BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140850.3 + phase: 0 /pseudo
(650 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g14400 putative retroelement pol polyprotein 121 1e-27
At4g03800 117 2e-26
At2g15100 putative retroelement pol polyprotein 116 3e-26
At1g37200 hypothetical protein 114 1e-25
At1g20390 hypothetical protein 113 4e-25
At2g15410 putative retroelement pol polyprotein 112 5e-25
At2g14380 putative retroelement pol polyprotein 111 1e-24
At2g13210 pseudogene 110 3e-24
At2g13330 F14O4.9 91 2e-18
At1g34080 putative protein 77 3e-14
At2g10780 pseudogene 75 9e-14
At2g07660 putative retroelement pol polyprotein 75 9e-14
At3g11970 hypothetical protein 75 1e-13
At1g36590 hypothetical protein 75 1e-13
At2g14040 putative retroelement pol polyprotein 74 3e-13
At2g05610 putative retroelement pol polyprotein 73 4e-13
At4g10580 putative reverse-transcriptase -like protein 73 6e-13
At1g35370 hypothetical protein 70 5e-12
At2g04670 putative retroelement pol polyprotein 68 1e-11
At4g07600 68 2e-11
>At2g14400 putative retroelement pol polyprotein
Length = 1466
Score = 121 bits (303), Expect = 1e-27
Identities = 55/82 (67%), Positives = 69/82 (84%)
Query: 99 VEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMD 158
V+YP+W+AN V V KK+GK R+C+DF DLNKA PKD+FPLPHID LV++TA +++ SFMD
Sbjct: 496 VQYPDWLANTVVVKKKNGKDRVCIDFTDLNKACPKDSFPLPHIDRLVESTAGNELLSFMD 555
Query: 159 GFSGYNLIKMSPEDREKTSFIT 180
FSGYN I M+PED+EKT FIT
Sbjct: 556 AFSGYNQIMMNPEDQEKTLFIT 577
>At4g03800
Length = 637
Score = 117 bits (294), Expect = 2e-26
Identities = 54/82 (65%), Positives = 68/82 (82%)
Query: 99 VEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMD 158
V+YP+WVA V V KK+GK R+C+DF DLNKA PKD+FPLPHID LV++TA +++ +FMD
Sbjct: 373 VQYPDWVAITVVVKKKNGKDRVCIDFTDLNKACPKDSFPLPHIDRLVESTAGNELLTFMD 432
Query: 159 GFSGYNLIKMSPEDREKTSFIT 180
F GYN I M+PED+EKTSFIT
Sbjct: 433 AFLGYNQIMMNPEDQEKTSFIT 454
>At2g15100 putative retroelement pol polyprotein
Length = 1329
Score = 116 bits (291), Expect = 3e-26
Identities = 56/82 (68%), Positives = 66/82 (80%)
Query: 99 VEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMD 158
V+YPEW+AN V V KK+GK R+CVDF DLNKA KD FPLPHID LV++T ++ SFMD
Sbjct: 464 VKYPEWLANPVVVKKKNGKWRVCVDFTDLNKACSKDFFPLPHIDRLVESTTGHEMLSFMD 523
Query: 159 GFSGYNLIKMSPEDREKTSFIT 180
FSGYN I M+PED+EKTSFIT
Sbjct: 524 AFSGYNQILMNPEDQEKTSFIT 545
>At1g37200 hypothetical protein
Length = 1564
Score = 114 bits (286), Expect = 1e-25
Identities = 55/92 (59%), Positives = 71/92 (76%), Gaps = 1/92 (1%)
Query: 90 RLMRV-FLMTVEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNT 148
RL+++ + V+YP+W+AN V V K+GK R+C+DF DLNKA PKD+FPLPHID LV T
Sbjct: 707 RLLKIGSIREVKYPDWLANPVVVKNKNGKWRVCIDFMDLNKACPKDSFPLPHIDRLVKAT 766
Query: 149 AQSKVFSFMDGFSGYNLIKMSPEDREKTSFIT 180
