIDRAEEIGMPYPAMRYVSS 366
++A + + + W K R + R L + VA A + + ++ +R + +
Sbjct: 671 SSAAATETEKIIQEWRKERDEFERKLSLAKEVAKTAQKELTLTKSSNDDKE-TRLRNLKT 729
Query: 367 STPSMPLPLLPATQDMYQEHLAMESREKQVWKARYN---QAENLIMTLDGRDEQKTHENL 423
+ L QE + + KQV + + + E + LD R E ++ EN
Sbjct: 730 EVEGLSLQYSELQNSFVQEKMENDELRKQVSNLKVDIRRKEEEMTKILDARMEARSQENG 789
Query: 424 MLKKELAKARREL---EEKDELLMRDSKRARGR-RDFFARYCDSDSE 466
++ L+K EL + K+ + R+ K R + R+ + + E
Sbjct: 790 HKEENLSKLSDELAYCKNKNSSMERELKEMEERYSEISLRFAEVEGE 836
>At3g51730 unknown protein
Length = 213
Score = 31.6 bits (70), Expect = 1.0
Identities = 24/76 (31%), Positives = 33/76 (42%), Gaps = 17/76 (22%)
Query: 153 LLIYGLLLFPNLDNFVDMNAIEIFHSKNPVPTLL-----------------ADTYHAIHD 195
LL+ GL+L + +FVD E +K V TL A+ +HD
Sbjct: 10 LLLLGLILVSDARSFVDSTISEKVSNKEDVCTLCEEYVTDALSYLEKNVTQAEIIEDLHD 69
Query: 196 RTLKGRGYILCCISLL 211
R + RGY CISL+
Sbjct: 70 RCSQLRGYSQQCISLV 85
>At1g79830 unknown protein (At1g79830)
Length = 918
Score = 31.6 bits (70), Expect = 1.0
Identities = 25/90 (27%), Positives = 42/90 (45%), Gaps = 2/90 (2%)
Query: 367 STPSMPLPL--LPATQDMYQEHLAMESREKQVWKARYNQAENLIMTLDGRDEQKTHENLM 424
S PS P+ L L A Q + L E + + Y A+ TL+GR Q E
Sbjct: 670 SLPSSPIQLSCLRAEQGQLSKSLEKERQRAAENRQEYLAAKEEADTLEGRANQLEVEIRE 729
Query: 425 LKKELAKARRELEEKDELLMRDSKRARGRR 454
L+++ + +E+ +EL+ +D +R + R
Sbjct: 730 LRRKHKQELQEVLLHNELIQKDLEREKASR 759
>At5g53020 putative protein
Length = 720
Score = 30.8 bits (68), Expect = 1.7
Identities = 21/71 (29%), Positives = 37/71 (51%), Gaps = 3/71 (4%)
Query: 384 QEHLAMESREKQVW---KARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARRELEEKD 440
QEH + R+K+ + + Q E + + + E K HE L E+ K R++LE+KD
Sbjct: 155 QEHSSELWRQKKTFLELASSQRQLEAELSRANKQIEAKGHELEDLSLEINKMRKDLEQKD 214
Query: 441 ELLMRDSKRAR 451
+L K+++
Sbjct: 215 RILAVMMKKSK 225
>At5g19900 PRLI-interacting factor A
Length = 494
Score = 30.8 bits (68), Expect = 1.7
Identities = 22/71 (30%), Positives = 30/71 (41%)
Query: 402 NQAENLIMTLDGRDEQKTHENLMLKKELAKARRELEEKDELLMRDSKRARGRRDFFARYC 461
N EN + D Q ENL LK+ L REL + L +R +D
Sbjct: 306 NVLENRVDDQDSHIAQLEEENLTLKERLFLMERELGDMRRRLQYLERRDMVAQDANEEVV 365
Query: 462 DSDSESDDPPT 472
+++SESD T
Sbjct: 366 ENESESDGDDT 376
>At4g08350 putative protein
Length = 1054
Score = 30.8 bits (68), Expect = 1.7
Identities = 20/65 (30%), Positives = 30/65 (45%), Gaps = 3/65 (4%)
Query: 414 RDEQKTHENLMLKKELAKARRELEEKDELLMRD---SKRARGRRDFFARYCDSDSESDDP 470
RDE + +L + E EE++E R S+R RGR +F Y + DS+ +D
Sbjct: 5 RDEDDELDGDYEALDLEEEEEEDEEEEEERGRGGGGSRRKRGRSNFIDDYAEEDSQEEDD 64
Query: 471 PTTSY 475
Y
Sbjct: 65 DDEDY 69
>At5g49680 putative protein
Length = 1378
Score = 30.4 bits (67), Expect = 2.2
Identities = 29/117 (24%), Positives = 52/117 (43%), Gaps = 16/117 (13%)
Query: 368 TPSMPL-PLLPATQDMYQEHLAMESREKQVWK--------ARYNQAENLIMTLDGRDEQK 418
TP + + PL T + +M SR+ QV AR +A N + L G ++ +
