BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140773.5 - phase: 0 /pseudo
(194 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g18510 putative protein 33 0.12
At5g48600 chromosome condensation protein 32 0.21
At5g44410 berberine bridge enzyme-like protein 31 0.47
At4g20820 reticuline oxidase - like protein 31 0.47
At5g64220 Calmodulin-binding transcription activator 2 (CAMTA2) 30 0.61
At5g65710 receptor protein kinase-like protein 29 1.4
At1g50830 hypothetical protein 29 1.4
At1g50820 hypothetical protein 29 1.4
At5g44440 berberine bridge enzyme-like protein 29 1.8
At4g11070 WRKY transcription factor 41 (WRKY41) 28 2.3
At1g50760 hypothetical protein 28 2.3
At1g50770 hypothetical protein 28 3.0
At5g48410 ligand-gated ion channel protein-like; glutamate recep... 28 4.0
At1g50750 hypothetical protein 28 4.0
At1g07710 hypothetical protein 28 4.0
At2g43290 putative calcium binding protein 27 5.2
At2g18900 unknown protein 27 5.2
At1g50790 hypothetical protein 27 5.2
At1g14440 unknown protein 27 5.2
At5g55720 pectate lyase 27 6.8
>At5g18510 putative protein
Length = 702
Score = 32.7 bits (73), Expect = 0.12
Identities = 11/28 (39%), Positives = 19/28 (67%)
Query: 167 YLDWLDKVEKIKGPFWKEMGVFDLIQLS 194
+L WL K++ + P WK+ G+F+ I+ S
Sbjct: 60 FLSWLGKMQALYEPIWKKAGIFEAIKAS 87
>At5g48600 chromosome condensation protein
Length = 1241
Score = 32.0 bits (71), Expect = 0.21
Identities = 32/104 (30%), Positives = 44/104 (41%), Gaps = 15/104 (14%)
Query: 86 EPDVKEAEDKIWKSQVIISCPNSSHEYL-----------GPLSDESDDQKKLDAVFPWNE 134
E +V E ++ KSQ I NS H YL P +DE D K+L + E
Sbjct: 753 ENEVSGLEMELAKSQREIESLNSEHNYLEKQLASLEAASQPKTDEIDRLKELKKIISKEE 812
Query: 135 AQLPLIFSKGPYDLSFLKSKFRTEPKIENNEKYLDWLDKVEKIK 178
++ + KG LK K +T + EK KVEKI+
Sbjct: 813 KEIENL-EKGSKQ---LKDKLQTNIENAGGEKLKGQKAKVEKIQ 852
>At5g44410 berberine bridge enzyme-like protein
Length = 535
Score = 30.8 bits (68), Expect = 0.47
Identities = 26/102 (25%), Positives = 42/102 (40%), Gaps = 14/102 (13%)
Query: 83 FMEEPDVKEAEDKIWKSQVIISCPNS--SHEYLGPLSDESDDQKKLDAVFPWNEAQLPLI 140
F+EEP K A +W+ P + + L P + + + FP E L
Sbjct: 370 FIEEPIPKTAIQYLWRR---FEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNL--- 423
Query: 141 FSKGPYDLSFLKSKFRTEPKIENN-EKYLDWLDKVEKIKGPF 181
Y++ +L E K + N EKYL W++ V + P+
Sbjct: 424 -----YEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPY 460
>At4g20820 reticuline oxidase - like protein
Length = 532
Score = 30.8 bits (68), Expect = 0.47
Identities = 24/109 (22%), Positives = 49/109 (44%), Gaps = 18/109 (16%)