A ++ SFMD FSGYN I M P+D+EKT+FIT
Sbjct: 767 AGHELLSFMDAFSGYNQILMRPDDQEKTAFIT 798
>At1g20390 hypothetical protein
Length = 1791
Score = 113 bits (282), Expect = 4e-25
Identities = 52/85 (61%), Positives = 68/85 (79%)
Query: 96 LMTVEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFS 155
++ V+YPEW+AN V V KK+GK R+CVD+ DLNKA PKD++PLPHID LV+ T+ + + S
Sbjct: 806 IIEVKYPEWLANPVVVKKKNGKWRVCVDYTDLNKACPKDSYPLPHIDRLVEATSGNGLLS 865
Query: 156 FMDGFSGYNLIKMSPEDREKTSFIT 180
FMD FSGYN I M +D+EKTSF+T
Sbjct: 866 FMDAFSGYNQILMHKDDQEKTSFVT 890
>At2g15410 putative retroelement pol polyprotein
Length = 1787
Score = 112 bits (281), Expect = 5e-25
Identities = 53/85 (62%), Positives = 65/85 (76%)
Query: 96 LMTVEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFS 155
++ V YP+W+ N V V KK+GK R+C+DF DLNKA PKD+FPLPHID LV+ TA +++ S
Sbjct: 811 IVEVRYPDWLRNPVVVKKKNGKWRVCIDFTDLNKACPKDSFPLPHIDRLVEATAGNELLS 870
Query: 156 FMDGFSGYNLIKMSPEDREKTSFIT 180
FMD FSGYN I M DREKT FIT
Sbjct: 871 FMDAFSGYNQILMHQNDREKTVFIT 895
>At2g14380 putative retroelement pol polyprotein
Length = 764
Score = 111 bits (277), Expect = 1e-24
Identities = 51/84 (60%), Positives = 66/84 (77%)
Query: 96 LMTVEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFS 155
++ V+YPEW+AN V V KK+ K R+C+DF DLNKA PKD+FPLPHID +V+ T +++ S
Sbjct: 526 IVEVKYPEWLANPVVVKKKNDKWRVCIDFTDLNKACPKDSFPLPHIDRMVEATTGNELLS 585
Query: 156 FMDGFSGYNLIKMSPEDREKTSFI 179
FMD FSGYN I M +D+EKTSFI
Sbjct: 586 FMDAFSGYNQIPMHKDDQEKTSFI 609
>At2g13210 pseudogene
Length = 152
Score = 110 bits (274), Expect = 3e-24
Identities = 52/92 (56%), Positives = 71/92 (76%), Gaps = 1/92 (1%)
Query: 90 RLMRVFLMT-VEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNT 148
+L+R+ +T +YPEW+AN V V KK+GK R+C+DF DLNKA PKD+FP+ HI LV++T
Sbjct: 55 KLLRIGSITKAKYPEWLANPVVVKKKNGKWRVCIDFTDLNKACPKDSFPMSHIGRLVEST 114
Query: 149 AQSKVFSFMDGFSGYNLIKMSPEDREKTSFIT 180
+ K+ SFMD F+GYN I M+ ED+EKT+F T
Sbjct: 115 SGDKLLSFMDAFAGYNQIMMNSEDQEKTTFYT 146
>At2g13330 F14O4.9
Length = 889
Score = 90.9 bits (224), Expect = 2e-18
Identities = 42/68 (61%), Positives = 53/68 (77%)
Query: 113 KKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPED 172
+K+GK R+C+DFRDLNKA PKD+FPLPHID LV+ T + + SFMD F GYN I M +
Sbjct: 289 EKNGKWRVCIDFRDLNKACPKDSFPLPHIDRLVEATVEHEKLSFMDAFYGYNQILMRRDG 348
Query: 173 REKTSFIT 180
+EKT+FIT
Sbjct: 349 QEKTAFIT 356
>At1g34080 putative protein
Length = 811
Score = 77.0 bits (188), Expect = 3e-14
Identities = 38/59 (64%), Positives = 47/59 (79%), Gaps = 1/59 (1%)
Query: 90 RLMRVFLMT-VEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDN 147
RL++ L+ V+YPEW+AN V V KK+GK R+CVDF DLNKA PKD FPLPHID LV++
Sbjct: 670 RLLKAGLIREVKYPEWLANPVVVKKKNGKWRVCVDFTDLNKACPKDFFPLPHIDRLVES 728
>At2g10780 pseudogene
Length = 1611