Sbjct: 725 TPDLKVKPLKELTFNSRNITASMTSRQFQVMTDVLSNLLFARLPKAHNDSLKLSGEEDDE 784
Query: 419 THENL-------MLKKELAKARRELEEKDELLMRDSKRARGRRDFFARYCDSDSESD 468
E + + + ELAK E +E+D +++ D R + + + + + ESD
Sbjct: 785 VEEEIDEVVPDGIEEVELAKIELEAKERDRMMLLDDIRKLTQNESNSGNINLEKESD 841
>At5g49030 isoleucyl-tRNA synthetase
Length = 1093
Score = 30.4 bits (67), Expect = 2.2
Identities = 22/61 (36%), Positives = 28/61 (45%), Gaps = 3/61 (4%)
Query: 312 GQRKAFMDAWSKVRRKSVRHLGV-RSGVAHEAYTQWVIDRAEEIGMPYPAMRYVSSSTPS 370
G R A MDA + V K V H V R + + W I R G+P PA +V + P
Sbjct: 548 GFRTATMDAINNV--KWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPL 605
Query: 371 M 371
M
Sbjct: 606 M 606
>At5g03060 putative protein
Length = 292
Score = 29.6 bits (65), Expect = 3.8
Identities = 15/65 (23%), Positives = 33/65 (50%)
Query: 395 QVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARRELEEKDELLMRDSKRARGRR 454
++ K Y E +++ +Q N ++K + K +ELEEK L++D ++ R +
Sbjct: 40 EILKENYENLEKDYKSIEESFKQMNEMNEIMKFQYQKQIKELEEKILSLLKDLEKERSEK 99
Query: 455 DFFAR 459
+ + +
Sbjct: 100 EEYMK 104
>At4g36990 heat shock transcription factor HSF4
Length = 284
Score = 29.6 bits (65), Expect = 3.8
Identities = 13/34 (38%), Positives = 21/34 (61%)
Query: 405 ENLIMTLDGRDEQKTHENLMLKKELAKARRELEE 438
EN++ L G +E+ EN L ELA A+++ +E
Sbjct: 150 ENMVADLSGENEKLKRENNNLSSELAAAKKQRDE 183
>At5g61490 putative protein
Length = 260
Score = 29.3 bits (64), Expect = 5.0
Identities = 14/42 (33%), Positives = 23/42 (54%)
Query: 205 LCCISLLYRWFISHLPSSFHDNSENWSYSQRIMALTPNEVVW 246
LC SLL + + L +FH + S + +M+LT ++ VW
Sbjct: 118 LCNTSLLQPFMLDRLYDAFHVFQTDPSVQRMVMSLTSDKAVW 159
>At4g31340 unknown protein
Length = 437
Score = 29.3 bits (64), Expect = 5.0
Identities = 16/70 (22%), Positives = 35/70 (49%)
Query: 381 DMYQEHLAMESREKQVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARRELEEKD 440
++ + L +++EK +AR N+AE + L+ ++ N K ++ K R ++ +
Sbjct: 122 EVLKNFLEQKNKEKDSTEARTNEAEKKLRELNSSLDKLQKTNEEQKNKIGKLERAIKIAE 181
Query: 441 ELLMRDSKRA 450
E ++R A
Sbjct: 182 EEMLRTKLEA 191
>At2g18540 putative vicilin storage protein (globulin-like)
Length = 699
Score = 29.3 bits (64), Expect = 5.0
Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 6/68 (8%)
Query: 384 QEHLAMESREKQVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARRELEEKDELL 443
+E + + RE+Q K R +A +E+K E + ++E + R+E EE + +
Sbjct: 534 REEVERKRREEQERKRREEEARKR------EEERKREEEMAKRREQERQRKEREEVERKI 587
Query: 444 MRDSKRAR 451
+ +R R
Sbjct: 588 REEQERKR 595
>At4g12780 auxilin-like protein
Length = 904
Score = 28.9 bits (63), Expect = 6.5
Identities = 17/66 (25%), Positives = 32/66 (47%)
Query: 388 AMESREKQVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARRELEEKDELLMRDS 447
A E REK+ KA ++ + D R+ + + + L +E A+ E+E+ E +
Sbjct: 474 AKERREKENLKASRSREGDHTENYDSRERELREKQVRLDRERAEREAEMEKAQEREKEER 533
Query: 448 KRARGR 453
