BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140720.11 - phase: 0
(139 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g67620 unknown protein 171 1e-43
At5g62900 unknown protein 100 2e-22
At5g50090 unknown protein 97 2e-21
At1g60010 unknown protein 80 3e-16
At1g10530 unknown protein 77 4e-15
At1g29190 hypothetical protein 37 0.004
At1g29195 unknown protein 37 0.004
At4g28560 predicted GPI-anchored protein (fragment) 31 0.24
At5g03890 putative protein 30 0.41
At5g23650 putative protein 30 0.53
At3g14370 putative protein kinase 29 0.69
At2g37940 unknown protein 27 2.6
At3g31440 hypothetical protein 27 4.5
At1g35940 hypothetical protein 27 4.5
At5g34960 putative protein 26 5.9
At5g56930 unknown protein 26 7.7
At4g31570 putative protein 26 7.7
At3g10120 unknown protein 26 7.7
At2g14590 Mutator-like transposase 26 7.7
>At5g67620 unknown protein
Length = 182
Score = 171 bits (433), Expect = 1e-43
Identities = 85/118 (72%), Positives = 99/118 (83%), Gaps = 4/118 (3%)
Query: 1 MGNCQAAELATVVIHRPG-NKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPL 59
MGNCQAAE ATV+IH P NKVERIYWSV+A ++M SNPGHYVA+VV+SPT+K+E G+PL
Sbjct: 1 MGNCQAAEAATVLIHHPAENKVERIYWSVTASDIMKSNPGHYVAVVVTSPTMKNEKGLPL 60
Query: 60 KHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKESGNRGI---QMKHR 114
K LKLLRPDDTLLIG VYRL+SFE+VL EFA+KKC KLGKLLKE G + + KHR
Sbjct: 61 KQLKLLRPDDTLLIGHVYRLVSFEEVLNEFATKKCVKLGKLLKEGGGLDLTKKKTKHR 118
>At5g62900 unknown protein
Length = 161
Score = 100 bits (250), Expect = 2e-22
Identities = 52/100 (52%), Positives = 70/100 (70%), Gaps = 1/100 (1%)
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLK 60
MGNCQAAE AT VI +P K R Y +V+A EV+ S+PGH+VAL++SS + + +
Sbjct: 1 MGNCQAAEAATTVIQQPDGKSVRFYCTVNASEVIKSHPGHHVALLLSS-AVPHGGSLRVT 59
Query: 61 HLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKL 100
+KLLRP D LL+G VYRLIS E+V+K +KK GK+ K+
Sbjct: 60 RIKLLRPSDNLLLGHVYRLISSEEVMKGIRAKKSGKMKKI 99
>At5g50090 unknown protein
Length = 159
Score = 97.4 bits (241), Expect = 2e-21
Identities = 54/112 (48%), Positives = 73/112 (64%), Gaps = 5/112 (4%)
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSE---NGM 57
MGNCQA + A VVI P K E++ VSA VM NPGH V+L++S+ L S +G
Sbjct: 1 MGNCQAVDTARVVIQHPNGKEEKLSCPVSASYVMKMNPGHCVSLLISTTALSSASSGHGG 60
Query: 58 PLK--HLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKESGNR 107
PL+ +KLLRP DTL++G VYRLI+ ++V+K +KKC KL K K S ++
Sbjct: 61 PLRLTRIKLLRPTDTLVLGHVYRLITTKEVMKGLMAKKCSKLKKESKGSDDK 112
>At1g60010 unknown protein
Length = 173
Score = 80.1 bits (196), Expect = 3e-16
Identities = 44/116 (37%), Positives = 64/116 (54%), Gaps = 12/116 (10%)
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTL--------- 51
MGNCQA + A +V+ P K++R Y VS E+M PGHYV+L++ P
Sbjct: 1 MGNCQAVDAAALVLQHPDGKIDRYYGPVSVSEIMRMYPGHYVSLIIPLPEKNIPATTTTT 60
Query: 52 --KSENG-MPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKES 104
KSE + +KLLRP + L++G YRLI+ ++V+K +KK K K E+
Sbjct: 61 DDKSERKVVRFTRVKLLRPTENLVLGHAYRLITSQEVMKVLRAKKYAKTKKHQSET 116
>At1g10530 unknown protein
Length = 166
Score = 76.6 bits (187), Expect = 4e-15
Identities = 41/113 (36%), Positives = 58/113 (51%), Gaps = 14/113 (12%)
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVV-------------- 46