Score = 75.5 bits (184), Expect = 9e-14
Identities = 39/101 (38%), Positives = 62/101 (60%), Gaps = 1/101 (0%)
Query: 80 LSRLRVRFKSRLMRVFLMTVEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLP 139
+++L+ + + L + F+ P W A ++ V KKDG R+C+D+R LNK + K+ +PLP
Sbjct: 672 MAKLKKQLEELLDKGFIRPSSSP-WGAPVLFVKKKDGSFRLCIDYRGLNKVTVKNKYPLP 730
Query: 140 HIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREKTSFIT 180
ID L+D ++ FS +D SGY+ I + P D KT+F T
Sbjct: 731 RIDELMDQLGGAQWFSKIDLASGYHQIPIEPTDVRKTAFRT 771
>At2g07660 putative retroelement pol polyprotein
Length = 949
Score = 75.5 bits (184), Expect = 9e-14
Identities = 39/101 (38%), Positives = 61/101 (59%), Gaps = 1/101 (0%)
Query: 80 LSRLRVRFKSRLMRVFLMTVEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLP 139
++ L+ + + L + F+ P W A ++ V KKDG R+C+D+R LNK + K+ +PLP
Sbjct: 167 MAELKKQLEELLAKGFIRPSSSP-WGAPVLFVKKKDGSFRLCIDYRGLNKVTVKNKYPLP 225
Query: 140 HIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREKTSFIT 180
ID L+D ++ FS +D SGY+ I + P D KT+F T
Sbjct: 226 RIDELMDQLGGAQWFSKIDLASGYHQIPIEPTDVRKTAFRT 266
>At3g11970 hypothetical protein
Length = 1499
Score = 75.1 bits (183), Expect = 1e-13
Identities = 34/78 (43%), Positives = 55/78 (69%)
Query: 104 WVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGY 163
+ + +V V KKDG R+CVD+R+LN + KD+FP+P I+ L+D + +FS +D +GY
Sbjct: 637 YASPVVLVKKKDGTWRLCVDYRELNGMTVKDSFPIPLIEDLMDELGGAVIFSKIDLRAGY 696
Query: 164 NLIKMSPEDREKTSFITH 181
+ ++M P+D +KT+F TH
Sbjct: 697 HQVRMDPDDIQKTAFKTH 714
>At1g36590 hypothetical protein
Length = 1499
Score = 75.1 bits (183), Expect = 1e-13
Identities = 34/78 (43%), Positives = 55/78 (69%)
Query: 104 WVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGY 163
+ + +V V KKDG R+CVD+R+LN + KD+FP+P I+ L+D + +FS +D +GY
Sbjct: 637 YASPVVLVKKKDGTWRLCVDYRELNGMTVKDSFPIPLIEDLMDELGGAVIFSKIDLRAGY 696
Query: 164 NLIKMSPEDREKTSFITH 181
+ ++M P+D +KT+F TH
Sbjct: 697 HQVRMDPDDIQKTAFKTH 714
>At2g14040 putative retroelement pol polyprotein
Length = 841
Score = 73.9 bits (180), Expect = 3e-13
Identities = 41/120 (34%), Positives = 63/120 (52%), Gaps = 22/120 (18%)
Query: 81 SRLRVRFKSRLMRVFLMTVEYP----EWVANIVPVPKKDGKV------------------ 118
S LR K +M++ + YP WV+ + VPKK G +
Sbjct: 384 SNLRDVVKKEIMKLLDAGIIYPISDSTWVSPVHVVPKKGGVIVIKNEKNELIPTRTVTGH 443
Query: 119 RMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREKTSF 178
RMC+D+R LN A+ KDNFPL ID +++ + + F+DG+ G+ I + P+D+EKT+F
Sbjct: 444 RMCIDYRKLNSATRKDNFPLSFIDQMLERLSNQPYYCFLDGYLGFFQILIHPDDQEKTTF 503
>At2g05610 putative retroelement pol polyprotein
Length = 780
Score = 73.2 bits (178), Expect = 4e-13
Identities = 35/78 (44%), Positives = 52/78 (65%)
Query: 104 WVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGY 163
+ + +V V KKDG R+CVD+R+LN + KD FP+P I+ L+D S V+S +D +GY