+R + R
Sbjct: 534 EREQKR 539
>At2g25010 unknown protein
Length = 509
Score = 28.9 bits (63), Expect = 6.5
Identities = 35/139 (25%), Positives = 54/139 (38%), Gaps = 33/139 (23%)
Query: 186 LADTYHAIHDRTLKGRGYILCCISLLYRWFISHLPSSFHDNSEN-------W-------- 230
LA Y A+ + +LK + I C++LL W HL + SE W
Sbjct: 221 LACLYRALGNASLKSQSNICGCLTLLQCWSYFHLDIGRPEKSEACFPLALLWKGKGSRSK 280
Query: 231 ----SYSQRIMALTPNEVVW--------LTPAAQVKEIIMGCGDFLNVPLLGIRGGINYN 278
Y + + L P+++ W L P ++I+G V I +
Sbjct: 281 TDLSEYRRELDDLDPSKITWCPYERFENLIPPHIKAKLILGRSKTTLVCFEKIE---LHF 337
Query: 279 PELAMRQFGFPMKSKPINL 297
P+ +RQFG K +PI L
Sbjct: 338 PDRCLRQFG---KRQPIPL 353
>At1g73860 kinesin-related protein
Length = 1050
Score = 28.9 bits (63), Expect = 6.5
Identities = 14/59 (23%), Positives = 32/59 (53%)
Query: 393 EKQVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARRELEEKDELLMRDSKRAR 451
EK +W+ + E ++ L +++Q E LK+EL +R+ E++ + +K+++
Sbjct: 238 EKSMWEEKKKHEEEDMVKLMKQNDQHNLEISALKQELETTKRKYEQQYSQIESQTKKSK 296
>At1g37160 hypothetical protein
Length = 480
Score = 28.9 bits (63), Expect = 6.5
Identities = 14/46 (30%), Positives = 25/46 (53%)
Query: 401 YNQAENLIMTLDGRDEQKTHENLMLKKELAKARRELEEKDELLMRD 446
YN E + EQ+ +E+ K E+A+ +R + E+ EL+ +D
Sbjct: 14 YNMDETESSSSRPEREQREYESFRRKAEIARGKRAMTERYELIDKD 59
>At1g25560 unknown protein
Length = 361
Score = 28.9 bits (63), Expect = 6.5
Identities = 28/124 (22%), Positives = 48/124 (38%), Gaps = 33/124 (26%)
Query: 313 QRKAFMDAWSKVRRKSVRHLGVRSGVAHEAYTQWVIDRAEEIGMPYPAMRYVSSSTPS-- 370
++ + D + + RRK V G RSG+ Y + RA E+ + + TPS
Sbjct: 152 RKHTYADEFEQSRRKFVNGDGKRSGLETATYGNDAVLRAREV-------LFEKTVTPSDV 204
Query: 371 ----------------MPLPLLPATQDMYQEH-----LAMESREKQVWKARY---NQAEN 406
PLP + M + +E R +VW+ RY N +++
Sbjct: 205 GKLNRLVIPKQHAEKHFPLPAMTTAMGMNPSPTKGVLINLEDRTGKVWRFRYSYWNSSQS 264
Query: 407 LIMT 410
++T
Sbjct: 265 YVLT 268
>At4g26780 grpE like protein
Length = 327
Score = 28.5 bits (62), Expect = 8.5
Identities = 19/61 (31%), Positives = 31/61 (50%), Gaps = 6/61 (9%)
Query: 415 DEQKTHENLML---KKELAKARR---ELEEKDELLMRDSKRARGRRDFFARYCDSDSESD 468
DE+KTH +K A+A + + E KD + ++ +G + + +SDSESD
Sbjct: 76 DEKKTHTEASKTSEEKPTAEANQPGLDSESKDSVTDSAKRKRKGAKGAASSSSESDSESD 135
Query: 469 D 469
D
Sbjct: 136 D 136
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.136 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,123,662
Number of Sequences: 26719
Number of extensions: 498094
Number of successful extensions: 1386
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 1370
Number of HSP's gapped (non-prelim): 29
length of query: 476
length of database: 11,318,596
effective HSP length: 103
effective length of query: 373
effective length of database: 8,566,539
effective search space: 3195319047
effective search space used: 3195319047
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC140913.3