Query: 68 TKQHEPNVNLPVNTQFMEEPDVKEAEDKIWKSQVIISCPNSSHEYL--GPLSDESDDQKK 125
TK+ ++ + F++EP K A K+W+ + P + H L P + +
Sbjct: 359 TKRTRASLAFKAKSDFVQEPMPKTAISKLWRR---LQEPEAEHAQLIFTPFGGKMSEIAD 415
Query: 126 LDAVFPWNEAQLPLIFSKGPYDLSFLKSKFRTEPKIENNEKYLDWLDKV 174
+ FP + + Y++ +L + +R + K EKY+ W+++V
Sbjct: 416 YETPFPHRKGNI--------YEIQYL-NYWRGDVK----EKYMRWVERV 451
>At5g64220 Calmodulin-binding transcription activator 2 (CAMTA2)
Length = 1008
Score = 30.4 bits (67), Expect = 0.61
Identities = 36/169 (21%), Positives = 69/169 (40%), Gaps = 12/169 (7%)
Query: 37 SSTLRFQNIVHNS----TKNFEIFMARVEGSSSQPTKQHEPNVNLPVNTQFMEEPDVK-- 90
+S R+QN+ +N+ T+ + +EG + + + ++ P+ +Q + V
Sbjct: 214 NSLARYQNLPYNAPLTQTQPSTFGLIPMEGKTEKGSLLTSEHLRNPLQSQTPVQESVPLQ 273
Query: 91 ----EAEDKIWKSQVIISCPNSSHEYLGPLSD--ESDDQKKLDAVFPWNEAQLPLIFSKG 144
++ + + + +HE G S S DQ+ P+ + I G
Sbjct: 274 KWPMDSHSGMTDATDLALFGQGAHENFGTFSSLLGSQDQQSSSFQAPFTNNEAAYIPKLG 333
Query: 145 PYDLSFLKSKFRTEPKIENNEKYLDWLDKVEKIKGPFWKEMGVFDLIQL 193
P DL + S +T P + K D L KV+ KE+G + +Q+
Sbjct: 334 PEDLIYEASANQTLPLRKALLKKEDSLKKVDSFSRWVSKELGEMEDLQM 382
>At5g65710 receptor protein kinase-like protein
Length = 976
Score = 29.3 bits (64), Expect = 1.4
Identities = 19/62 (30%), Positives = 28/62 (44%)
Query: 93 EDKIWKSQVIISCPNSSHEYLGPLSDESDDQKKLDAVFPWNEAQLPLIFSKGPYDLSFLK 152
ED S V +SC S+ Y+ P + + V+ + L LI K P D SF +
Sbjct: 828 EDNDGVSDVSMSCVAGSYGYIAPEYGYTSKVNEKSDVYSFGVVLLELITGKRPNDSSFGE 887
Query: 153 SK 154
+K
Sbjct: 888 NK 889
>At1g50830 hypothetical protein
Length = 768
Score = 29.3 bits (64), Expect = 1.4
Identities = 9/28 (32%), Positives = 19/28 (67%)
Query: 167 YLDWLDKVEKIKGPFWKEMGVFDLIQLS 194
++ WL K+E + W++ G+F+ I++S
Sbjct: 82 FVSWLAKMEALHAQTWRKAGIFEAIKVS 109
>At1g50820 hypothetical protein
Length = 528
Score = 29.3 bits (64), Expect = 1.4
Identities = 8/28 (28%), Positives = 17/28 (60%)
Query: 164 NEKYLDWLDKVEKIKGPFWKEMGVFDLI 191
N+K+ W K+ + P W++ G+F+ +
Sbjct: 57 NKKFKSWARKMASLHEPIWRKAGIFEAV 84
>At5g44440 berberine bridge enzyme-like protein
Length = 533
Score = 28.9 bits (63), Expect = 1.8
Identities = 23/117 (19%), Positives = 47/117 (39%), Gaps = 14/117 (11%)
Query: 68 TKQHEPNVNLPVNTQFMEEPDVKEAEDKIWKSQVIISCPNS--SHEYLGPLSDESDDQKK 125
T + +++ F++EP + A +IW+ + P + L P + + +
Sbjct: 356 TNRERTSLSFKGKDDFVQEPIPEAAIQEIWRR---LEAPEARLGKIILTPFGGKMSEMAE 412
Query: 126 LDAVFPWNEAQLPLIFSKGPYDLSFLKSKFRTEPKIENN-EKYLDWLDKVEKIKGPF 181
+ FP L Y++ ++ E K + +KYL W+D V + P+
Sbjct: 413 YETPFPHRGGNL--------YEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPY 461