Sbjct: 79 YASPVVLVKKKDGTWRLCVDYRELNGMTVKDRFPIPLIEDLMDELGGSNVYSKIDLRAGY 138
Query: 164 NLIKMSPEDREKTSFITH 181
+ ++M P D KT+F TH
Sbjct: 139 HQVRMDPLDIHKTAFKTH 156
>At4g10580 putative reverse-transcriptase -like protein
Length = 1240
Score = 72.8 bits (177), Expect = 6e-13
Identities = 38/101 (37%), Positives = 61/101 (59%), Gaps = 1/101 (0%)
Query: 80 LSRLRVRFKSRLMRVFLMTVEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLP 139
++ L+ + K L + F+ P W A ++ V KKDG R+C+D+R+LN+ + K+ +PLP
Sbjct: 504 MAELKKQLKDLLGKGFIRPSTSP-WGAPVLFVKKKDGSFRLCIDYRELNRVTVKNRYPLP 562
Query: 140 HIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREKTSFIT 180
ID L+D + FS +D SGY+ I ++ D KT+F T
Sbjct: 563 RIDELLDQLRGATCFSKIDLTSGYHQIPIAEADVRKTAFRT 603
>At1g35370 hypothetical protein
Length = 1447
Score = 69.7 bits (169), Expect = 5e-12
Identities = 34/74 (45%), Positives = 50/74 (66%)
Query: 108 IVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIK 167
+V V KKDG R+CVD+ +LN + KD F +P I+ L+D S VFS +D +GY+ ++
Sbjct: 620 VVLVKKKDGTWRLCVDYTELNGMTVKDRFLIPLIEDLMDELGGSVVFSKIDLRAGYHQVR 679
Query: 168 MSPEDREKTSFITH 181
M P+D +KT+F TH
Sbjct: 680 MDPDDIQKTAFKTH 693
>At2g04670 putative retroelement pol polyprotein
Length = 1411
Score = 68.2 bits (165), Expect = 1e-11
Identities = 37/101 (36%), Positives = 59/101 (57%), Gaps = 1/101 (0%)
Query: 80 LSRLRVRFKSRLMRVFLMTVEYPEWVANIVPVPKKDGKVRMCVDFRDLNKASPKDNFPLP 139
++ L+ + + L + F+ P W A ++ V KKDG R+C+D+R LN + K+ +PLP
Sbjct: 530 MTELKKQLEDLLGKGFIRPSTSP-WGAPVLFVKKKDGSFRLCIDYRGLNWVTVKNKYPLP 588
Query: 140 HIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREKTSFIT 180
ID L+D + FS +D SGY+ I ++ D KT+F T
Sbjct: 589 RIDELLDQLRGATCFSKIDLTSGYHQIPIAEADVRKTAFRT 629
>At4g07600
Length = 630
Score = 67.8 bits (164), Expect = 2e-11
Identities = 30/71 (42%), Positives = 47/71 (65%), Gaps = 1/71 (1%)
Query: 108 IVPVPKKDGKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIK 167
++P+ G +RMC+D+R+LN AS D+FPLP D +++ A + F+DG+SG+ I
Sbjct: 346 LIPIRTITG-LRMCIDYRNLNAASRNDHFPLPFTDQMLERLANHPYYCFLDGYSGFFQIP 404
Query: 168 MSPEDREKTSF 178
+ P D EKT+F
Sbjct: 405 IHPNDHEKTTF 415
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.347 0.151 0.514
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,634,722
Number of Sequences: 26719
Number of extensions: 475035
Number of successful extensions: 1504
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1468
Number of HSP's gapped (non-prelim): 36
length of query: 650
length of database: 11,318,596
effective HSP length: 106
effective length of query: 544
effective length of database: 8,486,382
effective search space: 4616591808
effective search space used: 4616591808
T: 11
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.7 bits)
S2: 63 (28.9 bits)
Medicago: description of AC140850.3