MGNCQA A +V+ PG ++R Y SVS EVM PGHYV+L++
Sbjct: 1 MGNCQAVNAAVLVLQHPGGIIDRYYSSVSVTEVMAMYPGHYVSLIIPLSEEEEKNIPATE 60
Query: 47 SSPTLKSENGMPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGK 99
K + ++LLRP + L++G YRLI+ ++V+K KK K K
Sbjct: 61 KGDDKKQRKAVRFTRVQLLRPTENLVLGHAYRLITSQEVMKVLREKKSAKTKK 113
>At1g29190 hypothetical protein
Length = 330
Score = 36.6 bits (83), Expect = 0.004
Identities = 30/104 (28%), Positives = 50/104 (47%), Gaps = 10/104 (9%)
Query: 13 VIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLKHLK--LLRPDDT 70
++H G+ VE I +++A E+M ++P H V SSPT + + K ++ P+
Sbjct: 23 IVHSNGH-VEEISGTITASEIMKAHPKH-VLKKPSSPTSDHDERDVISATKIVIVPPEAE 80
Query: 71 LLIGQVYRLISFEDVLKEFASKKCGKLGKLLKESGNRGIQMKHR 114
L G++Y L + S KC GK+ +E N +K R
Sbjct: 81 LQRGKIYFL------MPATKSDKCAGGGKIRREKSNANAVVKKR 118
>At1g29195 unknown protein
Length = 193
Score = 36.6 bits (83), Expect = 0.004
Identities = 30/104 (28%), Positives = 50/104 (47%), Gaps = 10/104 (9%)
Query: 13 VIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLKHLK--LLRPDDT 70
++H G+ VE I +++A E+M ++P H V SSPT + + K ++ P+
Sbjct: 23 IVHSNGH-VEEISGTITASEIMKAHPKH-VLKKPSSPTSDHDERDVISATKIVIVPPEAE 80
Query: 71 LLIGQVYRLISFEDVLKEFASKKCGKLGKLLKESGNRGIQMKHR 114
L G++Y L + S KC GK+ +E N +K R
Sbjct: 81 LQRGKIYFL------MPATKSDKCAGGGKIRREKSNANAVVKKR 118
>At4g28560 predicted GPI-anchored protein (fragment)
Length = 449
Score = 30.8 bits (68), Expect = 0.24
Identities = 23/73 (31%), Positives = 33/73 (44%), Gaps = 13/73 (17%)
Query: 56 GMPLKHLKLLRPDDTLLIGQVYRLISFEDVLKE-------------FASKKCGKLGKLLK 102
G LK+L L D+ L GQ+ F D +E F+ ++GK LK
Sbjct: 344 GSSLKNLCFLALDNNNLDGQIPEEFGFLDSAREINLENNNLTGKAPFSDSFRDRIGKKLK 403
Query: 103 ESGNRGIQMKHRD 115
SGN +Q+K+ D
Sbjct: 404 LSGNVNLQVKNSD 416
>At5g03890 putative protein
Length = 179
Score = 30.0 bits (66), Expect = 0.41
Identities = 23/80 (28%), Positives = 31/80 (38%), Gaps = 16/80 (20%)
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLK 60
MGNC E + I R KV +S H ++ GH + S N
Sbjct: 1 MGNCLVMEKKVIKIVRDDGKVLEYREPISVHHILTQFSGHSI----------SHNNTH-- 48
Query: 61 HLKLLRPDDTLLIGQVYRLI 80
L PD LL G++Y L+
Sbjct: 49 ----LLPDAKLLSGRLYYLL 64
>At5g23650 putative protein
Length = 337
Score = 29.6 bits (65), Expect = 0.53
Identities = 20/73 (27%), Positives = 34/73 (46%), Gaps = 4/73 (5%)
Query: 42 VALVVSSPTLKSENGMPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLL 101
+A VV TL+ ++H +L D L+ RL ++D L+E GK +L
Sbjct: 39 IATVVPGKTLEQV----IEHYNILARDVMLIESGCVRLPDYDDFLEEPNHNAFGKERSIL 94
Query: 102 KESGNRGIQMKHR 114
+ +R + KH+
Sbjct: 95 EGGNDRKYESKHK 107
>At3g14370 putative protein kinase
Length = 480
Score = 29.3 bits (64), Expect = 0.69
Identities = 20/66 (30%), Positives = 34/66 (51%), Gaps = 1/66 (1%)
Query: 27 SVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLKHLKLLRPDDTLLIGQVYRLISFEDVL 86
S S+ V + + + + S+ L S+ + L+HLKL+R T +G+V+ L + D
Sbjct: 53 STSSSSVNHRRHDPHWSAIKSAKLLSSDGNIHLRHLKLIRHLGTGNLGRVF-LCNLRDSS 111
Query: 87 KEFASK 92
FA K
Sbjct: 112 ARFALK 117
>At2g37940 unknown protein
Length = 305
Score = 27.3 bits (59), Expect = 2.6
Identities = 12/23 (52%), Positives = 15/23 (65%)
Query: 16 RPGNKVERIYWSVSAHEVMNSNP 38
R G+KV R+ W SA EV+ NP
Sbjct: 143 REGSKVSRLPWPKSALEVLEINP 165