>At4g11070 WRKY transcription factor 41 (WRKY41)
Length = 313
Score = 28.5 bits (62), Expect = 2.3
Identities = 34/132 (25%), Positives = 53/132 (39%), Gaps = 21/132 (15%)
Query: 33 SPSPSSTLRF------QNIVHNSTKNFEIFMARVEGSSSQPTKQHEPNVNLPVNTQFMEE 86
SPS S++ + +N++HN +F+ + + GS T QH P + L
Sbjct: 30 SPSLSASSSYLTTEIKENLLHNIVSSFKKAILMLNGS----TTQHNPTIEL--------A 77
Query: 87 PDVKEAEDKIWKSQVIISCPNSSHEYLGPLSDESDDQKKLDAVFP-WNEAQ--LPLIFSK 143
PD K+ S I+ S E+ S E + K + P W E P +
Sbjct: 78 PDPLAHPGKVPGSPASITGNPRSEEFFNVRSKEFNLSSKKRKMLPKWTEQVRISPERGLE 137
Query: 144 GPYDLSFLKSKF 155
GP+D F K+
Sbjct: 138 GPHDDIFSWRKY 149
>At1g50760 hypothetical protein
Length = 649
Score = 28.5 bits (62), Expect = 2.3
Identities = 9/31 (29%), Positives = 17/31 (54%)
Query: 164 NEKYLDWLDKVEKIKGPFWKEMGVFDLIQLS 194
N K+ W K+ + P W++ G+F+ + S
Sbjct: 50 NMKFKRWARKMSALHEPIWRKAGIFEAVNAS 80
>At1g50770 hypothetical protein
Length = 632
Score = 28.1 bits (61), Expect = 3.0
Identities = 12/50 (24%), Positives = 22/50 (44%)
Query: 145 PYDLSFLKSKFRTEPKIENNEKYLDWLDKVEKIKGPFWKEMGVFDLIQLS 194
P +L FL N+ + W K+ + P W++ G+F+ + S
Sbjct: 41 PPELKFLSFSVSFNGWRFPNKNFKSWARKMAALHEPIWRKAGIFEAVTAS 90
>At5g48410 ligand-gated ion channel protein-like; glutamate
receptor-like (AtGLR1.3)
Length = 860
Score = 27.7 bits (60), Expect = 4.0
Identities = 40/148 (27%), Positives = 65/148 (43%), Gaps = 26/148 (17%)
Query: 20 TLTSFLLSLLLLFSPSPSSTLRFQNIVHNSTKNFEIFMARVEGSSSQPTKQHEPNVNLPV 79
TL SFLL LLL S S +ST +N + K +I + V S K + +V++ +
Sbjct: 10 TLLSFLLVLLLFISRSFAST---KNDDVDGGKRVQIRVGLVLDLGSLKGKIVKNSVSMAL 66
Query: 80 NTQFMEEPDVKEAEDKIWKSQVIISCPNSSHEYLGPLSDESDDQKK--LDAVFPWN---- 133
+ + D +K++V +S NS E L L+ D K ++A+ N
Sbjct: 67 SYFYAIHND--------YKTRVSVSLRNSHGEPLLALASAVDLLKTEGVEAIIGGNSLLE 118
Query: 134 ---------EAQLPLIFSKGPYDLSFLK 152
+A++P+I P+ LS K
Sbjct: 119 AKLLGELGEKARVPMISLDSPFSLSLSK 146
>At1g50750 hypothetical protein
Length = 816
Score = 27.7 bits (60), Expect = 4.0
Identities = 9/31 (29%), Positives = 17/31 (54%)
Query: 164 NEKYLDWLDKVEKIKGPFWKEMGVFDLIQLS 194
N K+ W ++ + P W+E G+F+ + S
Sbjct: 36 NTKFESWAIEMAALHEPTWREAGIFEAVMAS 66
>At1g07710 hypothetical protein
Length = 543
Score = 27.7 bits (60), Expect = 4.0
Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
Query: 34 PSPSSTLR-----FQNIVHNSTKNFEIFMARVEGSSSQPTKQHEPNVNLPVNT 81
P+P+ L+ ++ VHN ++ + RV+G + Q K H +N +N+
Sbjct: 306 PNPARELKQTVSDIKHEVHNQLEHTRLTRKRVQGIAKQLNKMHTEGLNNAINS 358
>At2g43290 putative calcium binding protein
Length = 215
Score = 27.3 bits (59), Expect = 5.2