>At3g31440 hypothetical protein
Length = 536
Score = 26.6 bits (57), Expect = 4.5
Identities = 18/62 (29%), Positives = 26/62 (41%)
Query: 45 VVSSPTLKSENGMPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKES 104
V + T+ G L+H+ L P GQ+Y +S K K GKL K++
Sbjct: 464 VAFAMTINKSQGQSLEHVGLYLPKPVFSHGQLYVALSRVTSKKGLKILILDKNGKLQKQT 523
Query: 105 GN 106
N
Sbjct: 524 TN 525
>At1g35940 hypothetical protein
Length = 1678
Score = 26.6 bits (57), Expect = 4.5
Identities = 18/62 (29%), Positives = 26/62 (41%)
Query: 45 VVSSPTLKSENGMPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKES 104
V + T+ G L+H+ L P GQ+Y +S K K GKL K++
Sbjct: 1606 VAFAMTINKSQGQSLEHIGLYLPKPVFSHGQLYVALSRVTSKKGLKILILDKDGKLQKQT 1665
Query: 105 GN 106
N
Sbjct: 1666 TN 1667
>At5g34960 putative protein
Length = 1033
Score = 26.2 bits (56), Expect = 5.9
Identities = 18/62 (29%), Positives = 27/62 (43%)
Query: 45 VVSSPTLKSENGMPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKES 104
V + T+ + G L+H+ L P GQ+Y +S K K GKL K++
Sbjct: 961 VAFAMTINTSQGQSLEHVGLYLPKAVFSHGQLYVALSRVTSKKGLKFLILDKDGKLQKQT 1020
Query: 105 GN 106
N
Sbjct: 1021 TN 1022
>At5g56930 unknown protein
Length = 675
Score = 25.8 bits (55), Expect = 7.7
Identities = 14/43 (32%), Positives = 26/43 (59%), Gaps = 2/43 (4%)
Query: 45 VVSSPTLKSENGMPLKHLKLLRPDDT--LLIGQVYRLISFEDV 85
V+SS L++ NG + ++L +P D +L+ V R++ +DV
Sbjct: 190 VISSGVLEACNGTVQREMELEKPVDNSPVLVDSVSRIVGGDDV 232
>At4g31570 putative protein
Length = 2712
Score = 25.8 bits (55), Expect = 7.7
Identities = 24/94 (25%), Positives = 41/94 (43%), Gaps = 14/94 (14%)
Query: 28 VSAHEVMNSNPGHYVALVVSSPTLKSENGMPLK------HLKLLRPDDTLLIGQVYRLIS 81
V+ EV NSN ++L+ T+ EN + L+ HL+L LI + Y L
Sbjct: 644 VTLQEVENSNMKETLSLLTRQQTMFEENNIHLREENEKAHLEL----SAHLISETYLLSE 699
Query: 82 FEDVLKEFASKKCGKLGKLLKESGNRGIQMKHRD 115
+ ++ + + KLLK G + ++ D
Sbjct: 700 YSNLKEGYTLLN----NKLLKFQGEKEHLVEEND 729
>At3g10120 unknown protein
Length = 173
Score = 25.8 bits (55), Expect = 7.7
Identities = 23/83 (27%), Positives = 32/83 (37%), Gaps = 12/83 (14%)
Query: 1 MGNCQAAELATVVIHRPGNKVERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLK 60
MGNC E + I R KV + H ++ HY +L S L
Sbjct: 1 MGNCLVMEKKVIKIMRNDGKVVEYRGPMKVHHILTQFSPHY-SLFDS-----------LT 48
Query: 61 HLKLLRPDDTLLIGQVYRLISFE 83
+ L P LL G++Y L+ E
Sbjct: 49 NNCHLHPQAKLLCGRLYYLLPQE 71
>At2g14590 Mutator-like transposase
Length = 323
Score = 25.8 bits (55), Expect = 7.7
Identities = 18/54 (33%), Positives = 26/54 (47%), Gaps = 4/54 (7%)
Query: 88 EFASKKCGKLGKLLKESGNRGIQMKHRDFRAPNPSPV----KVIKFHVNVNVTE 137
EFA + K K+L+E NR I++ R N +P+ V+ H N N E
Sbjct: 231 EFAMWRENKQAKMLREMQNREIRLAGSLGRQVNSTPINVQDNVVGGHSNSNPEE 284
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,332,431
Number of Sequences: 26719
Number of extensions: 137516
Number of successful extensions: 310
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 294
Number of HSP's gapped (non-prelim): 19
length of query: 139
length of database: 11,318,596
effective HSP length: 89
effective length of query: 50
effective length of database: 8,940,605
effective search space: 447030250
effective search space used: 447030250
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC140720.11