Identities = 33/118 (27%), Positives = 48/118 (39%), Gaps = 17/118 (14%)
Query: 18 IFTLTSFLLSLLLLFSPSPSSTLR----FQNIVHNSTKNFEIFMARVEGSSSQPTKQHEP 73
IF L + L S LL P TL F +H ++ M SSS PTK+ +P
Sbjct: 4 IFLLYNILNSFLLSLVPKKLRTLFPLSWFDKTLHKNSPPSPSTMLPSPSSSSAPTKRIDP 63
Query: 74 NVNLPVNTQFMEEPDVKEAEDKIWKSQVIISCPNSSHEYLG---PLSDESDDQKKLDA 128
+ V F + D + ++++ N S E LG P D + K+DA
Sbjct: 64 SELKRVFQMFDKNGDGRITKEEL----------NDSLENLGIYIPDKDLTQMIHKIDA 111
>At2g18900 unknown protein
Length = 804
Score = 27.3 bits (59), Expect = 5.2
Identities = 14/32 (43%), Positives = 21/32 (64%), Gaps = 2/32 (6%)
Query: 98 KSQVIISCPNSSHEYL--GPLSDESDDQKKLD 127
KSQ+ ++ N SHEY+ P SDES ++ +D
Sbjct: 692 KSQLRLAYVNGSHEYVVFDPNSDESHERSAVD 723
>At1g50790 hypothetical protein
Length = 812
Score = 27.3 bits (59), Expect = 5.2
Identities = 9/31 (29%), Positives = 17/31 (54%)
Query: 164 NEKYLDWLDKVEKIKGPFWKEMGVFDLIQLS 194
+ K+ W K+ + P W++ G+F+ I S
Sbjct: 61 HNKFNSWARKMSALHEPIWRKAGIFEAILAS 91
>At1g14440 unknown protein
Length = 312
Score = 27.3 bits (59), Expect = 5.2
Identities = 37/131 (28%), Positives = 58/131 (44%), Gaps = 24/131 (18%)
Query: 48 NSTKNFEIFMARVEG----SSSQPTKQHEPNVNLPVNTQFMEEPDVKEAEDKIWKSQVII 103
N +NF VEG ++ P QH P+ L +N Q + Q+I+
Sbjct: 130 NCHRNFH--RKEVEGELAATAMSPYHQHPPHRKLMLNHQKIRSA---------MPHQMIM 178
Query: 104 SCPNSSHEYLGPLSDESDDQKKLDAVFPWNEAQLPLIFSKGPYDLSFLKSKFRTEPKIEN 163
S++ Y+ S ES+D + D V + + LP + PY+ K +FRT+ E
Sbjct: 179 PIGVSNYRYMHNNS-ESEDFMEEDGVTTASRS-LPNL----PYNQ---KKRFRTKFTPEQ 229
Query: 164 NEKYLDWLDKV 174
EK L + +KV
Sbjct: 230 KEKMLSFAEKV 240
>At5g55720 pectate lyase
Length = 392
Score = 26.9 bits (58), Expect = 6.8
Identities = 17/57 (29%), Positives = 32/57 (55%), Gaps = 5/57 (8%)
Query: 44 NIVHNSTKNFEIFMARVEGSSSQPTKQHEPNVNLPVNTQFMEEPDVKEAE--DKIWK 98
+IV+N ++++++ G S+ PT + NV + N QF +E +E+ D+ WK
Sbjct: 279 HIVNNIYRDWKMYAI---GGSANPTIFSQGNVFIASNNQFTKEVTKRESADGDEEWK 332
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.317 0.134 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,667,077
Number of Sequences: 26719
Number of extensions: 205159
Number of successful extensions: 646
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 630
Number of HSP's gapped (non-prelim): 31
length of query: 194
length of database: 11,318,596
effective HSP length: 94
effective length of query: 100
effective length of database: 8,807,010
effective search space: 880701000
effective search space used: 880701000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC140773.5